EcoliWiki - User contributions [en]

SAR endolysin

2011-10-05T20:46:13Z

Gkuty: /* The SAR Paradigm */

== The SAR Paradigm ==
Signal Anchor Release (SAR) endolysins muralytic enzymes encoded by dsDNA phages that infect gram negative hosts. They are signaled to the host cytoplasmic membrane and are anchored there in an inactive conformation. They are maintained in the energized membrane and are released by the depolarization action of the holin. After release, the SAR domain folds into the soluble form of the enzyme, activating it.

The first SAR endolysin to be experimentally characterized was P1 from Enterobacteria phage P1. Cells expressing plasmid borne Lyz lyse independently of a holin suggesting that the protein is secreted to the periplasm. Indeed, Lyz mediated lysis is prevented by the addition of azide, a SecA inhibitor. A sub-cellular fractionation of cells expressing Lyz showed that the protein was partitioned both into the membrane and soluble fractions of the cell. The apparent molecular masses, however, of soluble and membrane bound Lyz were the same, indicating that the secreted protein is not the result of signal peptide cleavage. Taken together, the data indicate that the 20-30 amino acid N-terminal domain is responsible for: a) secretion of the protein through the Sec pathway, b) tethering the protein to the cytoplasmic membrane, and c) releasing the protein into the periplasm.

== Regulation ==
====Disulfide Bond Isomerization====

====Regulation by Dynamic Membrane Topology====

== Prevalence ==
== Role in Pathogenesis ==

== See also ==

==References==
{{RefHelp}}
<references/>

__NOTOC__

SAR endolysin

2011-10-05T20:45:43Z

Gkuty: /* The SAR Paradigm */

== The SAR Paradigm ==
Signal Anchor Release (SAR) endolysins muralytic enzymes encoded by dsDNA phages that infect gram negative hosts. They are signaled to the host cytoplasmic membrane and are anchored there in an inactive conformation. They are maintained in the energized membrane and are released by the depolarization action of the holin. After release, the SAR domain folds into the soluble form of the enzyme, activating it.
The first SAR endolysin to be experimentally characterized was P1 from Enterobacteria phage P1. Cells expressing plasmid borne Lyz lyse independently of a holin suggesting that the protein is secreted to the periplasm. Indeed, Lyz mediated lysis is prevented by the addition of azide, a SecA inhibitor. A sub-cellular fractionation of cells expressing Lyz showed that the protein was partitioned both into the membrane and soluble fractions of the cell. The apparent molecular masses, however, of soluble and membrane bound Lyz were the same, indicating that the secreted protein is not the result of signal peptide cleavage. Taken together, the data indicate that the 20-30 amino acid N-terminal domain is responsible for: a) secretion of the protein through the Sec pathway, b) tethering the protein to the cytoplasmic membrane, and c) releasing the protein into the periplasm.

== Regulation ==
====Disulfide Bond Isomerization====

====Regulation by Dynamic Membrane Topology====

== Prevalence ==
== Role in Pathogenesis ==

== See also ==

==References==
{{RefHelp}}
<references/>

__NOTOC__

SAR endolysin

2011-10-05T20:36:32Z

Gkuty: /* Disulfide Bond Isomerization */

SAR endolysin

2011-10-05T20:29:24Z

Gkuty: /* The SAR Paradigm */

SAR endolysin

2011-10-03T00:33:58Z

Gkuty:

== The SAR Paradigm ==

== Regulation ==
====Disulfide Bond Isomerization====
====Regulation by Dynamic Membrane Topology====

== Prevalence ==
== Role in Pathogenesis ==

== See also ==

==References==
{{RefHelp}}
<references/>

__NOTOC__

SAR endolysin

2011-10-03T00:33:39Z

Gkuty: /* Prevalence */

Bacteriophage M13

== The SAR Paradigm ==

== Regulation ==
====Disulfide Bond Isomerization====
====Regulation by Dynamic Membrane Topology====

== Prevalence ==
== Role in Pathogenesis ==

== See also ==

==References==
{{RefHelp}}
<references/>

__NOTOC__

SAR endolysin

2011-10-03T00:32:59Z

Gkuty: /* See also */

Bacteriophage M13

== The SAR Paradigm ==

== Regulation ==
====Disulfide Bond Isomerization====
====Regulation by Dynamic Membrane Topology====

== Prevalence ==

== See also ==

==References==
{{RefHelp}}
<references/>

__NOTOC__

SAR endolysin

2011-10-03T00:32:46Z

Gkuty: /* Life cycle */

Bacteriophage M13

== The SAR Paradigm ==

== Regulation ==
====Disulfide Bond Isomerization====
====Regulation by Dynamic Membrane Topology====

== Prevalence ==

== See also ==
[http://en.wikipedia.org/wiki/M13_bacteriophage M13 bacteriophage] on Wikipedia.

==References==
{{RefHelp}}
<references/>

__NOTOC__

SAR endolysin

2011-10-03T00:32:24Z

Gkuty: /* Regulation */

Bacteriophage M13

== The SAR Paradigm ==

== Regulation ==
====Disulfide Bond Isomerization====
====Regulation by Dynamic Membrane Topology====

== Life cycle ==

==== Adsorption and Injection ====

==== DNA replication ====

==== Packaging ====

==== Lysis ====

== See also ==
[http://en.wikipedia.org/wiki/M13_bacteriophage M13 bacteriophage] on Wikipedia.

==References==
{{RefHelp}}
<references/>

__NOTOC__

SAR endolysin

2011-10-03T00:31:34Z

Gkuty: /* Regulation */

Bacteriophage M13

== The SAR Paradigm ==

== Regulation ==
====Disulfide Bond Isomerization====

== Life cycle ==

==== Adsorption and Injection ====

==== DNA replication ====

==== Packaging ====

==== Lysis ====

== See also ==
[http://en.wikipedia.org/wiki/M13_bacteriophage M13 bacteriophage] on Wikipedia.

==References==
{{RefHelp}}
<references/>

__NOTOC__

SAR endolysin

2011-10-03T00:30:49Z

Gkuty: /* Genome Organization */

Bacteriophage M13

== The SAR Paradigm ==

== Regulation ==

== Life cycle ==

==== Adsorption and Injection ====

==== DNA replication ====

==== Packaging ====

==== Lysis ====

== See also ==
[http://en.wikipedia.org/wiki/M13_bacteriophage M13 bacteriophage] on Wikipedia.

==References==
{{RefHelp}}
<references/>

__NOTOC__

SAR endolysin

2011-10-03T00:30:14Z

Gkuty: /* lodkdirehjeber */

Bacteriophage M13

== The SAR Paradigm ==

== Genome Organization ==
ssDNA genome

Genome map of f1 described by Lyons & Zinder (1972). <ref name='PMID:4556929'/>

== Life cycle ==

==== Adsorption and Injection ====

==== DNA replication ====

==== Packaging ====

==== Lysis ====

== See also ==
[http://en.wikipedia.org/wiki/M13_bacteriophage M13 bacteriophage] on Wikipedia.

==References==
{{RefHelp}}
<references/>

__NOTOC__

SAR endolysin

2011-08-02T20:10:49Z

Gkuty: New Template:Empty_Page Page

==References==
{{RefHelp}}
<references/>

__NOTOC__

secB:Quickview

2007-11-20T22:32:23Z

Gkuty: edited by Gkuty via TableEdit

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{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
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{|{{Prettytable}}
|-
!{{table_heading_style}} |Gene Name
|
[[secB:Gene|''secB]]'' (synonyms: ''ECK3599, b3609, JW3584'')<ref name='LIB:Riley_2006'/>
|-
!{{table_heading_style}} |Product(s)
|
[[secB:Gene_Product(s)|SecB product]]<ref name='LIB:Riley_2006'/>: protein export chaperone product, B3609 product
|-
!{{table_heading_style}} |Description
|
SecB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

Component of [[:Category:Complex:Sec Protein Secretion Complex|Sec Protein Secretion Complex]]<ref name='LIB:EcoCyc10.6'/>

|-
!{{table_heading_style}} |Knock-Out Phenotype
|
non lethal. SecB null strains are deficient in post translational targeting of some secretion and outer membrane proteins.<ref name=PMID:3891730/>
|-
!{{table_heading_style}} |Regulation/Expression
|
transcription unit(s): [[:Category:Txn_unit:gpsA|gpsA]]<ref name='LIB:EcoCyc10.6'/>
|-
!{{table_heading_style}} |Regulation/Activity
|

|-
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===Notes===
{{BacktoTop}}

<noinclude>
==References==
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{{RefHelp}}
<references/>
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secB:Quickview

2007-11-20T22:27:35Z

Gkuty: edited by Gkuty via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab On|Quickview|secB}}{{Tab|Gene|secB}}{{Tab|Gene Product(s)|secB}}{{Tab|Expression|secB}}{{Tab|Evolution|secB}}{{Tab|On One Page|secB}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>


{|{{Prettytable}}
|-
!{{table_heading_style}} |Gene Name
|
[[secB:Gene|''secB]]'' (synonyms: ''ECK3599, b3609, JW3584'')<ref name='LIB:Riley_2006'/>
|-
!{{table_heading_style}} |Product(s)
|
[[secB:Gene_Product(s)|SecB product]]<ref name='LIB:Riley_2006'/>: protein export chaperone product, B3609 product
|-
!{{table_heading_style}} |Description
|
SecB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

Component of [[:Category:Complex:Sec Protein Secretion Complex|Sec Protein Secretion Complex]]<ref name='LIB:EcoCyc10.6'/>

|-
!{{table_heading_style}} |Knock-Out Phenotype
|
non lethal. SecB null strains are deficient in post translational targeting of some secretion and outer membrane proteins.
|-
!{{table_heading_style}} |Regulation/Expression
|
transcription unit(s): [[:Category:Txn_unit:gpsA|gpsA]]<ref name='LIB:EcoCyc10.6'/>
|-
!{{table_heading_style}} |Regulation/Activity
|

|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.8960.Y46a9a208d4254&page=8960&pagename={{FULLPAGENAMEE}} edit table]
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{{Quickview_table_help}}
<protect></protect>
===Notes===
{{BacktoTop}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
</noinclude>

secB:Quickview

2007-11-20T22:25:32Z

Gkuty: edited by Gkuty via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab On|Quickview|secB}}{{Tab|Gene|secB}}{{Tab|Gene Product(s)|secB}}{{Tab|Expression|secB}}{{Tab|Evolution|secB}}{{Tab|On One Page|secB}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>


{|{{Prettytable}}
|-
!{{table_heading_style}} |Gene Name
|
[[secB:Gene|''secB]]'' (synonyms: ''ECK3599, b3609, JW3584'')<ref name='LIB:Riley_2006'/>
|-
!{{table_heading_style}} |Product(s)
|
[[secB:Gene_Product(s)|SecB product]]<ref name='LIB:Riley_2006'/>: protein export chaperone product, B3609 product
|-
!{{table_heading_style}} |Description
|
SecB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

Component of [[:Category:Complex:Sec Protein Secretion Complex|Sec Protein Secretion Complex]]<ref name='LIB:EcoCyc10.6'/>

|-
!{{table_heading_style}} |Knock-Out Phenotype
|
non lethal. SecB null strains are deficient in post translational targeting of some secretion and outer membrane proteins. Ref: Kumamoto and Beckwith. J. Bacteriol. (1985) 163: 267-274.
|-
!{{table_heading_style}} |Regulation/Expression
|
transcription unit(s): [[:Category:Txn_unit:gpsA|gpsA]]<ref name='LIB:EcoCyc10.6'/>
|-
!{{table_heading_style}} |Regulation/Activity
|

|-
|colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.8960.Y46a9a208d4254&page=8960&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Quickview_table_help}}
<protect></protect>
===Notes===
{{BacktoTop}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
</noinclude>