EcoliWiki - User contributions [en]

PMID:18248777

2012-08-05T17:02:29Z

Chrischen: Fill PMID: Page!

{{RightTOC}}


{| id="F501ea72477697" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Croteau, DL, DellaVecchia, MJ, Perera, L and Van Houten, B''' (2008) Cooperative damage recognition by UvrA and UvrB: identification of UvrA residues that mediate DNA binding.''DNA Repair (Amst.)'' '''7''':392-404
|-
!align=left |Abstract
||
Nucleotide excision repair (NER) is responsible for the recognition and removal of numerous structurally unrelated DNA lesions. In prokaryotes, the proteins UvrA, UvrB and UvrC orchestrate the recognition and excision of aberrant lesions from DNA. Despite the progress we have made in understanding the NER pathway, it remains unclear how the UvrA dimer interacts with DNA to facilitate DNA damage recognition. The purpose of this study was to define amino acid residues in UvrA that provide binding energy to DNA. Based on conservation among approximately 300 UvrA sequences and 3D-modeling, two positively charged residues, Lys680 and Arg691, were predicted to be important for DNA binding. Mutagenesis and biochemical analysis of Bacillus caldontenax UvrA variant proteins containing site directed mutations at these residues demonstrate that Lys680 and Arg691 make a significant contribution toward the DNA binding affinity of UvrA. Replacing these side chains with alanine or negatively charged residues decreased UvrA binding 3-37-fold. Survival studies indicated that these mutant proteins complemented a WP2 uvrA(-) strain of bacteria 10-100% of WT UvrA levels. Further analysis by DNase I footprinting of the double UvrA mutant revealed that the UvrA DNA binding defects caused a slower rate of transfer of DNA to UvrB. Consequently, the mutants initiated the oligonucleotide incision assay nearly as well as WT UvrA thus explaining the observed mild phenotype in the survival assay. Based on our findings we propose a model of how UvrA binds to DNA.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18248777 PubMed]
Online version:[http://dx.doi.org/10.1016/j.dnarep.2007.11.013 10.1016/j.dnarep.2007.11.013]
|-
!align=left |Keywords
||
Adenosine Triphosphatases; Adenosine Triphosphate; DNA Damage; DNA Footprinting; DNA Helicases; DNA Repair; DNA, Bacterial; DNA-Binding Proteins; Electrophoretic Mobility Shift Assay; Escherichia coli; Escherichia coli Proteins; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation

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|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="C501ea72521e04" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182648.C501ea72521e04&page=182648&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:18248777

2012-08-05T17:02:28Z

Chrischen: New PMID: Page!

recQ:Gene

2012-04-25T23:30:24Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|recQ}}{{Tab On|Gene|recQ}}{{Tab|Gene Product(s)|recQ}}{{Tab|Expression|recQ}}{{Tab|Evolution|recQ}}{{Tab|On One Page|recQ}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="O46a9ffd677cf5" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''recQ''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Recombination
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK3816, b3822, JW5855''<ref name='LIB:Riley_2006'/>, JW5855''

|- class="tableEdit_footer"
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|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
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<protect>

{{Gene_location_help}}
{| id="B46a9ffd705fc0" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
86.3 minutes 
|
MG1655: 4003887..4005716 <gbrowseImage>
name=NC_000913:4003887..4005716
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10833 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4002887|4006716|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4003887|4005716|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10833 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 3970285..3972120 <gbrowseImage>
name=NC_012967:3970285..3972120
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|3970285|3972120|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|3970285|3972120|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 3893556..3895385 <gbrowseImage>
name=NC_012759:3893556..3895385
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|3893556|3895385|GBrowse (BW2952)}}
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{{GBrowseFastaLink|BW2952|NC_012759|3893556|3895385|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 3630817..3628988 <gbrowseImage>
name=AC_000091:3628988..3630817
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|3627988|3631817|GBrowse(W3110)}}
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{{GBrowseFastaLink|W3110|AC_000091|3628988|3630817|FASTA Dump}}
|-
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[[DH10B]]
|

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DH10B: 4102807..4104636 <gbrowseImage>
name=NC_010473:4102807..4104636
source=DH10B
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|DH10B|NC_010473|4099149|4108294|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|4099149|4108294|FASTA Dump}}

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</protect>
===Notes===

==Sequence Features==
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{{Gene_sequence_features_help}}
{| id="H46a9ffd7bf015" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
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[[MG1655]]
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4003890
|
Edman degradation
|
PMID:2164680 
|

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|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
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{{Gene_alleles_help}}
{| {{Prettytable}} id="Y46a9ffd815243" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
ΔrecQ (Keio:JW5855)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
|

|-
|
''recQ1803::Tn3''
|

|

|

|

|
PMID:2993821
|
[[cgscmutation:65458|CGSC:65458]]
|

|-
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''recQ1'' (HN15)
|

|

|
Resistant to
|
Mutation causes partial resistance to thymineless death (TLD), figure 1.
|
PMID:6381965
|

|
Parent Strain: AB2497

Mutant previously designated ''tld-1''
|-
|
''recQ1'' (HN15)
|

|

|
Sensitivity to
|
Mutation causes increased UV sensitivity in a ''recBC sbcB'' background. (Data not shown)
|
PMID:6381965
|

|
Parent Strain: AB2497

Mutant previously designated ''tld-1''

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<protect></protect>
===Notes===

==Genetic Interactions==
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<protect>
{{Genetic_interaction_help}}
{| id="G4e04c230ba56c" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18207.G4e04c230ba56c&page=18207&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
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</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="X46a9ffd8cdf6e" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW5855
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Plasmid clone
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGCGCAGGCGGAAGTGTTGAA

Primer 2:CCtTCTTCGTCATCGCCATCAAC
|-
|
[[:Category:Kohara_Phage:1C10|1C10]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=551 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:7G3|7G3]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=552 Genobase]
|
PMID:3038334
|-
|
[[ilvD:Gene|ilvD500::Tn10]]
|
Linked marker
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CAG18431 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40338 CGSC7462]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 9% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:ilv500::Tn10
|-
|
metEo-3079::Tn10
|
Linked marker
|
CAG18491 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40350 CGSC7464]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 85% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.

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|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="R46a9ffd979bdf" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10833 ECOCYC:EG10833]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG10833 EcoGene:EG10833]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948318 GeneID:948318]
|
Escherichia coli str. K-12 substr. MG1655

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|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="D46a719bcc09a9" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18207.D46a719bcc09a9&page=18207&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:rec genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

PMID:6381965

2012-04-25T23:29:40Z

Chrischen: /* Significance {{HelpIcon|Help:Literature}} */

{{RightTOC}}


{| id="Z4f988805572d2" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Nakayama, H, Nakayama, K, Nakayama, R, Irino, N, Nakayama, Y and Hanawalt, PC''' (1984) Isolation and genetic characterization of a thymineless death-resistant mutant of Escherichia coli K12: identification of a new mutation (recQ1) that blocks the RecF recombination pathway.''Mol. Gen. Genet.'' '''195''':474-80
|-
!align=left |Abstract
||
An Escherichia coli K12 mutant resistant to thymineless death (TLD) was isolated, and its genetic analysis led us to identify a new mutation (recQ1) located between corA and metE on the standard linkage map. The mutation was found to result in increased sensitivity to ultraviolet light and deficiency in conjugational recombination when placed in the recBC sbcB background, indicating that it blocked the RecF pathway of recombination. It seemed likely that this mutation is also capable of causing partial resistance to TLD, but we reserve the possibility of a separate mutation closely linked to recQ1 giving rise to this phenotype. The original mutant was shown to carry an additional mutation probably in the vicinity of the uhp locus, which was also required for the full TLD resistance of the mutant to be expressed.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6381965 PubMed]

|-
!align=left |Keywords
||
Chromosome Mapping; Escherichia coli; Genes, Bacterial; Genes, Recessive; Genetic Markers; Mutation; Phenotype; Plasmids; Recombination, Genetic; Thymine

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.181708.Z4f988805572d2&page=181708&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
Note: ''recQ1'' also blocks the RecF pathway due to deficiency in conjugational recombination when placed in the ''recBC sbcB'' background.

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="W4f9888056268e" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.181708.W4f9888056268e&page=181708&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:6381965

2012-04-25T23:29:20Z

Chrischen: /* Significance {{HelpIcon|Help:Literature}} */

{{RightTOC}}


{| id="Z4f988805572d2" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Nakayama, H, Nakayama, K, Nakayama, R, Irino, N, Nakayama, Y and Hanawalt, PC''' (1984) Isolation and genetic characterization of a thymineless death-resistant mutant of Escherichia coli K12: identification of a new mutation (recQ1) that blocks the RecF recombination pathway.''Mol. Gen. Genet.'' '''195''':474-80
|-
!align=left |Abstract
||
An Escherichia coli K12 mutant resistant to thymineless death (TLD) was isolated, and its genetic analysis led us to identify a new mutation (recQ1) located between corA and metE on the standard linkage map. The mutation was found to result in increased sensitivity to ultraviolet light and deficiency in conjugational recombination when placed in the recBC sbcB background, indicating that it blocked the RecF pathway of recombination. It seemed likely that this mutation is also capable of causing partial resistance to TLD, but we reserve the possibility of a separate mutation closely linked to recQ1 giving rise to this phenotype. The original mutant was shown to carry an additional mutation probably in the vicinity of the uhp locus, which was also required for the full TLD resistance of the mutant to be expressed.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6381965 PubMed]

|-
!align=left |Keywords
||
Chromosome Mapping; Escherichia coli; Genes, Bacterial; Genes, Recessive; Genetic Markers; Mutation; Phenotype; Plasmids; Recombination, Genetic; Thymine

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.181708.Z4f988805572d2&page=181708&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
Note: Mutation also blocks the RecF pathway due to deficiency in conjugational recombination when placed in the ''recBC sbcB'' background.

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="W4f9888056268e" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.181708.W4f9888056268e&page=181708&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:6381965

2012-04-25T23:25:57Z

Chrischen: Fill PMID: Page!

{{RightTOC}}


{| id="Z4f988805572d2" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Nakayama, H, Nakayama, K, Nakayama, R, Irino, N, Nakayama, Y and Hanawalt, PC''' (1984) Isolation and genetic characterization of a thymineless death-resistant mutant of Escherichia coli K12: identification of a new mutation (recQ1) that blocks the RecF recombination pathway.''Mol. Gen. Genet.'' '''195''':474-80
|-
!align=left |Abstract
||
An Escherichia coli K12 mutant resistant to thymineless death (TLD) was isolated, and its genetic analysis led us to identify a new mutation (recQ1) located between corA and metE on the standard linkage map. The mutation was found to result in increased sensitivity to ultraviolet light and deficiency in conjugational recombination when placed in the recBC sbcB background, indicating that it blocked the RecF pathway of recombination. It seemed likely that this mutation is also capable of causing partial resistance to TLD, but we reserve the possibility of a separate mutation closely linked to recQ1 giving rise to this phenotype. The original mutant was shown to carry an additional mutation probably in the vicinity of the uhp locus, which was also required for the full TLD resistance of the mutant to be expressed.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6381965 PubMed]

|-
!align=left |Keywords
||
Chromosome Mapping; Escherichia coli; Genes, Bacterial; Genes, Recessive; Genetic Markers; Mutation; Phenotype; Plasmids; Recombination, Genetic; Thymine

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.181708.Z4f988805572d2&page=181708&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="W4f9888056268e" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.181708.W4f9888056268e&page=181708&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:6381965

2012-04-25T23:25:57Z

Chrischen: New PMID: Page!

recQ:Gene

2012-04-25T23:25:43Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|recQ}}{{Tab On|Gene|recQ}}{{Tab|Gene Product(s)|recQ}}{{Tab|Expression|recQ}}{{Tab|Evolution|recQ}}{{Tab|On One Page|recQ}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="O46a9ffd677cf5" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''recQ''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Recombination
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK3816, b3822, JW5855''<ref name='LIB:Riley_2006'/>, JW5855''

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18207.O46a9ffd677cf5&page=18207&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="B46a9ffd705fc0" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
86.3 minutes 
|
MG1655: 4003887..4005716 <gbrowseImage>
name=NC_000913:4003887..4005716
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10833 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4002887|4006716|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4003887|4005716|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10833 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 3970285..3972120 <gbrowseImage>
name=NC_012967:3970285..3972120
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|3970285|3972120|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|3970285|3972120|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 3893556..3895385 <gbrowseImage>
name=NC_012759:3893556..3895385
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|3893556|3895385|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|3893556|3895385|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 3630817..3628988 <gbrowseImage>
name=AC_000091:3628988..3630817
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|3627988|3631817|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|3628988|3630817|FASTA Dump}}
|-
|
[[DH10B]]
|

|
DH10B: 4102807..4104636 <gbrowseImage>
name=NC_010473:4102807..4104636
source=DH10B
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|DH10B|NC_010473|4099149|4108294|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|4099149|4108294|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18207.B46a9ffd705fc0&page=18207&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="H46a9ffd7bf015" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4003890
|
Edman degradation
|
PMID:2164680 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18207.H46a9ffd7bf015&page=18207&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="Y46a9ffd815243" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
ΔrecQ (Keio:JW5855)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
|

|-
|
''recQ1803::Tn3''
|

|

|

|

|
PMID:2993821
|
[[cgscmutation:65458|CGSC:65458]]
|

|-
|
''recQ1'' (HN15)
|

|

|
Resistant to
|
Mutation causes partial resistance to thymineless death (TLD), figure 1.
|
PMID:6381965
|

|
Parent Strain: AB2497

Mutant previously designated ''tld-1''
|-
|
''recQ1'' (HN15
|

|

|
Sensitivity to
|
Mutation causes increased UV sensitivity in a ''recBC sbcB'' background. (Data not shown)
|
PMID:6381965
|

|
Parent Strain: AB2497

Mutant previously designated ''tld-1''

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18207.Y46a9ffd815243&page=18207&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="G4e04c230ba56c" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18207.G4e04c230ba56c&page=18207&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="X46a9ffd8cdf6e" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW5855
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGCGCAGGCGGAAGTGTTGAA

Primer 2:CCtTCTTCGTCATCGCCATCAAC
|-
|
[[:Category:Kohara_Phage:1C10|1C10]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=551 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:7G3|7G3]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=552 Genobase]
|
PMID:3038334
|-
|
[[ilvD:Gene|ilvD500::Tn10]]
|
Linked marker
|
CAG18431 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40338 CGSC7462]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 9% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:ilv500::Tn10
|-
|
metEo-3079::Tn10
|
Linked marker
|
CAG18491 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40350 CGSC7464]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 85% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18207.X46a9ffd8cdf6e&page=18207&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="R46a9ffd979bdf" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10833 ECOCYC:EG10833]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG10833 EcoGene:EG10833]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948318 GeneID:948318]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18207.R46a9ffd979bdf&page=18207&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="D46a719bcc09a9" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18207.D46a719bcc09a9&page=18207&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:rec genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

recO:Gene

2012-04-24T22:52:01Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|recO}}{{Tab On|Gene|recO}}{{Tab|Gene Product(s)|recO}}{{Tab|Expression|recO}}{{Tab|Evolution|recO}}{{Tab|On One Page|recO}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="U46a9e10d9d604" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''recO''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Recombination
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK2563, b2565, JW2549''<ref name='LIB:Riley_2006'/>, JW2549''

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15701.U46a9e10d9d604&page=15701&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="H46a9e10e273a0" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
58.19 minutes 
|
MG1655: 2700491..2699763 <gbrowseImage>
name=NC_000913:2699763..2700491
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10832 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|2698763|2701491|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|2699763|2700491|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10832 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 2623961..2623233 <gbrowseImage>
name=NC_012967:2623233..2623961
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|2623233|2623961|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|2623233|2623961|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 2585568..2586296 <gbrowseImage>
name=NC_012759:2585568..2586296
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|2585568|2586296|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|2585568|2586296|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 2701125..2700397 <gbrowseImage>
name=AC_000091:2700397..2701125
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|2699397|2702125|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|2700397|2701125|FASTA Dump}}
|-
|
[[DH10B]]
|

|
DH10B: 2792256..2791528 <gbrowseImage>
name=NC_010473:2791528..2792256
source=DH10B
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|DH10B|NC_010473|2790072|2793712|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|2790072|2793712|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15701.H46a9e10e273a0&page=15701&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="H46a9e10ed4a88" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15701.H46a9e10ed4a88&page=15701&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="C46a9e10f2720c" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
ΔrecO (Keio:JW2549)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] [http://cgsc2.biology.yale.edu/Strain.php?ID=108309 CGSC10028]<ref name='LIB:cgsc'/>
|

|-
|
''ΔrecO737::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:102630|CGSC:102630]]
|

|-
|
recO
|
deletion
|

|
Sensitivity to
|
increases sensitivity to [[Bicyclomycin]]
|
PMID:21357484
|

|
fig 2
|-
|
''recO1504''::Tn''5'' (KD2240)
|

|

|
Resistant to
|
Mutation increases resistance to Thymineless Death (TLD). Figure 1.
|
PMID:2848620
|

|
Parent Strain: KD2197

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15701.C46a9e10f2720c&page=15701&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="W4e04c22fa7a4c" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.15701.W4e04c22fa7a4c&page=15701&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="T46a9e10fd4f2d" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|

|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:

Primer 1:

Primer 2:
|-
|
[[:Category:Kohara_Phage:7G4|7G4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=434 Genobase]
|
PMID:3038334
|-
|
[[hcaT:Gene|zfh-208::Tn10]]
|
Linked marker
|
CAG18481 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40086 CGSC7417]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zff-208::Tn10
|-
|
[[nadB:Gene|nadB51::Tn10]]
|
Linked marker
|
CAG18480 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40090 CGSC7419]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 72% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15701.T46a9e10fd4f2d&page=15701&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="C46a9e11077356" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10832 ECOCYC:EG10832]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG10832 EcoGene:EG10832]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=947038 GeneID:947038]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15701.C46a9e11077356&page=15701&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="O46a6fdc99a666" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15701.O46a6fdc99a666&page=15701&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:rec genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

recJ:Gene

2012-04-24T22:51:13Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|recJ}}{{Tab On|Gene|recJ}}{{Tab|Gene Product(s)|recJ}}{{Tab|Expression|recJ}}{{Tab|Evolution|recJ}}{{Tab|On One Page|recJ}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="T46a9e8b4c56b5" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''recJ''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Recombination
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK2887, b2892, JW2860''<ref name='LIB:Riley_2006'/>, JW2860''

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.T46a9e8b4c56b5&page=16349&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="Q46a9e8b550bcc" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
65.4 minutes 
|
MG1655: 3036128..3034395 <gbrowseImage>
name=NC_000913:3034395..3036128
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10830 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|3033395|3037128|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|3034395|3036128|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10830 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 2923859..2922126 <gbrowseImage>
name=NC_012967:2922126..2923859
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|2922126|2923859|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|2922126|2923859|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 2921543..2923276 <gbrowseImage>
name=NC_012759:2921543..2923276
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|2921543|2923276|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|2921543|2923276|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 3036762..3035029 <gbrowseImage>
name=AC_000091:3035029..3036762
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|3034029|3037762|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|3035029|3036762|FASTA Dump}}
|-
|
[[DH10B]]
|

|
DH10B: 3129998..3128265 <gbrowseImage>
name=NC_010473:3128265..3129998
source=DH10B
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|DH10B|NC_010473|3124799|3133464|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|3124799|3133464|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.Q46a9e8b550bcc&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="O46a9e8b60cf78" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.O46a9e8b60cf78&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="U46a9e8b65428d" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
ΔrecJ (Keio:JW2860)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] [http://cgsc2.biology.yale.edu/Strain.php?ID=108498 CGSC10217]<ref name='LIB:cgsc'/>
|

|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009492 recJ::Tn5KAN-I-SceI] (FB20963)
|
Insertion at nt 1026 in Minus orientation
|

|

|

|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20963]
|
contains [[pKD46]]
|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009492 recJ::Tn5KAN-I-SceI] (FB20964)
|
Insertion at nt 1026 in Minus orientation
|

|

|

|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20964]
|
does not contain [[pKD46]]
|-
|
recJ284::Tn10
|
Insertion of Tn10 (Tetracycline-resistance) at nucleotide 434 relative to GTG start codon
|
truncation
|

|
null phenotype, recombination deficient and UV-sensitive in recBC sbcA and recBC sbcBC backgrounds. Highly synergistic for UV-sensitivity, recombination deficiency and partial inviability with recBC.
|
<ref name= 'PMID:6317649'/>
|
CGSC
|

|-
|
recJ77
|
G to A nucleotide 281
|
G to D amino acid 78
|

|
similar to recJ284, null
|
<ref name= 'PMID:6317649'/>
<ref name= 'PMID:6257642'/>
|

|

|-
|
recJ153
recJ154
|
GTG to GTA at start codon
|
None
|

|
Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation
|
<ref name= 'PMID:4587405'/>
<ref name = 'PMID:6317649'/>
<ref name= 'PMID:9352920'/>
|

|

|-
|

|

|

|

|

|

|

|

|-
|
''recJ153''
|

|

|

|

|

|
[[cgscmutation:7801|CGSC:7801]]
|

|-
|
''recJ284''::Tn''10'' (KD2238)
|

|

|
Resistant to
|
Mutation increases resistance to Thymineless Death (TLD). Figure 1.
|
PMID:2848620
|
[[cgscmutation:72003|CGSC:72003]]
|
Parent Strain: KD2197
|-
|
''ΔrecJ743::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:102628|CGSC:102628]]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.U46a9e8b65428d&page=16349&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="N4e04c22cf39a1" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.16349.N4e04c22cf39a1&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="T46a9e8b710fcc" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW2860
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCAAACAACAGATACAACTTCG

Primer 2:CCAATTGGCCAGATATTGTCGAT
|-
|
[[:Category:Kohara_Phage:5B4|5B4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=467 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:22H4|22H4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=468 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:5E11|5E11]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=469 Genobase]
|
PMID:3038334
|-
|
[[recD:Gene|recD1901::Tn10]]
|
Linked marker
|
CAG12135 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40106 CGSC7429]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[speA:Gene|speA210::Tn10]]
|
Linked marker
|
CAG12168 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40110 CGSC7431]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 9% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zgd-210::Tn10, zgf-210::Tn10

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.T46a9e8b710fcc&page=16349&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="B46a9e8b7aa66f" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10830 ECOCYC:EG10830]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG10830 EcoGene:EG10830]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=947367 GeneID:947367]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.B46a9e8b7aa66f&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="Y46a704d3eefc0" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.Y46a704d3eefc0&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:rec genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

PMID:2848620

2012-04-24T22:45:55Z

Chrischen: /* Significance {{HelpIcon|Help:Literature}} */

{{RightTOC}}


{| id="K4f972c91b0cb9" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Nakayama, K, Shiota, S and Nakayama, H''' (1988) Thymineless death in Escherichia coli mutants deficient in the RecF recombination pathway.''Can. J. Microbiol.'' '''34''':905-7
|-
!align=left |Abstract
||
Like recF and recQ mutants studied earlier, two other classes of Escherichia coli mutants defective in the RecF conjugal recombination pathway, recJ and recO, were found to be partially resistant to thymineless death. In contrast, a recN mutant, also belonging to the pathway, was indistinguishable from the wild type with respect to thymineless death.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2848620 PubMed]

|-
!align=left |Keywords
||
Bacterial Proteins; Escherichia coli; Genes, Bacterial; Mutation; Recombination, Genetic; Thymine; Transduction, Genetic

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.181701.K4f972c91b0cb9&page=181701&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}
Note: The resistance is caused by the mutant's defective RecF conjugal recombination pathway. However, this does not confer complete TLD resistance.

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="B4f972c91bb63c" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.181701.B4f972c91bb63c&page=181701&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:2848620

2012-04-24T22:43:29Z

Chrischen: Fill PMID: Page!

{{RightTOC}}


{| id="K4f972c91b0cb9" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Nakayama, K, Shiota, S and Nakayama, H''' (1988) Thymineless death in Escherichia coli mutants deficient in the RecF recombination pathway.''Can. J. Microbiol.'' '''34''':905-7
|-
!align=left |Abstract
||
Like recF and recQ mutants studied earlier, two other classes of Escherichia coli mutants defective in the RecF conjugal recombination pathway, recJ and recO, were found to be partially resistant to thymineless death. In contrast, a recN mutant, also belonging to the pathway, was indistinguishable from the wild type with respect to thymineless death.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2848620 PubMed]

|-
!align=left |Keywords
||
Bacterial Proteins; Escherichia coli; Genes, Bacterial; Mutation; Recombination, Genetic; Thymine; Transduction, Genetic

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.181701.K4f972c91b0cb9&page=181701&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="B4f972c91bb63c" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.181701.B4f972c91bb63c&page=181701&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:2848620

2012-04-24T22:43:29Z

Chrischen: New PMID: Page!

recJ:Gene

2012-04-24T22:42:52Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|recJ}}{{Tab On|Gene|recJ}}{{Tab|Gene Product(s)|recJ}}{{Tab|Expression|recJ}}{{Tab|Evolution|recJ}}{{Tab|On One Page|recJ}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="T46a9e8b4c56b5" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''recJ''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Recombination
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK2887, b2892, JW2860''<ref name='LIB:Riley_2006'/>, JW2860''

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.T46a9e8b4c56b5&page=16349&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="Q46a9e8b550bcc" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
65.4 minutes 
|
MG1655: 3036128..3034395 <gbrowseImage>
name=NC_000913:3034395..3036128
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10830 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|3033395|3037128|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|3034395|3036128|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10830 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 2923859..2922126 <gbrowseImage>
name=NC_012967:2922126..2923859
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|2922126|2923859|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|2922126|2923859|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 2921543..2923276 <gbrowseImage>
name=NC_012759:2921543..2923276
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|2921543|2923276|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|2921543|2923276|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 3036762..3035029 <gbrowseImage>
name=AC_000091:3035029..3036762
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|3034029|3037762|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|3035029|3036762|FASTA Dump}}
|-
|
[[DH10B]]
|

|
DH10B: 3129998..3128265 <gbrowseImage>
name=NC_010473:3128265..3129998
source=DH10B
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|DH10B|NC_010473|3124799|3133464|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|3124799|3133464|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.Q46a9e8b550bcc&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="O46a9e8b60cf78" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.O46a9e8b60cf78&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="U46a9e8b65428d" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
ΔrecJ (Keio:JW2860)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] [http://cgsc2.biology.yale.edu/Strain.php?ID=108498 CGSC10217]<ref name='LIB:cgsc'/>
|

|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009492 recJ::Tn5KAN-I-SceI] (FB20963)
|
Insertion at nt 1026 in Minus orientation
|

|

|

|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20963]
|
contains [[pKD46]]
|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009492 recJ::Tn5KAN-I-SceI] (FB20964)
|
Insertion at nt 1026 in Minus orientation
|

|

|

|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20964]
|
does not contain [[pKD46]]
|-
|
recJ284::Tn10
|
Insertion of Tn10 (Tetracycline-resistance) at nucleotide 434 relative to GTG start codon
|
truncation
|

|
null phenotype, recombination deficient and UV-sensitive in recBC sbcA and recBC sbcBC backgrounds. Highly synergistic for UV-sensitivity, recombination deficiency and partial inviability with recBC.
|
<ref name= 'PMID:6317649'/>
|
CGSC
|

|-
|
recJ77
|
G to A nucleotide 281
|
G to D amino acid 78
|

|
similar to recJ284, null
|
<ref name= 'PMID:6317649'/>
<ref name= 'PMID:6257642'/>
|

|

|-
|
recJ153
recJ154
|
GTG to GTA at start codon
|
None
|

|
Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation
|
<ref name= 'PMID:4587405'/>
<ref name = 'PMID:6317649'/>
<ref name= 'PMID:9352920'/>
|

|

|-
|

|

|

|

|

|

|

|

|-
|
''recJ153''
|

|

|

|

|

|
[[cgscmutation:7801|CGSC:7801]]
|

|-
|
''recJ284::Tn10 (KD2238)''
|

|

|
Resistant to
|
Mutation increases resistance to Thymineless Death (TLD). Figure 1.
|
PMID:2848620
|
[[cgscmutation:72003|CGSC:72003]]
|
Parent Strain: KD2197
|-
|
''ΔrecJ743::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:102628|CGSC:102628]]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.U46a9e8b65428d&page=16349&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="N4e04c22cf39a1" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.16349.N4e04c22cf39a1&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="T46a9e8b710fcc" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW2860
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCAAACAACAGATACAACTTCG

Primer 2:CCAATTGGCCAGATATTGTCGAT
|-
|
[[:Category:Kohara_Phage:5B4|5B4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=467 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:22H4|22H4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=468 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:5E11|5E11]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=469 Genobase]
|
PMID:3038334
|-
|
[[recD:Gene|recD1901::Tn10]]
|
Linked marker
|
CAG12135 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40106 CGSC7429]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[speA:Gene|speA210::Tn10]]
|
Linked marker
|
CAG12168 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40110 CGSC7431]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 9% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zgd-210::Tn10, zgf-210::Tn10

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.T46a9e8b710fcc&page=16349&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="B46a9e8b7aa66f" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10830 ECOCYC:EG10830]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG10830 EcoGene:EG10830]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=947367 GeneID:947367]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.B46a9e8b7aa66f&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="Y46a704d3eefc0" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.Y46a704d3eefc0&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:rec genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

recJ:Gene

2012-04-24T22:41:14Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|recJ}}{{Tab On|Gene|recJ}}{{Tab|Gene Product(s)|recJ}}{{Tab|Expression|recJ}}{{Tab|Evolution|recJ}}{{Tab|On One Page|recJ}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="T46a9e8b4c56b5" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''recJ''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Recombination
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK2887, b2892, JW2860''<ref name='LIB:Riley_2006'/>, JW2860''

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.T46a9e8b4c56b5&page=16349&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="Q46a9e8b550bcc" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
65.4 minutes 
|
MG1655: 3036128..3034395 <gbrowseImage>
name=NC_000913:3034395..3036128
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10830 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|3033395|3037128|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|3034395|3036128|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10830 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 2923859..2922126 <gbrowseImage>
name=NC_012967:2922126..2923859
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|2922126|2923859|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|2922126|2923859|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 2921543..2923276 <gbrowseImage>
name=NC_012759:2921543..2923276
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|2921543|2923276|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|2921543|2923276|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 3036762..3035029 <gbrowseImage>
name=AC_000091:3035029..3036762
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|3034029|3037762|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|3035029|3036762|FASTA Dump}}
|-
|
[[DH10B]]
|

|
DH10B: 3129998..3128265 <gbrowseImage>
name=NC_010473:3128265..3129998
source=DH10B
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|DH10B|NC_010473|3124799|3133464|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|3124799|3133464|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.Q46a9e8b550bcc&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="O46a9e8b60cf78" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.O46a9e8b60cf78&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="U46a9e8b65428d" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
ΔrecJ (Keio:JW2860)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] [http://cgsc2.biology.yale.edu/Strain.php?ID=108498 CGSC10217]<ref name='LIB:cgsc'/>
|

|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009492 recJ::Tn5KAN-I-SceI] (FB20963)
|
Insertion at nt 1026 in Minus orientation
|

|

|

|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20963]
|
contains [[pKD46]]
|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009492 recJ::Tn5KAN-I-SceI] (FB20964)
|
Insertion at nt 1026 in Minus orientation
|

|

|

|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20964]
|
does not contain [[pKD46]]
|-
|
recJ284::Tn10
|
Insertion of Tn10 (Tetracycline-resistance) at nucleotide 434 relative to GTG start codon
|
truncation
|

|
null phenotype, recombination deficient and UV-sensitive in recBC sbcA and recBC sbcBC backgrounds. Highly synergistic for UV-sensitivity, recombination deficiency and partial inviability with recBC.
|
<ref name= 'PMID:6317649'/>
|
CGSC
|

|-
|
recJ77
|
G to A nucleotide 281
|
G to D amino acid 78
|

|
similar to recJ284, null
|
<ref name= 'PMID:6317649'/>
<ref name= 'PMID:6257642'/>
|

|

|-
|
recJ153
recJ154
|
GTG to GTA at start codon
|
None
|

|
Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation
|
<ref name= 'PMID:4587405'/>
<ref name = 'PMID:6317649'/>
<ref name= 'PMID:9352920'/>
|

|

|-
|

|

|

|

|

|

|

|

|-
|
''recJ153''
|

|

|

|

|

|
[[cgscmutation:7801|CGSC:7801]]
|

|-
|
''KD2238 recJ284::Tn10''
|

|

|
Resistant to
|
Mutation increases resistance to Thymineless Death (TLD). Figure 1.
|
PMID:2848620
|
[[cgscmutation:72003|CGSC:72003]]
|
Parent Strain: KD2197
|-
|
''ΔrecJ743::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:102628|CGSC:102628]]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.U46a9e8b65428d&page=16349&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="N4e04c22cf39a1" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.16349.N4e04c22cf39a1&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="T46a9e8b710fcc" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW2860
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCAAACAACAGATACAACTTCG

Primer 2:CCAATTGGCCAGATATTGTCGAT
|-
|
[[:Category:Kohara_Phage:5B4|5B4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=467 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:22H4|22H4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=468 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:5E11|5E11]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=469 Genobase]
|
PMID:3038334
|-
|
[[recD:Gene|recD1901::Tn10]]
|
Linked marker
|
CAG12135 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40106 CGSC7429]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[speA:Gene|speA210::Tn10]]
|
Linked marker
|
CAG12168 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40110 CGSC7431]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 9% <ref name='LIB:Nichols_linked_Tn'/> 
Synonyms:zgd-210::Tn10, zgf-210::Tn10

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.T46a9e8b710fcc&page=16349&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="B46a9e8b7aa66f" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10830 ECOCYC:EG10830]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG10830 EcoGene:EG10830]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=947367 GeneID:947367]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.B46a9e8b7aa66f&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="Y46a704d3eefc0" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16349.Y46a704d3eefc0&page=16349&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:rec genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

uvrA:Gene

2012-03-21T23:50:08Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|uvrA}}{{Tab On|Gene|uvrA}}{{Tab|Gene Product(s)|uvrA}}{{Tab|Expression|uvrA}}{{Tab|Evolution|uvrA}}{{Tab|On One Page|uvrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="A46aa05ee4618e" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''uvrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Ultraviolet resistant
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK4050, b4058, JW4019, dar, dinE''<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.A46aa05ee4618e&page=18675&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="I46aa05eec9b28" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
92.01 minutes, 92.01 minutes 
|
MG1655: 4271894..4269072 <gbrowseImage>
name=NC_000913:4269072..4271894
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11061 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4268072|4272894|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4269072|4271894|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11061 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 4252803..4249981 <gbrowseImage>
name=NC_012967:4249981..4252803
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|4249981|4252803|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4249981|4252803|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 4207917..4210739 <gbrowseImage>
name=NC_012759:4207917..4210739
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|4207917|4210739|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4207917|4210739|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4277461..4274639 <gbrowseImage>
name=AC_000091:4274639..4277461
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|4273639|4278461|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|4274639|4277461|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.I46aa05eec9b28&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="F46aa05ef909f4" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4269072
|
Edman degradation
|
PMID:3007478 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.F46aa05ef909f4&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="O46aa05efdbaa3" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
Strain DH10B
|

|
V332L
|

|
Nonsynonomous mutation
|
PMID:18245285
|
[https://asap.ahabs.wisc.edu/asap/home.php ASAP]
|
This is a SNP in E. coli K-12 Strain [[DH10B]]
|-
|
''uvrA(del)'' (Keio:JW4019)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]

[http://cgsc2.biology.yale.edu/Strain.php?ID=109170 CGSC10889]<ref name='LIB:cgsc'/>
|

|-
|
uvrAC253A,H,S
|

|
C253A,H,S
|

|
Reduced activity
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0A698 UniProt:P0A698]
|-
|
''uvrA6''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:4736|CGSC:4736]]
|
Figure 5

Strain AB1157
|-
|
''uvrA157''
|

|

|

|

|

|
[[cgscmutation:4765|CGSC:4765]]
|

|-
|
''uvrA103''
|

|

|

|

|

|
[[cgscmutation:7745|CGSC:7745]]
|

|-
|
''uvrA155''
|

|

|

|

|

|
[[cgscmutation:8494|CGSC:8494]]
|

|-
|
''uvrA277::Tn10''
|

|

|

|

|

|
[[cgscmutation:8866|CGSC:8866]]
|

|-
|
''uvrA37''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:9254|CGSC:9254]]
|
Figure 5

Strain Hfr J2
|-
|
''uvrA14''
|

|

|

|

|

|
[[cgscmutation:9283|CGSC:9283]]
|

|-
|
''uvrA18''
|

|

|

|

|

|
[[cgscmutation:9291|CGSC:9291]]
|

|-
|
''uvrA19''
|

|

|

|

|

|
[[cgscmutation:9293|CGSC:9293]]
|

|-
|
''uvrA281''
|

|

|

|

|

|
[[cgscmutation:10632|CGSC:10632]]
|

|-
|
''uvrA154''
|

|

|

|

|

|
[[cgscmutation:11960|CGSC:11960]]
|

|-
|
''uvrA0''
|

|

|

|

|

|
[[cgscmutation:17383|CGSC:17383]]
|

|-
|
''uvrA215::Mud(Ap,lac)''
|

|

|

|

|
PMID:6771759 PMID:6780917
|
[[cgscmutation:67398|CGSC:67398]]
|

|-
|
''uvrA753(del)::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:103342|CGSC:103342]]
|

|-
|
''uvrA(del)::Tn10''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.
|
PMID:17040104
|
Experimental strain:GY5564
|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.
|
PMID:17040104
|

|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.
|
PMID:16970815
|

|
figure 2
|-
|
''uvrA'' K689A
|

|
Lysine to Alanine
|
change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680E
|

|
Lysine to Glutamic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691A
|

|
Arginine to Alanine
|
Change in DNA binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691D
|

|
Arginine to Aspartic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680A R691A
|

|
Lysine to Alanine & Arginine to Alanine
|
Change in binding affinity
|
Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
DNA repair
|
Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.
|
PMID:17040104
|

|
Figure 4
|-
|
''uvrAB'' K45A
|

|
Lysine to alanine
|
change in enzyme activity
|
ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.
|
PMID:17630776
|

|

|-
|
''ZnG uvrA''
|

|
11 amino acid change, figure 1.
|
DNA binding
|
Mutant shows decreased binding affinity to damaged DNA, table 1.
|
PMID:16829526
|

|

|-
|
''uvrA'' G502D V508D
|

|
Glycine to Aspartic Acid & Valine to Aspartic Acid
|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation by causing the loss of uvrA's binding specificity between damaged and undamaged DNA. Figure 7.
|
PMID:8444906
|

|
Figure 7

Parent Strain: MH1-∆A
|-
|
''uvrA'' G275A
|

|
Glycine to Alanine
|
Resistant to
|
Mutation creates "hyper" UV resistance greater than the UV resistance of wild type, figure 4.
|
PMID:8444906
|

|
Figure 4
|-
|
''uvrA'' C763F
|

|
Cysteine to Phenylalanine
|
Change in DNA binding
|
Mutation causes loss of single-stranded DNA-binding activity.
|
PMID:8444906
|

|
No data shown

Parent Strain: MH1-∆A
|-
|
''uvrA'' C740F H754Y C742Y/K750I
|

|
Cysteine to Phenylalanine & Histidine to Tyrosine & Cysteine to Tyrosine/Lysine to Isoleucine (double mutant)
|
Sensitivity to
|
Mutation increased UV sensitivity by making the mutant proteins excision repair defective
|
PMID:8444906
|

|
No data shown in source.

Parent Strain: MH1-∆A

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.O46aa05efdbaa3&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="S4e04c56173535" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18675.S4e04c56173535&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="L46aa05f0a24e1" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW4019
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG
|-
|
[[:Category:Kohara_Phage:12D1|12D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=637 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3F12|3F12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=638 Genobase]
|
PMID:3038334
|-
|
[[malF:Gene|malF3089::Tn10]]
|
Linked marker
|
CAG12164 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40371 CGSC7476]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[yjcS:Gene|zje-2241::Tn10]]
|
Linked marker
|
CAG18427 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40603 CGSC7481]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46aa05f0a24e1&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="X46aa05f14ff9b" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948559 GeneID:948559]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.X46aa05f14ff9b&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="L46a71f2747b21" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46a71f2747b21&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:uvr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

uvrA:Gene

2012-03-21T23:37:21Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|uvrA}}{{Tab On|Gene|uvrA}}{{Tab|Gene Product(s)|uvrA}}{{Tab|Expression|uvrA}}{{Tab|Evolution|uvrA}}{{Tab|On One Page|uvrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="A46aa05ee4618e" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''uvrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Ultraviolet resistant
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK4050, b4058, JW4019, dar, dinE''<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.A46aa05ee4618e&page=18675&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="I46aa05eec9b28" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
92.01 minutes, 92.01 minutes 
|
MG1655: 4271894..4269072 <gbrowseImage>
name=NC_000913:4269072..4271894
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11061 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4268072|4272894|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4269072|4271894|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11061 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 4252803..4249981 <gbrowseImage>
name=NC_012967:4249981..4252803
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|4249981|4252803|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4249981|4252803|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 4207917..4210739 <gbrowseImage>
name=NC_012759:4207917..4210739
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|4207917|4210739|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4207917|4210739|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4277461..4274639 <gbrowseImage>
name=AC_000091:4274639..4277461
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|4273639|4278461|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|4274639|4277461|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.I46aa05eec9b28&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="F46aa05ef909f4" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4269072
|
Edman degradation
|
PMID:3007478 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.F46aa05ef909f4&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="O46aa05efdbaa3" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
Strain DH10B
|

|
V332L
|

|
Nonsynonomous mutation
|
PMID:18245285
|
[https://asap.ahabs.wisc.edu/asap/home.php ASAP]
|
This is a SNP in E. coli K-12 Strain [[DH10B]]
|-
|
''uvrA(del)'' (Keio:JW4019)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]

[http://cgsc2.biology.yale.edu/Strain.php?ID=109170 CGSC10889]<ref name='LIB:cgsc'/>
|

|-
|
uvrAC253A,H,S
|

|
C253A,H,S
|

|
Reduced activity
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0A698 UniProt:P0A698]
|-
|
''uvrA6''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:4736|CGSC:4736]]
|
Figure 5

Strain AB1157
|-
|
''uvrA157''
|

|

|

|

|

|
[[cgscmutation:4765|CGSC:4765]]
|

|-
|
''uvrA103''
|

|

|

|

|

|
[[cgscmutation:7745|CGSC:7745]]
|

|-
|
''uvrA155''
|

|

|

|

|

|
[[cgscmutation:8494|CGSC:8494]]
|

|-
|
''uvrA277::Tn10''
|

|

|

|

|

|
[[cgscmutation:8866|CGSC:8866]]
|

|-
|
''uvrA37''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:9254|CGSC:9254]]
|
Figure 5

Strain Hfr J2
|-
|
''uvrA14''
|

|

|

|

|

|
[[cgscmutation:9283|CGSC:9283]]
|

|-
|
''uvrA18''
|

|

|

|

|

|
[[cgscmutation:9291|CGSC:9291]]
|

|-
|
''uvrA19''
|

|

|

|

|

|
[[cgscmutation:9293|CGSC:9293]]
|

|-
|
''uvrA281''
|

|

|

|

|

|
[[cgscmutation:10632|CGSC:10632]]
|

|-
|
''uvrA154''
|

|

|

|

|

|
[[cgscmutation:11960|CGSC:11960]]
|

|-
|
''uvrA0''
|

|

|

|

|

|
[[cgscmutation:17383|CGSC:17383]]
|

|-
|
''uvrA215::Mud(Ap,lac)''
|

|

|

|

|
PMID:6771759 PMID:6780917
|
[[cgscmutation:67398|CGSC:67398]]
|

|-
|
''uvrA753(del)::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:103342|CGSC:103342]]
|

|-
|
''uvrA(del)::Tn10''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.
|
PMID:17040104
|
Experimental strain:GY5564
|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.
|
PMID:17040104
|

|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.
|
PMID:16970815
|

|
figure 2
|-
|
''uvrA'' K689A
|

|
Lysine to Alanine
|
change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680E
|

|
Lysine to Glutamic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691A
|

|
Arginine to Alanine
|
Change in DNA binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691D
|

|
Arginine to Aspartic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680A R691A
|

|
Lysine to Alanine & Arginine to Alanine
|
Change in binding affinity
|
Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
DNA repair
|
Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.
|
PMID:17040104
|

|
Figure 4
|-
|
''uvrAB'' K45A
|

|
Lysine to alanine
|
change in enzyme activity
|
ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.
|
PMID:17630776
|

|

|-
|
''ZnG uvrA''
|

|
11 amino acid change, figure 1.
|
DNA binding
|
Mutant shows decreased binding affinity to damaged DNA, table 1.
|
PMID:16829526
|

|

|-
|
''uvrA'' G502D V508D
|

|
Glycine to Aspartic Acid & Valine to Aspartic Acid
|
Sensitivity to
|
Mutation decreases UV-radiation resistance by causing the loss of uvrA's binding specificity between damaged and undamaged DNA. Figure 7.
|
PMID:8444906
|

|
Figure 7

Parent Strain: MH1-∆A
|-
|
''uvrA'' G275A
|

|
Glycine to Alanine
|
Resistant to
|
Mutation creates "hyper" UV-resistant phenotype greater than wild-type's UV-resistance, figure 4.
|
PMID:8444906
|

|
Figure 4
|-
|
''uvrA'' C740F H754Y C742Y/K750I
|

|
Cysteine to Phenylalanine & Histidine to Tyrosine & Cysteine to Tyrosine/Lysine to Isoleucine (double mutant)
|
Sensitivity to
|
Mutation increased UV sensitivity by making the mutant proteins excision repair defective
|
PMID:8444906
|

|
No data shown in source.

Parent Strain: MH1-∆A

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.O46aa05efdbaa3&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="S4e04c56173535" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18675.S4e04c56173535&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="L46aa05f0a24e1" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW4019
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG
|-
|
[[:Category:Kohara_Phage:12D1|12D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=637 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3F12|3F12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=638 Genobase]
|
PMID:3038334
|-
|
[[malF:Gene|malF3089::Tn10]]
|
Linked marker
|
CAG12164 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40371 CGSC7476]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[yjcS:Gene|zje-2241::Tn10]]
|
Linked marker
|
CAG18427 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40603 CGSC7481]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46aa05f0a24e1&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="X46aa05f14ff9b" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948559 GeneID:948559]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.X46aa05f14ff9b&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="L46a71f2747b21" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46a71f2747b21&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:uvr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

uvrA:Gene

2012-03-21T23:30:46Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|uvrA}}{{Tab On|Gene|uvrA}}{{Tab|Gene Product(s)|uvrA}}{{Tab|Expression|uvrA}}{{Tab|Evolution|uvrA}}{{Tab|On One Page|uvrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="A46aa05ee4618e" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''uvrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Ultraviolet resistant
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK4050, b4058, JW4019, dar, dinE''<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.A46aa05ee4618e&page=18675&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="I46aa05eec9b28" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
92.01 minutes, 92.01 minutes 
|
MG1655: 4271894..4269072 <gbrowseImage>
name=NC_000913:4269072..4271894
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11061 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4268072|4272894|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4269072|4271894|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11061 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 4252803..4249981 <gbrowseImage>
name=NC_012967:4249981..4252803
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|4249981|4252803|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4249981|4252803|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 4207917..4210739 <gbrowseImage>
name=NC_012759:4207917..4210739
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|4207917|4210739|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4207917|4210739|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4277461..4274639 <gbrowseImage>
name=AC_000091:4274639..4277461
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|4273639|4278461|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|4274639|4277461|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.I46aa05eec9b28&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="F46aa05ef909f4" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4269072
|
Edman degradation
|
PMID:3007478 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.F46aa05ef909f4&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="O46aa05efdbaa3" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
Strain DH10B
|

|
V332L
|

|
Nonsynonomous mutation
|
PMID:18245285
|
[https://asap.ahabs.wisc.edu/asap/home.php ASAP]
|
This is a SNP in E. coli K-12 Strain [[DH10B]]
|-
|
''uvrA(del)'' (Keio:JW4019)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]

[http://cgsc2.biology.yale.edu/Strain.php?ID=109170 CGSC10889]<ref name='LIB:cgsc'/>
|

|-
|
uvrAC253A,H,S
|

|
C253A,H,S
|

|
Reduced activity
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0A698 UniProt:P0A698]
|-
|
''uvrA6''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:4736|CGSC:4736]]
|
Figure 5

Strain AB1157
|-
|
''uvrA157''
|

|

|

|

|

|
[[cgscmutation:4765|CGSC:4765]]
|

|-
|
''uvrA103''
|

|

|

|

|

|
[[cgscmutation:7745|CGSC:7745]]
|

|-
|
''uvrA155''
|

|

|

|

|

|
[[cgscmutation:8494|CGSC:8494]]
|

|-
|
''uvrA277::Tn10''
|

|

|

|

|

|
[[cgscmutation:8866|CGSC:8866]]
|

|-
|
''uvrA37''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:9254|CGSC:9254]]
|
Figure 5

Strain Hfr J2
|-
|
''uvrA14''
|

|

|

|

|

|
[[cgscmutation:9283|CGSC:9283]]
|

|-
|
''uvrA18''
|

|

|

|

|

|
[[cgscmutation:9291|CGSC:9291]]
|

|-
|
''uvrA19''
|

|

|

|

|

|
[[cgscmutation:9293|CGSC:9293]]
|

|-
|
''uvrA281''
|

|

|

|

|

|
[[cgscmutation:10632|CGSC:10632]]
|

|-
|
''uvrA154''
|

|

|

|

|

|
[[cgscmutation:11960|CGSC:11960]]
|

|-
|
''uvrA0''
|

|

|

|

|

|
[[cgscmutation:17383|CGSC:17383]]
|

|-
|
''uvrA215::Mud(Ap,lac)''
|

|

|

|

|
PMID:6771759 PMID:6780917
|
[[cgscmutation:67398|CGSC:67398]]
|

|-
|
''uvrA753(del)::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:103342|CGSC:103342]]
|

|-
|
''uvrA(del)::Tn10''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.
|
PMID:17040104
|
Experimental strain:GY5564
|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.
|
PMID:17040104
|

|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.
|
PMID:16970815
|

|
figure 2
|-
|
''uvrA'' K689A
|

|
Lysine to Alanine
|
change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680E
|

|
Lysine to Glutamic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691A
|

|
Arginine to Alanine
|
Change in DNA binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691D
|

|
Arginine to Aspartic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680A R691A
|

|
Lysine to Alanine & Arginine to Alanine
|
Change in binding affinity
|
Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
DNA repair
|
Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.
|
PMID:17040104
|

|
Figure 4
|-
|
''uvrAB'' K45A
|

|
Lysine to alanine
|
change in enzyme activity
|
ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.
|
PMID:17630776
|

|

|-
|
''ZnG uvrA''
|

|
11 amino acid change, figure 1.
|
DNA binding
|
Mutant shows decreased binding affinity to damaged DNA, table 1.
|
PMID:16829526
|

|

|-
|
''uvrA'' G502D V508D
|

|
Glycine to Aspartic Acid & Valine to Aspartic Acid
|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation by causing the loss of uvrA's binding specificity between damaged and undamaged DNA. Figure 7.
|
PMID:8444906
|

|
Figure 7

Parent Strain: MH1-∆A
|-
|
''uvrA'' G275A
|

|
Glycine to Alanine
|
Resistant to
|
Mutation creates "hyper" UV resistance greater than the UV resistance of wild type, figure 4.
|
PMID:8444906
|

|
Figure 4
|-
|
''uvrA'' C763F
|

|
Cysteine to Phenylalanine
|
Change in DNA binding
|
Mutation causes loss of single-stranded DNA-binding activity.
|
PMID:8444906
|

|
No data shown

Parent Strain: MH1-∆A

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.O46aa05efdbaa3&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="S4e04c56173535" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18675.S4e04c56173535&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="L46aa05f0a24e1" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW4019
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG
|-
|
[[:Category:Kohara_Phage:12D1|12D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=637 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3F12|3F12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=638 Genobase]
|
PMID:3038334
|-
|
[[malF:Gene|malF3089::Tn10]]
|
Linked marker
|
CAG12164 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40371 CGSC7476]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[yjcS:Gene|zje-2241::Tn10]]
|
Linked marker
|
CAG18427 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40603 CGSC7481]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46aa05f0a24e1&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="X46aa05f14ff9b" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948559 GeneID:948559]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.X46aa05f14ff9b&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="L46a71f2747b21" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46a71f2747b21&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:uvr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

uvrA:Gene

2012-03-21T23:18:49Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|uvrA}}{{Tab On|Gene|uvrA}}{{Tab|Gene Product(s)|uvrA}}{{Tab|Expression|uvrA}}{{Tab|Evolution|uvrA}}{{Tab|On One Page|uvrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="A46aa05ee4618e" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''uvrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Ultraviolet resistant
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK4050, b4058, JW4019, dar, dinE''<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.A46aa05ee4618e&page=18675&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="I46aa05eec9b28" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
92.01 minutes, 92.01 minutes 
|
MG1655: 4271894..4269072 <gbrowseImage>
name=NC_000913:4269072..4271894
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11061 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4268072|4272894|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4269072|4271894|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11061 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 4252803..4249981 <gbrowseImage>
name=NC_012967:4249981..4252803
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|4249981|4252803|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4249981|4252803|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 4207917..4210739 <gbrowseImage>
name=NC_012759:4207917..4210739
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|4207917|4210739|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4207917|4210739|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4277461..4274639 <gbrowseImage>
name=AC_000091:4274639..4277461
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|4273639|4278461|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|4274639|4277461|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.I46aa05eec9b28&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="F46aa05ef909f4" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4269072
|
Edman degradation
|
PMID:3007478 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.F46aa05ef909f4&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="O46aa05efdbaa3" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
Strain DH10B
|

|
V332L
|

|
Nonsynonomous mutation
|
PMID:18245285
|
[https://asap.ahabs.wisc.edu/asap/home.php ASAP]
|
This is a SNP in E. coli K-12 Strain [[DH10B]]
|-
|
''uvrA(del)'' (Keio:JW4019)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]

[http://cgsc2.biology.yale.edu/Strain.php?ID=109170 CGSC10889]<ref name='LIB:cgsc'/>
|

|-
|
uvrAC253A,H,S
|

|
C253A,H,S
|

|
Reduced activity
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0A698 UniProt:P0A698]
|-
|
''uvrA6''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:4736|CGSC:4736]]
|
Figure 5

Strain AB1157
|-
|
''uvrA157''
|

|

|

|

|

|
[[cgscmutation:4765|CGSC:4765]]
|

|-
|
''uvrA103''
|

|

|

|

|

|
[[cgscmutation:7745|CGSC:7745]]
|

|-
|
''uvrA155''
|

|

|

|

|

|
[[cgscmutation:8494|CGSC:8494]]
|

|-
|
''uvrA277::Tn10''
|

|

|

|

|

|
[[cgscmutation:8866|CGSC:8866]]
|

|-
|
''uvrA37''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:9254|CGSC:9254]]
|
Figure 5

Strain Hfr J2
|-
|
''uvrA14''
|

|

|

|

|

|
[[cgscmutation:9283|CGSC:9283]]
|

|-
|
''uvrA18''
|

|

|

|

|

|
[[cgscmutation:9291|CGSC:9291]]
|

|-
|
''uvrA19''
|

|

|

|

|

|
[[cgscmutation:9293|CGSC:9293]]
|

|-
|
''uvrA281''
|

|

|

|

|

|
[[cgscmutation:10632|CGSC:10632]]
|

|-
|
''uvrA154''
|

|

|

|

|

|
[[cgscmutation:11960|CGSC:11960]]
|

|-
|
''uvrA0''
|

|

|

|

|

|
[[cgscmutation:17383|CGSC:17383]]
|

|-
|
''uvrA215::Mud(Ap,lac)''
|

|

|

|

|
PMID:6771759 PMID:6780917
|
[[cgscmutation:67398|CGSC:67398]]
|

|-
|
''uvrA753(del)::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:103342|CGSC:103342]]
|

|-
|
''uvrA(del)::Tn10''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.
|
PMID:17040104
|
Experimental strain:GY5564
|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.
|
PMID:17040104
|

|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.
|
PMID:16970815
|

|
figure 2
|-
|
''uvrA'' K689A
|

|
Lysine to Alanine
|
change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680E
|

|
Lysine to Glutamic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691A
|

|
Arginine to Alanine
|
Change in DNA binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691D
|

|
Arginine to Aspartic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680A R691A
|

|
Lysine to Alanine & Arginine to Alanine
|
Change in binding affinity
|
Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
DNA repair
|
Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.
|
PMID:17040104
|

|
Figure 4
|-
|
''uvrAB'' K45A
|

|
Lysine to alanine
|
change in enzyme activity
|
ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.
|
PMID:17630776
|

|

|-
|
''ZnG uvrA''
|

|
11 amino acid change, figure 1.
|
DNA binding
|
Mutant shows decreased binding affinity to damaged DNA, table 1.
|
PMID:16829526
|

|

|-
|
''uvrA'' G502D V508D
|

|
Glycine to Aspartic Acid & Valine to Aspartic Acid
|
Sensitivity to
|
Mutation decreases UV-radiation resistance by causing the loss of uvrA's binding specificity between damaged and undamaged DNA. Figure 7.
|
PMID:8444906
|

|
Figure 7

Parent Strain: MH1-∆A
|-
|
''uvrA'' G275A
|

|
Glycine to Alanine
|
Resistant to
|
Mutation creates "hyper" UV-resistant phenotype greater than wild-type's UV-resistance, figure 4.
|
PMID:8444906
|

|
Figure 4
|-
|
''uvrA'' C763F
|

|
Cysteine to Phenylalanine
|
Change in DNA binding
|
Mutation causes loss of single-stranded DNA-binding activity.
|
PMID:8444906
|

|
No data shown

Parent Strain: MH1-∆A

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.O46aa05efdbaa3&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="S4e04c56173535" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18675.S4e04c56173535&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="L46aa05f0a24e1" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW4019
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG
|-
|
[[:Category:Kohara_Phage:12D1|12D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=637 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3F12|3F12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=638 Genobase]
|
PMID:3038334
|-
|
[[malF:Gene|malF3089::Tn10]]
|
Linked marker
|
CAG12164 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40371 CGSC7476]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[yjcS:Gene|zje-2241::Tn10]]
|
Linked marker
|
CAG18427 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40603 CGSC7481]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46aa05f0a24e1&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="X46aa05f14ff9b" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948559 GeneID:948559]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.X46aa05f14ff9b&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="L46a71f2747b21" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46a71f2747b21&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:uvr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

uvrA:Gene

2012-03-21T23:09:54Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|uvrA}}{{Tab On|Gene|uvrA}}{{Tab|Gene Product(s)|uvrA}}{{Tab|Expression|uvrA}}{{Tab|Evolution|uvrA}}{{Tab|On One Page|uvrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="A46aa05ee4618e" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''uvrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Ultraviolet resistant
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK4050, b4058, JW4019, dar, dinE''<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.A46aa05ee4618e&page=18675&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="I46aa05eec9b28" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
92.01 minutes, 92.01 minutes 
|
MG1655: 4271894..4269072 <gbrowseImage>
name=NC_000913:4269072..4271894
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11061 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4268072|4272894|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4269072|4271894|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11061 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 4252803..4249981 <gbrowseImage>
name=NC_012967:4249981..4252803
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|4249981|4252803|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4249981|4252803|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 4207917..4210739 <gbrowseImage>
name=NC_012759:4207917..4210739
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|4207917|4210739|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4207917|4210739|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4277461..4274639 <gbrowseImage>
name=AC_000091:4274639..4277461
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|4273639|4278461|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|4274639|4277461|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.I46aa05eec9b28&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="F46aa05ef909f4" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4269072
|
Edman degradation
|
PMID:3007478 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.F46aa05ef909f4&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="O46aa05efdbaa3" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
Strain DH10B
|

|
V332L
|

|
Nonsynonomous mutation
|
PMID:18245285
|
[https://asap.ahabs.wisc.edu/asap/home.php ASAP]
|
This is a SNP in E. coli K-12 Strain [[DH10B]]
|-
|
''uvrA(del)'' (Keio:JW4019)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]

[http://cgsc2.biology.yale.edu/Strain.php?ID=109170 CGSC10889]<ref name='LIB:cgsc'/>
|

|-
|
uvrAC253A,H,S
|

|
C253A,H,S
|

|
Reduced activity
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0A698 UniProt:P0A698]
|-
|
''uvrA6''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:4736|CGSC:4736]]
|
Figure 5

Strain AB1157
|-
|
''uvrA157''
|

|

|

|

|

|
[[cgscmutation:4765|CGSC:4765]]
|

|-
|
''uvrA103''
|

|

|

|

|

|
[[cgscmutation:7745|CGSC:7745]]
|

|-
|
''uvrA155''
|

|

|

|

|

|
[[cgscmutation:8494|CGSC:8494]]
|

|-
|
''uvrA277::Tn10''
|

|

|

|

|

|
[[cgscmutation:8866|CGSC:8866]]
|

|-
|
''uvrA37''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:9254|CGSC:9254]]
|
Figure 5

Strain Hfr J2
|-
|
''uvrA14''
|

|

|

|

|

|
[[cgscmutation:9283|CGSC:9283]]
|

|-
|
''uvrA18''
|

|

|

|

|

|
[[cgscmutation:9291|CGSC:9291]]
|

|-
|
''uvrA19''
|

|

|

|

|

|
[[cgscmutation:9293|CGSC:9293]]
|

|-
|
''uvrA281''
|

|

|

|

|

|
[[cgscmutation:10632|CGSC:10632]]
|

|-
|
''uvrA154''
|

|

|

|

|

|
[[cgscmutation:11960|CGSC:11960]]
|

|-
|
''uvrA0''
|

|

|

|

|

|
[[cgscmutation:17383|CGSC:17383]]
|

|-
|
''uvrA215::Mud(Ap,lac)''
|

|

|

|

|
PMID:6771759 PMID:6780917
|
[[cgscmutation:67398|CGSC:67398]]
|

|-
|
''uvrA753(del)::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:103342|CGSC:103342]]
|

|-
|
''uvrA(del)::Tn10''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.
|
PMID:17040104
|
Experimental strain:GY5564
|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.
|
PMID:17040104
|

|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.
|
PMID:16970815
|

|
figure 2
|-
|
''uvrA'' K689A
|

|
Lysine to Alanine
|
change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680E
|

|
Lysine to Glutamic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691A
|

|
Arginine to Alanine
|
Change in DNA binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691D
|

|
Arginine to Aspartic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680A R691A
|

|
Lysine to Alanine & Arginine to Alanine
|
Change in binding affinity
|
Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
DNA repair
|
Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.
|
PMID:17040104
|

|
Figure 4
|-
|
''uvrAB'' K45A
|

|
Lysine to alanine
|
change in enzyme activity
|
ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.
|
PMID:17630776
|

|

|-
|
''ZnG uvrA''
|

|
11 amino acid change, figure 1.
|
DNA binding
|
Mutant shows decreased binding affinity to damaged DNA, table 1.
|
PMID:16829526
|

|

|-
|
''uvrA'' G502D V508D
|

|
Glycine to Aspartic Acid & Valine to Aspartic Acid
|
Sensitivity to
|
Mutation decreases UV-radiation resistance by causing the loss of uvrA's binding specificity between damaged and undamaged DNA. Figure 7.
|
PMID:8444906
|

|
Figure 7

Parent Strain: MH1-∆A
|-
|
''uvrA'' G275A
|

|
Glycine to Alanine
|
Resistant to
|
Mutation creates "hyper" UV-resistant phenotype greater than wild-type's UV-resistance, figure 4.
|
PMID:8444906
|

|
Figure 4
|-
|
''uvrA'' C763F
|

|
Cysteine to Phenylalanine
|
Protein inactivation
|
Mutation causes loss of DNA-binding activity.
|
PMID:8444906
|

|
No data shown

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.O46aa05efdbaa3&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="S4e04c56173535" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18675.S4e04c56173535&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="L46aa05f0a24e1" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW4019
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG
|-
|
[[:Category:Kohara_Phage:12D1|12D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=637 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3F12|3F12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=638 Genobase]
|
PMID:3038334
|-
|
[[malF:Gene|malF3089::Tn10]]
|
Linked marker
|
CAG12164 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40371 CGSC7476]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[yjcS:Gene|zje-2241::Tn10]]
|
Linked marker
|
CAG18427 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40603 CGSC7481]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46aa05f0a24e1&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="X46aa05f14ff9b" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948559 GeneID:948559]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.X46aa05f14ff9b&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="L46a71f2747b21" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46a71f2747b21&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:uvr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

PMID:8444906

2012-03-20T23:32:29Z

Chrischen: Table edited by Chrischen via TableEdit

{{RightTOC}}


{| id="P4f690da79ba31" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Wang, J and Grossman, L''' (1993) Mutations in the helix-turn-helix motif of the Escherichia coli UvrA protein eliminate its specificity for UV-damaged DNA.''J. Biol. Chem.'' '''268''':5323-31
|-
!align=left |Abstract
||
The Escherichia coli UvrA protein possesses a stretch of amino acids, 494 to 513, that matches the consensus sequence of the helix-turn-helix motif of many sequence-specific DNA binding proteins. It also has two zinc finger motif regions and two ATP binding sites. To study the potential roles of both helix-turn-helix and zinc finger motifs in the functioning of UvrA protein, random mutations were created in these motif regions by degenerate oligonucleotide-directed mutagenesis. Using this method, 12 single substitution mutants (eight in the helix-turn-helix motif region, one in the N-terminal zinc finger region, and three in the C-terminal zinc finger region) were isolated that failed to confer UV resistance in the E. coli strain deleted of the uvrA gene. One "hyper" UV-resistant mutant, G275A, was identified that conferred significantly more UV resistance than the wild type in the MH1-delta A strain. To further investigate the mechanism of failure of these mutant UvrA proteins to support nucleotide excision repair, two mutant UvrA proteins, G502D and V508D, were selected for purification and characterization, since they carry mutations at the positions offered as the putative constellation for the helix-turn-helix motif. The binding affinity of these two mutants for nonirradiated plasmid DNA was unaffected by the mutations. Both mutant proteins exhibited substantial ATPase activity, and together with the UvrB protein, they were capable of generating positively supercoiled plasmid DNA from the relaxed form in the presence of ATP and bacterial topoisomerase I. However, both mutant proteins failed to respond to UV damage in the filter binding assay and were incapable of forming 2 x SSC-resistant nucleoprotein complexes with UvrB protein on UV-irradiated plasmid DNA. Taking these properties together, it appears that the mutations in the helix-turn-helix motif region impaired the UvrA protein's ability to recognize UV damage, while its other activities were largely unaffected. Interestingly, ERCC-3, a human DNA repair protein, also has a similar helix-turn-helix motif. Given the highly conserved nature of repair proteins in general, this observation raises the possibility that both procaryotes and eucaryotes might use similar mechanisms to recognize damaged sites in their genomes.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8444906 PubMed]

|-
!align=left |Keywords
||
Adenosine Triphosphatases; Adenosine Triphosphate; Amino Acid Sequence; Bacterial Proteins; Base Sequence; DNA; DNA Damage; DNA, Superhelical; DNA, Viral; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Hydrolysis; Molecular Sequence Data; Mutation; Protein Conformation; Sequence Homology, Amino Acid; Structure-Activity Relationship; Substrate Specificity; Ultraviolet Rays; Zinc Fingers

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180990.P4f690da79ba31&page=180990&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="W4f690da7b2526" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180990.W4f690da7b2526&page=180990&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>
==Mutant alleles described in the paper==
<protect>

{| id="N4f690e6caac09" class=" tableEdit "
|-
!|Mutant!!Residue Change!!Codon Change!!UV Sensitivity
|-
|
2-13
|
Ser269 to Pro
|
TCG to CCG
|
S
|-
|
3-15
|
Arg494 to Pro
|
CGT to CCT
|
S
|-
|
3-12
|
Ala498 to Glu
|
GCG to CAG
|
S
|-
|
3-5
|
Gly502 to Asp
|
GGT to GAT
|
S
|-
|
4-27
|
Leu505 to Asp
|
CTG to CGG
|
S
|-
|
4-13
|
Val506 to Asp
|
GTT to GAT
|
S
|-
|
4-42
|
Val508 to Asp
|
GTT to GAT
|
S
|-
|
4-7
|
Tyr510 to Phe
|
TAC to TTC
|
S
|-
|
4-4
|
Asp513 to His
|
GAC to CAC
|
S
|-
|
5-12
|
Cys740 to Phe
|
TGC to TTC
|
S
|-
|
5-7
|
Cys742 to Tyr
|
AAA to ATA
|
S
|-
|
5-7
|
Lys750 to Ile
|
TGT to TAT
|

|-
|
6-14
|
His754 to Tyr
|
CAC to TAC
|
S
|-
|
6-12
|
Cys763 to Phe
|
TGC to TTC
|
S
|-
|
6-61
|
Cys763 to Ser
|
TGC to TCC
|
R

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180990.N4f690e6caac09&page=180990&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:8444906

2012-03-20T23:10:36Z

Chrischen: /* Annotations {{HelpIcon|Help:Literature}} */

{{RightTOC}}


{| id="P4f690da79ba31" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Wang, J and Grossman, L''' (1993) Mutations in the helix-turn-helix motif of the Escherichia coli UvrA protein eliminate its specificity for UV-damaged DNA.''J. Biol. Chem.'' '''268''':5323-31
|-
!align=left |Abstract
||
The Escherichia coli UvrA protein possesses a stretch of amino acids, 494 to 513, that matches the consensus sequence of the helix-turn-helix motif of many sequence-specific DNA binding proteins. It also has two zinc finger motif regions and two ATP binding sites. To study the potential roles of both helix-turn-helix and zinc finger motifs in the functioning of UvrA protein, random mutations were created in these motif regions by degenerate oligonucleotide-directed mutagenesis. Using this method, 12 single substitution mutants (eight in the helix-turn-helix motif region, one in the N-terminal zinc finger region, and three in the C-terminal zinc finger region) were isolated that failed to confer UV resistance in the E. coli strain deleted of the uvrA gene. One "hyper" UV-resistant mutant, G275A, was identified that conferred significantly more UV resistance than the wild type in the MH1-delta A strain. To further investigate the mechanism of failure of these mutant UvrA proteins to support nucleotide excision repair, two mutant UvrA proteins, G502D and V508D, were selected for purification and characterization, since they carry mutations at the positions offered as the putative constellation for the helix-turn-helix motif. The binding affinity of these two mutants for nonirradiated plasmid DNA was unaffected by the mutations. Both mutant proteins exhibited substantial ATPase activity, and together with the UvrB protein, they were capable of generating positively supercoiled plasmid DNA from the relaxed form in the presence of ATP and bacterial topoisomerase I. However, both mutant proteins failed to respond to UV damage in the filter binding assay and were incapable of forming 2 x SSC-resistant nucleoprotein complexes with UvrB protein on UV-irradiated plasmid DNA. Taking these properties together, it appears that the mutations in the helix-turn-helix motif region impaired the UvrA protein's ability to recognize UV damage, while its other activities were largely unaffected. Interestingly, ERCC-3, a human DNA repair protein, also has a similar helix-turn-helix motif. Given the highly conserved nature of repair proteins in general, this observation raises the possibility that both procaryotes and eucaryotes might use similar mechanisms to recognize damaged sites in their genomes.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8444906 PubMed]

|-
!align=left |Keywords
||
Adenosine Triphosphatases; Adenosine Triphosphate; Amino Acid Sequence; Bacterial Proteins; Base Sequence; DNA; DNA Damage; DNA, Superhelical; DNA, Viral; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Hydrolysis; Molecular Sequence Data; Mutation; Protein Conformation; Sequence Homology, Amino Acid; Structure-Activity Relationship; Substrate Specificity; Ultraviolet Rays; Zinc Fingers

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180990.P4f690da79ba31&page=180990&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="W4f690da7b2526" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180990.W4f690da7b2526&page=180990&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>
==Mutant alleles described in the paper==
<protect>

{| id="N4f690e6caac09" class=" tableEdit "
|-
!|Mutant!!Residue Change!!Codon Change!!UV Sensitivity

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180990.N4f690e6caac09&page=180990&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:8444906

2012-03-20T23:07:19Z

Chrischen: Fill PMID: Page!

{{RightTOC}}


{| id="P4f690da79ba31" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Wang, J and Grossman, L''' (1993) Mutations in the helix-turn-helix motif of the Escherichia coli UvrA protein eliminate its specificity for UV-damaged DNA.''J. Biol. Chem.'' '''268''':5323-31
|-
!align=left |Abstract
||
The Escherichia coli UvrA protein possesses a stretch of amino acids, 494 to 513, that matches the consensus sequence of the helix-turn-helix motif of many sequence-specific DNA binding proteins. It also has two zinc finger motif regions and two ATP binding sites. To study the potential roles of both helix-turn-helix and zinc finger motifs in the functioning of UvrA protein, random mutations were created in these motif regions by degenerate oligonucleotide-directed mutagenesis. Using this method, 12 single substitution mutants (eight in the helix-turn-helix motif region, one in the N-terminal zinc finger region, and three in the C-terminal zinc finger region) were isolated that failed to confer UV resistance in the E. coli strain deleted of the uvrA gene. One "hyper" UV-resistant mutant, G275A, was identified that conferred significantly more UV resistance than the wild type in the MH1-delta A strain. To further investigate the mechanism of failure of these mutant UvrA proteins to support nucleotide excision repair, two mutant UvrA proteins, G502D and V508D, were selected for purification and characterization, since they carry mutations at the positions offered as the putative constellation for the helix-turn-helix motif. The binding affinity of these two mutants for nonirradiated plasmid DNA was unaffected by the mutations. Both mutant proteins exhibited substantial ATPase activity, and together with the UvrB protein, they were capable of generating positively supercoiled plasmid DNA from the relaxed form in the presence of ATP and bacterial topoisomerase I. However, both mutant proteins failed to respond to UV damage in the filter binding assay and were incapable of forming 2 x SSC-resistant nucleoprotein complexes with UvrB protein on UV-irradiated plasmid DNA. Taking these properties together, it appears that the mutations in the helix-turn-helix motif region impaired the UvrA protein's ability to recognize UV damage, while its other activities were largely unaffected. Interestingly, ERCC-3, a human DNA repair protein, also has a similar helix-turn-helix motif. Given the highly conserved nature of repair proteins in general, this observation raises the possibility that both procaryotes and eucaryotes might use similar mechanisms to recognize damaged sites in their genomes.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8444906 PubMed]

|-
!align=left |Keywords
||
Adenosine Triphosphatases; Adenosine Triphosphate; Amino Acid Sequence; Bacterial Proteins; Base Sequence; DNA; DNA Damage; DNA, Superhelical; DNA, Viral; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Hydrolysis; Molecular Sequence Data; Mutation; Protein Conformation; Sequence Homology, Amino Acid; Structure-Activity Relationship; Substrate Specificity; Ultraviolet Rays; Zinc Fingers

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180990.P4f690da79ba31&page=180990&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="W4f690da7b2526" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180990.W4f690da7b2526&page=180990&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:8444906

2012-03-20T23:07:19Z

Chrischen: New PMID: Page!

uvrA:Gene

2012-03-20T22:51:12Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|uvrA}}{{Tab On|Gene|uvrA}}{{Tab|Gene Product(s)|uvrA}}{{Tab|Expression|uvrA}}{{Tab|Evolution|uvrA}}{{Tab|On One Page|uvrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="A46aa05ee4618e" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''uvrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Ultraviolet resistant
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK4050, b4058, JW4019, dar, dinE''<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.A46aa05ee4618e&page=18675&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="I46aa05eec9b28" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
92.01 minutes, 92.01 minutes 
|
MG1655: 4271894..4269072 <gbrowseImage>
name=NC_000913:4269072..4271894
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11061 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4268072|4272894|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4269072|4271894|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11061 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 4252803..4249981 <gbrowseImage>
name=NC_012967:4249981..4252803
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|4249981|4252803|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4249981|4252803|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 4207917..4210739 <gbrowseImage>
name=NC_012759:4207917..4210739
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|4207917|4210739|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4207917|4210739|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4277461..4274639 <gbrowseImage>
name=AC_000091:4274639..4277461
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|4273639|4278461|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|4274639|4277461|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.I46aa05eec9b28&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="F46aa05ef909f4" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4269072
|
Edman degradation
|
PMID:3007478 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.F46aa05ef909f4&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="O46aa05efdbaa3" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
Strain DH10B
|

|
V332L
|

|
Nonsynonomous mutation
|
PMID:18245285
|
[https://asap.ahabs.wisc.edu/asap/home.php ASAP]
|
This is a SNP in E. coli K-12 Strain [[DH10B]]
|-
|
''uvrA(del)'' (Keio:JW4019)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]

[http://cgsc2.biology.yale.edu/Strain.php?ID=109170 CGSC10889]<ref name='LIB:cgsc'/>
|

|-
|
uvrAC253A,H,S
|

|
C253A,H,S
|

|
Reduced activity
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0A698 UniProt:P0A698]
|-
|
''uvrA6''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:4736|CGSC:4736]]
|
Figure 5

Strain AB1157
|-
|
''uvrA157''
|

|

|

|

|

|
[[cgscmutation:4765|CGSC:4765]]
|

|-
|
''uvrA103''
|

|

|

|

|

|
[[cgscmutation:7745|CGSC:7745]]
|

|-
|
''uvrA155''
|

|

|

|

|

|
[[cgscmutation:8494|CGSC:8494]]
|

|-
|
''uvrA277::Tn10''
|

|

|

|

|

|
[[cgscmutation:8866|CGSC:8866]]
|

|-
|
''uvrA37''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:9254|CGSC:9254]]
|
Figure 5

Strain Hfr J2
|-
|
''uvrA14''
|

|

|

|

|

|
[[cgscmutation:9283|CGSC:9283]]
|

|-
|
''uvrA18''
|

|

|

|

|

|
[[cgscmutation:9291|CGSC:9291]]
|

|-
|
''uvrA19''
|

|

|

|

|

|
[[cgscmutation:9293|CGSC:9293]]
|

|-
|
''uvrA281''
|

|

|

|

|

|
[[cgscmutation:10632|CGSC:10632]]
|

|-
|
''uvrA154''
|

|

|

|

|

|
[[cgscmutation:11960|CGSC:11960]]
|

|-
|
''uvrA0''
|

|

|

|

|

|
[[cgscmutation:17383|CGSC:17383]]
|

|-
|
''uvrA215::Mud(Ap,lac)''
|

|

|

|

|
PMID:6771759 PMID:6780917
|
[[cgscmutation:67398|CGSC:67398]]
|

|-
|
''uvrA753(del)::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:103342|CGSC:103342]]
|

|-
|
''uvrA(del)::Tn10''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.
|
PMID:17040104
|
Experimental strain:GY5564
|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.
|
PMID:17040104
|

|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.
|
PMID:16970815
|

|
figure 2
|-
|
''uvrA'' K689A
|

|
Lysine to Alanine
|
change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680E
|

|
Lysine to Glutamic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691A
|

|
Arginine to Alanine
|
Change in DNA binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691D
|

|
Arginine to Aspartic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680A R691A
|

|
Lysine to Alanine & Arginine to Alanine
|
Change in binding affinity
|
Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
DNA repair
|
Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.
|
PMID:17040104
|

|
Figure 4
|-
|
''uvrAB'' K45A
|

|
Lysine to alanine
|
change in enzyme activity
|
ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.
|
PMID:17630776
|

|

|-
|
''ZnG uvrA''
|

|
11 amino acid change, figure 1.
|
DNA binding
|
Mutant shows decreased binding affinity to damaged DNA, table 1.
|
PMID:16829526
|

|

|-
|
''uvrA'' G502D V508D
|

|
Glycine to Aspartic Acid & Valine to Aspartic Acid
|
Sensitivity to
|
Mutation decreases UV-radiation resistance by causing the loss of uvrA's binding specificity between damaged and undamaged DNA. Figure 7.
|
PMID:8444906
|

|
Figure 7

Parent Strain: MH1-∆A
|-
|
''uvrA'' G275A
|

|
Glycine to Alanine
|
Resistant to
|
Mutation creates "hyper" UV-resistant phenotype greater than wild-type's UV-resistance, figure 4.
|
PMID:8444906
|

|
Figure 4

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.O46aa05efdbaa3&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="S4e04c56173535" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18675.S4e04c56173535&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="L46aa05f0a24e1" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW4019
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG
|-
|
[[:Category:Kohara_Phage:12D1|12D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=637 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3F12|3F12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=638 Genobase]
|
PMID:3038334
|-
|
[[malF:Gene|malF3089::Tn10]]
|
Linked marker
|
CAG12164 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40371 CGSC7476]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[yjcS:Gene|zje-2241::Tn10]]
|
Linked marker
|
CAG18427 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40603 CGSC7481]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46aa05f0a24e1&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="X46aa05f14ff9b" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948559 GeneID:948559]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.X46aa05f14ff9b&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="L46a71f2747b21" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46a71f2747b21&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:uvr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

uvrA:Gene

2012-03-20T22:49:37Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|uvrA}}{{Tab On|Gene|uvrA}}{{Tab|Gene Product(s)|uvrA}}{{Tab|Expression|uvrA}}{{Tab|Evolution|uvrA}}{{Tab|On One Page|uvrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="A46aa05ee4618e" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''uvrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Ultraviolet resistant
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK4050, b4058, JW4019, dar, dinE''<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.A46aa05ee4618e&page=18675&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="I46aa05eec9b28" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
92.01 minutes, 92.01 minutes 
|
MG1655: 4271894..4269072 <gbrowseImage>
name=NC_000913:4269072..4271894
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11061 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4268072|4272894|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4269072|4271894|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11061 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 4252803..4249981 <gbrowseImage>
name=NC_012967:4249981..4252803
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|4249981|4252803|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4249981|4252803|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 4207917..4210739 <gbrowseImage>
name=NC_012759:4207917..4210739
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|4207917|4210739|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4207917|4210739|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4277461..4274639 <gbrowseImage>
name=AC_000091:4274639..4277461
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|4273639|4278461|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|4274639|4277461|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.I46aa05eec9b28&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="F46aa05ef909f4" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4269072
|
Edman degradation
|
PMID:3007478 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.F46aa05ef909f4&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="O46aa05efdbaa3" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
Strain DH10B
|

|
V332L
|

|
Nonsynonomous mutation
|
PMID:18245285
|
[https://asap.ahabs.wisc.edu/asap/home.php ASAP]
|
This is a SNP in E. coli K-12 Strain [[DH10B]]
|-
|
''uvrA(del)'' (Keio:JW4019)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]

[http://cgsc2.biology.yale.edu/Strain.php?ID=109170 CGSC10889]<ref name='LIB:cgsc'/>
|

|-
|
uvrAC253A,H,S
|

|
C253A,H,S
|

|
Reduced activity
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0A698 UniProt:P0A698]
|-
|
''uvrA6''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:4736|CGSC:4736]]
|
Figure 5

Strain AB1157
|-
|
''uvrA157''
|

|

|

|

|

|
[[cgscmutation:4765|CGSC:4765]]
|

|-
|
''uvrA103''
|

|

|

|

|

|
[[cgscmutation:7745|CGSC:7745]]
|

|-
|
''uvrA155''
|

|

|

|

|

|
[[cgscmutation:8494|CGSC:8494]]
|

|-
|
''uvrA277::Tn10''
|

|

|

|

|

|
[[cgscmutation:8866|CGSC:8866]]
|

|-
|
''uvrA37''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:9254|CGSC:9254]]
|
Figure 5

Strain Hfr J2
|-
|
''uvrA14''
|

|

|

|

|

|
[[cgscmutation:9283|CGSC:9283]]
|

|-
|
''uvrA18''
|

|

|

|

|

|
[[cgscmutation:9291|CGSC:9291]]
|

|-
|
''uvrA19''
|

|

|

|

|

|
[[cgscmutation:9293|CGSC:9293]]
|

|-
|
''uvrA281''
|

|

|

|

|

|
[[cgscmutation:10632|CGSC:10632]]
|

|-
|
''uvrA154''
|

|

|

|

|

|
[[cgscmutation:11960|CGSC:11960]]
|

|-
|
''uvrA0''
|

|

|

|

|

|
[[cgscmutation:17383|CGSC:17383]]
|

|-
|
''uvrA215::Mud(Ap,lac)''
|

|

|

|

|
PMID:6771759 PMID:6780917
|
[[cgscmutation:67398|CGSC:67398]]
|

|-
|
''uvrA753(del)::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:103342|CGSC:103342]]
|

|-
|
''uvrA(del)::Tn10''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.
|
PMID:17040104
|
Experimental strain:GY5564
|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.
|
PMID:17040104
|

|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.
|
PMID:16970815
|

|
figure 2
|-
|
''uvrA'' K689A
|

|
Lysine to Alanine
|
change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680E
|

|
Lysine to Glutamic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691A
|

|
Arginine to Alanine
|
Change in DNA binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691D
|

|
Arginine to Aspartic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680A R691A
|

|
Lysine to Alanine & Arginine to Alanine
|
Change in binding affinity
|
Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
DNA repair
|
Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.
|
PMID:17040104
|

|
Figure 4
|-
|
''uvrAB'' K45A
|

|
Lysine to alanine
|
change in enzyme activity
|
ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.
|
PMID:17630776
|

|

|-
|
''ZnG uvrA''
|

|
11 amino acid change, figure 1.
|
DNA binding
|
Mutant shows decreased binding affinity to damaged DNA, table 1.
|
PMID:16829526
|

|

|-
|
''uvrA'' G502D V508D
|

|
Glycine to Aspartic Acid & Valine to Aspartic Acid
|
Sensitivity to
|
Mutation decreases UV-radiation resistance by causing the loss of uvrA's binding specificity between damaged and undamaged DNA. Figure 7.
|
PMID:8444906
|

|
Figure 7

Strain: MH1-∆A
|-
|
''uvrA'' G275A
|

|
Glycine to Alanine
|
Resistant to
|
Mutation creates "hyper" UV-resistant phenotype greater than MH1-∆A wild-type strain, figure 4.
|
PMID:8444906
|

|
Figure 4

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.O46aa05efdbaa3&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="S4e04c56173535" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18675.S4e04c56173535&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="L46aa05f0a24e1" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW4019
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG
|-
|
[[:Category:Kohara_Phage:12D1|12D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=637 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3F12|3F12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=638 Genobase]
|
PMID:3038334
|-
|
[[malF:Gene|malF3089::Tn10]]
|
Linked marker
|
CAG12164 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40371 CGSC7476]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[yjcS:Gene|zje-2241::Tn10]]
|
Linked marker
|
CAG18427 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40603 CGSC7481]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46aa05f0a24e1&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="X46aa05f14ff9b" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948559 GeneID:948559]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.X46aa05f14ff9b&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="L46a71f2747b21" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46a71f2747b21&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:uvr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

uvrA:Gene

2012-03-20T22:37:46Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|uvrA}}{{Tab On|Gene|uvrA}}{{Tab|Gene Product(s)|uvrA}}{{Tab|Expression|uvrA}}{{Tab|Evolution|uvrA}}{{Tab|On One Page|uvrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="A46aa05ee4618e" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''uvrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Ultraviolet resistant
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK4050, b4058, JW4019, dar, dinE''<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.A46aa05ee4618e&page=18675&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="I46aa05eec9b28" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
92.01 minutes, 92.01 minutes 
|
MG1655: 4271894..4269072 <gbrowseImage>
name=NC_000913:4269072..4271894
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11061 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4268072|4272894|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4269072|4271894|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11061 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 4252803..4249981 <gbrowseImage>
name=NC_012967:4249981..4252803
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|4249981|4252803|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4249981|4252803|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 4207917..4210739 <gbrowseImage>
name=NC_012759:4207917..4210739
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|4207917|4210739|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4207917|4210739|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4277461..4274639 <gbrowseImage>
name=AC_000091:4274639..4277461
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|4273639|4278461|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|4274639|4277461|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.I46aa05eec9b28&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="F46aa05ef909f4" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4269072
|
Edman degradation
|
PMID:3007478 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.F46aa05ef909f4&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="O46aa05efdbaa3" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
Strain DH10B
|

|
V332L
|

|
Nonsynonomous mutation
|
PMID:18245285
|
[https://asap.ahabs.wisc.edu/asap/home.php ASAP]
|
This is a SNP in E. coli K-12 Strain [[DH10B]]
|-
|
''uvrA(del)'' (Keio:JW4019)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]

[http://cgsc2.biology.yale.edu/Strain.php?ID=109170 CGSC10889]<ref name='LIB:cgsc'/>
|

|-
|
uvrAC253A,H,S
|

|
C253A,H,S
|

|
Reduced activity
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0A698 UniProt:P0A698]
|-
|
''uvrA6''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:4736|CGSC:4736]]
|
Figure 5

Strain AB1157
|-
|
''uvrA157''
|

|

|

|

|

|
[[cgscmutation:4765|CGSC:4765]]
|

|-
|
''uvrA103''
|

|

|

|

|

|
[[cgscmutation:7745|CGSC:7745]]
|

|-
|
''uvrA155''
|

|

|

|

|

|
[[cgscmutation:8494|CGSC:8494]]
|

|-
|
''uvrA277::Tn10''
|

|

|

|

|

|
[[cgscmutation:8866|CGSC:8866]]
|

|-
|
''uvrA37''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:9254|CGSC:9254]]
|
Figure 5

Strain Hfr J2
|-
|
''uvrA14''
|

|

|

|

|

|
[[cgscmutation:9283|CGSC:9283]]
|

|-
|
''uvrA18''
|

|

|

|

|

|
[[cgscmutation:9291|CGSC:9291]]
|

|-
|
''uvrA19''
|

|

|

|

|

|
[[cgscmutation:9293|CGSC:9293]]
|

|-
|
''uvrA281''
|

|

|

|

|

|
[[cgscmutation:10632|CGSC:10632]]
|

|-
|
''uvrA154''
|

|

|

|

|

|
[[cgscmutation:11960|CGSC:11960]]
|

|-
|
''uvrA0''
|

|

|

|

|

|
[[cgscmutation:17383|CGSC:17383]]
|

|-
|
''uvrA215::Mud(Ap,lac)''
|

|

|

|

|
PMID:6771759 PMID:6780917
|
[[cgscmutation:67398|CGSC:67398]]
|

|-
|
''uvrA753(del)::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:103342|CGSC:103342]]
|

|-
|
''uvrA(del)::Tn10''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.
|
PMID:17040104
|
Experimental strain:GY5564
|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.
|
PMID:17040104
|

|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.
|
PMID:16970815
|

|
figure 2
|-
|
''uvrA'' K689A
|

|
Lysine to Alanine
|
change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680E
|

|
Lysine to Glutamic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691A
|

|
Arginine to Alanine
|
Change in DNA binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691D
|

|
Arginine to Aspartic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680A R691A
|

|
Lysine to Alanine & Arginine to Alanine
|
Change in binding affinity
|
Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
DNA repair
|
Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.
|
PMID:17040104
|

|
Figure 4
|-
|
''uvrAB'' K45A
|

|
Lysine to alanine
|
change in enzyme activity
|
ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.
|
PMID:17630776
|

|

|-
|
''ZnG uvrA''
|

|
11 amino acid change, figure 1.
|
DNA binding
|
Mutant shows decreased binding affinity to damaged DNA, table 1.
|
PMID:16829526
|

|

|-
|
''uvrA'' G502D V508D
|

|
Glycine to Aspartic Acid & Valine to Aspartic Acid
|
Sensitivity to
|
Mutation decreases UV-radiation resistance by causing the loss of uvrA's binding specificity between damaged and undamaged DNA. Figure 7.
|
PMID:8444906
|

|
Figure 7

Parent Strain: MH1-∆A

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.O46aa05efdbaa3&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="S4e04c56173535" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18675.S4e04c56173535&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="L46aa05f0a24e1" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW4019
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG
|-
|
[[:Category:Kohara_Phage:12D1|12D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=637 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3F12|3F12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=638 Genobase]
|
PMID:3038334
|-
|
[[malF:Gene|malF3089::Tn10]]
|
Linked marker
|
CAG12164 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40371 CGSC7476]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[yjcS:Gene|zje-2241::Tn10]]
|
Linked marker
|
CAG18427 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40603 CGSC7481]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46aa05f0a24e1&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="X46aa05f14ff9b" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948559 GeneID:948559]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.X46aa05f14ff9b&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="L46a71f2747b21" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46a71f2747b21&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:uvr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

uvrA:Gene

2012-03-08T01:00:39Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|uvrA}}{{Tab On|Gene|uvrA}}{{Tab|Gene Product(s)|uvrA}}{{Tab|Expression|uvrA}}{{Tab|Evolution|uvrA}}{{Tab|On One Page|uvrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="A46aa05ee4618e" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''uvrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Ultraviolet resistant
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK4050, b4058, JW4019, dar, dinE''<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.A46aa05ee4618e&page=18675&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="I46aa05eec9b28" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
92.01 minutes, 92.01 minutes 
|
MG1655: 4271894..4269072 <gbrowseImage>
name=NC_000913:4269072..4271894
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11061 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4268072|4272894|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4269072|4271894|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11061 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 4252803..4249981 <gbrowseImage>
name=NC_012967:4249981..4252803
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|4249981|4252803|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4249981|4252803|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 4207917..4210739 <gbrowseImage>
name=NC_012759:4207917..4210739
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|4207917|4210739|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4207917|4210739|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4277461..4274639 <gbrowseImage>
name=AC_000091:4274639..4277461
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|4273639|4278461|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|4274639|4277461|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.I46aa05eec9b28&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="F46aa05ef909f4" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4269072
|
Edman degradation
|
PMID:3007478 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.F46aa05ef909f4&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="O46aa05efdbaa3" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
Strain DH10B
|

|
V332L
|

|
Nonsynonomous mutation
|
PMID:18245285
|
[https://asap.ahabs.wisc.edu/asap/home.php ASAP]
|
This is a SNP in E. coli K-12 Strain [[DH10B]]
|-
|
''uvrA(del)'' (Keio:JW4019)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]

[http://cgsc2.biology.yale.edu/Strain.php?ID=109170 CGSC10889]<ref name='LIB:cgsc'/>
|

|-
|
uvrAC253A,H,S
|

|
C253A,H,S
|

|
Reduced activity
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0A698 UniProt:P0A698]
|-
|
''uvrA6''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:4736|CGSC:4736]]
|
Figure 5

Strain AB1157
|-
|
''uvrA157''
|

|

|

|

|

|
[[cgscmutation:4765|CGSC:4765]]
|

|-
|
''uvrA103''
|

|

|

|

|

|
[[cgscmutation:7745|CGSC:7745]]
|

|-
|
''uvrA155''
|

|

|

|

|

|
[[cgscmutation:8494|CGSC:8494]]
|

|-
|
''uvrA277::Tn10''
|

|

|

|

|

|
[[cgscmutation:8866|CGSC:8866]]
|

|-
|
''uvrA37''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:9254|CGSC:9254]]
|
Figure 5

Strain Hfr J2
|-
|
''uvrA14''
|

|

|

|

|

|
[[cgscmutation:9283|CGSC:9283]]
|

|-
|
''uvrA18''
|

|

|

|

|

|
[[cgscmutation:9291|CGSC:9291]]
|

|-
|
''uvrA19''
|

|

|

|

|

|
[[cgscmutation:9293|CGSC:9293]]
|

|-
|
''uvrA281''
|

|

|

|

|

|
[[cgscmutation:10632|CGSC:10632]]
|

|-
|
''uvrA154''
|

|

|

|

|

|
[[cgscmutation:11960|CGSC:11960]]
|

|-
|
''uvrA0''
|

|

|

|

|

|
[[cgscmutation:17383|CGSC:17383]]
|

|-
|
''uvrA215::Mud(Ap,lac)''
|

|

|

|

|
PMID:6771759 PMID:6780917
|
[[cgscmutation:67398|CGSC:67398]]
|

|-
|
''uvrA753(del)::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:103342|CGSC:103342]]
|

|-
|
''uvrA(del)::Tn10''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.
|
PMID:17040104
|
Experimental strain:GY5564
|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.
|
PMID:17040104
|

|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.
|
PMID:16970815
|

|
figure 2
|-
|
''uvrA'' K689A
|

|
Lysine to Alanine
|
change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680E
|

|
Lysine to Glutamic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691A
|

|
Arginine to Alanine
|
Change in DNA binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691D
|

|
Arginine to Aspartic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680A R691A
|

|
Lysine to Alanine & Arginine to Alanine
|
Change in binding affinity
|
Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
DNA repair
|
Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.
|
PMID:17040104
|

|
Figure 4
|-
|
''uvrAB'' K45A
|

|
Lysine to alanine
|
change in enzyme activity
|
ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.
|
PMID:17630776
|

|

|-
|
''ZnG uvrA''
|

|
11 amino acid change, figure 1.
|
DNA binding
|
Mutant shows decreased binding affinity to damaged DNA, table 1.
|
PMID:16829526
|

|

|-
|
''uvrA'' G502D V508D
|

|
Glycine to Aspartic Acid & Valine to Aspartic Acid
|
Sensitivity to
|
Mutation decreases UV-radiation resistance by causing the loss of uvrA's binding specificity between damaged and undamaged DNA. Figure 7.
|
PMID:8444906
|

|
Figure 7

Strain: MH1-∆A

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.O46aa05efdbaa3&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="S4e04c56173535" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18675.S4e04c56173535&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="L46aa05f0a24e1" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW4019
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG
|-
|
[[:Category:Kohara_Phage:12D1|12D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=637 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3F12|3F12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=638 Genobase]
|
PMID:3038334
|-
|
[[malF:Gene|malF3089::Tn10]]
|
Linked marker
|
CAG12164 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40371 CGSC7476]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[yjcS:Gene|zje-2241::Tn10]]
|
Linked marker
|
CAG18427 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40603 CGSC7481]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46aa05f0a24e1&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="X46aa05f14ff9b" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948559 GeneID:948559]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.X46aa05f14ff9b&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="L46a71f2747b21" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46a71f2747b21&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:uvr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

uvrA:Gene

2012-03-08T00:56:25Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|uvrA}}{{Tab On|Gene|uvrA}}{{Tab|Gene Product(s)|uvrA}}{{Tab|Expression|uvrA}}{{Tab|Evolution|uvrA}}{{Tab|On One Page|uvrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="A46aa05ee4618e" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''uvrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Ultraviolet resistant
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK4050, b4058, JW4019, dar, dinE''<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.A46aa05ee4618e&page=18675&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="I46aa05eec9b28" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
92.01 minutes, 92.01 minutes 
|
MG1655: 4271894..4269072 <gbrowseImage>
name=NC_000913:4269072..4271894
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11061 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4268072|4272894|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4269072|4271894|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11061 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 4252803..4249981 <gbrowseImage>
name=NC_012967:4249981..4252803
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|4249981|4252803|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4249981|4252803|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 4207917..4210739 <gbrowseImage>
name=NC_012759:4207917..4210739
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|4207917|4210739|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4207917|4210739|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4277461..4274639 <gbrowseImage>
name=AC_000091:4274639..4277461
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|4273639|4278461|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|4274639|4277461|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.I46aa05eec9b28&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="F46aa05ef909f4" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4269072
|
Edman degradation
|
PMID:3007478 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.F46aa05ef909f4&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="O46aa05efdbaa3" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
Strain DH10B
|

|
V332L
|

|
Nonsynonomous mutation
|
PMID:18245285
|
[https://asap.ahabs.wisc.edu/asap/home.php ASAP]
|
This is a SNP in E. coli K-12 Strain [[DH10B]]
|-
|
''uvrA(del)'' (Keio:JW4019)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]

[http://cgsc2.biology.yale.edu/Strain.php?ID=109170 CGSC10889]<ref name='LIB:cgsc'/>
|

|-
|
uvrAC253A,H,S
|

|
C253A,H,S
|

|
Reduced activity
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0A698 UniProt:P0A698]
|-
|
''uvrA6''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:4736|CGSC:4736]]
|
Figure 5

Strain AB1157
|-
|
''uvrA157''
|

|

|

|

|

|
[[cgscmutation:4765|CGSC:4765]]
|

|-
|
''uvrA103''
|

|

|

|

|

|
[[cgscmutation:7745|CGSC:7745]]
|

|-
|
''uvrA155''
|

|

|

|

|

|
[[cgscmutation:8494|CGSC:8494]]
|

|-
|
''uvrA277::Tn10''
|

|

|

|

|

|
[[cgscmutation:8866|CGSC:8866]]
|

|-
|
''uvrA37''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:9254|CGSC:9254]]
|
Figure 5

Strain Hfr J2
|-
|
''uvrA14''
|

|

|

|

|

|
[[cgscmutation:9283|CGSC:9283]]
|

|-
|
''uvrA18''
|

|

|

|

|

|
[[cgscmutation:9291|CGSC:9291]]
|

|-
|
''uvrA19''
|

|

|

|

|

|
[[cgscmutation:9293|CGSC:9293]]
|

|-
|
''uvrA281''
|

|

|

|

|

|
[[cgscmutation:10632|CGSC:10632]]
|

|-
|
''uvrA154''
|

|

|

|

|

|
[[cgscmutation:11960|CGSC:11960]]
|

|-
|
''uvrA0''
|

|

|

|

|

|
[[cgscmutation:17383|CGSC:17383]]
|

|-
|
''uvrA215::Mud(Ap,lac)''
|

|

|

|

|
PMID:6771759 PMID:6780917
|
[[cgscmutation:67398|CGSC:67398]]
|

|-
|
''uvrA753(del)::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:103342|CGSC:103342]]
|

|-
|
''uvrA(del)::Tn10''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.
|
PMID:17040104
|
Experimental strain:GY5564
|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.
|
PMID:17040104
|

|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.
|
PMID:16970815
|

|
figure 2
|-
|
''uvrA'' K689A
|

|
Lysine to Alanine
|
change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680E
|

|
Lysine to Glutamic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691A
|

|
Arginine to Alanine
|
Change in DNA binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691D
|

|
Arginine to Aspartic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680A R691A
|

|
Lysine to Alanine & Arginine to Alanine
|
Change in binding affinity
|
Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
DNA repair
|
Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.
|
PMID:17040104
|

|
Figure 4
|-
|
''uvrAB'' K45A
|

|
Lysine to alanine
|
change in enzyme activity
|
ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.
|
PMID:17630776
|

|

|-
|
''ZnG uvrA''
|

|
11 amino acid change, figure 1.
|
DNA binding
|
Mutant shows decreased binding affinity to damaged DNA, table 1.
|
PMID:16829526
|

|

|-
|
''uvrA'' G502D V508D
|

|
Glycine to Aspartic Acid & Valine to Aspartic Acid
|
Sensitivity to
|
Mutation decreases UV-radiation resistance by causing the loss of uvrA's binding specificity between damaged and undamaged DNA. Figure 7.
|
PMID:8444906
|

|
Figure 7

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.O46aa05efdbaa3&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="S4e04c56173535" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18675.S4e04c56173535&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="L46aa05f0a24e1" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW4019
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG
|-
|
[[:Category:Kohara_Phage:12D1|12D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=637 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3F12|3F12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=638 Genobase]
|
PMID:3038334
|-
|
[[malF:Gene|malF3089::Tn10]]
|
Linked marker
|
CAG12164 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40371 CGSC7476]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[yjcS:Gene|zje-2241::Tn10]]
|
Linked marker
|
CAG18427 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40603 CGSC7481]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46aa05f0a24e1&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="X46aa05f14ff9b" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948559 GeneID:948559]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.X46aa05f14ff9b&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="L46a71f2747b21" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46a71f2747b21&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:uvr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

uvrA:Gene

2012-02-17T22:50:12Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|uvrA}}{{Tab On|Gene|uvrA}}{{Tab|Gene Product(s)|uvrA}}{{Tab|Expression|uvrA}}{{Tab|Evolution|uvrA}}{{Tab|On One Page|uvrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="A46aa05ee4618e" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''uvrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Ultraviolet resistant
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK4050, b4058, JW4019, dar, dinE''<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.A46aa05ee4618e&page=18675&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="I46aa05eec9b28" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
92.01 minutes, 92.01 minutes 
|
MG1655: 4271894..4269072 <gbrowseImage>
name=NC_000913:4269072..4271894
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11061 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4268072|4272894|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4269072|4271894|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11061 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 4252803..4249981 <gbrowseImage>
name=NC_012967:4249981..4252803
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|4249981|4252803|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4249981|4252803|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 4207917..4210739 <gbrowseImage>
name=NC_012759:4207917..4210739
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|4207917|4210739|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4207917|4210739|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4277461..4274639 <gbrowseImage>
name=AC_000091:4274639..4277461
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|4273639|4278461|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|4274639|4277461|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.I46aa05eec9b28&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="F46aa05ef909f4" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4269072
|
Edman degradation
|
PMID:3007478 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.F46aa05ef909f4&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="O46aa05efdbaa3" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
Strain DH10B
|

|
V332L
|

|
Nonsynonomous mutation
|
PMID:18245285
|
[https://asap.ahabs.wisc.edu/asap/home.php ASAP]
|
This is a SNP in E. coli K-12 Strain [[DH10B]]
|-
|
''uvrA(del)'' (Keio:JW4019)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]

[http://cgsc2.biology.yale.edu/Strain.php?ID=109170 CGSC10889]<ref name='LIB:cgsc'/>
|

|-
|
uvrAC253A,H,S
|

|
C253A,H,S
|

|
Reduced activity
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0A698 UniProt:P0A698]
|-
|
''uvrA6''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:4736|CGSC:4736]]
|
Figure 5

Strain AB1157
|-
|
''uvrA157''
|

|

|

|

|

|
[[cgscmutation:4765|CGSC:4765]]
|

|-
|
''uvrA103''
|

|

|

|

|

|
[[cgscmutation:7745|CGSC:7745]]
|

|-
|
''uvrA155''
|

|

|

|

|

|
[[cgscmutation:8494|CGSC:8494]]
|

|-
|
''uvrA277::Tn10''
|

|

|

|

|

|
[[cgscmutation:8866|CGSC:8866]]
|

|-
|
''uvrA37''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to UV-radiation
|
PMID:5335128
|
[[cgscmutation:9254|CGSC:9254]]
|
Figure 5

Strain Hfr J2
|-
|
''uvrA14''
|

|

|

|

|

|
[[cgscmutation:9283|CGSC:9283]]
|

|-
|
''uvrA18''
|

|

|

|

|

|
[[cgscmutation:9291|CGSC:9291]]
|

|-
|
''uvrA19''
|

|

|

|

|

|
[[cgscmutation:9293|CGSC:9293]]
|

|-
|
''uvrA281''
|

|

|

|

|

|
[[cgscmutation:10632|CGSC:10632]]
|

|-
|
''uvrA154''
|

|

|

|

|

|
[[cgscmutation:11960|CGSC:11960]]
|

|-
|
''uvrA0''
|

|

|

|

|

|
[[cgscmutation:17383|CGSC:17383]]
|

|-
|
''uvrA215::Mud(Ap,lac)''
|

|

|

|

|
PMID:6771759 PMID:6780917
|
[[cgscmutation:67398|CGSC:67398]]
|

|-
|
''uvrA753(del)::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:103342|CGSC:103342]]
|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.
|
PMID:17040104
|
Experimental strain:GY5564
|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.
|
PMID:17040104
|

|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.
|
PMID:16970815
|

|
figure 2
|-
|
''uvrA'' K689A
|

|
Lysine to Alanine
|
change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680E
|

|
Lysine to Glutamic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691A
|

|
Arginine to Alanine
|
Change in DNA binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691D
|

|
Arginine to Aspartic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680A R691A
|

|
Lysine to Alanine & Arginine to Alanine
|
Change in binding affinity
|
Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.
|
PMID:17040104
|

|
Figure 4
|-
|
''uvrAB'' K45A
|

|
Lysine to alanine
|
change in enzyme activity
|
ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.
|
PMID:17630776
|

|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.O46aa05efdbaa3&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="S4e04c56173535" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18675.S4e04c56173535&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="L46aa05f0a24e1" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW4019
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG
|-
|
[[:Category:Kohara_Phage:12D1|12D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=637 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3F12|3F12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=638 Genobase]
|
PMID:3038334
|-
|
[[malF:Gene|malF3089::Tn10]]
|
Linked marker
|
CAG12164 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40371 CGSC7476]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[yjcS:Gene|zje-2241::Tn10]]
|
Linked marker
|
CAG18427 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40603 CGSC7481]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46aa05f0a24e1&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="X46aa05f14ff9b" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948559 GeneID:948559]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.X46aa05f14ff9b&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
{{BacktoTop}}


{| id="L46a71f2747b21" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46a71f2747b21&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


<noinclude><protect>
==References==
{{BacktoTop}}
{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:uvr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

uvrA:Gene

2012-02-17T22:37:17Z

Chrischen: Table edited by Chrischen via TableEdit

{{#css:Suppresslinks.css}}<css>{{:Suppresslinks.css}}</css>
<noinclude>{{TabsTop}}
{{Tab|Quickview|uvrA}}{{Tab On|Gene|uvrA}}{{Tab|Gene Product(s)|uvrA}}{{Tab|Expression|uvrA}}{{Tab|Evolution|uvrA}}{{Tab|On One Page|uvrA}}{{TabsBottom}}
{|
|- style="background-color:white; border:solid 2px #CCFFFF; text-align:center; white-space: nowrap;"
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Links |Links]]
|{{Sub}}[[#References |References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
</noinclude><protect>
==Nomenclature==
{{BacktoTop}}

{{Gene_nomenclature_help}}
{| id="A46aa05ee4618e" class=" tableEdit Gene_nomenclature_table"

|-
!align=left {{table_heading_style}} |Standard name
||
''uvrA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Ultraviolet resistant
|-
!align=left {{table_heading_style}} |Synonyms
||
''ECK4050, b4058, JW4019, dar, dinE''<ref name='LIB:Riley_2006'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.A46aa05ee4618e&page=18675&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table] ||
|}


</protect>
===Notes===

==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>

{{Gene_location_help}}
{| id="I46aa05eec9b28" class=" tableEdit Gene_location_table"
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|
MG1655
|
92.01 minutes, 92.01 minutes 
|
MG1655: 4271894..4269072 <gbrowseImage>
name=NC_000913:4269072..4271894
source=MG1655
preset=GeneLocation
</gbrowseImage>

|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11061 EcoCyc]
{{GBrowseLink|MG1655|NC_000913|4268072|4272894|GBrowse(MG1655)}}

|
{{GBrowseFastaLink|MG1655|NC_000913|4269072|4271894|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11061 EcoCyc Sequence Dump]
|-
|
[[REL606]]
|

|
NC_012967: 4252803..4249981 <gbrowseImage>
name=NC_012967:4249981..4252803
source=REL606
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|REL606|NC_012967|4249981|4252803|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4249981|4252803|FASTA Dump}}
|-
|
[[BW2952]]
|

|
NC_012759: 4207917..4210739 <gbrowseImage>
name=NC_012759:4207917..4210739
source=BW2952
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|BW2952|NC_012759|4207917|4210739|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4207917|4210739|FASTA Dump}}
|-
|
W3110
|
 
|
W3110: 4277461..4274639 <gbrowseImage>
name=AC_000091:4274639..4277461
source=W3110
preset=GeneLocation
</gbrowseImage>

|
{{GBrowseLink|W3110|AC_000091|4273639|4278461|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|AC_000091|4274639|4277461|FASTA Dump}}

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.I46aa05eec9b28&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table] || || || ||
|}


</protect>
===Notes===

==Sequence Features==
{{BacktoTop}}

{{Gene_sequence_features_help}}
{| id="F46aa05ef909f4" class=" tableEdit Gene_sequence_features_table"
|- {{table_heading_style}}
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4269072
|
Edman degradation
|
PMID:3007478 
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.F46aa05ef909f4&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table] || || || || ||
|}


<protect></protect>
====Notes====

==Alleles and Phenotypes==
{{BacktoTop}}

{{Gene_alleles_help}}
{| {{Prettytable}} id="O46aa05efdbaa3" class=" tableEdit Gene_allele_table dataTable"
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
Strain DH10B
|

|
V332L
|

|
Nonsynonomous mutation
|
PMID:18245285
|
[https://asap.ahabs.wisc.edu/asap/home.php ASAP]
|
This is a SNP in E. coli K-12 Strain [[DH10B]]
|-
|
''uvrA(del)'' (Keio:JW4019)
|
deletion
|
deletion
|

|

|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]

[http://cgsc2.biology.yale.edu/Strain.php?ID=109170 CGSC10889]<ref name='LIB:cgsc'/>
|

|-
|
uvrAC253A,H,S
|

|
C253A,H,S
|

|
Reduced activity
|

|

|
seeded from [http://www.uniprot.org/uniprot/P0A698 UniProt:P0A698]
|-
|
''uvrA6''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to DNA damage from UV-radiation
|
PMID:5335128
|
[[cgscmutation:4736|CGSC:4736]]
|
Figure 5

Origin is Hfr 313 X AB1157
|-
|
''uvrA157''
|

|

|

|

|

|
[[cgscmutation:4765|CGSC:4765]]
|

|-
|
''uvrA103''
|

|

|

|

|

|
[[cgscmutation:7745|CGSC:7745]]
|

|-
|
''uvrA155''
|

|

|

|

|

|
[[cgscmutation:8494|CGSC:8494]]
|

|-
|
''uvrA277::Tn10''
|

|

|

|

|

|
[[cgscmutation:8866|CGSC:8866]]
|

|-
|
''uvrA37''
|

|

|
Sensitivity to
|
Mutation increases sensitivity to DNA damage from UV-radiation
|
PMID:5335128
|
[[cgscmutation:9254|CGSC:9254]]
|
Figure 5

Origin is Hfr J2
|-
|
''uvrA14''
|

|

|

|

|

|
[[cgscmutation:9283|CGSC:9283]]
|

|-
|
''uvrA18''
|

|

|

|

|

|
[[cgscmutation:9291|CGSC:9291]]
|

|-
|
''uvrA19''
|

|

|

|

|

|
[[cgscmutation:9293|CGSC:9293]]
|

|-
|
''uvrA281''
|

|

|

|

|

|
[[cgscmutation:10632|CGSC:10632]]
|

|-
|
''uvrA154''
|

|

|

|

|

|
[[cgscmutation:11960|CGSC:11960]]
|

|-
|
''uvrA0''
|

|

|

|

|

|
[[cgscmutation:17383|CGSC:17383]]
|

|-
|
''uvrA215::Mud(Ap,lac)''
|

|

|

|

|
PMID:6771759 PMID:6780917
|
[[cgscmutation:67398|CGSC:67398]]
|

|-
|
''uvrA753(del)::kan''
|

|

|

|

|
PMID:16738554
|
[[cgscmutation:103342|CGSC:103342]]
|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, fig 1 & 2.
|
PMID:17040104
|
Experimental strain:GY5564
|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation increased sensitivity to mitomycin C but at a slower rate than single deletions, figure 1.
|
PMID:17040104
|

|

|-
|
''uvrA(del)''
|
deletion
|
deletion
|
Sensitivity to
|
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2.
|
PMID:16970815
|

|
figure 2
|-
|
''uvrA'' K689A
|

|
Lysine to Alanine
|
change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680E
|

|
Lysine to Glutamic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691A
|

|
Arginine to Alanine
|
Change in DNA binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' R691D
|

|
Arginine to Aspartic Acid
|
Change in binding affinity
|
Mutation caused a decrease in binding affinity to DNA, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA'' K680A R691A
|

|
Lysine to Alanine & Arginine to Alanine
|
Change in binding affinity
|
Double mutation caused a decrease in binding affinity to DNA more than single point mutations, table 1.
|
PMID:18248777
|

|

|-
|
''uvrA::Tn10 uvrC34''
|

|

|
Sensitivity to
|
Double mutation was able to recover high molecular weight DNA, though at a much slower rate than wild type.
|
PMID:17040104
|

|
Figure 4
|-
|
''uvrAB'' K45A
|

|
Lysine to alanine
|
change in enzyme activity
|
ATP hydrolysis of uvrB by ATPase is inhibited (but not of uvrA in the complex). Table 1.
|
PMID:17630776
|

|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.O46aa05efdbaa3&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table] || || || || || || ||
|}


<protect></protect>
===Notes===

==Genetic Interactions==
{{BacktoTop}}
<protect>
{{Genetic_interaction_help}}
{| id="S4e04c56173535" class=" tableEdit Genetic_interaction_table dataTable"
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18675.S4e04c56173535&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table] || || || || || ||
|}
</protect>


===Notes===

==Genetic Resources==
{{BacktoTop}}

{{Gene_resources_help}}
{| id="L46aa05f0a24e1" class=" tableEdit Gene_resource_table"
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
|
JW4019
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691

Status:Clone OK

Primer 1:GCCGATAAGATCGAAGTTCGGGG

Primer 2:CCaAGCATCGGCTTAAGGAAGCG
|-
|
[[:Category:Kohara_Phage:12D1|12D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=637 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3F12|3F12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=638 Genobase]
|
PMID:3038334
|-
|
[[malF:Gene|malF3089::Tn10]]
|
Linked marker
|
CAG12164 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40371 CGSC7476]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
[[yjcS:Gene|zje-2241::Tn10]]
|
Linked marker
|
CAG18427 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40603 CGSC7481]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46aa05f0a24e1&page=18675&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table] || || ||
|}


<protect></protect>
===Notes===

==Accessions in Other Databases==
{{BacktoTop}}

{{Gene_accessions_help}}
{| id="X46aa05f14ff9b" class=" tableEdit Gene_accessions_table"
|- {{table_heading_style}}
!|Database!!Accession!!Notes
|-
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11061 ECOCYC:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
|
[http://ecogene.org/geneinfo.php?eg_id=EG11061 EcoGene:EG11061]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/gene?term=948559 GeneID:948559]
|
Escherichia coli str. K-12 substr. MG1655

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.X46aa05f14ff9b&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table] || ||
|}


<protect></protect>
===Notes===

==Links==
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{| id="L46a71f2747b21" class=" tableEdit Links_table"
|- {{table_heading_style}}
!|Name!!URL!!Comments

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18675.L46a71f2747b21&page=18675&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table] || ||
|}


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==References==
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{{RefHelp}}
<references />
__NOTOC__
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:uvr genes]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]