EcoliWiki - User contributions [en]

pCR2.1

2010-06-23T16:13:41Z

2010-06-15T20:00:01Z

Gwen: Updating New_Quickview_Table table.

dmlR:Quickview

2010-06-15T20:00:00Z

Gwen: Updating New_Quickview_Table table.

PMID:18950999

2010-03-22T20:50:00Z

Gwen: Fill PMID: Page!

{{RightTOC}}
<protect>
 '''Froese, DS, Dobson, CM, White, AP, Wu, X, Padovani, D, Banerjee, R, Haller, T, Gerlt, JA, Surette, MG and Gravel, RA''' (2009) Sleeping beauty mutase (sbm) is expressed and interacts with ygfd in Escherichia coli.''Microbiol. Res.'' '''164''':1-8

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>In Escherichia coli, a four-gene operon, sbm-ygfD-ygfG-ygfH, has been shown to encode a putative cobalamin-dependent pathway with the ability to produce propionate from succinate in vitro [Haller T, Buckel T, Retey J, Gerlt JA. Discovering new enzymes and metabolic pathways: conversion of succinate to propionate by Escherichia coli. Biochemistry 2000;39:4622-4629]. However, the operon was thought to be silent in vivo, illustrated by the eponym describing its first gene, "sleeping beauty mutase" (methylmalonyl-CoA mutase, MCM). Of the four genes described, only ygfD could not be assigned a function. In this study, we have evaluated the functional integrity of YgfD and Sbm and show that, indeed, both proteins are expressed in E. coli and that YgfD has GTPase activity. We show that YgfD and Sbm can be co-immunoprecipitated from E. coli extracts using antibody to either protein, demonstrating in vivo interaction, a result confirmed using a strain deleted for ygfD. We show further that, in vitro, purified His-tagged YgfD and Sbm behave as a monomer and dimer, respectively, and that they form a multi-subunit complex that is dependent on pre-incubation of YgfD with non-hydrolysable GTP, an outcome that was not affected by the state of Sbm, as holo- or apoenzyme. These studies reinforce a role for the in vivo interaction of YgfD and Sbm.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18950999 PubMed] Online version:[http://dx.doi.org/10.1016/j.micres.2008.08.006 10.1016/j.micres.2008.08.006]
|}
</protect>
==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="A4ba7d7f86a320" class=" tableEdit GO_table_reference"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.171691.A4ba7d7f86a320&page=171691&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:18950999

2010-03-22T20:49:59Z

Gwen: New PMID: Page!

argK:Gene Product(s)

2010-03-22T20:49:46Z

Gwen: Table edited by Gwen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|argK}}{{Tab|Gene|argK}}{{Tab On|Gene Product(s)|argK}}{{Tab|Expression|argK}}{{Tab|Evolution|argK}}{{Tab|On One Page|argK}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==

{{Product_nomenclature_help}}
{| id="K46aac6de446b0" class=" tableEdit Product_nomenclature_table"

|-
!align=left {{table heading style}} |Standard name
||
ArgK
|-
!align=left {{table heading style}} |Synonyms
||
membrane ATPase/protein kinase<ref name='LIB:Riley_2006'/>, B2918<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YgfD<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, ArgK<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
arginine transport ATPase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

Required to convert succinate to propionate, Arg transport?; reported to have ATPase and protein kinase activity<ref name='LIB:EcoGene'/>
|-
!align=left {{table heading style}} |EC number (for enzymes)
||

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26213.K46aac6de446b0&page=26213&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}


<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> 
{{Product_GO_help}}
{| id="V46aac6df55cd9" class=" tableEdit GO_table_product dataTable"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
GO:0000166
|
nucleotide binding
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0547
|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|

|
GO:0005524
|
ATP binding
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0067
|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|

|
GO:0006810
|
transport
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0813
|
P
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|

|
GO:0016020
|
membrane
|
GO_REF:0000004 GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0397 SP_KW:KW-0472
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
GO:0016301
|
kinase activity
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0418
|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|

|
GO:0016740
|
transferase activity
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0808
|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|

|
GO:0003924
|
GTPase activity
|
PMID:18950999
|
IDA: Inferred from Direct Assay
|

|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|

|
GO:0005515
|
protein binding
|
PMID:18950999
|
IDA: Inferred from Direct Assay
|
UniProtKB:P27253
|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|

|
GO:0005886
|
plasma membrane
|
GO_REF:0000004 GO_REF:0000004 GO_REF:0000023
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0997 SP_KW:KW-1003 SP_SL:SL-0037
|
C
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26213.V46aac6df55cd9&page=26213&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>

{{Product_interactions_help}}
{| id="O46aac6e2bb876" class=" tableEdit Product_interactions_table dataTable"
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References!!Evidence
|-
|
Protein
|
rplB 
|

|
PMID:16606699
|
Experiment(s):EBI-1144302 
|-
|
Protein
|
yphE 
|

|
PMID:16606699
|
Experiment(s):EBI-1144302 
|-
|
Protein
|
serA 
|

|
PMID:16606699
|
Experiment(s):EBI-1144302 
|-
|
Protein
|
groL 
|

|
PMID:16606699
|
Experiment(s):EBI-1144302 
|-
|
Protein
|
sbm
|

|
PMID:18950999
|
Direct/co-IP

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26213.O46aac6e2bb876&page=26213&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table] || || || ||
|}


</protect>
===Notes===

==Localization==

{{Product_localization_help}}
{| id="H46aac6e383cae" class=" tableEdit Product_localization_table"
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Source!!Notes
|-
|
cytoplasm
|

|

|

|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26213.H46aac6e383cae&page=26213&pagename={{FULLPAGENAMEE}}&type=&template=Product_localization_table edit table] || || || ||
|}


<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>

{{Product_physical_properties_help}}
{| id="W46aac6e5244ea" class=" tableEdit Product_phys_prop_table"

|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=EG11445-MONOMER at EcoCyc]
<pre>MINEATLAES IRRLRQGERA TLAQAMTLVE SRHPRHQALS TQLLDAIMPY CGNTLRLGVT
GTPGAGKSTF LEAFGMLLIR EGLKVAVIAV DPSSPVTGGS ILGDKTRMND LARAEAAFIR
PVPSSGHLGG ASQRARELML LCEAAGYDVV IVETVGVGQS ETEVARMVDC FISLQIAGGG
DDLQGIKKGL MEVADLIVIN KDDGDNHTNV AIARHMYESA LHILRRKYDE WQPRVLTCSA
LEKRGIDEIW HAIIDFKTAL TASGRLQQVR QQQSVEWLRK QTEEEVLNHL FANEDFDRYY
RQTLLAVKNN TLSPRTGLRQ LSEFIQTQYF D</pre>
|-
!align=left {{table heading style}} |Length
||
331
|-
!align=left {{table heading style}} |Mol. Wt
||
36.704 kDa
|-
!align=left {{table heading style}} |pI
||
6.5 (calculated)
|-
!align=left {{table heading style}} |Extinction coefficient
||
26,930 - 27,430 (calc based on 7 Y, 3 W, and 4 C residues)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26213.W46aac6e5244ea&page=26213&pagename={{FULLPAGENAMEE}}&type=1&template=Product_phys_prop_table edit table] ||
|}


<big>'''Domains/Motifs/Modification Sites'''</big><table>
<tr align="left" valign="top">
<td>
{{Product_domains_motifs_help}}
{| id="Y46aac6e594db0" class=" tableEdit Product_motif_table dataTable"
|- {{table heading style}}
!|Type!!Residues!!Description!!Notes!!References

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26213.Y46aac6e594db0&page=26213&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table] || || || ||
|}
</td>
<td>


<motif_map/>
</td>
</tr>
</table>

<table><tr valign='top'><td>
<big>'''Structure'''</big> 

{{Product_structure_help}}
{| id="J46a85c6c86247" class=" tableEdit Product_structure_table"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>
gene=argK
taxon=562,83333
</beststructure>
|-
!align=left {{table heading style}} |Models
||
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26213.J46a85c6c86247&page=26213&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table] ||
|}


</td><td></protect>
==== Structure figures ====

<protect>
</td><tr><table></protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}

{{Product_resources_help}}
{| id="E46aac6e6601ff" class=" tableEdit Product_resource_table"
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26213.E46aac6e6601ff&page=26213&pagename={{FULLPAGENAMEE}}&type=&template=Product_resource_table edit table] || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="D46aac6e6bf3bb" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11445-MONOMER EcoCyc:EG11445-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P27254|UniProt:P27254]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF03308 PFAM:PF03308]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417393 RefSeq:NP_417393]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26213.D46aac6e6bf3bb&page=26213&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="S46a85c70099ec" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26213.S46a85c70099ec&page=26213&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0006810_!_transport]][[Category:Location:cytoplasm]]

scpB:Gene Product(s)

2010-03-22T20:47:17Z

Gwen: Table edited by Gwen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|scpB}}{{Tab|Gene|scpB}}{{Tab On|Gene Product(s)|scpB}}{{Tab|Expression|scpB}}{{Tab|Evolution|scpB}}{{Tab|On One Page|scpB}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==

{{Product_nomenclature_help}}
{| id="E46aac6eb3cb38" class=" tableEdit Product_nomenclature_table"

|-
!align=left {{table heading style}} |Standard name
||
YgfG
|-
!align=left {{table heading style}} |Synonyms
||
methylmalonyl-CoA decarboxylase, biotin-independent<ref name='LIB:Riley_2006'/>, B2919<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YgfG<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
YgfG<ref name='LIB:EcoCyc10.6'/>; ScpB<ref name='LIB:EcoCyc11.1'/>;

Component of [[:Category:Complex:methylmalonyl-CoA decarboxylase|methylmalonyl-CoA decarboxylase]]<ref name='LIB:EcoCyc11.1'/>

Methylmalonyl-CoA decarboxylase<ref name='LIB:EcoGene'/>
|-
!align=left {{table heading style}} |EC number (for enzymes)
||
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.41 4.1.1.41]<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26215.E46aac6eb3cb38&page=26215&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}


<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> 
{{Product_GO_help}}
{| id="J46aac6eca8102" class=" tableEdit GO_table_product dataTable"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
GO:0016829
|
lyase activity
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0456
|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|

|
GO:0008152
|
metabolic process
|
GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR001753
|
P
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|

|
GO:0004492
|
methylmalonyl-CoA decarboxylase activity
|
GO_REF:0000003
|
IEA: Inferred from Electronic Annotation
|
EC:4.1.1.41
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
GO:0003824
|
catalytic activity
|
GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR001753
|
F
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
|
complete
|-
|

|
GO:0005737
|
cytoplasm
|

|

|

|
C
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
GO:0004492
|

|
PMID:10769117
|
IDA: Inferred from Direct Assay
|

|

|

|
complete

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26215.J46aac6eca8102&page=26215&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>

{{Product_interactions_help}}
{| id="L46aac6eec92b8" class=" tableEdit Product_interactions_table dataTable"
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References!!Evidence
|-
|
Protein
|
Subunits of [[:Category:Complex:methylmalonyl-CoA decarboxylase|methylmalonyl-CoA decarboxylase]]
|
could be indirect
|

|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26215.L46aac6eec92b8&page=26215&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table] || || || ||
|}


</protect>
===Notes===

==Localization==

{{Product_localization_help}}
{| id="Y46a85c75432a4" class=" tableEdit Product_localization_table"
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Source!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26215.Y46a85c75432a4&page=26215&pagename={{FULLPAGENAMEE}}&type=&template=Product_localization_table edit table] || || || ||
|}


<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>

{{Product_physical_properties_help}}
{| id="Q46aac6f1314b1" class=" tableEdit Product_phys_prop_table"

|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=G7516-MONOMER at EcoCyc]
<pre>MNLSRRNILI KGIFMSYQYV NVVTINKVAV IEFNYGRKLN ALSKVFIDDL MQALSDLNRP
EIRCIILRAP SGSKVFSAGH DIHELPSGGR DPLSYDDPLR QITRMIQKFP KPIISMVEGS
VWGGAFEMIM SSDLIIAAST STFSMTPVNL GVPYNLVGIH NLTRDAGFHI VKELIFTASP
ITAQRALAVG ILNHVVEVEE LEDFTLQMAH HISEKAPLAI AVIKEELRVL GEAHTMNSDE
FERIQGMRRA VYDSEDYQEG MNAFLEKRKP NFVGH</pre>
|-
!align=left {{table heading style}} |Length
||
275
|-
!align=left {{table heading style}} |Mol. Wt
||
30.83 kDa
|-
!align=left {{table heading style}} |pI
||
6.6 (calculated)
|-
!align=left {{table heading style}} |Extinction coefficient
||
15,930 - 16,055 (calc based on 7 Y, 1 W, and 1 C residues)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26215.Q46aac6f1314b1&page=26215&pagename={{FULLPAGENAMEE}}&type=1&template=Product_phys_prop_table edit table] ||
|}


<big>'''Domains/Motifs/Modification Sites'''</big><table>
<tr align="left" valign="top">
<td>
{{Product_domains_motifs_help}}
{| id="B46aac6f1a160c" class=" tableEdit Product_motif_table dataTable"
|- {{table heading style}}
!|Type!!Residues!!Description!!Notes!!References

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26215.B46aac6f1a160c&page=26215&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table] || || || ||
|}
</td>
<td>


<motif_map/>
</td>
</tr>
</table>

<table><tr valign='top'><td>
<big>'''Structure'''</big> 

{{Product_structure_help}}
{| id="T46aac6f20bfb0" class=" tableEdit Product_structure_table"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>
gene=scpB
taxon=562,83333
</beststructure>
|-
!align=left {{table heading style}} |Models
||
View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=recA EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=recA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26215.T46aac6f20bfb0&page=26215&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table] ||
|}


</td><td></protect>
==== Structure figures ====

<protect>
</td><tr><table></protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}

{{Product_resources_help}}
{| id="H46aac6f3837f5" class=" tableEdit Product_resource_table"
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26215.H46aac6f3837f5&page=26215&pagename={{FULLPAGENAMEE}}&type=&template=Product_resource_table edit table] || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="H46aac6f3e2fc1" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G7516-MONOMER EcoCyc:G7516-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P52045|UniProt:P52045]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF00378 PFAM:PF00378]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417394 RefSeq:NP_417394]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26215.H46aac6f3e2fc1&page=26215&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="A46a85c7c16774" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.26215.A46a85c7c16774&page=26215&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
{{BacktoTop}}
[[Category:GO:0005737_!_cytoplasm]][[Category:Complex:methylmalonyl-CoA decarboxylase]][[Category:Products with structures in PDB]]
[[Category:Proteins]]

TubercuList

2009-09-18T20:36:08Z

Gwen: Table edited by Gwen via TableEdit

{{Pagetop}}
[[Category:Databases]]
[[Category:Bacterial Databases]]
<protect>

{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}} width='30%' align="right"

|-
!align=left {{table heading style}} |Link/URL:
||
http://tuberculist.epfl.ch/
|-
!align=left {{table heading style}} |What:
||
TubercuList provides a complete dataset of DNA and protein sequences derived from the strain ''M. tuberculosis'' H37Rv.
|-
!align=left {{table heading style}} |Who:
||
The laboratory of Stewart cole
|-
!align=left {{table heading style}} |Updates:
||
September 2009
|-
!align=left {{table heading style}} |Upcoming events:
||

|-
!align=left {{table heading style}} |Web Services:
||

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.135198.I489b574022695&page=135198&pagename={{FULLPAGENAMEE}}&type=1&template=Database_table edit table] ||
|}
</protect>
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
''Add general information here''
[{{{1|link}}} {{PAGENAME}}] is...
==Content {{HelpIcon|Help:Databases}}==

==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==
===Browsing===
===Searching===
===Usage examples===
''Add links to additional pages describing success stories here.''

==Other sites with related content {{HelpIcon|Help:Databases}}==

==Technology {{HelpIcon|Help:Databases}}==
===Web Services/API===

==Discussion {{HelpIcon|Help:Databases}}==

==References {{HelpIcon|Help:Databases}}==
<references />

==External Links==
[{{{1|http://tuberculist.epfl.ch//}}}
{{PAGENAME}}] URL:{{{1|http://tuberculist.epfl.ch/}}}
===Discussion of {{PAGENAME}} on other websites===

TubercuList

2009-09-18T20:35:05Z

Gwen: /* External Links */

{{Pagetop}}
[[Category:Databases]]
[[Category:Bacterial Databases]]
<protect>

{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}} width='30%' align=right

|-
!align=left {{table heading style}} |Link/URL:
||
http://genolist.pasteur.fr/TubercuList
|-
!align=left {{table heading style}} |What:
||
TubercuList provides a complete dataset of DNA and protein sequences derived from the strain ''M. tuberculosis'' H37Rv.
|-
!align=left {{table heading style}} |Who:
||
The Unit for Genetique Moleculaire Bacterienne
|-
!align=left {{table heading style}} |Updates:
||
July 1, 2008
|-
!align=left {{table heading style}} |Upcoming events:
||

|-
!align=left {{table heading style}} |Web Services:
||

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.135198.I489b574022695&page=135198&pagename={{FULLPAGENAMEE}}&type=1&template=Database_table edit table] ||
|}
</protect>
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
''Add general information here''
[{{{1|link}}} {{PAGENAME}}] is...
==Content {{HelpIcon|Help:Databases}}==

==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==
===Browsing===
===Searching===
===Usage examples===
''Add links to additional pages describing success stories here.''

==Other sites with related content {{HelpIcon|Help:Databases}}==

==Technology {{HelpIcon|Help:Databases}}==
===Web Services/API===

==Discussion {{HelpIcon|Help:Databases}}==

==References {{HelpIcon|Help:Databases}}==
<references />

==External Links==
[{{{1|http://tuberculist.epfl.ch//}}}
{{PAGENAME}}] URL:{{{1|http://tuberculist.epfl.ch/}}}
===Discussion of {{PAGENAME}} on other websites===

PMID:12615322

2009-08-26T17:32:14Z

Gwen: /* Useful Materials and Methods {{HelpIcon|Help:Literature}} */

{{RightTOC}}

 '''Loyevsky, M, Mompoint, F, Yikilmaz, E, Altschul, SF, Madden, T, Wootton, JC, Kurantsin-Mills, J, Kassim, OO, Gordeuk, VR and Rouault, TA''' (2003) Expression of a recombinant IRP-like Plasmodium falciparum protein that specifically binds putative plasmodial IREs.''Mol. Biochem. Parasitol.'' '''126''':231-8

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || Plasmodium falciparum iron regulatory-like protein (PfIRPa, accession AJ012289) has homology to a family of iron-responsive element (IRE)-binding proteins (IRPs) found in different species. We have previously demonstrated that erythrocyte P. falciparum PfIRPa binds a mammalian consensus IRE and that the binding activity is regulated by iron status. In the work we now report, we have cloned a C-terminus histidine-tagged PfIRPa and overexpressed it in a bacterial expression system in soluble form capable of binding IREs. To overexpress PfIRPa, we used the T7 promoter-driven vector, pET28a(+), in conjunction with the Rosetta(DE3)pLysS strain of E. coli, which carries extra copies of tRNA genes usually found in organisms such as P. falciparum whose genome is (A+T)-rich. The histidine-tagged recombinant protein (rPfIRPa) in soluble form was partially purified using His-bind resin. We searched the plasmodial database, plasmoDB, to identify sequences capable of forming IRE loops using a specially developed algorithm, and found three plasmodial sequences matching the search criteria. In gel retardation assays, rPfIRPa bound three 32P-labeled putative plasmodial IREs with affinity exceeding the affinity for the mammalian consensus IRE. The binding was concentration-dependent and was not inhibited by heparin, an inhibitor of non-specific binding. Immunodepletion of rPfIRPa resulted in substantial inhibition of the signal intensity in the gel retardation assays and in Western blot-determinations of rPfIRPa protein levels. Endogenous PfIRPa retained all three putative 32P-IREs at the same position on the gel as the recombinant PfIRPa.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12615322 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}} 
[[pET-28a(%2B)]]

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.A472ff85b5c205&page=0&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

User:Gwen

2009-07-24T14:19:22Z

Gwen:

__NOTOC__
[[Image:IMG_0019.jpg|thumb|right|Gwen on her defense day]]
Hi. My name is Gwen Knapp and I'm a former graduate student of [http://ecoliwiki.net/colipedia/index.php/User:JimHu Jim Hu's] at [http://biochemistry.tamu.edu Texas A&M University]. Currently, I am a post-doc in Kathleen McDonough's lab at the Wadsworth Center in Albany, NY, studying the ''Mycobacteria'' response to CO2. However, I still come back to EcoliWiki as a starting point as a genetic resource.

==Contact==
Gwen Knapp 
Room 4089 DAI 
120 New Scotland Ave 
Albany, NY 12208 
gwen.knapp@gmail.com 

Present work address: New York State Department of Health, Wadsworth Center, 120 New Scotland Ave. Albany, NY 12208
==Education==
1999 B.S. Purdue University, Molecular and Genetic Biology 
2008 PhD. Texas A&M University, Biochemistry

==Categories==
[[Category:Users who can help you get an account]]

cydB:Quickview

2009-02-03T21:27:29Z

Gwen: Table edited by Gwen via TableEdit

EcoliWiki:Wishlist

2008-10-31T14:22:10Z

Gwen: /* What we would like to see? */

==Wishlist==
The EcoliWiki team strives to make the Wiki better. To that end, we are asking for your opinion. If there is a feature not currently available in EcoliWiki that you would like to see, describe it here. Extended discussion of different requested features can go on the [[EcoliWiki_talk:Wishlist|discussion page]].

===Subunit Composition===
* Component term could be more descriptive with addition of subunit compositions (ie homomultimer/heteromultimer)

===What's up and coming for EcoliWiki v2===
*Searching for information present in the tables specifically
*If I search for multiple genes in EcoliWiki, it would be nice for the output to be given in some delimited form.

===What we would like to see?===
*Search and type greek and non-English characters or some toolbar for it (lambda vs. the symbol, delta, sigma, etc.)
*Search for phenotype and output is a list of genes
*References- Pubmed Central IDs (for things w/o PMIDs)
*A "Go To" alphabet to organize the category pages
* RNAs in W3110
** links to RFAM
** pulldown menus for RNAs in Gbrowse
*synthetic lethal database[[User:Smrlab|Smrlab]]
*interactome data base[[User:Smrlab|Smrlab]]-Butland, Mori, other studies you had in mind
*Have the "edit toolbar" visible when editing tables
*Info on or list of compounds that can be taken up by coli e.g. cAMP?
*Make TMHMM available to users on this site
*Promoter prediction tools
*A tool to map genes and/or proteins from a large data set onto pathways.

== Done ==
*Make TMHMM predictions and put that information on the gene products page
*Find based on three letter gene name prefix
*List of [[Essential genes]]
*CGSC Strain list - we don't have plans to enter all the strains, but we now have all the mutant alleles from CGSC in the allele tables, along with links to CGSC.
*References- Automatic DOI's for ''all'' PMID articles. DOI's are available from PubMed/MEDLINE and hopefully can be automatically imported when the article abstract is imported. Prepending "<nowiki>http://dx.doi.org/</nowiki>" to a DOI gives a URL that takes the user directly to the journal's version of the online article. See, e.g, the Links section of the table on the [[PMID:17244358]] page.
** DOI links will be created on all new PMID pages.
*Video clip upload
** Not exactly upload, but you can upload to YouTube or Google Video and embed the videos here.

pCR2.1

2008-10-22T00:56:37Z

Gwen:

==At a Glance {{HelpIcon|Help:Plasmid}}==
<protect></protect>
 <protect>

{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left |Type
||
Cloning
|-
!align=left |Origin
||
F1, pUC
|-
!align=left |Copy Number
||

|-
!align=left |Markers
||
amp, kan
|-
!align=left |Link to Sequence
||
[http://tools.invitrogen.com/content/sfs/vectors/pcr2_1_seq.txt Invitrogen]
|-
!align=left |Feature
||

|-
!align=left |Accession Number
||

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.140255.U48c6a4edd8d12&page=140255&pagename={{FULLPAGENAMEE}}&type=1&template= edit table] ||
|}
</protect>

 
===Notes===

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources {{HelpIcon|Help:Plasmid}}==
{{PlasmidSources}}

==References==
{{RefHelp}}
<references/>

==Sequence {{HelpIcon|Help:Plasmid}}==
{{PlasmidSeq}}

AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTT
TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAG
GCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAA
ACAGCTATGACCATGATTACGCCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGC
TGGAATTCGGCTTAAGCCGAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAG
GGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGA
AAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAA
GAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGG
CGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCC
GCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGG
GGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGG
TTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAAT
AGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGA
TTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAA
AATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGAC
CCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGC
TTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCA
GCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGA
TCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGA
TGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACA
ATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCG
ACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGT
TCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCG
GGGCAGGATCTCCTGTCATCCCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGC
GGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACG
TACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCC
GAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCT
GCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGC
GGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGAC
CGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACG
AGTTCTTCTGAATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGT
TGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGA
AGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAG
AAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACAC
TGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACA
CCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTC
CCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGG
GGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACG
AAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCA
TATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATA
ATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAA
AGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCA
GCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGC
AGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCC
TACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGG
TTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGC
CCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCT
TCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAG
CTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAT
TTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCT
GGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATT
ACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGG
AAGCGGAAG

[[Category:Plasmids]][[Category:Invitrogen plasmids]]
__NOTOC__

pCR2.1

2008-09-09T16:34:14Z

Gwen: /* Sequence {{HelpIcon|Help:Plasmid}} */

==At a Glance {{HelpIcon|Help:Plasmid}}==
<protect></protect>
 <protect>

{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left |Type
||
Cloning
|-
!align=left |Origin
||
F1, pUC
|-
!align=left |Copy Number
||

|-
!align=left |Markers
||
amp, kan
|-
!align=left |Link to Sequence
||
[http://tools.invitrogen.com/content/sfs/vectors/pcr2_1_seq.txt Invitrogen]
|-
!align=left |Feature
||

|-
!align=left |Accession Number
||

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.140255.U48c6a4edd8d12&page=140255&pagename={{FULLPAGENAMEE}}&type=1&template= edit table] ||
|}
</protect>

 
===Notes===

==History {{HelpIcon|Help:Plasmid}}==
{{PlasmidHistory}}

==Sources {{HelpIcon|Help:Plasmid}}==
{{PlasmidSources}}

==References==
{{RefHelp}}
<references/>

==Sequence {{HelpIcon|Help:Plasmid}}==
{{PlasmidSeq}}

AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTT
TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAG
GCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAA
ACAGCTATGACCATGATTACGCCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGC
TGGAATTCGGCTTAAGCCGAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAG
GGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGA
AAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAA
GAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGG
CGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCC
GCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGG
GGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGG
TTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAAT
AGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGA
TTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAA
AATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGAC
CCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGC
TTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCA
GCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGA
TCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGA
TGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACA
ATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCG
ACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGT
TCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCG
GGGCAGGATCTCCTGTCATCCCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGC
GGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACG
TACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCC
GAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCT
GCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGC
GGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGAC
CGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACG
AGTTCTTCTGAATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGT
TGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGA
AGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAG
AAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACAC
TGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACA
CCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTC
CCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGG
GGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACG
AAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCA
TATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATA
ATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAA
AGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCA
GCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGC
AGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCC
TACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGG
TTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGC
CCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCT
TCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAG
CTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAT
TTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCT
GGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATT
ACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGG
AAGCGGAAG

[[Category:Plasmids]]
__NOTOC__

pCR2.1

2008-09-09T16:34:05Z

Gwen: Table edited by Gwen via TableEdit

Gwen: Table edited by Gwen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|cbl}}{{Tab|Gene|cbl}}{{Tab On|Gene Product(s)|cbl}}{{Tab|Expression|cbl}}{{Tab|Evolution|cbl}}{{Tab|On One Page|cbl}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|{{Prettytable}}
|-
!{{table heading style}} |Standard name
|
Cbl product
|-
!{{table heading style}} |Synonyms
|
DNA-binding transcriptional activator of cysteine biosynthesis product<ref name='LIB:Riley_2006'/>, B1987 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Cbl product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!{{table heading style}} |Product description
|
Cbl transcriptional activator<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

|-
!{{table heading style}} |EC number (for enzymes)
|
*
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.F46aa8abc19c36&page=24094&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big>


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
NOT
|
[[Category:GO:0006790_!_sulfur_metabolic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006790_!_sulfur_metabolic_process GO:0006790]
|
sulfur metabolic process
|

|

|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required field missing
|-
|

|
[[Category:GO:0006350_!_transcription]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006350_!_transcription GO:0006350]
|
transcription
|
[[PMID:10506196]]<ref name=PMID:10506196/>
|
IDA: Inferred from Direct Assay
|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
complete
|-
|
NOT
|
[[Category:GO:0019344_!_cysteine_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0019344_!_cysteine_biosynthetic_process GO:0019344]
|
cysteine biosynthetic process
|

|

|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required field missing
|-
|

|
[[Category:GO:0016564_!_transcription_repressor_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016564_!_transcription_repressor_activity GO:0016564]
|
transcription repressor activity
|

|

|

|
F
|
Seeded from EcoCyc 10.6<ref name='LIB:EcoCyc10.6'/>.
|
required fields missing
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|

|

|

|
C
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0016563_!_transcription_activator_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016563_!_transcription_activator_activity GO:0016563]
|
transcription activator activity
|
[[PMID:10506196]]<ref name=PMID:10506196/>
|
IDA: Inferred from Direct Assay
|

|
F
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.F46aa8abd22da0&page=24094&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}

{{Product_GO_help}}

<big>'''Interactions'''</big>


{|class = 'sortable' {{Prettytable}} id='166083'
|- {{table heading style}}
!Partner Type!!Partner!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.J46aa8ad67ea6a&page=24094&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' {{Prettytable}} id='112522'
|- {{table heading style}}
!Compartment!!Description!!Evidence!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.N46a81e0a21360&page=24094&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|{{Prettytable}}
|-
!{{table heading style}} |Sequence
|
[http://biocyc.org/ECOLI/sequence-aa?object=G7071-MONOMER at EcoCyc]
<pre>MNFQQLKIIR EAARQDYNLT EVANMLFTSQ SGVSRHIREL EDELGIEIFV RRGKRLLGMT
EPGKALLVIA ERILNEASNV RRLADLFTND TSGVLTIATT HTQARYSLPE VIKAFRELFP
EVRLELIQGT PQEIATLLQN GEADIGIASE RLSNDPQLVA FPWFRWHHSL LVPHDHPLTQ
ISPLTLESIA KWPLITYRQG ITGRSRIDDA FARKGLLADI VLSAQDSDVI KTYVALGLGI
GLVAEQSSGE QEEENLIRLD TRHLFDANTV WLGLKRGQLQ RNYVWRFLEL CNAGLSVEDI
KRQVMESSEE EIDYQI</pre>
|-
!{{table heading style}} |Mol. Wt
|
35.86 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |pI
|

|-
!{{table heading style}} |Extinction coefficient
|
36440 - 36565 (calc based on 6 Y, 5 W, and 1 C residues)
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.G46aa8ad8633e7&page=24094&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


{|class = 'sortable' {{Prettytable}} id='166085'
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.S46aa8ad8cf9d2&page=24094&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|class = 'sortable' {{Prettytable}} id='112523'
|- {{table heading style}}
!Structure Type!!Organism!!Accession!!Residues covered!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.L46a81e0be2589&page=24094&pagename={{FULLPAGENAMEE}} edit table] || || || ||
|}

{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='166086'
|- {{table_heading_style}}
!Resource type!!Source!!Notes/Reference
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.X46aa8ad99468b&page=24094&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='166087'
|- {{table_heading_style}}
!Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G7071-MONOMER EcoCyc:G7071-MONOMER]
|

|-
|
[http://swissmodel.expasy.org/repository/ SwissModel] ([[SwissModel|EcoliWiki Page]])
|
[http://swissmodel.expasy.org/repository/smr.php?job=2&sptr_ac=Q47083 SwissModel:Q47083]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.expasy.ch/cgi-bin/sprot-search-ac?Q47083 UniProt:Q47083]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF00126 PFAM:PF00126]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_416492 RefSeq:NP_416492]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=Q47083 ModBase:Q47083]
|

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.N46aa8ad9f087d&page=24094&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='112524'
|- {{table_heading_style}}
!Name!!URL!!Comments
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.F46a81e104b863&page=24094&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0006790_!_sulfur metabolic process]][[Category:GO:0006350_!_transcription]][[Category:GO:0019344_!_cysteine biosynthetic process]][[Category:GO:0016564_!_transcription repressor activity]][[Category:GO:0005737_!_cytoplasm]][[Category:GO:0016563_!_transcription activator activity]]

cbl:Gene Product(s)

2008-07-17T20:52:16Z

Gwen: Table edited by Gwen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|cbl}}{{Tab|Gene|cbl}}{{Tab On|Gene Product(s)|cbl}}{{Tab|Expression|cbl}}{{Tab|Evolution|cbl}}{{Tab|On One Page|cbl}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|{{Prettytable}}
|-
!{{table heading style}} |Standard name
|
Cbl product
|-
!{{table heading style}} |Synonyms
|
DNA-binding transcriptional activator of cysteine biosynthesis product<ref name='LIB:Riley_2006'/>, B1987 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Cbl product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!{{table heading style}} |Product description
|
Cbl transcriptional activator<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

|-
!{{table heading style}} |EC number (for enzymes)
|
*
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.F46aa8abc19c36&page=24094&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big>


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0006790_!_sulfur_metabolic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006790_!_sulfur_metabolic_process GO:0006790]
|
sulfur metabolic process
|

|

|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0006350_!_transcription]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006350_!_transcription GO:0006350]
|
transcription
|
[[PMID:10506196]]<ref name=PMID:10506196/>
|
IDA: Inferred from Direct Assay
|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
complete
|-
|

|
[[Category:GO:0019344_!_cysteine_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0019344_!_cysteine_biosynthetic_process GO:0019344]
|
cysteine biosynthetic process
|

|

|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0016564_!_transcription_repressor_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016564_!_transcription_repressor_activity GO:0016564]
|
transcription repressor activity
|

|

|

|
F
|
Seeded from EcoCyc 10.6<ref name='LIB:EcoCyc10.6'/>.
|
required fields missing
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|

|

|

|
C
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0016563_!_transcription_activator_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016563_!_transcription_activator_activity GO:0016563]
|
transcription activator activity
|
[[PMID:10506196]]<ref name=PMID:10506196/>
|
IDA: Inferred from Direct Assay
|

|
F
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.F46aa8abd22da0&page=24094&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}

{{Product_GO_help}}

<big>'''Interactions'''</big>


{|class = 'sortable' {{Prettytable}} id='166083'
|- {{table heading style}}
!Partner Type!!Partner!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.J46aa8ad67ea6a&page=24094&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' {{Prettytable}} id='112522'
|- {{table heading style}}
!Compartment!!Description!!Evidence!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.N46a81e0a21360&page=24094&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|{{Prettytable}}
|-
!{{table heading style}} |Sequence
|
[http://biocyc.org/ECOLI/sequence-aa?object=G7071-MONOMER at EcoCyc]
<pre>MNFQQLKIIR EAARQDYNLT EVANMLFTSQ SGVSRHIREL EDELGIEIFV RRGKRLLGMT
EPGKALLVIA ERILNEASNV RRLADLFTND TSGVLTIATT HTQARYSLPE VIKAFRELFP
EVRLELIQGT PQEIATLLQN GEADIGIASE RLSNDPQLVA FPWFRWHHSL LVPHDHPLTQ
ISPLTLESIA KWPLITYRQG ITGRSRIDDA FARKGLLADI VLSAQDSDVI KTYVALGLGI
GLVAEQSSGE QEEENLIRLD TRHLFDANTV WLGLKRGQLQ RNYVWRFLEL CNAGLSVEDI
KRQVMESSEE EIDYQI</pre>
|-
!{{table heading style}} |Mol. Wt
|
35.86 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |pI
|

|-
!{{table heading style}} |Extinction coefficient
|
36440 - 36565 (calc based on 6 Y, 5 W, and 1 C residues)
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.G46aa8ad8633e7&page=24094&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


{|class = 'sortable' {{Prettytable}} id='166085'
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
|-class='sortbottom'
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<big>'''Structure'''</big>


{|class = 'sortable' {{Prettytable}} id='112523'
|- {{table heading style}}
!Structure Type!!Organism!!Accession!!Residues covered!!Notes
|-class='sortbottom'
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</protect>
===Notes===

==Gene Product Resources==
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{|class = 'sortable' {{Prettytable}} id='166086'
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!Resource type!!Source!!Notes/Reference
|-class='sortbottom'
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<protect></protect>
===Notes===

==Accessions in Other Databases==
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{|class = 'sortable' {{Prettytable}} id='166087'
|- {{table_heading_style}}
!Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G7071-MONOMER EcoCyc:G7071-MONOMER]
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|-
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[http://swissmodel.expasy.org/repository/ SwissModel] ([[SwissModel|EcoliWiki Page]])
|
[http://swissmodel.expasy.org/repository/smr.php?job=2&sptr_ac=Q47083 SwissModel:Q47083]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[http://www.expasy.ch/cgi-bin/sprot-search-ac?Q47083 UniProt:Q47083]
|

|-
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[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF00126 PFAM:PF00126]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_416492 RefSeq:NP_416492]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=Q47083 ModBase:Q47083]
|

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===Notes===

==Links==
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{|class = 'sortable' {{Prettytable}} id='112524'
|- {{table_heading_style}}
!Name!!URL!!Comments
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.24094.F46a81e104b863&page=24094&pagename={{FULLPAGENAMEE}} edit table] || ||
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<noinclude>
==References==
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{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0006790_!_sulfur metabolic process]][[Category:GO:0006350_!_transcription]][[Category:GO:0019344_!_cysteine biosynthetic process]][[Category:GO:0016564_!_transcription repressor activity]][[Category:GO:0005737_!_cytoplasm]][[Category:GO:0016563_!_transcription activator activity]]