EcoliWiki - User contributions [en]

glnA:Gene Product(s)

2008-12-20T21:23:40Z

Dkornfi1: /* Notes */

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<noinclude>{{TabsTop}}
{{Tab|Quickview|glnA}}{{Tab|Gene|glnA}}{{Tab On|Gene Product(s)|glnA}}{{Tab|Expression|glnA}}{{Tab|Evolution|glnA}}{{Tab|On One Page|glnA}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


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|-
!align=left {{table heading style}} |Standard name
||
GlnA
|-
!align=left {{table heading style}} |Synonyms
||
glutamine synthetase<ref name='LIB:Riley_2006'/>, B3870<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, adenylyl-[L-glutamate:ammonia Ligase (ADP-forming)]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, adenylyl-GS<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
adenylyl-[glutamine synthetase]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>, GlnA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;

Component of ; [[:Category:Complex:glutamine synthetase|glutamine synthetase]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

|-
!align=left {{table heading style}} |EC number (for enzymes)
||
** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2]<ref name='LIB:Riley_2006'/>

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===Notes===

==Function==
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<protect>
<big>'''Gene Ontology'''</big>



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|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0004356_!_glutamate-ammonia_ligase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0004356_!_glutamate-ammonia_ligase_activity GO:0004356]
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glutamate-ammonia ligase activity
|
[[PMID:6102984]]<ref name=PMID:6102984/>
|
IDA: Inferred from Direct Assay
|

|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0005624_!_membrane_fraction]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005624_!_membrane_fraction GO:0005624]
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membrane fraction
|
[[PMID:16858726]]<ref name=PMID:16858726/>
|
IDA: Inferred from Direct Assay
|

|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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complete
|-
|

|
[[Category:GO:0019676_!_ammonia_assimilation_cycle]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0019676_!_ammonia_assimilation_cycle GO:0019676]
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ammonia assimilation cycle
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[[PMID:6102984]]<ref name=PMID:6102984/>
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IDA: Inferred from Direct Assay
|

|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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complete
|-
|

|
[[Category:GO:0019740_!_nitrogen_utilization]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0019740_!_nitrogen_utilization GO:0019740]
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nitrogen utilization
|
[[PMID:6102984]]<ref name=PMID:6102984/>
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IDA: Inferred from Direct Assay
|

|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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complete
|-
|

|
[[Category:GO:0000166_!_nucleotide_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0000166_!_nucleotide_binding GO:0000166]
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nucleotide binding
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[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
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IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0547 SP_KW:KW-0547]
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F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0004356_!_glutamate-ammonia_ligase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0004356_!_glutamate-ammonia_ligase_activity GO:0004356]
|
glutamate-ammonia ligase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000003 GO_REF:0000003]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR001637 InterPro:IPR001637] [http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR004809 InterPro:IPR004809] [http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR008146 InterPro:IPR008146] [http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR008147 InterPro:IPR008147] EC:6.3.1.2
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F
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Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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complete
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR004809 InterPro:IPR004809] [http://www.uniprot.org/keywords/KW-0963 SP_KW:KW-0963]
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C
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Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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complete
|-
|

|
[[Category:GO:0006542_!_glutamine_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006542_!_glutamine_biosynthetic_process GO:0006542]
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glutamine biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR008147 InterPro:IPR008147]
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P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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complete
|-
|

|
[[Category:GO:0009399_!_nitrogen_fixation]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009399_!_nitrogen_fixation GO:0009399]
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nitrogen fixation
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[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR004809 InterPro:IPR004809]
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P
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Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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complete

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<big>'''Interactions'''</big>


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!|Partner Type!!Partner!!Notes!!References
|-
|
Protein
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Subunits of [[:Category:Complex:glutamine synthetase|glutamine synthetase]]
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could be indirect
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</protect>

===Notes===
Glutamine Synthetase GlnA regulates cytoplasmic nitrogen levels. It catalyzes the reaction between glutamine and oxoglutarate to form 2 molecules of glutamate. The reaction is energetically expensive and consumes one ATP per reaction. The enzyme catalyzes the final step of ammonia fixation. Glutamine synthetase is thought to interact with PII proteins and regulates ammonia fixation rate in other bacteria.

==Localization==


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!|Compartment!!Description!!Evidence!!Notes
|-
|
cytoplasm
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|

|
From EcoCyc<ref name='LIB:EcoCyc11.1'/>

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===Notes===

==Structure and Physical Properties==
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<protect>
<big>'''Physical Properties'''</big>


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|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=ADENYLYL-GS at EcoCyc]
<pre>MSAEHVLTML NEHEVKFVDL RFTDTKGKEQ HVTIPAHQVN AEFFEEGKMF DGSSIGGWKG
INESDMVLMP DASTAVIDPF FADSTLIIRC DILEPGTLQG YDRDPRSIAK RAEDYLRSTG
IADTVLFGPE PEFFLFDDIR FGSSISGSHV AIDDIEGAWN SSTQYEGGNK GHRPAVKGGY
FPVPPVDSAQ DIRSEMCLVM EQMGLVVEAH HHEVATAGQN EVATRFNTMT KKADEIQIYK
YVVHNVAHRF GKTATFMPKP MFGDNGSGMH CHMSLSKNGV NLFAGDKYAG LSEQALYYIG
GVIKHAKAIN ALANPTTNSY KRLVPGYEAP VMLAYSARNR SASIRIPVVS SPKARRIEVR
FPDPAANPYL CFAALLMAGL DGIKNKIHPG EAMDKNLYDL PPEEAKEIPQ VAGSLEEALN
ELDLDREFLK AGGVFTDEAI DAYIALRREE DDRVRMTPHP VEFELYYSV</pre>
|-
!align=left {{table heading style}} |Mol. Wt
||
51.9 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!align=left {{table heading style}} |pI
||
5.47
|-
!align=left {{table heading style}} |Extinction coefficient
||
36330 - 36830 (calc based on 17 Y, 2 W, and 4 C residues)

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<big>'''Domains/Motifs/Modification Sites'''</big>


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|- {{table heading style}}
!|Type!!Residues!!Description!!Notes
|-
|
Modification Site
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398
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O-AMP-tyrosine
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[http://www.uniprot.org/uniprot/P0A9C5#section_features UniProt:P0A9C5]

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<big>'''Structure'''</big>


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|-
!align=left {{table heading style}} |Structures
||
<beststructure>glnA
562</beststructure>

|-
!align=left {{table heading style}} |Models
||

View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=glnA&sub=keywordSearc EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=glnA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

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===Notes===

==Gene Product Resources==
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!|Resource type!!Source!!Notes/Reference

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===Notes===

==Accessions in Other Databases==
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!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=ADENYLYL-GS EcoCyc:ADENYLYL-GS]
|

|-
|
[http://swissmodel.expasy.org/repository/ SwissModel] ([[SwissModel|EcoliWiki Page]])
|
[http://swissmodel.expasy.org/repository/smr.php?job=2&sptr_ac=P0A9C5 SwissModel:P0A9C5]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P0A9C5|UniProt:P0A9C5]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF03951 PFAM:PF03951]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_418306 RefSeq:NP_418306]
|

|-
|
[http://www.rcsb.org/pdb PDB] ([[PDB|EcoliWiki Page]])
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[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2GLS PDB:2GLS]
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===Notes===

==Links==
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!|Name!!URL!!Comments

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<noinclude>
==References==
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{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0006542_!_glutamine biosynthetic process]][[Category:GO:0006807_!_nitrogen compound metabolic process]][[Category:Products with structures in PDB]][[Category:Complex:glutamine synthetase]][[Category:Location:cytoplasm]]

urea cycle

2008-12-18T07:51:37Z

Dkornfi1: New page: The Urea Cycle in E. coli is an important pathway to store excess nitrogen. Mitochondrial nitrogen reacts with solubilized bicarbonate to form carbomyl phosphate. One molecule of ATP is co...

The Urea Cycle in E. coli is an important pathway to store excess nitrogen. Mitochondrial nitrogen reacts with solubilized bicarbonate to form carbomyl phosphate. One molecule of ATP is consumed to supply the phosphate group for carbomyl phosphate molecule. Another ATP molecule is consumed to supply the energy for this reaction. Carbamoyl phosphate reacts with ornithine to generate citrulline. An aspartate molecule adds to create arginosuccinate. ATP is cleaved to AMP and pyrophosphate to drive this reaction forward. Fumarate is cleaved off of arginosuccinate. Fumarate can then be used for the ATP cycle. Arginine is the other product of this reaction and can be used for protein synthesis. In other bacteria, hydrolysis of arginine generates ornithine and urea. Urea is then excreted. However, E. coli lacks the enzyme to do this hydrolysis and therefore this cycle is terminated by arginine synthesis.

uhpA:Expression

2008-09-18T19:34:34Z

Dkornfi1: /* Categories */

uhpA:Expression

2008-09-18T19:34:20Z

Dkornfi1: /* Categories */

uhpT:Expression

2008-09-18T19:31:52Z

Dkornfi1: /* Categories */

Category:Regulon:UhpAB

2008-09-18T19:31:02Z

Dkornfi1: Removing all content from page

uhpB:Expression

2008-09-18T19:30:37Z

Dkornfi1: /* Categories */

uhpA:Gene Product(s)

2008-09-18T19:29:26Z

Dkornfi1: /* Categories */

Category:Regulon:UhpAB

2008-09-18T19:26:43Z

Dkornfi1: New page: uhpB:On One Page

[[uhpB:On One Page]]

uhpA:Gene Product(s)

2008-09-18T19:25:48Z

Dkornfi1: /* Categories */

uhpA:Gene Product(s)

2008-09-18T19:20:31Z

Dkornfi1: /* Notes */

uhpA:Evolution

2008-09-18T19:02:58Z

Dkornfi1: Table edited by Dkornfi1 via TableEdit