EcoliWiki - User contributions [en]

PMID:11830595

2008-11-25T16:59:03Z

D-struck: Table edited by D-struck via TableEdit

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 '''Vorachek-Warren, MK, Ramirez, S, Cotter, RJ and Raetz, CR''' (2002) A triple mutant of Escherichia coli lacking secondary acyl chains on lipid A.''J. Biol. Chem.'' '''277''':14194-205

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|{{TableHeadingColor}} |'''Abstract''' || All possible combinations of insertion mutations in the three genes encoding the acyl carrier protein-dependent late acyltransferases of lipid A biosynthesis, designated lpxL(htrB), lpxM(msbB), and lpxP, were generated in Escherichia coli K12 W3110. Mutants defective in lpxM synthesize penta-acylated lipid A molecules and grow normally. Strains lacking lpxP fail to incorporate palmitoleate into their lipid A at 12 degrees C but make normal amounts of hexa-acylated lipid A and are viable. Although lpxL mutants and lpxL lpxM double mutants grow slowly on minimal medium at all temperatures, they do not grow on nutrient broth above 32 degrees C. Such mutants retain the ability to synthesize some penta- and hexa-acylated lipid A molecules because of limited induction of lpxP at 30 degrees C but not above 32 degrees C. MKV15, an E. coli lpxL lpxM lpxP triple mutant, likewise grows slowly on minimal medium at all temperatures but not on nutrient broth at any temperature. MKV15 synthesizes a lipid A molecule containing only the four primary (R)-3-hydroxymyristoyl chains. The outer membrane localization and content of lipid A are nearly normal in MKV15, as is the glycerophospholipid and membrane protein composition. However, the rate at which the tetra-acylated lipid A of MKV15 is exported to the outer membrane is reduced compared with wild type. The integrity of the outer membrane of MKV15 is compromised, as judged by antibiotic hypersensitivity, and MKV15 undergoes lysis following centrifugation. MKV15 may prove useful as a host strain for expressing late acyltransferase genes from other Gram-negative bacteria, facilitating the re-engineering of lipid A structure in living cells and the design of novel vaccines.
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11830595 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxL
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
IMP: Inferred from Mutant Phenotype
|

|
P
|
Kdo2-[4’-32P]-tetraacyldisaccharide-1,4’ bisphosphate (Kdo2-[4’-32P]-lipid IVA) accumulates in a lpxLlpxMlpxP triple mutant; penta- and hexa- acylated derivatives are not formed (see Fig.4 for ms analysis and Fig. 3 for corresponding structures).
|
complete

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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

PMID:8662613

2008-11-25T16:57:25Z

D-struck: Table edited by D-struck via TableEdit

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 '''Clementz, T, Bednarski, JJ and Raetz, CR''' (1996) Function of the htrB high temperature requirement gene of Escherchia coli in the acylation of lipid A: HtrB catalyzed incorporation of laurate.''J. Biol. Chem.'' '''271''':12095-102

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|{{TableHeadingColor}} |'''Abstract''' || By assaying lysates of Escherichia coli generated with the hybrid lambda bacteriophages of an ordered library (Kohara, Y., Akiyama, K., and Isono, K. (1987) Cell 50, 495-508), we identified two clones (lambda232 and lambda233) capable of overexpressing the lauroyl transferase that functions after 3-deoxy-D-manno-octulosonic acid (Kdo) addition in lipid A biosynthesis (Brozek, K. A., and Raetz, C. R. H. (1990) J. Biol. Chem. 265, 15410-15417). The E. coli DNA inserts in lambda232 and lambda233 suggested that a known gene (htrB) required for rapid growth above 33 degrees C might encode the lauroyl transferase. Using the intermediate (Kdo)2-lipid IVA as the laurate acceptor, extracts of strains with transposon insertions in htrB were found to contain no lauroyl transferase activity. Cells harboring hybrid htrB+ plasmids overproduced transferase activity 100-200-fold. The overproduced transferase was solubilized with a non-ionic detergent and purified further by DEAE-Sepharose chromatography. With lauroyl acyl carrier protein as the donor, the purified enzyme rapidly incorporated one laurate residue into (Kdo)2-lipid IVA. The rate of laurate incorporation was reduced by several orders of magnitude when either one or both Kdos were absent in the acceptor. With a matched set of acyl-acyl carrier proteins, the enzyme incorporated laurate 3-8 times faster than decanoate or myristate, respectively. Transfer of palmitate, palmitoleate, or R-3-hydroxymyristate was very slow. Taken together with previous studies, our findings indicate that htrB encodes a key, late functioning acyltransferase of lipid A biosynthesis.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8662613 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxL
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
LpxL is assayed using lauryl-ACP and Kdo2-[4’-32P]-tetraacyldisaccharide-1,4’ bisphosphate (Kdo2-[4’-32P]-lipid IVA) as substrates. The extent of the reaction is determined using a PhosphorImager after separation of the product from the substrates by thin layer chromatography (pg. 12096 and Figs. 2 and 3). The lauryl group is esterified to the hydroxyl group of the N-linked hydroxy-myristate of the distal glucosamine residue of Kdo2-[4’-32P]-lipid IVA (see Fig. 1). LpxL activity is not detected in strains with a lpxL::Tn10 insertion (Fig. 3 and Table II). The substrates are not commercially available but their preparation is described.
|
complete
|-
|
LpxL
|

|
[[Category:GO:0016020_!_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016020_!_membrane GO:0016020]
|
membrane
|
IDA: Inferred from Direct Assay
|

|
C
|
LpxL is purified from membrane fractions obtained after disruption of overproducing strains by passage through a French pressure cell. Membranes were separated from the soluble fraction by centrifugation at 150,000 x g for 60 minutes (pg. 12096 and Table III).
|
complete

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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

lpxL:Gene Product(s)

2008-11-25T16:54:54Z

D-struck: Table edited by D-struck via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|lpxL}}{{Tab|Gene|lpxL}}{{Tab On|Gene Product(s)|lpxL}}{{Tab|Expression|lpxL}}{{Tab|Evolution|lpxL}}{{Tab|On One Page|lpxL}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Standard name
||
LpxL product
|-
!align=left {{table heading style}} |Synonyms
||
lauryl-acyl carrier protein (ACP)-dependent acyltransferase product<ref name='LIB:Riley_2006'/>, B1054 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, WaaM product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, HtrB product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, LpxL product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
LAUROYLACYLTRAN-MONOMER<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

|-
!align=left {{table heading style}} |EC number (for enzymes)
||
** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.- 2.3.1.-]<ref name='LIB:Riley_2006'/>

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{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> {{Product_GO_help}}

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0016747_!_transferase_activity,_transferring_acyl_groups_other_than_amino-acyl_groups]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016747_!_transferase_activity,_transferring_acyl_groups_other_than_amino-acyl_groups GO:0016747]
|
transferase activity, transferring groups other than amino-acyl groups
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR011920 InterPro:IPR011920]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0808 SP_KW:KW-0808]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016021_!_integral_to_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016021_!_integral_to_membrane GO:0016021]
|
integral to membrane
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR004960 InterPro:IPR004960] [http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR011920 InterPro:IPR011920] [http://www.uniprot.org/keywords/KW-0812 SP_KW:KW-0812]
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016020_!_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016020_!_membrane GO:0016020]
|
membrane
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0397 SP_KW:KW-0397] [http://www.uniprot.org/keywords/KW-0472 SP_KW:KW-0472]
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009276_!_Gram-negative-bacterium-type_cell_wall]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009276_!_Gram-negative-bacterium-type_cell_wall GO:0009276]
|
Gram-negative-bacterium-type cell wall
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR011920 InterPro:IPR011920]
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009244_!_lipopolysaccharide_core_region_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009244_!_lipopolysaccharide_core_region_biosynthetic_process GO:0009244]
|
lipopolysaccharide core region biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR004960 InterPro:IPR004960]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009103_!_lipopolysaccharide_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009103_!_lipopolysaccharide_biosynthetic_process GO:0009103]
|
lipopolysaccharide biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0448 SP_KW:KW-0448]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008415_!_acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008415_!_acyltransferase_activity GO:0008415]
|
acyltransferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR004960 InterPro:IPR004960] [http://www.uniprot.org/keywords/KW-0012 SP_KW:KW-0012]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0006950_!_response_to_stress]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006950_!_response_to_stress GO:0006950]
|
response to stress
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0346 SP_KW:KW-0346]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0031225_!_anchored_to_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0031225_!_anchored_to_membrane GO:0031225]
|
anchored to membrane
|

|

|

|
C
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|

|

|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
[[PMID:8662613]]<ref name=PMID:8662613/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete
|-
|

|
[[Category:GO:0016020_!_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016020_!_membrane GO:0016020]
|
membrane
|
[[PMID:8662613]]<ref name=PMID:8662613/>
|
IDA: Inferred from Direct Assay
|

|
C
|

|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[[PMID:11830595]]<ref name=PMID:11830595/>
|
IMP: Inferred from Mutant Phenotype
|

|
P
|

|
complete

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<big>'''Interactions'''</big>


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!|Partner Type!!Partner!!Notes!!References

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{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Notes
|-
|
plasma membrane
|

|

|
From EcoCyc<ref name='LIB:EcoCyc11.1'/>

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{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=LAUROYLACYLTRAN-MONOMER at EcoCyc]
<pre>MTNLPKFSTA LLHPRYWLTW LGIGVLWLVV QLPYPVIYRL GCGLGKLALR FMKRRAKIVH
RNLELCFPEM SEQERRKMVV KNFESVGMGL METGMAWFWP DRRIARWTEV IGMEHIRDVQ
AQKRGILLVG IHFLTLELGA RQFGMQEPGI GVYRPNDNPL IDWLQTWGRL RSNKSMLDRK
DLKGMIKALK KGEVVWYAPD HDYGPRSSVF VPLFAVEQAA TTTGTWMLAR MSGACLVPFV
PRRKPDGKGY QLIMLPPECS PPLDDAETTA AWMNKVVEKC IMMAPEQYMW LHRRFKTRPE
GVPSRY</pre>
|-
!align=left {{table heading style}} |Mol. Wt
||
35.41 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!align=left {{table heading style}} |pI
||

|-
!align=left {{table heading style}} |Extinction coefficient
||
79410 - 80035 (calc based on 9 Y, 12 W, and 5 C residues)

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{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


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|- {{table heading style}}
!|Type!!Residues!!Description!!Notes

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{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>lpxL
562</beststructure>

|-
!align=left {{table heading style}} |Models
||

View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=lpxL&sub=keywordSearc EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=lpxL&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

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{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference

|-class='sortbottom'
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{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=LAUROYLACYLTRAN-MONOMER EcoCyc:LAUROYLACYLTRAN-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P0ACV0|UniProt:P0ACV0]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF03279 PFAM:PF03279]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_415572 RefSeq:NP_415572]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=P0ACV0 ModBase:P0ACV0]
|

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|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.21999.L46a7e5e7cae44&page=21999&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0031225_!_anchored to membrane]][[Category:GO:0009245_!_lipid A biosynthetic process]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]][[Category:Location:plasma membrane]]

lpxL:Gene Product(s)

2008-11-25T16:50:46Z

D-struck: Table edited by D-struck via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|lpxL}}{{Tab|Gene|lpxL}}{{Tab On|Gene Product(s)|lpxL}}{{Tab|Expression|lpxL}}{{Tab|Evolution|lpxL}}{{Tab|On One Page|lpxL}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Standard name
||
LpxL product
|-
!align=left {{table heading style}} |Synonyms
||
lauryl-acyl carrier protein (ACP)-dependent acyltransferase product<ref name='LIB:Riley_2006'/>, B1054 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, WaaM product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, HtrB product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, LpxL product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
LAUROYLACYLTRAN-MONOMER<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

|-
!align=left {{table heading style}} |EC number (for enzymes)
||
** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.- 2.3.1.-]<ref name='LIB:Riley_2006'/>

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|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> {{Product_GO_help}}

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0016747_!_transferase_activity,_transferring_acyl_groups_other_than_amino-acyl_groups]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016747_!_transferase_activity,_transferring_acyl_groups_other_than_amino-acyl_groups GO:0016747]
|
transferase activity, transferring groups other than amino-acyl groups
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR011920 InterPro:IPR011920]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0808 SP_KW:KW-0808]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016021_!_integral_to_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016021_!_integral_to_membrane GO:0016021]
|
integral to membrane
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR004960 InterPro:IPR004960] [http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR011920 InterPro:IPR011920] [http://www.uniprot.org/keywords/KW-0812 SP_KW:KW-0812]
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016020_!_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016020_!_membrane GO:0016020]
|
membrane
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0397 SP_KW:KW-0397] [http://www.uniprot.org/keywords/KW-0472 SP_KW:KW-0472]
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009276_!_Gram-negative-bacterium-type_cell_wall]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009276_!_Gram-negative-bacterium-type_cell_wall GO:0009276]
|
Gram-negative-bacterium-type cell wall
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR011920 InterPro:IPR011920]
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009244_!_lipopolysaccharide_core_region_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009244_!_lipopolysaccharide_core_region_biosynthetic_process GO:0009244]
|
lipopolysaccharide core region biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR004960 InterPro:IPR004960]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009103_!_lipopolysaccharide_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009103_!_lipopolysaccharide_biosynthetic_process GO:0009103]
|
lipopolysaccharide biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0448 SP_KW:KW-0448]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008415_!_acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008415_!_acyltransferase_activity GO:0008415]
|
acyltransferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] [http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR004960 InterPro:IPR004960] [http://www.uniprot.org/keywords/KW-0012 SP_KW:KW-0012]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0006950_!_response_to_stress]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006950_!_response_to_stress GO:0006950]
|
response to stress
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0346 SP_KW:KW-0346]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0031225_!_anchored_to_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0031225_!_anchored_to_membrane GO:0031225]
|
anchored to membrane
|

|

|

|
C
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|

|

|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
[[PMID:86626133]]<ref name=PMID:86626133/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete
|-
|

|
[[Category:GO:0016020_!_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016020_!_membrane GO:0016020]
|
membrane
|
[[PMID:86626133]]<ref name=PMID:86626133/>
|
IDA: Inferred from Direct Assay
|

|
C
|

|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[[PMID:11830595]]<ref name=PMID:11830595/>
|
IMP: Inferred from Mutant Phenotype
|

|
P
|

|
complete

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|}


<big>'''Interactions'''</big>


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Partner Type!!Partner!!Notes!!References

|-class='sortbottom'
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|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Notes
|-
|
plasma membrane
|

|

|
From EcoCyc<ref name='LIB:EcoCyc11.1'/>

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|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=LAUROYLACYLTRAN-MONOMER at EcoCyc]
<pre>MTNLPKFSTA LLHPRYWLTW LGIGVLWLVV QLPYPVIYRL GCGLGKLALR FMKRRAKIVH
RNLELCFPEM SEQERRKMVV KNFESVGMGL METGMAWFWP DRRIARWTEV IGMEHIRDVQ
AQKRGILLVG IHFLTLELGA RQFGMQEPGI GVYRPNDNPL IDWLQTWGRL RSNKSMLDRK
DLKGMIKALK KGEVVWYAPD HDYGPRSSVF VPLFAVEQAA TTTGTWMLAR MSGACLVPFV
PRRKPDGKGY QLIMLPPECS PPLDDAETTA AWMNKVVEKC IMMAPEQYMW LHRRFKTRPE
GVPSRY</pre>
|-
!align=left {{table heading style}} |Mol. Wt
||
35.41 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!align=left {{table heading style}} |pI
||

|-
!align=left {{table heading style}} |Extinction coefficient
||
79410 - 80035 (calc based on 9 Y, 12 W, and 5 C residues)

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|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


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|- {{table heading style}}
!|Type!!Residues!!Description!!Notes

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|}

{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>lpxL
562</beststructure>

|-
!align=left {{table heading style}} |Models
||

View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=lpxL&sub=keywordSearc EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=lpxL&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

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{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference

|-class='sortbottom'
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|}

{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=LAUROYLACYLTRAN-MONOMER EcoCyc:LAUROYLACYLTRAN-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P0ACV0|UniProt:P0ACV0]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF03279 PFAM:PF03279]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_415572 RefSeq:NP_415572]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=P0ACV0 ModBase:P0ACV0]
|

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|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|-class='sortbottom'
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|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0031225_!_anchored to membrane]][[Category:GO:0009245_!_lipid A biosynthetic process]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]][[Category:Location:plasma membrane]]

lpxL:Quickview

2008-11-25T16:47:16Z

D-struck: Table edited by D-struck via TableEdit

PMID:1577828

2008-11-10T16:51:28Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Belunis, CJ and Raetz, CR''' (1992) Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli.''J. Biol. Chem.'' '''267''':9988-97

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || The enzyme 3-deoxy-D-manno-octulosonic acid (Kdo) transferase is encoded by the kdtA gene of Escherichia coli and plays a key role in lipopolysaccharide biosynthesis. It transfers Kdo from CMP-Kdo to lipid A or its tetraacyldisaccharide-1,4'-bisphosphate precursor, lipid IVA. Using a strain that overproduces the transferase approximately 500-fold, we have purified the enzyme to near homogeneity. The subunit molecular mass is approximately 43 kDa. Activity is stimulated by Triton X-100, is maximal at pH 7, but does not require Mg2+. The apparent Km values for lipid IVA and CMP-Kdo are 52 and 88 microM, respectively. Vmax is 15-18 mumol/min/mg when both substrates are added near saturation at pH 8. The purified enzyme transfers 2 Kdo residues to lipid A precursors or analogs bearing four to six fatty acyl chains and a 4'-monosphosphate moiety. Activity is inhibited by polymixin B and Re endotoxin. At low Kdo concentrations small amounts of the intermediate, (Kdo)1-IVA, accumulate. When this substance is isolated and incubated with purified enzyme in the presence of CMP-Kdo, it is converted to (Kdo)2-IVA. Formation of (Kdo)1-IVA is also observed when purified enzyme is incubated with (Kdo)2-IVA and 5 mM CMP, demonstrating that Kdo transfer is reversible. In summary, Kdo transferase consists of a single bifunctional polypeptide that incorporates the 2 innermost Kdo residues common to all lipopolysaccharide molecules in E. coli.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1577828 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
WaaA
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
In the normal assay for Kdo transferase activity, CMP-Kdo is generated in situ using CMP-Kdo synthase, Kdo and CTP. At limiting Kdo concentrations, the intermediate Kdo1-lipid IVA accumulates (Fig. 6). Kdo1-lipid IVA can be converted into Kdo2-lipid IVA by the WaaA enzyme in the presence of a CMP-Kdo generating system supplied with higher Kdo concentrations (Fig. 7).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.C47aab4d0a3d7b&page=127984&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

PMID:7499229

2008-11-10T16:45:49Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Belunis, CJ, Clementz, T, Carty, SM and Raetz, CR''' (1995) Inhibition of lipopolysaccharide biosynthesis and cell growth following inactivation of the kdtA gene in Escherichia coli.''J. Biol. Chem.'' '''270''':27646-52

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || The enzyme 3-deoxy-D-manno-octulosonic acid (Kdo) transferase is encoded by the kdtA gene in Escherichia coli. The enzyme is a single polypeptide that catalyzes the transfer of two Kdo residues to a tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A, designated lipid IVA (Belunis, C.J., and Raetz, C.R.H. (1992) J. Biol. Chem. 267, 9988-9997). To determine if Kdo transfer to lipid IVA is required for growth, we constructed a strain of E. coli with a chromosomal kdtA::kan insertion mutation. In mutants carrying the kdtA::kan allele on the chromosome, cell growth and Kdo transferase activity were dependent upon a copy of the intact kdtA gene on a plasmid. When the kdtA-bearing plasmid was itself temperature sensitive for replication, the growth of these strains was inhibited after several hours at 44 degrees C, and Kdo transferase activity in extracts became undetectable. Concomitantly, the cells accumulated massive amounts of lipid IVA, the precursor of (Kdo)2-lipid IVA. The kdtA::kan mutation could also be complemented by hybrid plasmids bearing the gseA gene of Chlamydia trachomatis. gseA specifies a distinct Kdo transferase that adds three Kdo moieties to lipid IVA. Lipopolysaccharide from E. coli kdtA::kan constructs complemented by gseA reacts strongly with antibodies directed against the genus-specific epitope of Chlamydia, whereas lipopolysaccharide from parental E. coli K-12 does not. Our studies prove that Kdo attachment during lipid A biosynthesis is essential for cell growth and accounts for the conditional lethality associated with mutations in Kdo biosynthesis.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7499229 PubMed]
[http://www.ncbi.nlm.nih.gov/entrez/utils/fref.fcgi?PrId=3051&itool=AbstractPlus-def&uid=7499229&db=pubmed&url=http://www.jbc.org/cgi/pmidlookup?view=long&pmid=7499229 Full text]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
WaaA
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
IMP: Inferred from Mutant Phenotype
|

|
P
|
The construction of a strain with a kanR insertion in the WaaA gene and complemented by a plasmid that is temperature sensitive for replication and carrying WaaA gene is described. At elevated temperature, this strain is non-viable and accumulates lipid IVA (Fig. 6).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.N47aa917825889&page=125846&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

PMID:2651435

2008-11-10T16:43:56Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Brozek, KA, Hosaka, K, Robertson, AD and Raetz, CR''' (1989) Biosynthesis of lipopolysaccharide in Escherichia coli. Cytoplasmic enzymes that attach 3-deoxy-D-manno-octulosonic acid to lipid A.''J. Biol. Chem.'' '''264''':6956-66

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || Previous studies in our laboratory led to the elucidation of the covalent structure of a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (designated lipid IVA), that accumulates at 42 degrees C in temperature-sensitive mutants defective in 3-deoxy-D-manno-octulosonic acid (KDO) biosynthesis (Raetz, C. R. H., Purcell, S., Meyer, M. V., Qureshi, N., and Takayama, K. (1985) J. Biol. Chem. 260, 16080-16088). Using [4'-32P]lipid IVA as the probe, we now demonstrate the existence of cytoplasmic KDO-transferases in Escherichia coli capable of attaching 2 KDO residues, derived from CMP-KDO, to lipid IVA. A partial purification has been developed to obtain a cytoplasmic subfraction that adds these 2 KDO residues with a 90% yield. The product is shown to have the stoichiometry of (KDO)2-IVA by fast atom bombardment mass spectrometry and NMR spectroscopy. The partially purified enzyme can utilize alternative lipid-disaccharide cosubstrates bearing five or six fatty acyl chains, but it has an absolute requirement for a monophosphate residue at position 4' of the lipid acceptor. When reincubated with a crude cytoplasmic fraction, a nucleoside triphosphate and Mg2+, (KDO)2-IVA is rapidly metabolized to more polar substances, the identity of which is unknown. The KDO-transferase(s) described in the present study should be very useful for the semisynthetic preparation of complex lipopolysaccharide substructures and analogs.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2651435 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
WaaA
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
This enzyme sequentially transfers two residues of KDO (3-deoxy-D-manno-octulosonic acid) from CMP-KDO to tetraacyldisaccharide 1,4’ bisphosphate (lipid IVA) to form KDO2-lipid IVA. It is assayed using [4’-32P]lipid IVA and CMP-KDO. The former substrate was prepared and partially purified by the investigators while the latter is generated in situ (using CMP-Kdo synthase, Kdo and CTP) since it is extremely unstable. Product was separated from reactants by thin layer chromatography and quantified by scintillation counting (pg. 6957; see also, Figs. 5, 7, and 9).
|
complete
|-
|
WaaA
|

|
[[Category:GO:0016020_!_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016020_!_membrane GO:0016020]
|
membrane
|
IDA: Inferred from Direct Assay
|

|
C
|
When membranes are prepared using high ionic strength buffers, the KDO transferase activity is equally distributed in the 105,000 x g pellet and supernatant fractions. Using the method of Munson et al. (PMID:342527), all of the activity fractionates with membranes (pg. 6959). In subsequent work (PMID:1577828) the WaaA transferase was purified from membranes obtained after disruption of cells by passage through a French pressure cell.
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.D47ab360838667&page=129474&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

PMID:2651435

2008-11-10T16:39:43Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Brozek, KA, Hosaka, K, Robertson, AD and Raetz, CR''' (1989) Biosynthesis of lipopolysaccharide in Escherichia coli. Cytoplasmic enzymes that attach 3-deoxy-D-manno-octulosonic acid to lipid A.''J. Biol. Chem.'' '''264''':6956-66

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || Previous studies in our laboratory led to the elucidation of the covalent structure of a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (designated lipid IVA), that accumulates at 42 degrees C in temperature-sensitive mutants defective in 3-deoxy-D-manno-octulosonic acid (KDO) biosynthesis (Raetz, C. R. H., Purcell, S., Meyer, M. V., Qureshi, N., and Takayama, K. (1985) J. Biol. Chem. 260, 16080-16088). Using [4'-32P]lipid IVA as the probe, we now demonstrate the existence of cytoplasmic KDO-transferases in Escherichia coli capable of attaching 2 KDO residues, derived from CMP-KDO, to lipid IVA. A partial purification has been developed to obtain a cytoplasmic subfraction that adds these 2 KDO residues with a 90% yield. The product is shown to have the stoichiometry of (KDO)2-IVA by fast atom bombardment mass spectrometry and NMR spectroscopy. The partially purified enzyme can utilize alternative lipid-disaccharide cosubstrates bearing five or six fatty acyl chains, but it has an absolute requirement for a monophosphate residue at position 4' of the lipid acceptor. When reincubated with a crude cytoplasmic fraction, a nucleoside triphosphate and Mg2+, (KDO)2-IVA is rapidly metabolized to more polar substances, the identity of which is unknown. The KDO-transferase(s) described in the present study should be very useful for the semisynthetic preparation of complex lipopolysaccharide substructures and analogs.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2651435 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
WaaA
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
This enzyme sequentially transfers two residues of KDO (3-deoxy-D-manno-octulosonic acid) from CMP-KDO to tetraacyldisaccharide 1,4’ bisphosphate (lipid IVA) to form KDO2-lipid IVA. It is assayed using [4’-32P]lipid IVA and CMP-KDO. The former substrate was prepared and partially purified by the investigators while the latter is generated in situ (using CMP-Kdo synthase, Kdo and CTP) since it is extremely unstable. Product was separated from reactants by thin layer chromatography and quantified by scintillation counting (pg. 6957; see also, Figs. 5, 7, and 9).
|
complete
|-
|
WaaA
|

|
[[Category:]][http://gowiki.tamu.edu/wiki/index.php/Category: GO:001620]
|

|
IDA: Inferred from Direct Assay
|

|

|
When membranes are prepared using high ionic strength buffers, the KDO transferase activity is equally distributed in the 105,000 x g pellet and supernatant fractions. Using the method of Munson et al. (PMID:342527), all of the activity fractionates with membranes (pg. 6959). In subsequent work (PMID:1577828) the WaaA transferase was purified from membranes obtained after disruption of cells by passage through a French pressure cell.
|
required field missing

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.D47ab360838667&page=129474&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

waaA:Quickview

2008-11-10T16:28:55Z

D-struck: Table edited by D-struck via TableEdit

waaA:Gene Product(s)

2008-11-10T16:27:39Z

D-struck: Table edited by D-struck via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|waaA}}{{Tab|Gene|waaA}}{{Tab On|Gene Product(s)|waaA}}{{Tab|Expression|waaA}}{{Tab|Evolution|waaA}}{{Tab|On One Page|waaA}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Standard name
||
KdtA product
|-
!align=left {{table heading style}} |Synonyms
||
3-deoxy-D-manno-octulosonic-acid transferase (KDO transferase) product<ref name='LIB:Riley_2006'/>, B3633 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, WaaA product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, KdtA product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
KDOTRANS-MONOMER<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

|-
!align=left {{table heading style}} |EC number (for enzymes)
||
** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=-2 -2]<ref name='LIB:Riley_2006'/>

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27830.U46aaf7b092b22&page=27830&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> {{Product_GO_help}}

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|

|

|

|
C
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|

|

|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0009244_!_lipopolysaccharide_core_region_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009244_!_lipopolysaccharide_core_region_biosynthetic_process GO:0009244]
|
lipopolysaccharide core region biosynthetic process
|

|

|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0009248_!_K_antigen_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009248_!_K_antigen_biosynthetic_process GO:0009248]
|
K antigen biosynthetic process
|

|

|

|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0030113_!_capsule_(sensu_Bacteria)]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0030113_!_capsule_(sensu_Bacteria) GO:0030113]
|
capsule (sensu Bacteria)
|

|

|

|
C
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase_activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] 
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR007507 InterPro:IPR007507]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0005529_!_sugar_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005529_!_sugar_binding GO:0005529]
|
sugar_binding
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] 
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR007507 InterPro:IPR007507]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0005975_!_carbohydrate_metabolic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005975_!_carbohydrate_metabolic_process GO:0005975]
|
carbohydrate_metabolism
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] 
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR007507 InterPro:IPR007507]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0009058_!_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009058_!_biosynthetic_process GO:0009058]
|
biosynthesis
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002] 
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR001296 InterPro:IPR001296]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0009103_!_lipopolysaccharide_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009103_!_lipopolysaccharide_biosynthetic_process GO:0009103]
|
lipopolysaccharide_biosynthesis
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004] 
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0448 SP_KW:KW-0448]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0016020_!_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016020_!_membrane GO:0016020]
|
membrane
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004] 
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0397 SP_KW:KW-0397]
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0016020_!_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016020_!_membrane GO:0016020]
|
membrane
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004] 
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0472 SP_KW:KW-0472]
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0016021_!_integral_to_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016021_!_integral_to_membrane GO:0016021]
|
integral_to_membrane
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004] 
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0812 SP_KW:KW-0812]
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase_activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004] 
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0808 SP_KW:KW-0808]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
[[PMID:2651435]]<ref name=PMID:2651435/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete
|-
|

|
[[Category:GO:0016020_!_membrane]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016020_!_membrane GO:0016020]
|
membrane
|
[[PMID:2651435]]<ref name=PMID:2651435/>
|
IDA: Inferred from Direct Assay
|

|
C
|

|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[[PMID:7499229]]<ref name=PMID:7499229/>
|
IMP: Inferred from Mutant Phenotype
|

|
P
|

|
complete
|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
[[PMID:1577828]]<ref name=PMID:1577828/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27830.N46aaf7b1d3944&page=27830&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Partner Type!!Partner!!Notes

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27830.C46aaf7d6239c7&page=27830&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table] || ||
|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Compartment!!Description!!Evidence!!Notes

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27830.H46a884cbaabc0&page=27830&pagename={{FULLPAGENAMEE}}&type=&template=Product_localization_table edit table] || || ||
|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Sequence
||
[http://biocyc.org/ECOLI/sequence-aa?object=KDOTRANS-MONOMER at EcoCyc]
<pre>MLELLYTALL YLIQPLIWIR LWVRGRKAPA YRKRWGERYG FYRHPLKPGG IMLHSVSVGE
TLAAIPLVRA LRHRYPDLPI TVTTMTPTGS ERVQSAFGKD VQHVYLPYDL PDALNRFLNK
VDPKLVLIME TELWPNLIAA LHKRKIPLVI ANARLSARSA AGYAKLGKFV RRLLRRITLI
AAQNEEDGAR FVALGAKNNQ VTVTGSLKFD ISVTPQLAAK AVTLRRQWAP HRPVWIATST
HEGEESVVIA AHQALLQQFP NLLLILVPRH PERFPDAINL VRQAGLSYIT RSSGEVPSTS
TQVVVGDTMG ELMLLYGIAD LAFVGGSLVE RGGHNPLEAA AHAIPVLMGP HTFNFKDICA
RLEQASGLIT VTDATTLAKE VSSLLTDADY RSFYGRHAVE VLYQNQGALQ RLLQLLEPYL
PPKTH</pre>
|-
!align=left {{table heading style}} |Mol. Wt
||
47.29 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!align=left {{table heading style}} |pI
||
9.89<ref name='LIB:EcoCyc10.6'/>
|-
!align=left {{table heading style}} |Extinction coefficient
||
55350 - 55475 (calc based on 15 Y, 6 W, and 1 C residues)

|-class='sortbottom'
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|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Type!!Residues!!Description!!Notes

|-class='sortbottom'
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|}

{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>waaA
562</beststructure>

|-
!align=left {{table heading style}} |Models
||

View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=waaA&sub=keywordSearc EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=waaA&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27830.Q46a884cd4dd82&page=27830&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table] ||
|}

{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Resource type!!Source!!Notes/Reference

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27830.B46aaf7d973f3b&page=27830&pagename={{FULLPAGENAMEE}}&type=&template=Product_resource_table edit table] || ||
|}

{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=KDOTRANS-MONOMER EcoCyc:KDOTRANS-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P0AC75|UniProt:P0AC75]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF04413 PFAM:PF04413]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_418090 RefSeq:NP_418090]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=P0AC75 ModBase:P0AC75]
|

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27830.W46aaf7d9d82a4&page=27830&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27830.O46a884d0ce864&page=27830&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0009245_!_lipid A biosynthetic process]][[Category:GO:0009244_!_lipopolysaccharide core region biosynthetic process]][[Category:GO:0009248_!_K antigen biosynthetic process]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]][[Category:GO:0030113_!_capsule (sensu Bacteria)]]
[[Category:Proteins]]

PMID:12000770

2008-10-28T15:59:32Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Babinski, KJ, Ribeiro, AA and Raetz, CR''' (2002) The Escherichia coli gene encoding the UDP-2,3-diacylglucosamine pyrophosphatase of lipid A biosynthesis.''J. Biol. Chem.'' '''277''':25937-46

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || UDP-2,3-diacylglucosamine hydrolase is believed to catalyze the fourth step of lipid A biosynthesis in Escherichia coli. This reaction involves pyrophosphate bond hydrolysis of the precursor UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate and UMP. To identify the gene encoding this hydrolase, E. coli lysates generated with individual lambda clones of the ordered Kohara library were assayed for overexpression of the enzyme. The sequence of lambda clone 157[6E7], promoting overproduction of hydrolase activity, was examined for genes encoding hypothetical proteins of unknown function. The amino acid sequence of one such open reading frame, ybbF, is 50.5% identical to a Haemophilus influenzae hypothetical protein and is also conserved in most other Gram-negative organisms, but is absent in Gram-positives. Cell extracts prepared from cells overexpressing ybbF behind the T7lac promoter have approximately 540 times more hydrolase activity than cells with vector alone. YbbF was purified to approximately 60% homogeneity, and its catalytic properties were examined. Enzymatic activity is maximal at pH 8 and is inhibited by 0.01% (or more) Triton X-100. The apparent K(m) for UDP-2,3-diacylglucosamine is 62 microm. YbbF requires a diacylated substrate and does not cleave CDP-diacylglycerol. (31)P NMR studies of the UMP product generated from UDP-2,3-diacylglucosamine in the presence of 40% H(2)180 show that the enzyme attacks the alpha-phosphate group of the UDP moiety. Because ybbF encodes the specific UDP-2,3-diacylglucosamine hydrolase involved in lipid A biosynthesis, it is now designated lpxH.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12000770 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxH
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|
IDA: Inferred from Direct Assay
|

|
C
|
In strains overproducing the lpxH gene, approximately 65% of the LpxH activity is recovered in the 150,000 x g supernatant. However, if MnCl2 is present, most of the activity fractionates with membranes (see Table III and pg. 25944).
|
complete
|-
|
LpxH
|

|
[[Category:GO:0008758_!_UDP-2,3-diacylglucosamine_hydrolase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008758_!_UDP-2,3-diacylglucosamine_hydrolase_activity GO:0008758]
|
UDP-2,3-diacylglucosamine hydrolase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
The enzyme is assayed using [beta-32P]UDP-2,3diacylglucosamine as the substrate. The product, [32P]2,3diacylglucosamine-1-phosphate, was quantified after thin layer chromatography using a PhosphorImager. The second product of the reaction is UMP. The substrate is not commercially available but its preparation is described (pg. 25938).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.B47a96cdce5628&page=118586&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

PMID:3531165

2008-10-28T15:56:03Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Crowell, DN, Anderson, MS and Raetz, CR''' (1986) Molecular cloning of the genes for lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase in Escherichia coli.''J. Bacteriol.'' '''168''':152-9

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || Several enzymes have been discovered recently in crude extracts of Escherichia coli that appear to be involved in the biosynthesis of the lipid A component of lipopolysaccharide. Two of these are lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase. Lipid A disaccharide synthase activity is barely detectable in cells harboring a lesion in the lpxB (pgsB) gene. We subcloned the lpxB gene from plasmid pLC26-43 of the Clarke and Carbon collection (L. Clarke and J. Carbon, Cell 9:91-99, 1976) and localized it to a 1.7-kilobase-pair fragment of DNA counterclockwise of dnaE on the E. coli chromosome. Furthermore, we discovered a new gene (lpxA) located adjacent to and counterclockwise of lpxB that encodes or controls UDP-N-acetylglucosamine acyltransferase. Our data prove that lpxB and lpxA are transcribed in the clockwise direction and suggest that they may be cotranscribed.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3531165 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxA and LpxB
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
IEP: Inferred from Expression Pattern
|

|
P
|
Expression of genes on a DNA fragment derived from pLC25-43 from the Clarke-Carbon collection (PMID:788919) was found to result in the accumulation of Lipid A disaccharide synthase (LpxB) and UDP-N-acetylglucosamine acyltransferase (LpxA) activity (Table 2).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.K46c31e9155227&page=112087&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

PMID:3531165

2008-10-27T16:51:38Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Crowell, DN, Anderson, MS and Raetz, CR''' (1986) Molecular cloning of the genes for lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase in Escherichia coli.''J. Bacteriol.'' '''168''':152-9

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || Several enzymes have been discovered recently in crude extracts of Escherichia coli that appear to be involved in the biosynthesis of the lipid A component of lipopolysaccharide. Two of these are lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase. Lipid A disaccharide synthase activity is barely detectable in cells harboring a lesion in the lpxB (pgsB) gene. We subcloned the lpxB gene from plasmid pLC26-43 of the Clarke and Carbon collection (L. Clarke and J. Carbon, Cell 9:91-99, 1976) and localized it to a 1.7-kilobase-pair fragment of DNA counterclockwise of dnaE on the E. coli chromosome. Furthermore, we discovered a new gene (lpxA) located adjacent to and counterclockwise of lpxB that encodes or controls UDP-N-acetylglucosamine acyltransferase. Our data prove that lpxB and lpxA are transcribed in the clockwise direction and suggest that they may be cotranscribed.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3531165 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxA and LpxB
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
IEP: Inferred from Expression Pattern
|

|
P
|
Expression of genes on a DNA fragment derived from pLC25-43 from the Clarke-Carbon collection (PMID:???) was found to result in the accumulation of Lipid A disaccharide synthase (LpxB) and UDP-N-acetylglucosamine acyltransferase (LpxA) activity (Table 2).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.K46c31e9155227&page=112087&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

PMID:6370995

2008-10-27T16:49:48Z

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{{RightTOC}}

 '''Ray, BL, Painter, G and Raetz, CR''' (1984) The biosynthesis of gram-negative endotoxin. Formation of lipid A disaccharides from monosaccharide precursors in extracts of Escherichia coli.''J. Biol. Chem.'' '''259''':4852-9

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || We have discovered an enzyme in the cytosol of Escherichia coli that generates lipid A disaccharides from monosaccharide precursors by the following route: 2,3-diacyl-GlcN-1-P + UDP-2,3-diacyl-GlcN---- 2,3-diacyl-GlcN (beta, 1----6) 2,3-diacyl-GlcN-1-P + UDP. Previous studies from our laboratory have documented the presence in vivo of the precursors 2,3-diacylglucosamine 1-phosphate (2,3-diacyl-GlcN-1-P) (lipid X of E. coli) and UDP-2,3-diacylglucosamine (UDP-2,3-diacyl-GlcN) (Bulawa, C.E., and Raetz, C.R.H.J. Biol. Chem. 259, 4846-4851). Both substrates are novel glucosamine-derived phospholipids, acylated with beta-hydroxymyristoyl moieties, and they accumulate in E. coli mutants defective in the pgsB gene. Synthetic ADP-, GDP-, and CDP-2,3-diacylglucosamines are inefficient substrates compared to the naturally occurring UDP derivative. The free-acid form of the tetraacyldisaccharide 1-phosphate product (C68H129N2O20P) that is generated in vitro has Mr = 1325.74 as judged by fast atom bombardment mass spectrometry. Mild acid hydrolysis (0.1 M HCl for 30 min at 100 degrees C) liberates greater than 95% of the phosphate moiety as Pi. Detailed analysis by 1H and 13C NMR spectroscopy confirms the presence of a phosphate residue at position 1 of the disaccharide, an alpha-anomeric configuration at the reducing end, and a beta, 1----6 linkage between the two glucosamines. Importantly the disaccharide 1-phosphate synthase is missing in extracts of E. coli strains harboring the pgsB1 mutation, consistent with the massive accumulation of 2,3-diacyl-GlcN-1-P and UDP-2,3-diacyl-GlcN in vivo. The enzymatic reaction reported here represents a major biosynthetic route for the formation of lipid A disaccharides in E. coli and other Gram-negative bacteria. An in vitro system for the biosynthesis of lipid A disaccharides has not been described previously.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6370995 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxB
|

|
[[Category:GO:0008915_!_lipid-A-disaccharide_synthase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008915_!_lipid-A-disaccharide_synthase_activity GO:0008915]
|
lipid-A-disaccharide synthase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
The conversion of UDP-2,3diacylglucosamine and [32P]2,3diacylglucosamine-1-phosphate into [32P]2,3-diacylglucosamine-(beta-1,6)2,3-diacylglucosamine-1-phosphate (lipid A disaccharide or tetraacyldisaccharide-1-phosphate) is monitored by thin layer chromatography and scintillation counting (Fig. 3). Product formation can also be followed using a differential extraction procedure (Fig. 4) obviating the need for the chromatographic step.
|
complete
|-
|
LpxB
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
IMP: Inferred from Mutant Phenotype
|

|
P
|
Extracts prepared from a strain with the pgsB1 (psgB has been renamed lpxB) mutation are markedly deficient in their ability to synthesize the lipid A disaccharide from its precursors (Fig. 9).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Y46c32c82f008a&page=112116&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

lpxB:Gene Product(s)

2008-10-27T16:46:48Z

D-struck: Table edited by D-struck via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|lpxB}}{{Tab|Gene|lpxB}}{{Tab On|Gene Product(s)|lpxB}}{{Tab|Expression|lpxB}}{{Tab|Evolution|lpxB}}{{Tab|On One Page|lpxB}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|{{Prettytable}}
|-
!{{table heading style}} |Standard name
|
LpxB product
|-
!{{table heading style}} |Synonyms
|
tetraacyldisaccharide-1-P synthase product<ref name='LIB:Riley_2006'/>, B0182 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, PgsB product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, LpxB product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!{{table heading style}} |Product description
|
LpxB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;

Component of ; [[:Category:Complex:lipid A disaccharide synthase|lipid A disaccharide synthase]]<ref name='LIB:EcoCyc11.1'/>

|-
!{{table heading style}} |EC number (for enzymes)
|
** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.182 2.4.1.182]<ref name='LIB:Riley_2006'/>
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19932.F46aa21bbaaf31&page=19932&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> {{Product_GO_help}}

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0005515_!_protein_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005515_!_protein_binding GO:0005515]
|
protein binding
|
[[PMID:15690043]]<ref name=PMID:15690043/>
|
IPI: Inferred from Physical Interaction
|
[http://www.ebi.uniprot.org/entry/P0A6N1 UniProtKB:P0A6N1]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008610_!_lipid_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008610_!_lipid_biosynthetic_process GO:0008610]
|
lipid biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0444 SP_KW:KW-0444]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008915_!_lipid-A-disaccharide_synthase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008915_!_lipid-A-disaccharide_synthase_activity GO:0008915]
|
lipid-A-disaccharide synthase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00392
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008915_!_lipid-A-disaccharide_synthase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008915_!_lipid-A-disaccharide_synthase_activity GO:0008915]
|
lipid-A-disaccharide synthase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR003835 InterPro:IPR003835]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008915_!_lipid-A-disaccharide_synthase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008915_!_lipid-A-disaccharide_synthase_activity GO:0008915]
|
lipid-A-disaccharide synthase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000003 GO_REF:0000003]
|
IEA: Inferred from Electronic Annotation
|
EC:2.4.1.182
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00392
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR003835 InterPro:IPR003835]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0441 SP_KW:KW-0441]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0808 SP_KW:KW-0808]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016757_!_transferase_activity,_transferring_glycosyl_groups]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016757_!_transferase_activity,_transferring_glycosyl_groups GO:0016757]
|
transferase activity, transferring glycosyl groups
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0328 SP_KW:KW-0328]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|

|

|

|
C
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting GO:0042280]
|
cell surface antigen activity, host-interacting
|

|

|

|
F
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0008915_!_lipid-A-disaccharide_synthase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008915_!_lipid-A-disaccharide_synthase_activity GO:0008915]
|
lipid-A-disaccharide synthase activity
|
[[PMID:6370995]]<ref name=PMID:6370995/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[[PMID:6370995]]<ref name=PMID:6370995/>
|
IMP: Inferred from Mutant Phenotype
|

|
P
|

|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[[PMID:3531165]]<ref name=PMID:3531165/>
|
IEP: Inferred from Expression Pattern
|

|
P
|

|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19932.O46aa21bd0f0eb&page=19932&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>


{|class = 'sortable' {{Prettytable}} id='152752'
|- {{table heading style}}
!Partner Type!!Partner!!Notes
|-
|
Protein
|
Subunits of [[:Category:Complex:lipid A disaccharide synthase|lipid A disaccharide synthase]]
|
could be indirect
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19932.S46aa21d62acaa&page=19932&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' {{Prettytable}} id='102066'
|- {{table heading style}}
!Compartment!!Description!!Evidence!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19932.X46a7b322830fd&page=19932&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|{{Prettytable}}
|-
!{{table heading style}} |Sequence
|
[http://biocyc.org/ECOLI/sequence-aa?object=LIPIDADISACCHARIDESYNTH-MONOMER at EcoCyc]
<pre>MTEQRPLTIA LVAGETSGDI LGAGLIRALK EHVPNARFVG VAGPRMQAEG CEAWYEMEEL
AVMGIVEVLG RLRRLLHIRA DLTKRFGELK PDVFVGIDAP DFNITLEGNL KKQGIKTIHY
VSPSVWAWRQ KRVFKIGRAT DLVLAFLPFE KAFYDKYNVP CRFIGHTMAD AMPLDPDKNA
ARDVLGIPHD AHCLALLPGS RGAEVEMLSA DFLKTAQLLR QTYPDLEIVV PLVNAKRREQ
FERIKAEVAP DLSVHLLDGM GREAMVASDA ALLASGTAAL ECMLAKCPMV VGYRMKPFTF
WLAKRLVKTD YVSLPNLLAG RELVKELLQE ECEPQKLAAA LLPLLANGKT SHAMHDTFRE
LHQQIRCNAD EQAAQAVLEL AQ</pre>
|-
!{{table heading style}} |Mol. Wt
|
42.38 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |pI
|
7<ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |Extinction coefficient
|
32430 - 33305 (calc based on 7 Y, 4 W, and 7 C residues)
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19932.D46aa21d83fc18&page=19932&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


{|class = 'sortable' {{Prettytable}} id='152754'
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19932.G46aa21d898153&page=19932&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>lpxB
562</beststructure>

|-
!align=left {{table heading style}} |Models
||

View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=lpxB&sub=keywordSearc EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=lpxB&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19932.C46a7b3241a43d&page=19932&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table] ||
|}

{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152755'
|- {{table_heading_style}}
!Resource type!!Source!!Notes/Reference
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19932.J46aa21d937350&page=19932&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152756'
|- {{table_heading_style}}
!Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=LIPIDADISACCHARIDESYNTH-MONOMER EcoCyc:LIPIDADISACCHARIDESYNTH-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P10441|UniProt:P10441]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF02684 PFAM:PF02684]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_414724 RefSeq:NP_414724]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=P10441 ModBase:P10441]
|

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19932.B46aa21d980b92&page=19932&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='102069'
|- {{table_heading_style}}
!Name!!URL!!Comments
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19932.E46a7b327affc0&page=19932&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0009245_!_lipid A biosynthetic process]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]][[Category:Complex:lipid A disaccharide synthase]]

PMID:12000771

2008-10-27T16:38:38Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Babinski, KJ, Kanjilal, SJ and Raetz, CR''' (2002) Accumulation of the lipid A precursor UDP-2,3-diacylglucosamine in an Escherichia coli mutant lacking the lpxH gene.''J. Biol. Chem.'' '''277''':25947-56

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || The lpxH gene encodes the UDP-2,3-diacylglucosamine-specific pyrophosphatase that catalyzes the fourth step of lipid A biosynthesis in Escherichia coli. To confirm the function of lpxH, we constructed KB21/pKJB5. This strain contains a kanamycin insertion element in the chromosomal copy of lpxH, complemented by plasmid pKJB5, which is temperature-sensitive for replication and harbors lpxH(+). KB21/pKJB5 grows at 30 degrees C but loses viability at 44 degrees C, demonstrating that lpxH is essential. CDP-diglyceride hydrolase (Cdh) catalyzes the same reaction as LpxH in vitro but is non-essential and cannot compensate for the absence of LpxH. The presence of Cdh in cell extracts interferes with the LpxH assay. We therefore constructed KB25/pKJB5, which contains both an in-frame deletion of cdh and a kanamycin insertion mutation in lpxH, covered by pKJB5. When KB25/pKJB5 cells are grown at 44 degrees C, viability is lost, and all in vitro LpxH activity is eliminated. A lipid migrating with synthetic UDP-2,3-diacylglucosamine accumulates in KB25/pKJB5 following loss of the covering plasmid at 44 degrees C. This material was converted to the expected products, 2,3-diacylglucosamine 1-phosphate and UMP, by LpxH. Pseudomonas aeruginosa contains two proteins with sequence similarity to E. coli LpxH. The more homologous protein catalyzes UDP-2,3-diacylglucosamine hydrolysis in vitro. The corresponding gene complements KB25/pKJB5 at 44 degrees C, but the less homologous gene does not. The accumulation of UDP-2,3-diacylglucosamine in our lpxH mutant is consistent with the observation that the lipid A disaccharide synthase LpxB, the next enzyme in the pathway, cannot condense two UDP-2,3-diacylglucosamine molecules, but instead utilizes UDP-2,3-diacylglucosamine as its donor and 2,3-diacylglucosamine 1-phosphate as its acceptor.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12000771 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxH
|

|
[[Category:GO:0008758_!_UDP-2,3-diacylglucosamine_hydrolase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008758_!_UDP-2,3-diacylglucosamine_hydrolase_activity GO:0008758]
|
UDP-2,3-diacylglucosamine hydrolase activity
|
IMP: Inferred from Mutant Phenotype
|

|
F
|
The construction of a strain with a kanR insertion in the lpxH gene and complemented by a plasmid that is temperature sensitive for replication and carrying lpxH gene is described. At elevated temperature, this strain is non-viable and accumulates UDP-2,3diacylglucosamine (Fig. 5).
|
complete
|-
|
LpxH
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
IMP: Inferred from Mutant Phenotype
|

|
P
|
CDP-diglyceride hydrolase encoded by the cdh gene cannot substitute for LpxH (Fig. 2) even though it can hydrolyze UDP-2,3diacylglucosamine in vitro (Fig. 1).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.H46c34a8eee803&page=112184&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

lpxH:Gene Product(s)

2008-10-27T16:36:32Z

D-struck: Table edited by D-struck via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|lpxH}}{{Tab|Gene|lpxH}}{{Tab On|Gene Product(s)|lpxH}}{{Tab|Expression|lpxH}}{{Tab|Evolution|lpxH}}{{Tab|On One Page|lpxH}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Standard name
||
LpxH product
|-
!align=left {{table heading style}} |Synonyms
||
UDP-2,3-diacylglucosamine pyrophosphatase product<ref name='LIB:Riley_2006'/>, B0524 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YbbF product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, LpxH product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
EG12666-MONOMER<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

|-
!align=left {{table heading style}} |EC number (for enzymes)
||
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.- 3.6.1.-]<ref name='LIB:Riley_2006'/>
*[[kegg:ko00540.html|LPS biosynthesis]]

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20731.Q46aa34d7895ca&page=20731&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> {{Product_GO_help}}

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR010138 InterPro:IPR010138]
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0963 SP_KW:KW-0963]
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008758_!_UDP-2,3-diacylglucosamine_hydrolase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008758_!_UDP-2,3-diacylglucosamine_hydrolase_activity GO:0008758]
|
UDP-2,3-diacylglucosamine hydrolase activity
|
[[PMID:12000770]]<ref name=PMID:12000770/>
|
IDA: Inferred from Direct Assay
|

|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00575
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
[http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR010138 InterPro:IPR010138]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
[http://www.uniprot.org/keywords/KW-0441 SP_KW:KW-0441]
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[[PMID:12000770]]<ref name=PMID:12000770/>
|
IDA: Inferred from Direct Assay
|

|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting GO:0042280]
|
cell surface antigen activity, host-interacting
|

|

|

|
F
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0008758_!_UDP-2,3-diacylglucosamine_hydrolase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008758_!_UDP-2,3-diacylglucosamine_hydrolase_activity GO:0008758]
|
UDP-2,3-diacylglucosamine hydrolase activity
|
[[PMID:12000771]]<ref name=PMID:12000771/>
|
IMP: Inferred from Mutant Phenotype
|

|
F
|

|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[[PMID:12000771]]<ref name=PMID:12000771/>
|
IMP: Inferred from Mutant Phenotype
|

|
P
|

|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20731.P46aa34d8834fa&page=20731&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>


{|class = 'sortable' {{Prettytable}} id='155202'
|- {{table heading style}}
!Partner Type!!Partner!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20731.D46aa34e1abb27&page=20731&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' {{Prettytable}} id='104324'
|- {{table heading style}}
!Compartment!!Description!!Evidence!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20731.L46a7c65cf1c75&page=20731&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|{{Prettytable}}
|-
!{{table heading style}} |Sequence
|
[http://biocyc.org/ECOLI/sequence-aa?object=EG12666-MONOMER at EcoCyc]
<pre>MATLFIADLH LCVEEPAITA GFLRFLAGEA RKADALYILG DLFEAWIGDD DPNPLHRKMA
AAIKAVSDSG VPCYFIHGNR DFLLGKRFAR ESGMTLLPEE KVLELYGRRV LIMHGDTLCT
DDAGYQAFRA KVHKPWLQTL FLALPLFVRK RIAARMRANS KEANSSKSLA IMDVNQNAVV
SAMEKHQVQW LIHGHTHRPA VHELIANQQP AFRVVLGAWH TEGSMVKVTA DDVELIHFPF</pre>
|-
!{{table heading style}} |Mol. Wt
|
26.89 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |pI
|

|-
!{{table heading style}} |Extinction coefficient
|
27960 - 28335 (calc based on 4 Y, 4 W, and 3 C residues)
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20731.K46aa34e36b594&page=20731&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


{|class = 'sortable' {{Prettytable}} id='155204'
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20731.M46aa34e3c581e&page=20731&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Structures
||
<beststructure>lpxH
562</beststructure>

|-
!align=left {{table heading style}} |Models
||

View models at:
* [http://dragon.bio.purdue.edu/ecolpredict2/localsearch/cgi-bin/search3.cgi?q=lpxH&sub=keywordSearc EcoliPredict]
* [http://modbase.compbio.ucsf.edu/modbase-cgi/model_search.cgi?searchmode=default&displaymode=moddetail&searchproperties=ALL&searchvalue=lpxH&organism=562&organismtext= ModBase]
* [http://bioinf.cs.ucl.ac.uk/psipred/ PSI-PRED]
* [http://www.ebi.ac.uk/msd-srv/pqs/ Protein Quaternary Structure] (PQS)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20731.Y46a7c65e8c71d&page=20731&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table] ||
|}

{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='155205'
|- {{table_heading_style}}
!Resource type!!Source!!Notes/Reference
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20731.Q46aa34e467392&page=20731&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='155206'
|- {{table_heading_style}}
!Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG12666-MONOMER EcoCyc:EG12666-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P43341|UniProt:P43341]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF00149 PFAM:PF00149]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_415057 RefSeq:NP_415057]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=P43341 ModBase:P43341]
|

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20731.A46aa34e4b1d56&page=20731&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='104327'
|- {{table_heading_style}}
!Name!!URL!!Comments
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.20731.T46a7c662087c8&page=20731&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0009245_!_lipid A biosynthetic process]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]]

PMID:12000770

2008-10-27T16:32:43Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Babinski, KJ, Ribeiro, AA and Raetz, CR''' (2002) The Escherichia coli gene encoding the UDP-2,3-diacylglucosamine pyrophosphatase of lipid A biosynthesis.''J. Biol. Chem.'' '''277''':25937-46

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || UDP-2,3-diacylglucosamine hydrolase is believed to catalyze the fourth step of lipid A biosynthesis in Escherichia coli. This reaction involves pyrophosphate bond hydrolysis of the precursor UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate and UMP. To identify the gene encoding this hydrolase, E. coli lysates generated with individual lambda clones of the ordered Kohara library were assayed for overexpression of the enzyme. The sequence of lambda clone 157[6E7], promoting overproduction of hydrolase activity, was examined for genes encoding hypothetical proteins of unknown function. The amino acid sequence of one such open reading frame, ybbF, is 50.5% identical to a Haemophilus influenzae hypothetical protein and is also conserved in most other Gram-negative organisms, but is absent in Gram-positives. Cell extracts prepared from cells overexpressing ybbF behind the T7lac promoter have approximately 540 times more hydrolase activity than cells with vector alone. YbbF was purified to approximately 60% homogeneity, and its catalytic properties were examined. Enzymatic activity is maximal at pH 8 and is inhibited by 0.01% (or more) Triton X-100. The apparent K(m) for UDP-2,3-diacylglucosamine is 62 microm. YbbF requires a diacylated substrate and does not cleave CDP-diacylglycerol. (31)P NMR studies of the UMP product generated from UDP-2,3-diacylglucosamine in the presence of 40% H(2)180 show that the enzyme attacks the alpha-phosphate group of the UDP moiety. Because ybbF encodes the specific UDP-2,3-diacylglucosamine hydrolase involved in lipid A biosynthesis, it is now designated lpxH.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12000770 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxH
|

|
[[Category:GO:0008758_!_UDP-2,3-diacylglucosamine_hydrolase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008758_!_UDP-2,3-diacylglucosamine_hydrolase_activity GO:0008758]
|
UDP-2,3-diacylglucosamine hydrolase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
The enzyme is assayed using [beta-32P]UDP-2,3diacylglucosamine as the substrate. The product, [32P]2,3diacylglucosamine-1-phosphate, was quantified after thin layer chromatography using a PhosphoImager. The second product of the reaction is UMP. The substrate is not commercially available but its preparation is described (pg. 25938).
|
complete
|-
|
LpxH
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|
IDA: Inferred from Direct Assay
|

|
C
|
In strains overproducing the lpxH gene, approximately 65% of the LpxH activity is recovered in the 150,000 x g supernatant. However, if MnCl2 is present, most of the activity fractionates with membranes (see Table III and pg. 25944).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.B47a96cdce5628&page=118586&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

PMID:12000770

2008-10-27T16:30:37Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Babinski, KJ, Ribeiro, AA and Raetz, CR''' (2002) The Escherichia coli gene encoding the UDP-2,3-diacylglucosamine pyrophosphatase of lipid A biosynthesis.''J. Biol. Chem.'' '''277''':25937-46

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || UDP-2,3-diacylglucosamine hydrolase is believed to catalyze the fourth step of lipid A biosynthesis in Escherichia coli. This reaction involves pyrophosphate bond hydrolysis of the precursor UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate and UMP. To identify the gene encoding this hydrolase, E. coli lysates generated with individual lambda clones of the ordered Kohara library were assayed for overexpression of the enzyme. The sequence of lambda clone 157[6E7], promoting overproduction of hydrolase activity, was examined for genes encoding hypothetical proteins of unknown function. The amino acid sequence of one such open reading frame, ybbF, is 50.5% identical to a Haemophilus influenzae hypothetical protein and is also conserved in most other Gram-negative organisms, but is absent in Gram-positives. Cell extracts prepared from cells overexpressing ybbF behind the T7lac promoter have approximately 540 times more hydrolase activity than cells with vector alone. YbbF was purified to approximately 60% homogeneity, and its catalytic properties were examined. Enzymatic activity is maximal at pH 8 and is inhibited by 0.01% (or more) Triton X-100. The apparent K(m) for UDP-2,3-diacylglucosamine is 62 microm. YbbF requires a diacylated substrate and does not cleave CDP-diacylglycerol. (31)P NMR studies of the UMP product generated from UDP-2,3-diacylglucosamine in the presence of 40% H(2)180 show that the enzyme attacks the alpha-phosphate group of the UDP moiety. Because ybbF encodes the specific UDP-2,3-diacylglucosamine hydrolase involved in lipid A biosynthesis, it is now designated lpxH.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12000770 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxH
|

|
[[Category:GO:0008758_!_UDP-2,3-diacylglucosamine_hydrolase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008758_!_UDP-2,3-diacylglucosamine_hydrolase_activity GO:0008758]
|
UDP-2,3-diacylglucosamine hydrolase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
The enzyme is assayed using [beta-32P]UDP-2,3diacylglucosamine as the substrate. The product, [32P]2,3diacylglucosamine-1-phosphate, was quantified after thin layer chromatography using a PhosphoImager. The second product of the reaction is UMP. The substrate is not commercially available but its preparation is described (pg. 25938).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.B47a96cdce5628&page=118586&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

lpxH:Quickview

2008-10-27T16:28:29Z

D-struck: Table edited by D-struck via TableEdit

PMID:8366125

2008-10-16T15:48:50Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Kelly, TM, Stachula, SA, Raetz, CR and Anderson, MS''' (1993) The firA gene of Escherichia coli encodes UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase. The third step of endotoxin biosynthesis.''J. Biol. Chem.'' '''268''':19866-74

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || The possibility that the firA gene of Escherichia coli (Dicker, I. B., and Seetharam, S. (1991) Mol. Microbiol. 6, 817-823) might function in lipid A biosynthesis was examined based on its homology to the lpxA gene, which encodes UDP-N-acetylglucosamine O-acyl-transferase, the first enzyme in lipid A formation. Extracts of a temperature-sensitive firA mutant, RL-25, were assayed for their ability to acylate UDP-GlcNAc, using a coupled assay. The results suggested that extracts of RL-25 might be defective in the third enzyme of this pathway, the UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase. Living cells of RL-25 also displayed a 5-fold decreased rate of lipid A biosynthesis at the nonpermissive temperature as judged by a 32Pi incorporation assay. In order to examine N-acyltransferase activity directly, the substrate [alpha-32P]UDP-3-O-(R-3-hydroxymyristoyl)-GlcN was synthesized enzymatically. N-Acyltransferase specific activity in RL-25 extracts was reduced to less than 10% of wild-type. When the wild-type firA gene was cloned into a T7-based expression vector, N-acyltransferase specific activity increased almost 360-fold relative to wild-type extracts, demonstrating that firA is the structural gene for the enzyme. The N-acyltransferase displays absolute specificity for the R-3-OH moiety of R-3-hydroxymyristoyl-ACP, as does the O-acyltransferase, consistent with the placement of R-3-hydroxymyristate in E. coli lipid A.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8366125 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxD
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
IMP: Inferred from Mutant Phenotype
|

|
P
|
A ts mutantion in the lpxD gene (firA200) is shown to decrease the rate of lipid A synthesis at the restrictive temperature (Figs. 2 and 3).
|
complete
|-
|
LpxD
|

|
[[Category:GO:0016410_!_N-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016410_!_N-acyltransferase_activity GO:0016410]
|
N-acyltransferase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
The enzyme displays absolute specificity for the 3-OH group of R-3-hydroxymyristoyl-ACP (Fig. 9).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J47aaad6326c50&page=127496&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

PMID:3288280

2008-10-16T15:41:46Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Anderson, MS, Robertson, AD, Macher, I and Raetz, CR''' (1988) Biosynthesis of lipid A in Escherichia coli: identification of UDP-3-O-[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine as a precursor of UDP-N2,O3-bis[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine.''Biochemistry'' '''27''':1908-17

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || The lipid A disaccharide of the Escherichia coli envelope is synthesized from the two fatty acylated glucosamine derivatives UDP-N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D- glucosamine (UDP-2,3-diacyl-GlcN) and N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine 1-phosphate (2,3-diacyl-GlcN-1-P) [Ray, B. L., Painter, G., & Raetz, C. R. H. (1984) J. Biol. Chem. 259, 4852-4859]. We have previously shown that UDP-2,3-diacyl-GlcN is generated in extracts of E. coli by fatty acylation of UDP-GlcNAc, giving UDP-3-O-[(R)-3-hydroxymyristoyl]-GlcNAc as the first intermediate, which is rapidly converted to UDP-2,3-diacyl-GlcN [Anderson, M. S., Bulawa, C. E., & Raetz, C. R. H. (1985) J. Biol. Chem. 260, 15536-15541; Anderson, M. S., & Raetz, C. R. H. (1987) J. Biol. Chem. 262, 5159-5169]. We now demonstrate a novel enzyme in the cytoplasmic fraction of E. coli, capable of deacetylating UDP-3-O-[(R)-3-hydroxymyristoyl]-GlcNAc to form UDP-3-O-[(R)-3-hydroxymyristoyl]glucosamine. The covalent structure of the previously undescribed UDP-3-O-[(R)-3-hydroxymyristoyl] glucosamine intermediate was established by 1H NMR spectroscopy and fast atom bombardment mass spectrometry. This material can be made to accumulate in E. coli extracts upon incubation of UDP-3-O-[(R)-3- hydroxymyristoyl]-GlcNAc in the absence of the fatty acyl donor [(R)-3-hydroxymyristoyl]-acyl carrier protein. However, addition of the isolated deacetylation product [UDP-3-O-[(R)-3-hydroxymyristoyl] glucosamine] back to membrane-free extracts of E. coli in the presence of [(R)-3-hydroxymyristoyl]-acyl carrier protein results in rapid conversion of this compound into the more hydrophobic products UDP-2,3-diacyl-GlcN, 2,3-diacyl-GlcN-1-P, and O-[2-amino-2-deoxy-N2,O3- bis[(R)-3-hydroxytetradecanoyl]-beta-D-glucopyranosyl]-(1----6)-2-amino- 2-deoxy-N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D- glucopyranose 1-phosphate (tetra-acyldisaccharide-1-P), demonstrating its competency as a precursor. In vitro incubations using [acetyl-3H]UDP-3-O-[(R)-3-hydroxymyristoyl]-GlcNAc confirmed release of the acetyl moiety in this system as acetate, not as some other acetyl derivative. The deacetylation reaction was inhibited by 1 mM N-ethylmaleimide, while the subsequent N-acylation reaction was not. Our observations provide strong evidence that UDP-3-O-[(R)-3-hydroxymyristoyl]glucosamine is a true intermediate in the biosynthesis of UDP-2,3-diacyl-GlcN and lipid A.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3288280 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxC
|

|
[[Category:GO:0019213_!_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0019213_!_deacetylase_activity GO:0019213]
|
deacetylase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
Demonstrated by the release of [3H]-acetic acid from [3H-acetyl]- UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc (pg. 1911 and Figure 8).
|
complete
|-
|
LpxD
|

|
[[Category:GO:0016410_!_N-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016410_!_N-acyltransferase_activity GO:0016410]
|
N-acyltransferase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
Assayed using [beta-32P]UDP-3-O-[(R)-3-hydroxymyristoyl]-glucosamine and (R)-3-hydroxymyristoyl-ACP as substrates. Product was separated from the reactant by thin layer chromatography and quantified by scintillation counting. Substrates are not commercially available but their preparation is described (pg. 1911)
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Q47ab7f13d5151&page=132291&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

lpxD:Gene Product(s)

2008-10-16T15:39:11Z

D-struck: Table edited by D-struck via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|lpxD}}{{Tab|Gene|lpxD}}{{Tab On|Gene Product(s)|lpxD}}{{Tab|Expression|lpxD}}{{Tab|Evolution|lpxD}}{{Tab|On One Page|lpxD}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Standard name
||
LpxD product
|-
!align=left {{table heading style}} |Synonyms
||
UDP-3-O-(3-hydroxymyristoyl)-glucosamine N-acyltransferase product<ref name='LIB:Riley_2006'/>, B0179 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, OmsA product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Ssc product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, OsmA product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, FirA product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, LpxD product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-MONO<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

|-
!align=left {{table heading style}} |EC number (for enzymes)
||
** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.- 2.3.1.-]<ref name='LIB:Riley_2006'/>
**[[kegg:ko00540.html|LPS biosynthesis]]

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19924.I46aa217dc177e&page=19924&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> {{Product_GO_help}}

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0008415_!_acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008415_!_acyltransferase_activity GO:0008415]
|
acyltransferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR001451
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008415_!_acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008415_!_acyltransferase_activity GO:0008415]
|
acyltransferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0012
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008610_!_lipid_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008610_!_lipid_biosynthetic_process GO:0008610]
|
lipid biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0444
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00523
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR007691
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0441
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR011004
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0808
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016747_!_transferase_activity,_transferring_groups_other_than_amino-acyl_groups]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016747_!_transferase_activity,_transferring_groups_other_than_amino-acyl_groups GO:0016747]
|
transferase activity, transferring groups other than amino-acyl groups
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00523
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016747_!_transferase_activity,_transferring_groups_other_than_amino-acyl_groups]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016747_!_transferase_activity,_transferring_groups_other_than_amino-acyl_groups GO:0016747]
|
transferase activity, transferring groups other than amino-acyl groups
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR007691
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0046677_!_response_to_antibiotic]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0046677_!_response_to_antibiotic GO:0046677]
|
response to antibiotic
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0046
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|

|

|

|
C
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting GO:0042280]
|
cell surface antigen activity, host-interacting
|

|

|

|
F
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0016410_!_N-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016410_!_N-acyltransferase_activity GO:0016410]
|
N-acyltransferase activity
|
[[PMID:3288280]]<ref name=PMID:3288280/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[[PMID:8366125]]<ref name=PMID:8366125/>
|
IMP: Inferred from Mutant Phenotype
|

|
P
|

|
complete
|-
|

|
[[Category:GO:0016410_!_N-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016410_!_N-acyltransferase_activity GO:0016410]
|
N-acyltransferase activity
|
[[PMID:8366125]]<ref name=PMID:8366125/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19924.E46aa217eddd1f&page=19924&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>


{|class = 'sortable' {{Prettytable}} id='152729'
|- {{table heading style}}
!Partner Type!!Partner!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19924.J46aa218ba9e10&page=19924&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' {{Prettytable}} id='102041'
|- {{table heading style}}
!Compartment!!Description!!Evidence!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19924.S46a7b2e2d615f&page=19924&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|{{Prettytable}}
|-
!{{table heading style}} |Sequence
|
[http://biocyc.org/ECOLI/sequence-aa?object=UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-MONO at EcoCyc]
<pre>MPSIRLADLA QQLDAELHGD GDIVITGVAS MQSAQTGHIT FMVNPKYREH LGLCQASAVV
MTQDDLPFAK SAALVVKNPY LTYARMAQIL DTTPQPAQNI APSAVIDATA KLGNNVSIGA
NAVIESGVEL GDNVIIGAGC FVGKNSKIGA GSRLWANVTI YHEIQIGQNC LIQSGTVVGA
DGFGYANDRG NWVKIPQIGR VIIGDRVEIG ACTTIDRGAL DDTIIGNGVI IDNQCQIAHN
VVIGDNTAVA GGVIMAGSLK IGRYCMIGGA SVINGHMEIC DKVTVTGMGM VMRPITEPGV
YSSGIPLQPN KVWRKTAALV MNIDDMSKRL KSLERKVNQQ D</pre>
|-
!{{table heading style}} |Mol. Wt
|
36.04 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |pI
|
6.48<ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |Extinction coefficient
|
26930 - 27805 (calc based on 7 Y, 3 W, and 7 C residues)
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19924.X46aa218d6786c&page=19924&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


{|class = 'sortable' {{Prettytable}} id='152731'
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19924.D46aa218dc0141&page=19924&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|class = 'sortable' {{Prettytable}} id='102043'
|- {{table heading style}}
!Structure Type!!Organism!!Accession!!Residues covered!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19924.Z46a7b2e475536&page=19924&pagename={{FULLPAGENAMEE}} edit table] || || || ||
|}

{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152732'
|- {{table_heading_style}}
!Resource type!!Source!!Notes/Reference
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19924.B46aa218e5ede9&page=19924&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152733'
|- {{table_heading_style}}
!Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-MONO EcoCyc:UDPHYDROXYMYRGLUCOSAMNACETYLTRANS-MONO]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P21645|UniProt:P21645]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF04613 PFAM:PF04613]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_414721 RefSeq:NP_414721]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=P21645 ModBase:P21645]
|

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19924.V46aa218ea8213&page=19924&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='102045'
|- {{table_heading_style}}
!Name!!URL!!Comments
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19924.J46a7b2e80fe27&page=19924&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0009245_!_lipid A biosynthetic process]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]]

lpxC:Gene Product(s)

2008-10-16T15:32:26Z

D-struck: Table edited by D-struck via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|lpxC}}{{Tab|Gene|lpxC}}{{Tab On|Gene Product(s)|lpxC}}{{Tab|Expression|lpxC}}{{Tab|Evolution|lpxC}}{{Tab|On One Page|lpxC}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Standard name
||
LpxC
|-
!align=left {{table heading style}} |Synonyms
||
UDP-3-O-acyl N-acetylglucosamine deacetylase<ref name='LIB:Riley_2006'/>, B0096<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, EnvA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AsmB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, LpxC<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
UDP-3-O-acyl N-acetylglucosamine deacetylase
|-
!align=left {{table heading style}} |EC number (for enzymes)
||
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.33 3.5.1.33]
*[[kegg:ko00540.html|LPS biosynthesis]]

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Y46aa1b7d35ea8&page=19678&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> {{Product_GO_help}}

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0008610_!_lipid_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008610_!_lipid_biosynthetic_process GO:0008610]
|
lipid biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0444
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00388
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004463
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR011334
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR015870
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00388
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004463
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR011334
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR015870
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0441
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016787_!_hydrolase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016787_!_hydrolase_activity GO:0016787]
|
hydrolase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0378
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|

|

|

|
C
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting GO:0042280]
|
cell surface antigen activity, host-interacting
|

|

|

|
F
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[[PMID:8530464]]<ref name=PMID:8530464/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[[PMID:8530464]]<ref name=PMID:8530464/>
|
IMP: Inferred from Mutant Phenotype
|

|
P
|

|
complete
|-
|

|
[[Category:GO:0019213_!_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0019213_!_deacetylase_activity GO:0019213]
|
deacetylase activity
|
[[PMID:3288280]]<ref name=PMID:3288280/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete

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|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.F46aa1b7e3152e&page=19678&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Partner Type!!Partner!!Notes
|-
|
Small Molecule
|
N-ethyl maleimide
|
Inhibits the activity of LpxC when added to membrane free extracts at 1mM concentration (see [[PMID:3288280]]<ref name=PMID:3288280/>).

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.M46aa1b8b10fd9&page=19678&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table] || ||
|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' {{Prettytable}} id='152114'
|- {{table heading style}}
!Compartment!!Description!!Evidence!!Notes
|-
|
cytoplasm
|

|

|
From EcoCyc<ref name='LIB:EcoCyc11.1'/>
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Q46aa1b8bb493e&page=19678&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|{{Prettytable}}
|-
!{{table heading style}} |Sequence
|
[http://biocyc.org/ECOLI/sequence-aa?object=UDPACYLGLCNACDEACETYL-MONOMER at EcoCyc]
<pre>MIKQRTLKRI VQATGVGLHT GKKVTLTLRP APANTGVIYR RTDLNPPVDF PADAKSVRDT
MLCTCLVNEH DVRISTVEHL NAALAGLGID NIVIEVNAPE IPIMDGSAAP FVYLLLDAGI
DELNCAKKFV RIKETVRVED GDKWAEFKPY NGFSLDFTID FNHPAIDSSN QRYAMNFSAD
AFMRQISRAR TFGFMRDIEY LQSRGLCLGG SFDCAIVVDD YRVLNEDGLR FEDEFVRHKM
LDAIGDLFMC GHNIIGAFTA YKSGHALNNK LLQAVLAKQE AWEYVTFQDD AELPLAFKAP
SAVLA</pre>
|-
!{{table heading style}} |Mol. Wt
|
33.96 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |pI
|
5.87<ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |Extinction coefficient
|
22920 - 23670 (calc based on 8 Y, 2 W, and 6 C residues)
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Y46aa1b8d263dc&page=19678&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


{|class = 'sortable' {{Prettytable}} id='152116'
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.R46aa1b8d7ecf1&page=19678&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|class = 'sortable' {{Prettytable}} id='100819'
|- {{table heading style}}
!Structure Type!!Organism!!Accession!!Residues covered!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.S46a7312b21a9a&page=19678&pagename={{FULLPAGENAMEE}} edit table] || || || ||
|}

{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152117'
|- {{table_heading_style}}
!Resource type!!Source!!Notes/Reference
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Z46aa1b8e1d901&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152118'
|- {{table_heading_style}}
!Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=UDPACYLGLCNACDEACETYL-MONOMER EcoCyc:UDPACYLGLCNACDEACETYL-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P0A725|UniProt:P0A725]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF03331 PFAM:PF03331]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_414638 RefSeq:NP_414638]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=P0A725 ModBase:P0A725]
|

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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Z46aa1b8e668c7&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='100820'
|- {{table_heading_style}}
!Name!!URL!!Comments
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.K46a7312e2333a&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0009245_!_lipid A biosynthetic process]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]][[Category:Location:cytoplasm]]

PMID:3288280

2008-10-16T15:30:08Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Anderson, MS, Robertson, AD, Macher, I and Raetz, CR''' (1988) Biosynthesis of lipid A in Escherichia coli: identification of UDP-3-O-[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine as a precursor of UDP-N2,O3-bis[(R)-3-hydroxymyristoyl]-alpha-D-glucosamine.''Biochemistry'' '''27''':1908-17

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || The lipid A disaccharide of the Escherichia coli envelope is synthesized from the two fatty acylated glucosamine derivatives UDP-N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D- glucosamine (UDP-2,3-diacyl-GlcN) and N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine 1-phosphate (2,3-diacyl-GlcN-1-P) [Ray, B. L., Painter, G., & Raetz, C. R. H. (1984) J. Biol. Chem. 259, 4852-4859]. We have previously shown that UDP-2,3-diacyl-GlcN is generated in extracts of E. coli by fatty acylation of UDP-GlcNAc, giving UDP-3-O-[(R)-3-hydroxymyristoyl]-GlcNAc as the first intermediate, which is rapidly converted to UDP-2,3-diacyl-GlcN [Anderson, M. S., Bulawa, C. E., & Raetz, C. R. H. (1985) J. Biol. Chem. 260, 15536-15541; Anderson, M. S., & Raetz, C. R. H. (1987) J. Biol. Chem. 262, 5159-5169]. We now demonstrate a novel enzyme in the cytoplasmic fraction of E. coli, capable of deacetylating UDP-3-O-[(R)-3-hydroxymyristoyl]-GlcNAc to form UDP-3-O-[(R)-3-hydroxymyristoyl]glucosamine. The covalent structure of the previously undescribed UDP-3-O-[(R)-3-hydroxymyristoyl] glucosamine intermediate was established by 1H NMR spectroscopy and fast atom bombardment mass spectrometry. This material can be made to accumulate in E. coli extracts upon incubation of UDP-3-O-[(R)-3- hydroxymyristoyl]-GlcNAc in the absence of the fatty acyl donor [(R)-3-hydroxymyristoyl]-acyl carrier protein. However, addition of the isolated deacetylation product [UDP-3-O-[(R)-3-hydroxymyristoyl] glucosamine] back to membrane-free extracts of E. coli in the presence of [(R)-3-hydroxymyristoyl]-acyl carrier protein results in rapid conversion of this compound into the more hydrophobic products UDP-2,3-diacyl-GlcN, 2,3-diacyl-GlcN-1-P, and O-[2-amino-2-deoxy-N2,O3- bis[(R)-3-hydroxytetradecanoyl]-beta-D-glucopyranosyl]-(1----6)-2-amino- 2-deoxy-N2,O3-bis[(R)-3-hydroxytetradecanoyl]-alpha-D- glucopyranose 1-phosphate (tetra-acyldisaccharide-1-P), demonstrating its competency as a precursor. In vitro incubations using [acetyl-3H]UDP-3-O-[(R)-3-hydroxymyristoyl]-GlcNAc confirmed release of the acetyl moiety in this system as acetate, not as some other acetyl derivative. The deacetylation reaction was inhibited by 1 mM N-ethylmaleimide, while the subsequent N-acylation reaction was not. Our observations provide strong evidence that UDP-3-O-[(R)-3-hydroxymyristoyl]glucosamine is a true intermediate in the biosynthesis of UDP-2,3-diacyl-GlcN and lipid A.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3288280 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxC
|

|
[[Category:GO:0019213_!_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0019213_!_deacetylase_activity GO:0019213]
|
deacetylase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
Demonstrated by the release of [3H]-acetic acid from [3H-acetyl]- UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc (pg. 1911 and Figure 8).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.Q47ab7f13d5151&page=132291&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

PMID:8530464

2008-10-16T15:24:06Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Young, K, Silver, LL, Bramhill, D, Cameron, P, Eveland, SS, Raetz, CR, Hyland, SA and Anderson, MS''' (1995) The envA permeability/cell division gene of Escherichia coli encodes the second enzyme of lipid A biosynthesis. UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase.''J. Biol. Chem.'' '''270''':30384-91

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || The envA gene of Escherichia coli has been shown previously to be essential for cell viability (Beall, B. and Lutkenhaus, J. (1987) J. Bacteriol. 169, 5408-5415), yet it encodes a protein of unknown function. Extracts of strains harboring the mutant envA1 allele display 3.5-18-fold reductions in UDP-3-O-acyl-N-acetylglucosamine deacetylase specific activity. The deacetylase is the second enzymatic step of lipid A biosynthesis. The structural gene coding for the deacetylase has not been assigned. In order to determine if the envA gene encodes the deacetylase, envA was cloned into an isopropyl-1-thio-beta-D-galactopyranoside-inducible T7-based expression system. Upon induction, a protein of the size of envA was highly overproduced, as judged by SDS-PAGE. Direct deacetylase assays of cell lysates revealed a concomitant approximately 5,000-fold overproduction of activity. Assays of the purified, overproduced EnvA protein demonstrated a further approximately 5-fold increase in specific activity. N-terminal amino acid sequencing of the purified protein showed that the first 20 amino acids matched the predicted envA nucleotide sequence. Contaminating species were present at less than 1% of the level of the EnvA protein. Thus, envA is the structural gene for UDP-3-O-acyl-GlcNAc deacetylase. Based on its function in lipid A biosynthesis, we propose the new designation lpxC for this gene.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8530464 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxC
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
Assayed using [alpha-32P]UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc as the substrate. Product was separated from the reactant by thin layer chromatography and quantified by scintillation counting. Substrate is not commercially available but its preparation is described.
|
complete
|-
|
LpxC
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
IMP: Inferred from Mutant Phenotype
|

|
P
|
Extracts prepared from strains with the lpxC101 (envA1) mutation have greatly reduced levels of UDP-3-O-acyl-N-acetylglucosamine deacetylase activity (Tables II and III). This activity is greatly enhanced by overexpression of the lpxC gene from a T7 promoter (Table III).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.N47ab7acb466e3&page=132103&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

lpxC:Gene Product(s)

2008-10-16T15:17:48Z

D-struck: Table edited by D-struck via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|lpxC}}{{Tab|Gene|lpxC}}{{Tab On|Gene Product(s)|lpxC}}{{Tab|Expression|lpxC}}{{Tab|Evolution|lpxC}}{{Tab|On One Page|lpxC}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Standard name
||
LpxC
|-
!align=left {{table heading style}} |Synonyms
||
UDP-3-O-acyl N-acetylglucosamine deacetylase<ref name='LIB:Riley_2006'/>, B0096<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, EnvA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AsmB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, LpxC<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
UDP-3-O-acyl N-acetylglucosamine deacetylase
|-
!align=left {{table heading style}} |EC number (for enzymes)
||
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.33 3.5.1.33]
*[[kegg:ko00540.html|LPS biosynthesis]]

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Y46aa1b7d35ea8&page=19678&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> {{Product_GO_help}}

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0008610_!_lipid_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008610_!_lipid_biosynthetic_process GO:0008610]
|
lipid biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0444
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00388
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004463
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR011334
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR015870
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00388
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004463
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR011334
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR015870
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0441
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016787_!_hydrolase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016787_!_hydrolase_activity GO:0016787]
|
hydrolase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0378
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|

|

|

|
C
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting GO:0042280]
|
cell surface antigen activity, host-interacting
|

|

|

|
F
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[[PMID:8530464]]<ref name=PMID:8530464/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[[PMID:8530464]]<ref name=PMID:8530464/>
|
IMP: Inferred from Mutant Phenotype
|

|
P
|

|
complete
|-
|

|
[[Category:GO:0019213_!_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0019213_!_deacetylase_activity GO:0019213]
|
deacetylase activity
|
[[PMID:3288280]]<ref name=PMID:3288280/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.F46aa1b7e3152e&page=19678&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>


{|class = 'sortable' {{Prettytable}} id='152113'
|- {{table heading style}}
!Partner Type!!Partner!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.M46aa1b8b10fd9&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' {{Prettytable}} id='152114'
|- {{table heading style}}
!Compartment!!Description!!Evidence!!Notes
|-
|
cytoplasm
|

|

|
From EcoCyc<ref name='LIB:EcoCyc11.1'/>
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Q46aa1b8bb493e&page=19678&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|{{Prettytable}}
|-
!{{table heading style}} |Sequence
|
[http://biocyc.org/ECOLI/sequence-aa?object=UDPACYLGLCNACDEACETYL-MONOMER at EcoCyc]
<pre>MIKQRTLKRI VQATGVGLHT GKKVTLTLRP APANTGVIYR RTDLNPPVDF PADAKSVRDT
MLCTCLVNEH DVRISTVEHL NAALAGLGID NIVIEVNAPE IPIMDGSAAP FVYLLLDAGI
DELNCAKKFV RIKETVRVED GDKWAEFKPY NGFSLDFTID FNHPAIDSSN QRYAMNFSAD
AFMRQISRAR TFGFMRDIEY LQSRGLCLGG SFDCAIVVDD YRVLNEDGLR FEDEFVRHKM
LDAIGDLFMC GHNIIGAFTA YKSGHALNNK LLQAVLAKQE AWEYVTFQDD AELPLAFKAP
SAVLA</pre>
|-
!{{table heading style}} |Mol. Wt
|
33.96 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |pI
|
5.87<ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |Extinction coefficient
|
22920 - 23670 (calc based on 8 Y, 2 W, and 6 C residues)
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Y46aa1b8d263dc&page=19678&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


{|class = 'sortable' {{Prettytable}} id='152116'
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.R46aa1b8d7ecf1&page=19678&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|class = 'sortable' {{Prettytable}} id='100819'
|- {{table heading style}}
!Structure Type!!Organism!!Accession!!Residues covered!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.S46a7312b21a9a&page=19678&pagename={{FULLPAGENAMEE}} edit table] || || || ||
|}

{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152117'
|- {{table_heading_style}}
!Resource type!!Source!!Notes/Reference
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Z46aa1b8e1d901&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152118'
|- {{table_heading_style}}
!Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=UDPACYLGLCNACDEACETYL-MONOMER EcoCyc:UDPACYLGLCNACDEACETYL-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P0A725|UniProt:P0A725]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF03331 PFAM:PF03331]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_414638 RefSeq:NP_414638]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=P0A725 ModBase:P0A725]
|

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Z46aa1b8e668c7&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='100820'
|- {{table_heading_style}}
!Name!!URL!!Comments
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.K46a7312e2333a&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0009245_!_lipid A biosynthetic process]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]][[Category:Location:cytoplasm]]

PMID:8530464

2008-10-12T17:17:16Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Young, K, Silver, LL, Bramhill, D, Cameron, P, Eveland, SS, Raetz, CR, Hyland, SA and Anderson, MS''' (1995) The envA permeability/cell division gene of Escherichia coli encodes the second enzyme of lipid A biosynthesis. UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase.''J. Biol. Chem.'' '''270''':30384-91

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || The envA gene of Escherichia coli has been shown previously to be essential for cell viability (Beall, B. and Lutkenhaus, J. (1987) J. Bacteriol. 169, 5408-5415), yet it encodes a protein of unknown function. Extracts of strains harboring the mutant envA1 allele display 3.5-18-fold reductions in UDP-3-O-acyl-N-acetylglucosamine deacetylase specific activity. The deacetylase is the second enzymatic step of lipid A biosynthesis. The structural gene coding for the deacetylase has not been assigned. In order to determine if the envA gene encodes the deacetylase, envA was cloned into an isopropyl-1-thio-beta-D-galactopyranoside-inducible T7-based expression system. Upon induction, a protein of the size of envA was highly overproduced, as judged by SDS-PAGE. Direct deacetylase assays of cell lysates revealed a concomitant approximately 5,000-fold overproduction of activity. Assays of the purified, overproduced EnvA protein demonstrated a further approximately 5-fold increase in specific activity. N-terminal amino acid sequencing of the purified protein showed that the first 20 amino acids matched the predicted envA nucleotide sequence. Contaminating species were present at less than 1% of the level of the EnvA protein. Thus, envA is the structural gene for UDP-3-O-acyl-GlcNAc deacetylase. Based on its function in lipid A biosynthesis, we propose the new designation lpxC for this gene.
</noinclude>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8530464 PubMed]
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxC
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
Assayed using [alpha-32P]UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc as the substrate. Product was separated from the reactant by thin layer chromatography and quantified by scintillation counting. Substrate is not commercially available but its preparation is described.
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.N47ab7acb466e3&page=132103&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}


==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==References==
{{RefHelp}}
<references/>

lpxC:Quickview

2008-10-10T17:27:30Z

D-struck: Table edited by D-struck via TableEdit

PMID:4887513

2008-10-10T17:25:08Z

D-struck: Fill PMID: Page!

{{RightTOC}}

 '''Normark, S, Boman, HG and Matsson, E''' (1969) Mutant of Escherichia coli with anomalous cell division and ability to decrease episomally and chromosomally mediated resistance to ampicillin and several other antibiotics.''J. Bacteriol.'' '''97''':1334-42

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>In a mutation experiment with a rough, ampicillin-resistant strain, we isolated two smooth mutants which were both sensitive to ampicillin and carried defects in the cell envelope. One of the strains (with the envA gene) is hindered in its completion of septa and forms chains of cells. The envA gene has been mapped to a position between leu and proB, at 2 to 4 min. The envA gene decreased the resistance mediated by both episomal and chromosomal genes for resistance to several antibiotics. During growth the envA mutant was characterized by abnormal ratios between viable count or cell count and optical density. The ratio between viable count and optical density was affected during shift-up and shift-down experiments. When compared to the parent strain, the envA mutant was found to be more resistant to ultraviolet irradiation on plates. Prestarvation for tryptophan had a protective effect against irradiation both on the parent strain and the envA mutant.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4887513 PubMed] 
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.147473.Z48ef8ff491d53&page=147473&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:4887513

2008-10-10T17:25:08Z

D-struck: New PMID: Page!

lpxC:Gene

2008-10-10T17:24:47Z

D-struck: Table edited by D-struck via TableEdit

lpxC:Gene Product(s)

2008-10-10T17:20:06Z

D-struck: Table edited by D-struck via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|lpxC}}{{Tab|Gene|lpxC}}{{Tab On|Gene Product(s)|lpxC}}{{Tab|Expression|lpxC}}{{Tab|Evolution|lpxC}}{{Tab|On One Page|lpxC}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Standard name
||
LpxC
|-
!align=left {{table heading style}} |Synonyms
||
UDP-3-O-acyl N-acetylglucosamine deacetylase<ref name='LIB:Riley_2006'/>, B0096<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, EnvA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AsmB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, LpxC<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
UDP-3-O-acyl N-acetylglucosamine deacetylase
|-
!align=left {{table heading style}} |EC number (for enzymes)
||
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.33 3.5.1.33]
*[[kegg:ko00540.html|LPS biosynthesis]]

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Y46aa1b7d35ea8&page=19678&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> {{Product_GO_help}}

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0008610_!_lipid_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008610_!_lipid_biosynthetic_process GO:0008610]
|
lipid biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0444
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00388
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004463
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR011334
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR015870
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00388
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004463
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR011334
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR015870
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0441
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016787_!_hydrolase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016787_!_hydrolase_activity GO:0016787]
|
hydrolase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0378
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|

|

|

|
C
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting GO:0042280]
|
cell surface antigen activity, host-interacting
|

|

|

|
F
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[[PMID:8530464]]<ref name=PMID:8530464/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.F46aa1b7e3152e&page=19678&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>


{|class = 'sortable' {{Prettytable}} id='152113'
|- {{table heading style}}
!Partner Type!!Partner!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.M46aa1b8b10fd9&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' {{Prettytable}} id='152114'
|- {{table heading style}}
!Compartment!!Description!!Evidence!!Notes
|-
|
cytoplasm
|

|

|
From EcoCyc<ref name='LIB:EcoCyc11.1'/>
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Q46aa1b8bb493e&page=19678&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|{{Prettytable}}
|-
!{{table heading style}} |Sequence
|
[http://biocyc.org/ECOLI/sequence-aa?object=UDPACYLGLCNACDEACETYL-MONOMER at EcoCyc]
<pre>MIKQRTLKRI VQATGVGLHT GKKVTLTLRP APANTGVIYR RTDLNPPVDF PADAKSVRDT
MLCTCLVNEH DVRISTVEHL NAALAGLGID NIVIEVNAPE IPIMDGSAAP FVYLLLDAGI
DELNCAKKFV RIKETVRVED GDKWAEFKPY NGFSLDFTID FNHPAIDSSN QRYAMNFSAD
AFMRQISRAR TFGFMRDIEY LQSRGLCLGG SFDCAIVVDD YRVLNEDGLR FEDEFVRHKM
LDAIGDLFMC GHNIIGAFTA YKSGHALNNK LLQAVLAKQE AWEYVTFQDD AELPLAFKAP
SAVLA</pre>
|-
!{{table heading style}} |Mol. Wt
|
33.96 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |pI
|
5.87<ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |Extinction coefficient
|
22920 - 23670 (calc based on 8 Y, 2 W, and 6 C residues)
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Y46aa1b8d263dc&page=19678&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


{|class = 'sortable' {{Prettytable}} id='152116'
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.R46aa1b8d7ecf1&page=19678&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|class = 'sortable' {{Prettytable}} id='100819'
|- {{table heading style}}
!Structure Type!!Organism!!Accession!!Residues covered!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.S46a7312b21a9a&page=19678&pagename={{FULLPAGENAMEE}} edit table] || || || ||
|}

{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152117'
|- {{table_heading_style}}
!Resource type!!Source!!Notes/Reference
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Z46aa1b8e1d901&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152118'
|- {{table_heading_style}}
!Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=UDPACYLGLCNACDEACETYL-MONOMER EcoCyc:UDPACYLGLCNACDEACETYL-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P0A725|UniProt:P0A725]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF03331 PFAM:PF03331]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_414638 RefSeq:NP_414638]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=P0A725 ModBase:P0A725]
|

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Z46aa1b8e668c7&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='100820'
|- {{table_heading_style}}
!Name!!URL!!Comments
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.K46a7312e2333a&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0009245_!_lipid A biosynthetic process]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]][[Category:Location:cytoplasm]]

lpxC:Gene Product(s)

2008-10-10T17:19:24Z

D-struck: Table edited by D-struck via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|lpxC}}{{Tab|Gene|lpxC}}{{Tab On|Gene Product(s)|lpxC}}{{Tab|Expression|lpxC}}{{Tab|Evolution|lpxC}}{{Tab|On One Page|lpxC}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Standard name
||
LpxC
|-
!align=left {{table heading style}} |Synonyms
||
UDP-3-O-acyl N-acetylglucosamine deacetylase<ref name='LIB:Riley_2006'/>, B0096<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, EnvA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, AsmB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, LpxC<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
UDP-3-O-acyl N-acetylglucosamine deacetylase
|-
!align=left {{table heading style}} |EC number (for enzymes)
||
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.33 3.5.1.33]
*[[kegg:ko00540.html|LPS biosynthesis]]

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Y46aa1b7d35ea8&page=19678&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> {{Product_GO_help}}

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0008610_!_lipid_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008610_!_lipid_biosynthetic_process GO:0008610]
|
lipid biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0444
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00388
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004463
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR011334
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR015870
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00388
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR004463
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR011334
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR015870
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0441
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016787_!_hydrolase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016787_!_hydrolase_activity GO:0016787]
|
hydrolase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0378
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|

|

|

|
C
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing
|-
|

|
[[Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting GO:0042280]
|
cell surface antigen activity, host-interacting
|

|

|

|
F
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008759_!_UDP-3-O-(3-hydroxymyristoyl)_N-acetylglucosamine_deacetylase_activity GO:0008759]
|
UDP-3-O-(3-hydroxymyristoyl) N-acetylglucosamine deacetylase activity
|
[[PMID:8530464]]<ref name=PMID:8530464/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|
[[PMID:1234567]]<ref name=PMID:1234567/>
|
IDA: Inferred from Direct Assay
|

|
C
|

|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.F46aa1b7e3152e&page=19678&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>


{|class = 'sortable' {{Prettytable}} id='152113'
|- {{table heading style}}
!Partner Type!!Partner!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.M46aa1b8b10fd9&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' {{Prettytable}} id='152114'
|- {{table heading style}}
!Compartment!!Description!!Evidence!!Notes
|-
|
cytoplasm
|

|

|
From EcoCyc<ref name='LIB:EcoCyc11.1'/>
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Q46aa1b8bb493e&page=19678&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|{{Prettytable}}
|-
!{{table heading style}} |Sequence
|
[http://biocyc.org/ECOLI/sequence-aa?object=UDPACYLGLCNACDEACETYL-MONOMER at EcoCyc]
<pre>MIKQRTLKRI VQATGVGLHT GKKVTLTLRP APANTGVIYR RTDLNPPVDF PADAKSVRDT
MLCTCLVNEH DVRISTVEHL NAALAGLGID NIVIEVNAPE IPIMDGSAAP FVYLLLDAGI
DELNCAKKFV RIKETVRVED GDKWAEFKPY NGFSLDFTID FNHPAIDSSN QRYAMNFSAD
AFMRQISRAR TFGFMRDIEY LQSRGLCLGG SFDCAIVVDD YRVLNEDGLR FEDEFVRHKM
LDAIGDLFMC GHNIIGAFTA YKSGHALNNK LLQAVLAKQE AWEYVTFQDD AELPLAFKAP
SAVLA</pre>
|-
!{{table heading style}} |Mol. Wt
|
33.96 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |pI
|
5.87<ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |Extinction coefficient
|
22920 - 23670 (calc based on 8 Y, 2 W, and 6 C residues)
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Y46aa1b8d263dc&page=19678&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


{|class = 'sortable' {{Prettytable}} id='152116'
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.R46aa1b8d7ecf1&page=19678&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|class = 'sortable' {{Prettytable}} id='100819'
|- {{table heading style}}
!Structure Type!!Organism!!Accession!!Residues covered!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.S46a7312b21a9a&page=19678&pagename={{FULLPAGENAMEE}} edit table] || || || ||
|}

{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152117'
|- {{table_heading_style}}
!Resource type!!Source!!Notes/Reference
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Z46aa1b8e1d901&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152118'
|- {{table_heading_style}}
!Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=UDPACYLGLCNACDEACETYL-MONOMER EcoCyc:UDPACYLGLCNACDEACETYL-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P0A725|UniProt:P0A725]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=PF03331 PFAM:PF03331]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_414638 RefSeq:NP_414638]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=P0A725 ModBase:P0A725]
|

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.Z46aa1b8e668c7&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='100820'
|- {{table_heading_style}}
!Name!!URL!!Comments
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19678.K46a7312e2333a&page=19678&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0009245_!_lipid A biosynthetic process]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]][[Category:Location:cytoplasm]]

lpxA:Gene Product(s)

2008-10-10T16:03:41Z

D-struck: Table edited by D-struck via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|lpxA}}{{Tab|Gene|lpxA}}{{Tab On|Gene Product(s)|lpxA}}{{Tab|Expression|lpxA}}{{Tab|Evolution|lpxA}}{{Tab|On One Page|lpxA}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Standard name
||
LpxA
|-
!align=left {{table heading style}} |Synonyms
||
UDP-N-acetylglucosamine acyltransferase product<ref name='LIB:Riley_2006'/>, B0181 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, LpxA product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
LpxA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;

Component of ; [[:Category:Complex:UDP-N-acetylglucosamine acyltransferase|UDP-N-acetylglucosamine acyltransferase]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

|-
!align=left {{table heading style}} |EC number (for enzymes)
||
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.129 2.3.1.129]<ref name='LIB:Riley_2006'/>
*[[kegg:ko00540.html|LPS biosynthesis]]

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.I46aa21a4845af&page=19929&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big> {{Product_GO_help}}

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0963
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008415_!_acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008415_!_acyltransferase_activity GO:0008415]
|
acyltransferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR001451
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008415_!_acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008415_!_acyltransferase_activity GO:0008415]
|
acyltransferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0012
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008610_!_lipid_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008610_!_lipid_biosynthetic_process GO:0008610]
|
lipid biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR010137
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008610_!_lipid_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008610_!_lipid_biosynthetic_process GO:0008610]
|
lipid biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0444
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity GO:0008780]
|
acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine O-acyltransferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00387
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity GO:0008780]
|
acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine O-acyltransferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR010137
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity GO:0008780]
|
acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine O-acyltransferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000003 GO_REF:0000003]
|
IEA: Inferred from Electronic Annotation
|
EC:2.3.1.129
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000020 GO_REF:0000020]
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00387
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid A biosynthetic process
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0441
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000002 GO_REF:0000002]
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR011004
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
[http://www.geneontology.org/cgi-bin/references.cgi#GO_REF:0000004 GO_REF:0000004]
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0808
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|

|
[[Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting GO:0042280]
|
cell surface antigen activity, host-interacting
|

|

|

|
F
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity GO:0008780]
|
acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine O-acyltransferase activity
|
[[PMID:3549716]]<ref name=PMID:3549716/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete
|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|
[[PMID:3549716]]<ref name=PMID:3549716/>
|
IDA: Inferred from Direct Assay
|

|
C
|

|
complete
|-
|

|
[[Category:GO:0008415_!_acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008415_!_acyltransferase_activity GO:0008415]
|
acyltransferase activity
|
[[PMID:3549716]]<ref name=PMID:3549716/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.C46aa21a5d7ff6&page=19929&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}


<big>'''Interactions'''</big>


{|class = 'sortable' {{Prettytable}} id='152743'
|- {{table heading style}}
!Partner Type!!Partner!!Notes
|-
|
Protein
|
Subunits of [[:Category:Complex:UDP-N-acetylglucosamine acyltransferase|UDP-N-acetylglucosamine acyltransferase]]
|
could be indirect
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.W46aa21b2b6390&page=19929&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' {{Prettytable}} id='152744'
|- {{table heading style}}
!Compartment!!Description!!Evidence!!Notes
|-
|
cytoplasm
|

|

|
From EcoCyc<ref name='LIB:EcoCyc11.1'/>
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.I46aa21b3bec38&page=19929&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|{{Prettytable}}
|-
!{{table heading style}} |Sequence
|
[http://biocyc.org/ECOLI/sequence-aa?object=UDPNACETYLGLUCOSAMACYLTRANS-MONOMER at EcoCyc]
<pre>MIDKSAFVHP TAIVEEGASI GANAHIGPFC IVGPHVEIGE GTVLKSHVVV NGHTKIGRDN
EIYQFASIGE VNQDLKYAGE PTRVEIGDRN RIRESVTIHR GTVQGGGLTK VGSDNLLMIN
AHIAHDCTVG NRCILANNAT LAGHVSVDDF AIIGGMTAVH QFCIIGAHVM VGGCSGVAQD
VPPYVIAQGN HATPFGVNIE GLKRRGFSRE AITAIRNAYK LIYRSGKTLD EVKPEIAELA
ETYPEVKAFT DFFARSTRGL IR</pre>
|-
!{{table heading style}} |Mol. Wt
|
28.08 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |pI
|
6.83<ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |Extinction coefficient
|
8940 - 9565 (calc based on 6 Y, 0 W, and 5 C residues)
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.S46aa21b52e9f3&page=19929&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


{|class = 'sortable' {{Prettytable}} id='152746'
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.V46aa21b586dac&page=19929&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|class = 'sortable' {{Prettytable}} id='152747'
|- {{table heading style}}
!Structure Type!!Organism!!Accession!!Residues covered!!Notes
|-
|
X-RAY DIFFRACTION

|
Escherichia coli

|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1LXA 1LXA] UDP N-ACETYLGLUCOSAMINE ACYLTRANSFERASE
|

|

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.U46aa21b5cf868&page=19929&pagename={{FULLPAGENAMEE}} edit table] || || || ||
|}

{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152748'
|- {{table_heading_style}}
!Resource type!!Source!!Notes/Reference
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.P46aa21b68b996&page=19929&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152749'
|- {{table_heading_style}}
!Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=UDPNACETYLGLUCOSAMACYLTRANS-MONOMER EcoCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P0A722|UniProt:P0A722]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=7 of PF00132 PFAM:7 of PF00132]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_414723 RefSeq:NP_414723]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=P0A722 ModBase:P0A722]
|

|-
|
[http://www.rcsb.org/pdb PDB] ([[PDB|EcoliWiki Page]])
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1LXA PDB:1LXA]
|

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.B46aa21b6d4e74&page=19929&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='102060'
|- {{table_heading_style}}
!Name!!URL!!Comments
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.D46a7b311cd9f7&page=19929&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0009245_!_lipid A biosynthetic process]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]][[Category:Complex:UDP-N-acetylglucosamine acyltransferase]][[Category:Location:cytoplasm]][[Category:Products with structures in PDB]]

PMID:3549716

2008-10-10T16:01:18Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Anderson, MS and Raetz, CR''' (1987) Biosynthesis of lipid A precursors in Escherichia coli. A cytoplasmic acyltransferase that converts UDP-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine.''J. Biol. Chem.'' '''262''':5159-69

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Preliminary studies from our laboratory have suggested the existence of a novel set of fatty acyltransferases in extracts of Escherichia coli that attach two R-3-hydroxymyristoyl moieties to UDP-GlcNAc (Anderson, M.S., Bulawa, C.E., and Raetz, C.R.H. (1985) J. Biol. Chem. 260, 15536-15541). The resulting "glucosamine-derived" phospholipids appear to be crucial precursors for the biosynthesis of the lipid A component of lipopolysaccharide. We now describe an assay and a 1000-fold purification of the first enzyme in this pathway, which catalyzes the reaction: UDP-GlcNAc + R-3-hydroxymyristoyl-acyl carrier protein----UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc + acyl carrier protein. The covalent structure of the monoacylated UDP-GlcNAc product was established by fast atom bombardment mass spectrometry and 1H-NMR spectroscopy. The UDP-GlcNAc acyltransferase has a strict requirement for R-3-hydroxymyristoyl-acyl carrier protein, since R-3-hydroxymyristoyl coenzyme A and myristoyl-acyl carrier protein are not substrates. Of various NDP-GlcNAc preparations examined, only the uridine and thymidine derivatives were utilized to a significant extent. When the product of the reaction (UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc) was isolated and reincubated with crude E. coli extracts, it was rapidly converted to more hydrophobic products in the presence of R-3-hydroxymyristoyl-acyl carrier protein. We propose that the addition of an R-3-hydroxymyristoyl residue to the 3 position of the GlcNAc moiety of UDP-GlcNAc is the first committed step in lipid A biosynthesis and that UDP-GlcNAc is situated at a biosynthetic branchpoint in E. coli leading either to lipid A or to peptidoglycan.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3549716 PubMed] 
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxA
|

|
[[Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity GO:0008780]
|
acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine O-acyltransferase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
Assayed using [-32P]UDP-GlcNAc and R-3-hydroxymyristoyl acyl carrier protein as substrates. Product was separated from reactants by thin layer chromatography and quantified by scintillation counting (pg. 5160). Substrates are not commercially available but their preparation is described.
|
complete
|-
|
LpxA
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|
IDA: Inferred from Direct Assay
|

|
C
|
Activity found exclusively in the soluble fraction after centrifugation of a cell lysate at 150,000g for 90 minutes (pg. 5161).
|
complete
|-
|
LpxA
|

|
[[Category:GO:0008415_!_acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008415_!_acyltransferase_activity GO:0008415]
|
acyltransferase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
The enzyme is highly specific for R-3-hydroxyacyl-ACP and does not use non-hydroxylated derivatives. Hydroxyacyl-CoA is not a substrate (Table VI).
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.147442.V48e4e839be3ee&page=147442&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:3549716

2008-10-10T15:47:20Z

D-struck: Table edited by D-struck via TableEdit

{{RightTOC}}

 '''Anderson, MS and Raetz, CR''' (1987) Biosynthesis of lipid A precursors in Escherichia coli. A cytoplasmic acyltransferase that converts UDP-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine.''J. Biol. Chem.'' '''262''':5159-69

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Preliminary studies from our laboratory have suggested the existence of a novel set of fatty acyltransferases in extracts of Escherichia coli that attach two R-3-hydroxymyristoyl moieties to UDP-GlcNAc (Anderson, M.S., Bulawa, C.E., and Raetz, C.R.H. (1985) J. Biol. Chem. 260, 15536-15541). The resulting "glucosamine-derived" phospholipids appear to be crucial precursors for the biosynthesis of the lipid A component of lipopolysaccharide. We now describe an assay and a 1000-fold purification of the first enzyme in this pathway, which catalyzes the reaction: UDP-GlcNAc + R-3-hydroxymyristoyl-acyl carrier protein----UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc + acyl carrier protein. The covalent structure of the monoacylated UDP-GlcNAc product was established by fast atom bombardment mass spectrometry and 1H-NMR spectroscopy. The UDP-GlcNAc acyltransferase has a strict requirement for R-3-hydroxymyristoyl-acyl carrier protein, since R-3-hydroxymyristoyl coenzyme A and myristoyl-acyl carrier protein are not substrates. Of various NDP-GlcNAc preparations examined, only the uridine and thymidine derivatives were utilized to a significant extent. When the product of the reaction (UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc) was isolated and reincubated with crude E. coli extracts, it was rapidly converted to more hydrophobic products in the presence of R-3-hydroxymyristoyl-acyl carrier protein. We propose that the addition of an R-3-hydroxymyristoyl residue to the 3 position of the GlcNAc moiety of UDP-GlcNAc is the first committed step in lipid A biosynthesis and that UDP-GlcNAc is situated at a biosynthetic branchpoint in E. coli leading either to lipid A or to peptidoglycan.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3549716 PubMed] 
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
LpxA
|

|
[[Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity GO:0008780]
|
acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine O-acyltransferase activity
|
IDA: Inferred from Direct Assay
|

|
F
|
Assayed using [-32P]UDP-GlcNAc and R-3-hydroxymyristoyl acyl carrier protein as substrates. Product was separated from reactants by thin layer chromatography and quantified by scintillation counting. Substrates are not commercially available but their preparation is described.
|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.147442.V48e4e839be3ee&page=147442&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:3549716

2008-10-02T15:26:49Z

D-struck: Fill PMID: Page!

{{RightTOC}}

 '''Anderson, MS and Raetz, CR''' (1987) Biosynthesis of lipid A precursors in Escherichia coli. A cytoplasmic acyltransferase that converts UDP-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine.''J. Biol. Chem.'' '''262''':5159-69

{|{{Prettytable}} align = 'center' width= '70%'
|-
|{{TableHeadingColor}} |'''Abstract''' || <protect>Preliminary studies from our laboratory have suggested the existence of a novel set of fatty acyltransferases in extracts of Escherichia coli that attach two R-3-hydroxymyristoyl moieties to UDP-GlcNAc (Anderson, M.S., Bulawa, C.E., and Raetz, C.R.H. (1985) J. Biol. Chem. 260, 15536-15541). The resulting "glucosamine-derived" phospholipids appear to be crucial precursors for the biosynthesis of the lipid A component of lipopolysaccharide. We now describe an assay and a 1000-fold purification of the first enzyme in this pathway, which catalyzes the reaction: UDP-GlcNAc + R-3-hydroxymyristoyl-acyl carrier protein----UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc + acyl carrier protein. The covalent structure of the monoacylated UDP-GlcNAc product was established by fast atom bombardment mass spectrometry and 1H-NMR spectroscopy. The UDP-GlcNAc acyltransferase has a strict requirement for R-3-hydroxymyristoyl-acyl carrier protein, since R-3-hydroxymyristoyl coenzyme A and myristoyl-acyl carrier protein are not substrates. Of various NDP-GlcNAc preparations examined, only the uridine and thymidine derivatives were utilized to a significant extent. When the product of the reaction (UDP-3-O-(R-3-hydroxymyristoyl)-GlcNAc) was isolated and reincubated with crude E. coli extracts, it was rapidly converted to more hydrophobic products in the presence of R-3-hydroxymyristoyl-acyl carrier protein. We propose that the addition of an R-3-hydroxymyristoyl residue to the 3 position of the GlcNAc moiety of UDP-GlcNAc is the first committed step in lipid A biosynthesis and that UDP-GlcNAc is situated at a biosynthetic branchpoint in E. coli leading either to lipid A or to peptidoglycan.</protect>
|-
|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3549716 PubMed] 
|}

==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.147442.V48e4e839be3ee&page=147442&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:3549716

2008-10-02T15:26:49Z

D-struck: New PMID: Page!

lpxA:Gene Product(s)

2008-10-02T15:26:37Z

D-struck: Table edited by D-struck via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|lpxA}}{{Tab|Gene|lpxA}}{{Tab On|Gene Product(s)|lpxA}}{{Tab|Expression|lpxA}}{{Tab|Evolution|lpxA}}{{Tab|On One Page|lpxA}}{{TabsBottom}}
{|
|-
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Function |Function]]
|{{Sub}}[[#Interactions |Interactions]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude>
==Nomenclature==


{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"

|-
!align=left {{table heading style}} |Standard name
||
LpxA
|-
!align=left {{table heading style}} |Synonyms
||
UDP-N-acetylglucosamine acyltransferase product<ref name='LIB:Riley_2006'/>, B0181 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, LpxA product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>
|-
!align=left {{table heading style}} |Product description
||
LpxA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;

Component of ; [[:Category:Complex:UDP-N-acetylglucosamine acyltransferase|UDP-N-acetylglucosamine acyltransferase]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>

|-
!align=left {{table heading style}} |EC number (for enzymes)
||
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.129 2.3.1.129]<ref name='LIB:Riley_2006'/>

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.I46aa21a4845af&page=19929&pagename={{FULLPAGENAMEE}}&type=1&template=Product_nomenclature_table edit table] ||
|}

{{Product_nomenclature_help}}
<protect></protect>
===Notes===

==Function==
{{BacktoTop}}
<protect>
<big>'''Gene Ontology'''</big>


{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid_A_biosynthesis
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000004 GO_REF:0000004] 
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0441
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase_activity
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000002 GO_REF:0000002] 
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR011004
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase_activity
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000004 GO_REF:0000004] 
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0808
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0008415_!_acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008415_!_acyltransferase_activity GO:0008415]
|
acyltransferase_activity
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000002 GO_REF:0000002] 
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR001451
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0008415_!_acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008415_!_acyltransferase_activity GO:0008415]
|
acyltransferase_activity
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000004 GO_REF:0000004] 
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0012
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0008610_!_lipid_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008610_!_lipid_biosynthetic_process GO:0008610]
|
lipid_biosynthesis
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000002 GO_REF:0000002] 
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR010137
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0008610_!_lipid_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008610_!_lipid_biosynthetic_process GO:0008610]
|
lipid_biosynthesis
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000004 GO_REF:0000004] 
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0444
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity GO:0008780]
|
acyl-[acyl-carrier_protein]-UDP-N-acetylglucosamine_O-acyltransferase_activity
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000020 GO_REF:0000020] 
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00387
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity GO:0008780]
|
acyl-[acyl-carrier_protein]-UDP-N-acetylglucosamine_O-acyltransferase_activity
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000002 GO_REF:0000002] 
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR010137
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity GO:0008780]
|
acyl-[acyl-carrier_protein]-UDP-N-acetylglucosamine_O-acyltransferase_activity
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000003 GO_REF:0000003] 
|
IEA: Inferred from Electronic Annotation
|
EC:2.3.1.129
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000004 GO_REF:0000004] 
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0963
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0009245_!_lipid_A_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009245_!_lipid_A_biosynthetic_process GO:0009245]
|
lipid_A_biosynthesis
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000020 GO_REF:0000020] 
|
IEA: Inferred from Electronic Annotation
|
HAMAP:MF_00387
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|

|-
|

|
[[Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting GO:0042280]
|
cell surface antigen activity, host-interacting
|

|

|

|
F
|
Seeded from Riley et al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing
|-
|

|
[[Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008780_!_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine_O-acyltransferase_activity GO:0008780]
|
acyl-(acyl-carrier-protein)-UDP-N-acetylglucosamine O-acyltransferase activity
|
[[PMID:3549716]]<ref name=PMID:3549716/>
|
IDA: Inferred from Direct Assay
|

|
F
|

|
complete

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.C46aa21a5d7ff6&page=19929&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table] || || || || || || || ||
|}

{{Product_GO_help}}

<big>'''Interactions'''</big>


{|class = 'sortable' {{Prettytable}} id='152743'
|- {{table heading style}}
!Partner Type!!Partner!!Notes
|-
|
Protein
|
Subunits of [[:Category:Complex:UDP-N-acetylglucosamine acyltransferase|UDP-N-acetylglucosamine acyltransferase]]
|
could be indirect
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.W46aa21b2b6390&page=19929&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_interactions_help}}
</protect>
===Notes===

==Localization==


{|class = 'sortable' {{Prettytable}} id='152744'
|- {{table heading style}}
!Compartment!!Description!!Evidence!!Notes
|-
|
cytoplasm
|

|

|
From EcoCyc<ref name='LIB:EcoCyc11.1'/>
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.I46aa21b3bec38&page=19929&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_localization_help}}
<protect></protect>

===Notes===

==Structure and Physical Properties==
{{BacktoTop}}
<protect>
<big>'''Physical Properties'''</big>


{|{{Prettytable}}
|-
!{{table heading style}} |Sequence
|
[http://biocyc.org/ECOLI/sequence-aa?object=UDPNACETYLGLUCOSAMACYLTRANS-MONOMER at EcoCyc]
<pre>MIDKSAFVHP TAIVEEGASI GANAHIGPFC IVGPHVEIGE GTVLKSHVVV NGHTKIGRDN
EIYQFASIGE VNQDLKYAGE PTRVEIGDRN RIRESVTIHR GTVQGGGLTK VGSDNLLMIN
AHIAHDCTVG NRCILANNAT LAGHVSVDDF AIIGGMTAVH QFCIIGAHVM VGGCSGVAQD
VPPYVIAQGN HATPFGVNIE GLKRRGFSRE AITAIRNAYK LIYRSGKTLD EVKPEIAELA
ETYPEVKAFT DFFARSTRGL IR</pre>
|-
!{{table heading style}} |Mol. Wt
|
28.08 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |pI
|
6.83<ref name='LIB:EcoCyc10.6'/>
|-
!{{table heading style}} |Extinction coefficient
|
8940 - 9565 (calc based on 6 Y, 0 W, and 5 C residues)
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.S46aa21b52e9f3&page=19929&pagename={{FULLPAGENAMEE}} edit table]
|}

{{Product_physical_properties_help}}

<big>'''Domains/Motifs/Modification Sites'''</big>


{|class = 'sortable' {{Prettytable}} id='152746'
|- {{table heading style}}
!Type!!Residues!!Description!!Notes
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.V46aa21b586dac&page=19929&pagename={{FULLPAGENAMEE}} edit table] || || ||
|}

{{Product_domains_motifs_help}}

<big>'''Structure'''</big>


{|class = 'sortable' {{Prettytable}} id='152747'
|- {{table heading style}}
!Structure Type!!Organism!!Accession!!Residues covered!!Notes
|-
|
X-RAY DIFFRACTION

|
Escherichia coli

|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1LXA 1LXA] UDP N-ACETYLGLUCOSAMINE ACYLTRANSFERASE
|

|

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.U46aa21b5cf868&page=19929&pagename={{FULLPAGENAMEE}} edit table] || || || ||
|}

{{Product_structure_help}}
</protect>
===Notes===

==Gene Product Resources==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152748'
|- {{table_heading_style}}
!Resource type!!Source!!Notes/Reference
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.P46aa21b68b996&page=19929&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_resources_help}}
<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='152749'
|- {{table_heading_style}}
!Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=UDPNACETYLGLUCOSAMACYLTRANS-MONOMER EcoCyc:UDPNACETYLGLUCOSAMACYLTRANS-MONOMER]
|

|-
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
[[uniprot:P0A722|UniProt:P0A722]]
|

|-
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
|
[http://pfam.sanger.ac.uk/family?acc=7 of PF00132 PFAM:7 of PF00132]
|

|-
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_414723 RefSeq:NP_414723]
|

|-
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
|
[http://salilab.org/modbase/searchbyid?databaseID=P0A722 ModBase:P0A722]
|

|-
|
[http://www.rcsb.org/pdb PDB] ([[PDB|EcoliWiki Page]])
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1LXA PDB:1LXA]
|

|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.B46aa21b6d4e74&page=19929&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Product_accessions_help}}
<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{|class = 'sortable' {{Prettytable}} id='102060'
|- {{table_heading_style}}
!Name!!URL!!Comments
|-class='sortbottom'
|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.19929.D46a7b311cd9f7&page=19929&pagename={{FULLPAGENAMEE}} edit table] || ||
|}

{{Links_help}}

<noinclude>
==References==
{{BacktoTop}}
{{RefHelp}}
<references/>
__NOTOC__
</noinclude>
===Categories===
{{BacktoTop}}
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0009245_!_lipid A biosynthetic process]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]][[Category:Complex:UDP-N-acetylglucosamine acyltransferase]][[Category:Location:cytoplasm]][[Category:Products with structures in PDB]]