https://ecoliwiki.org/colipedia/api.php?action=feedcontributions&feedformat=atom&user=Charner EcoliWiki - User contributions [en] 2026-11-02T06:09:22Z User contributions MediaWiki 1.43.11 https://ecoliwiki.org/colipedia/index.php?title=pET-43.1_Ek/LIC&diff=316634 pET-43.1 Ek/LIC 2007-10-05T12:56:19Z <p>Charner: New page: ==At a Glance {{HelpIcon|Help:Plasmid}}== &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U47063472ad38d--&gt; &lt;!-- *******************************************************************...</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U47063472ad38d--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |-<br /> !{{table heading style}} |Copy #<br /> |-<br /> !{{table heading style}} |Markers<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |-<br /> !{{table heading style}} |Comments<br /> |-<br /> |colspan='1'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.U47063472ad38d&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U47063472ad38d--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-41_Ek/LIC&diff=316633 pET-41 Ek/LIC 2007-10-05T12:55:34Z <p>Charner: /* References */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-41eklic.JPG|thumb|right|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB317.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning/Exprssion<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-41EkLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> <br /> <br /> &lt;ref name='PMID:16798744'/&gt;<br /> &lt;ref name='PMID:16051668'/&gt;<br /> <br /> <br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt <br /> 181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc <br /> 241 ggatccccga tatccatggt tgaggagaag cccgggctct tgtcgtcgtc atcaccagaa <br /> 301 ccaccaccgg tacccagatc tgggctgtcc atgtgctggc gttcgaattt agcagcagcg <br /> 361 gtttctttca taccaattgc agtactaccg cgtggcacca gacccgcgga gtgatggtga <br /> 421 tggtgatgac cagaaccact agttgaacca tccgattttg gaggatggtc gccaccacca <br /> 481 aacgtggctt gccagccctg caaaggccat gctatatact tgctggattt caagtactta <br /> 541 tcaatttgtg ggatagcttc aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc <br /> 601 aggcacattg ggtccatgta taaaacaaca tcaagagcgt catacaacat gaagtcagga <br /> 661 tgggttacat gatcaccatt taaatatgtt ttatgacata aacgatcttc gaacattttc <br /> 721 agcatttcag gtagcttgct aagaaaatca actttgagag tttcaaagtc tttactatat <br /> 781 gcaattctcg aaacaccgta tctaatatcc aaaaccgctc cttcaagcat tgaaatctct <br /> 841 gcacgctctt ttggacaacc acccaacatg ttgtgcttgt cagctatata acgtatgatg <br /> 901 gccatagact gtgttaattt aacatcacca tcaatataat aaggaagatt gggaaactcc <br /> 961 aaacccaatt caaacttttt gtttcgccat ttatcacctt catcgcgctc atacaaatgc <br /> 1021 tcttcatatt tttcttcaag atattccaaa agaagtcgag tgggttgcac aaggccctta <br /> 1081 attttccaat aacctagtat aggggacata tgtatatctc cttcttaaag ttaaacaaaa <br /> 1141 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt <br /> 1201 tcgcgggatc gagatcgatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg <br /> 1261 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg <br /> 1321 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg <br /> 1381 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca <br /> 1441 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc <br /> 1501 gagatcccgg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg <br /> 1561 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag <br /> 1621 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct <br /> 1681 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc <br /> 1741 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg <br /> 1801 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt <br /> 1861 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg <br /> 1921 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag <br /> 1981 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct <br /> 2041 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg <br /> 2101 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct <br /> 2161 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag <br /> 2221 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa <br /> 2281 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg <br /> 2341 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggacat ctcggtagtg <br /> 2401 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag <br /> 2461 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag <br /> 2521 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg <br /> 2581 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga <br /> 2641 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac <br /> 2701 tcattaggca ccgggatctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt <br /> 2761 ccggtgggcg cggggcatga ctagcatgat cgtgctcctg tcgttgagga cccggctagg <br /> 2821 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag <br /> 2881 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg <br /> 2941 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg <br /> 3001 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg <br /> 3061 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc <br /> 3121 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct <br /> 3181 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc <br /> 3241 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt <br /> 3301 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc <br /> 3361 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg <br /> 3421 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc <br /> 3481 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc <br /> 3541 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag <br /> 3601 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga <br /> 3661 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg <br /> 3721 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat <br /> 3781 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta <br /> 3841 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa <br /> 3901 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc <br /> 3961 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt <br /> 4021 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca <br /> 4081 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg <br /> 4141 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat <br /> 4201 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta <br /> 4261 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct <br /> 4321 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac <br /> 4381 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa <br /> 4441 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa <br /> 4501 actcacgtta agggattttg gtcatgaaca ataaaactgt ctgcttacat aaacagtaat <br /> 4561 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat <br /> 4621 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca <br /> 4681 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat <br /> 4741 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg <br /> 4801 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta <br /> 4861 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca <br /> 4921 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt <br /> 4981 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg <br /> 5041 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa <br /> 5101 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat <br /> 5161 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat <br /> 5221 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc <br /> 5281 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct <br /> 5341 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaaga attaattcat <br /> 5401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt <br /> 5461 tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa <br /> 5521 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa <br /> 5581 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact <br /> 5641 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc <br /> 5701 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa <br /> 5761 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc <br /> 5821 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt <br /> 5881 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca <br /> 5941 ttcgcca <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-41_Ek/LIC&diff=316632 pET-41 Ek/LIC 2007-10-05T12:53:15Z <p>Charner: /* At a Glance {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-41eklic.JPG|thumb|right|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB317.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning/Exprssion<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-41EkLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt <br /> 181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc <br /> 241 ggatccccga tatccatggt tgaggagaag cccgggctct tgtcgtcgtc atcaccagaa <br /> 301 ccaccaccgg tacccagatc tgggctgtcc atgtgctggc gttcgaattt agcagcagcg <br /> 361 gtttctttca taccaattgc agtactaccg cgtggcacca gacccgcgga gtgatggtga <br /> 421 tggtgatgac cagaaccact agttgaacca tccgattttg gaggatggtc gccaccacca <br /> 481 aacgtggctt gccagccctg caaaggccat gctatatact tgctggattt caagtactta <br /> 541 tcaatttgtg ggatagcttc aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc <br /> 601 aggcacattg ggtccatgta taaaacaaca tcaagagcgt catacaacat gaagtcagga <br /> 661 tgggttacat gatcaccatt taaatatgtt ttatgacata aacgatcttc gaacattttc <br /> 721 agcatttcag gtagcttgct aagaaaatca actttgagag tttcaaagtc tttactatat <br /> 781 gcaattctcg aaacaccgta tctaatatcc aaaaccgctc cttcaagcat tgaaatctct <br /> 841 gcacgctctt ttggacaacc acccaacatg ttgtgcttgt cagctatata acgtatgatg <br /> 901 gccatagact gtgttaattt aacatcacca tcaatataat aaggaagatt gggaaactcc <br /> 961 aaacccaatt caaacttttt gtttcgccat ttatcacctt catcgcgctc atacaaatgc <br /> 1021 tcttcatatt tttcttcaag atattccaaa agaagtcgag tgggttgcac aaggccctta <br /> 1081 attttccaat aacctagtat aggggacata tgtatatctc cttcttaaag ttaaacaaaa <br /> 1141 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt <br /> 1201 tcgcgggatc gagatcgatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg <br /> 1261 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg <br /> 1321 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg <br /> 1381 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca <br /> 1441 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc <br /> 1501 gagatcccgg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg <br /> 1561 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag <br /> 1621 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct <br /> 1681 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc <br /> 1741 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg <br /> 1801 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt <br /> 1861 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg <br /> 1921 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag <br /> 1981 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct <br /> 2041 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg <br /> 2101 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct <br /> 2161 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag <br /> 2221 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa <br /> 2281 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg <br /> 2341 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggacat ctcggtagtg <br /> 2401 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag <br /> 2461 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag <br /> 2521 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg <br /> 2581 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga <br /> 2641 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac <br /> 2701 tcattaggca ccgggatctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt <br /> 2761 ccggtgggcg cggggcatga ctagcatgat cgtgctcctg tcgttgagga cccggctagg <br /> 2821 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag <br /> 2881 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg <br /> 2941 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg <br /> 3001 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg <br /> 3061 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc <br /> 3121 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct <br /> 3181 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc <br /> 3241 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt <br /> 3301 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc <br /> 3361 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg <br /> 3421 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc <br /> 3481 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc <br /> 3541 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag <br /> 3601 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga <br /> 3661 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg <br /> 3721 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat <br /> 3781 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta <br /> 3841 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa <br /> 3901 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc <br /> 3961 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt <br /> 4021 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca <br /> 4081 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg <br /> 4141 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat <br /> 4201 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta <br /> 4261 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct <br /> 4321 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac <br /> 4381 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa <br /> 4441 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa <br /> 4501 actcacgtta agggattttg gtcatgaaca ataaaactgt ctgcttacat aaacagtaat <br /> 4561 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat <br /> 4621 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca <br /> 4681 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat <br /> 4741 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg <br /> 4801 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta <br /> 4861 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca <br /> 4921 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt <br /> 4981 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg <br /> 5041 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa <br /> 5101 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat <br /> 5161 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat <br /> 5221 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc <br /> 5281 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct <br /> 5341 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaaga attaattcat <br /> 5401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt <br /> 5461 tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa <br /> 5521 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa <br /> 5581 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact <br /> 5641 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc <br /> 5701 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa <br /> 5761 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc <br /> 5821 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt <br /> 5881 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca <br /> 5941 ttcgcca <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=File:Pet-41eklic.JPG&diff=316631 File:Pet-41eklic.JPG 2007-10-05T12:52:24Z <p>Charner: </p> <hr /> <div></div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-41_Ek/LIC&diff=316630 pET-41 Ek/LIC 2007-10-05T12:50:35Z <p>Charner: /* Sequence {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning/Exprssion<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-41EkLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt <br /> 181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc <br /> 241 ggatccccga tatccatggt tgaggagaag cccgggctct tgtcgtcgtc atcaccagaa <br /> 301 ccaccaccgg tacccagatc tgggctgtcc atgtgctggc gttcgaattt agcagcagcg <br /> 361 gtttctttca taccaattgc agtactaccg cgtggcacca gacccgcgga gtgatggtga <br /> 421 tggtgatgac cagaaccact agttgaacca tccgattttg gaggatggtc gccaccacca <br /> 481 aacgtggctt gccagccctg caaaggccat gctatatact tgctggattt caagtactta <br /> 541 tcaatttgtg ggatagcttc aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc <br /> 601 aggcacattg ggtccatgta taaaacaaca tcaagagcgt catacaacat gaagtcagga <br /> 661 tgggttacat gatcaccatt taaatatgtt ttatgacata aacgatcttc gaacattttc <br /> 721 agcatttcag gtagcttgct aagaaaatca actttgagag tttcaaagtc tttactatat <br /> 781 gcaattctcg aaacaccgta tctaatatcc aaaaccgctc cttcaagcat tgaaatctct <br /> 841 gcacgctctt ttggacaacc acccaacatg ttgtgcttgt cagctatata acgtatgatg <br /> 901 gccatagact gtgttaattt aacatcacca tcaatataat aaggaagatt gggaaactcc <br /> 961 aaacccaatt caaacttttt gtttcgccat ttatcacctt catcgcgctc atacaaatgc <br /> 1021 tcttcatatt tttcttcaag atattccaaa agaagtcgag tgggttgcac aaggccctta <br /> 1081 attttccaat aacctagtat aggggacata tgtatatctc cttcttaaag ttaaacaaaa <br /> 1141 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt <br /> 1201 tcgcgggatc gagatcgatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg <br /> 1261 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg <br /> 1321 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg <br /> 1381 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca <br /> 1441 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc <br /> 1501 gagatcccgg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg <br /> 1561 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag <br /> 1621 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct <br /> 1681 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc <br /> 1741 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg <br /> 1801 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt <br /> 1861 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg <br /> 1921 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag <br /> 1981 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct <br /> 2041 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg <br /> 2101 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct <br /> 2161 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag <br /> 2221 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa <br /> 2281 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg <br /> 2341 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggacat ctcggtagtg <br /> 2401 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag <br /> 2461 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag <br /> 2521 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg <br /> 2581 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga <br /> 2641 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac <br /> 2701 tcattaggca ccgggatctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt <br /> 2761 ccggtgggcg cggggcatga ctagcatgat cgtgctcctg tcgttgagga cccggctagg <br /> 2821 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag <br /> 2881 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg <br /> 2941 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg <br /> 3001 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg <br /> 3061 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc <br /> 3121 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct <br /> 3181 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc <br /> 3241 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt <br /> 3301 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc <br /> 3361 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg <br /> 3421 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc <br /> 3481 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc <br /> 3541 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag <br /> 3601 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga <br /> 3661 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg <br /> 3721 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat <br /> 3781 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta <br /> 3841 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa <br /> 3901 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc <br /> 3961 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt <br /> 4021 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca <br /> 4081 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg <br /> 4141 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat <br /> 4201 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta <br /> 4261 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct <br /> 4321 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac <br /> 4381 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa <br /> 4441 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa <br /> 4501 actcacgtta agggattttg gtcatgaaca ataaaactgt ctgcttacat aaacagtaat <br /> 4561 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat <br /> 4621 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca <br /> 4681 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat <br /> 4741 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg <br /> 4801 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta <br /> 4861 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca <br /> 4921 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt <br /> 4981 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg <br /> 5041 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa <br /> 5101 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat <br /> 5161 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat <br /> 5221 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc <br /> 5281 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct <br /> 5341 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaaga attaattcat <br /> 5401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt <br /> 5461 tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa <br /> 5521 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa <br /> 5581 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact <br /> 5641 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc <br /> 5701 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa <br /> 5761 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc <br /> 5821 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt <br /> 5881 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca <br /> 5941 ttcgcca <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-41_Ek/LIC&diff=316629 pET-41 Ek/LIC 2007-10-05T12:49:04Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning/Exprssion<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-41EkLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-41_Ek/LIC&diff=316628 pET-41 Ek/LIC 2007-10-05T12:47:14Z <p>Charner: New page: ==At a Glance {{HelpIcon|Help:Plasmid}}== &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29--&gt; &lt;!-- *******************************************************************...</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |-<br /> !{{table heading style}} |Copy #<br /> |-<br /> !{{table heading style}} |Markers<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |-<br /> !{{table heading style}} |Comments<br /> |-<br /> |colspan='1'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4706325272f29--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-32_Ek/LIC&diff=316627 pET-32 Ek/LIC 2007-10-05T12:46:06Z <p>Charner: /* References */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-32eklic.JPG|right|thumb|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB161.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning/Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag™ thioredoxin protein, and His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> <br /> &lt;ref name='PMID:15659452'/&gt;<br /> &lt;ref name='PMID:15987703'/&gt;<br /> &lt;ref name='PMID:15585873'/&gt;<br /> <br /> <br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-32_Ek/LIC&diff=316626 pET-32 Ek/LIC 2007-10-05T12:43:19Z <p>Charner: /* At a Glance {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-32eklic.JPG|right|thumb|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB161.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning/Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag™ thioredoxin protein, and His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=File:Pet-32eklic.JPG&diff=316625 File:Pet-32eklic.JPG 2007-10-05T12:42:29Z <p>Charner: </p> <hr /> <div></div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-32_Ek/LIC&diff=316624 pET-32 Ek/LIC 2007-10-05T12:40:45Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning/Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-32 Ek/LIC vector is an Ek/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag™ thioredoxin protein, and His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-32_Ek/LIC&diff=316623 pET-32 Ek/LIC 2007-10-05T12:38:45Z <p>Charner: New page: ==At a Glance {{HelpIcon|Help:Plasmid}}== &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--&gt; &lt;!-- *******************************************************************...</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |-<br /> !{{table heading style}} |Copy #<br /> |-<br /> !{{table heading style}} |Markers<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |-<br /> !{{table heading style}} |Comments<br /> |-<br /> |colspan='1'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.X470630554a72f--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-30_Ek/LIC&diff=316622 pET-30 Ek/LIC 2007-10-05T12:38:05Z <p>Charner: /* History */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-30eklic.JPG|thumb|right|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB162.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning/Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69077-000.HTM Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-30 Ek/LIC vector is an Ek/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History==<br /> [http://www.emdbiosciences.com/product/69077 Novagen]<br /> <br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions. The LIC method takes advantage of the 3’ →5’ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single-stranded overhangs in the vector and the insert, so that the vast majority of annealed products consist of the desired molecules. The annealed LIC vector and insert are transformed into competent E. coli cells, and covalent bonds are formed at the vector-insert junctions within the cell to yield circular plasmid. Directional cloning of the insert is achieved with minimal non-recombinant background, and cloning is so efficient that virtually all of the resulting colonies contain the desired recombinant. <br /> <br /> [http://www.emdbiosciences.com/product/69077 Novagen]<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> <br /> &lt;ref name='PMID:17452359'/&gt;<br /> &lt;ref name='PMID:15557323'/&gt;<br /> &lt;ref name='PMID:16183635'/&gt;<br /> &lt;ref name='PMID:15471861'/&gt;<br /> &lt;ref name='PMID:14500468'/&gt;<br /> <br /> <br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA <br /> 51 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA <br /> 101 CTCAGCTTCC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT <br /> 151 GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT CGACGGAGCT CGAATTCGGA <br /> 201 TCCGATATCG CCATGGTTGA GGAGAAGCCC GGGCTCTTGT CGTCGTCATC <br /> 251 GGTACCCAGA TCTGGGCTGT CCATGTGCTG GCGTTCGAAT TTAGCAGCAG <br /> 301 CGGTTTCTTT CATACCAGAA CCGCGTGGCA CCAGACCAGA AGAATGATGA <br /> 351 TGATGATGGT GCATATGTAT ATCTCCTTCT TAAAGTTAAA CAAAATTATT <br /> 401 TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG TGAGTCGTAT <br /> 451 TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG <br /> 501 TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC <br /> 551 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC <br /> 601 TTGTTTCGGC GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG <br /> 651 GCGCCATCTC CTTGCATGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC <br /> 701 CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA <br /> 751 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG <br /> 801 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC <br /> 851 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG <br /> 901 TTTCCCGCGT GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA <br /> 951 AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA ACCGCGTGGC <br /> 1001 ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA <br /> 1051 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC <br /> 1101 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG <br /> 1151 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA <br /> 1201 GTGGGCTGAT CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG <br /> 1251 GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TCTCTGACCA <br /> 1301 GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG <br /> 1351 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG <br /> 1401 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA <br /> 1451 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT <br /> 1501 GGAGTGCCAT GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC <br /> 1551 ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG CGCTGGGCGC <br /> 1601 AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GACATCTCGG <br /> 1651 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA <br /> 1701 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG <br /> 1751 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC <br /> 1801 CCGTCTCACT GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC <br /> 1851 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT <br /> 1901 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG <br /> 1951 CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA <br /> 2001 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC <br /> 2051 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG <br /> 2101 CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT <br /> 2151 CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT <br /> 2201 TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC <br /> 2251 GCCGGCATGG CGGCCCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG <br /> 2301 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC <br /> 2351 CGATACGCGA GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC <br /> 2401 TGAGCAACAA CATGAATGGT CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA <br /> 2451 AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC TGCATCGCAG <br /> 2501 GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC <br /> 2551 TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC <br /> 2601 CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG <br /> 2651 TAACCCGTAT CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC <br /> 2701 ATGAACAGAA ATCCCCCTTA CACGGAGGCA TCAGTGACCA AACAGGAAAA <br /> 2751 AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA TTAACGCTTC <br /> 2801 TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA <br /> 2851 TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT <br /> 2901 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA <br /> 2951 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG <br /> 3001 TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG <br /> 3051 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT <br /> 3101 ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG <br /> 3151 GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC <br /> 3201 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG <br /> 3251 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG <br /> 3301 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG <br /> 3351 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC <br /> 3401 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT <br /> 3451 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA <br /> 3501 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT <br /> 3551 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT <br /> 3601 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG <br /> 3651 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG <br /> 3701 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC <br /> 3751 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG <br /> 3801 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT <br /> 3851 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT <br /> 3901 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC <br /> 3951 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT <br /> 4001 TAAGGGATTT TGGTCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA <br /> 4051 ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC TTGCTCTAGG <br /> 4101 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC <br /> 4151 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA <br /> 4201 AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC <br /> 4251 AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT <br /> 4301 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT <br /> 4351 TACTCACCAC TGCGATCCCC GGGAAAACAG CATTCCAGGT ATTAGAAGAA <br /> 4401 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG <br /> 4451 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG <br /> 4501 TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT <br /> 4551 GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG <br /> 4601 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC <br /> 4651 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA <br /> 4701 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT <br /> 4751 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC <br /> 4801 GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG <br /> 4851 TTTCATTTGA TGCTCGATGA GTTTTTCTAA GAATTAATTC ATGAGCGGAT <br /> 4901 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA <br /> 4951 TTTCCCCGAA AAGTGCCACC TGAAATTGTA AACGTTAATA TTTTGTTAAA <br /> 5001 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG <br /> 5051 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG <br /> 5101 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC <br /> 5151 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG <br /> 5201 AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA <br /> 5251 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC <br /> 5301 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA <br /> 5351 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC <br /> 5401 GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCA<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-30_Ek/LIC&diff=316621 pET-30 Ek/LIC 2007-10-05T12:37:32Z <p>Charner: /* History */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-30eklic.JPG|thumb|right|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB162.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning/Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69077-000.HTM Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-30 Ek/LIC vector is an Ek/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History==<br /> [http://www.emdbiosciences.com/product/69077 Novagen]<br /> <br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions (Aslanidis 1990, Haun 1992). The LIC method takes advantage of the 3’ →5’ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single-stranded overhangs in the vector and the insert, so that the vast majority of annealed products consist of the desired molecules. The annealed LIC vector and insert are transformed into competent E. coli cells, and covalent bonds are formed at the vector-insert junctions within the cell to yield circular plasmid. Directional cloning of the insert is achieved with minimal non-recombinant background, and cloning is so efficient that virtually all of the resulting colonies contain the desired recombinant. <br /> <br /> [http://www.emdbiosciences.com/product/69077 Novagen]<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> <br /> &lt;ref name='PMID:17452359'/&gt;<br /> &lt;ref name='PMID:15557323'/&gt;<br /> &lt;ref name='PMID:16183635'/&gt;<br /> &lt;ref name='PMID:15471861'/&gt;<br /> &lt;ref name='PMID:14500468'/&gt;<br /> <br /> <br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA <br /> 51 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA <br /> 101 CTCAGCTTCC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT <br /> 151 GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT CGACGGAGCT CGAATTCGGA <br /> 201 TCCGATATCG CCATGGTTGA GGAGAAGCCC GGGCTCTTGT CGTCGTCATC <br /> 251 GGTACCCAGA TCTGGGCTGT CCATGTGCTG GCGTTCGAAT TTAGCAGCAG <br /> 301 CGGTTTCTTT CATACCAGAA CCGCGTGGCA CCAGACCAGA AGAATGATGA <br /> 351 TGATGATGGT GCATATGTAT ATCTCCTTCT TAAAGTTAAA CAAAATTATT <br /> 401 TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG TGAGTCGTAT <br /> 451 TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG <br /> 501 TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC <br /> 551 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC <br /> 601 TTGTTTCGGC GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG <br /> 651 GCGCCATCTC CTTGCATGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC <br /> 701 CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA <br /> 751 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG <br /> 801 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC <br /> 851 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG <br /> 901 TTTCCCGCGT GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA <br /> 951 AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA ACCGCGTGGC <br /> 1001 ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA <br /> 1051 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC <br /> 1101 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG <br /> 1151 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA <br /> 1201 GTGGGCTGAT CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG <br /> 1251 GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TCTCTGACCA <br /> 1301 GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG <br /> 1351 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG <br /> 1401 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA <br /> 1451 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT <br /> 1501 GGAGTGCCAT GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC <br /> 1551 ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG CGCTGGGCGC <br /> 1601 AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GACATCTCGG <br /> 1651 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA <br /> 1701 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG <br /> 1751 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC <br /> 1801 CCGTCTCACT GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC <br /> 1851 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT <br /> 1901 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG <br /> 1951 CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA <br /> 2001 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC <br /> 2051 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG <br /> 2101 CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT <br /> 2151 CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT <br /> 2201 TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC <br /> 2251 GCCGGCATGG CGGCCCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG <br /> 2301 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC <br /> 2351 CGATACGCGA GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC <br /> 2401 TGAGCAACAA CATGAATGGT CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA <br /> 2451 AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC TGCATCGCAG <br /> 2501 GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC <br /> 2551 TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC <br /> 2601 CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG <br /> 2651 TAACCCGTAT CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC <br /> 2701 ATGAACAGAA ATCCCCCTTA CACGGAGGCA TCAGTGACCA AACAGGAAAA <br /> 2751 AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA TTAACGCTTC <br /> 2801 TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA <br /> 2851 TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT <br /> 2901 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA <br /> 2951 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG <br /> 3001 TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG <br /> 3051 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT <br /> 3101 ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG <br /> 3151 GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC <br /> 3201 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG <br /> 3251 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG <br /> 3301 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG <br /> 3351 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC <br /> 3401 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT <br /> 3451 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA <br /> 3501 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT <br /> 3551 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT <br /> 3601 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG <br /> 3651 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG <br /> 3701 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC <br /> 3751 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG <br /> 3801 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT <br /> 3851 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT <br /> 3901 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC <br /> 3951 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT <br /> 4001 TAAGGGATTT TGGTCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA <br /> 4051 ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC TTGCTCTAGG <br /> 4101 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC <br /> 4151 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA <br /> 4201 AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC <br /> 4251 AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT <br /> 4301 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT <br /> 4351 TACTCACCAC TGCGATCCCC GGGAAAACAG CATTCCAGGT ATTAGAAGAA <br /> 4401 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG <br /> 4451 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG <br /> 4501 TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT <br /> 4551 GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG <br /> 4601 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC <br /> 4651 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA <br /> 4701 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT <br /> 4751 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC <br /> 4801 GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG <br /> 4851 TTTCATTTGA TGCTCGATGA GTTTTTCTAA GAATTAATTC ATGAGCGGAT <br /> 4901 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA <br /> 4951 TTTCCCCGAA AAGTGCCACC TGAAATTGTA AACGTTAATA TTTTGTTAAA <br /> 5001 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG <br /> 5051 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG <br /> 5101 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC <br /> 5151 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG <br /> 5201 AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA <br /> 5251 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC <br /> 5301 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA <br /> 5351 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC <br /> 5401 GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCA<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-30_Ek/LIC&diff=316620 pET-30 Ek/LIC 2007-10-05T12:37:14Z <p>Charner: /* History */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-30eklic.JPG|thumb|right|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB162.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning/Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69077-000.HTM Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-30 Ek/LIC vector is an Ek/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History==<br /> [http://www.emdbiosciences.com/product/69077 Novagen]<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions (Aslanidis 1990, Haun 1992). The LIC method takes advantage of the 3’ →5’ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single-stranded overhangs in the vector and the insert, so that the vast majority of annealed products consist of the desired molecules. The annealed LIC vector and insert are transformed into competent E. coli cells, and covalent bonds are formed at the vector-insert junctions within the cell to yield circular plasmid. Directional cloning of the insert is achieved with minimal non-recombinant background, and cloning is so efficient that virtually all of the resulting colonies contain the desired recombinant. <br /> [http://www.emdbiosciences.com/product/69077 Novagen]<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> <br /> &lt;ref name='PMID:17452359'/&gt;<br /> &lt;ref name='PMID:15557323'/&gt;<br /> &lt;ref name='PMID:16183635'/&gt;<br /> &lt;ref name='PMID:15471861'/&gt;<br /> &lt;ref name='PMID:14500468'/&gt;<br /> <br /> <br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA <br /> 51 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA <br /> 101 CTCAGCTTCC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT <br /> 151 GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT CGACGGAGCT CGAATTCGGA <br /> 201 TCCGATATCG CCATGGTTGA GGAGAAGCCC GGGCTCTTGT CGTCGTCATC <br /> 251 GGTACCCAGA TCTGGGCTGT CCATGTGCTG GCGTTCGAAT TTAGCAGCAG <br /> 301 CGGTTTCTTT CATACCAGAA CCGCGTGGCA CCAGACCAGA AGAATGATGA <br /> 351 TGATGATGGT GCATATGTAT ATCTCCTTCT TAAAGTTAAA CAAAATTATT <br /> 401 TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG TGAGTCGTAT <br /> 451 TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG <br /> 501 TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC <br /> 551 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC <br /> 601 TTGTTTCGGC GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG <br /> 651 GCGCCATCTC CTTGCATGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC <br /> 701 CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA <br /> 751 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG <br /> 801 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC <br /> 851 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG <br /> 901 TTTCCCGCGT GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA <br /> 951 AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA ACCGCGTGGC <br /> 1001 ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA <br /> 1051 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC <br /> 1101 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG <br /> 1151 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA <br /> 1201 GTGGGCTGAT CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG <br /> 1251 GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TCTCTGACCA <br /> 1301 GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG <br /> 1351 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG <br /> 1401 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA <br /> 1451 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT <br /> 1501 GGAGTGCCAT GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC <br /> 1551 ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG CGCTGGGCGC <br /> 1601 AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GACATCTCGG <br /> 1651 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA <br /> 1701 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG <br /> 1751 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC <br /> 1801 CCGTCTCACT GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC <br /> 1851 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT <br /> 1901 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG <br /> 1951 CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA <br /> 2001 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC <br /> 2051 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG <br /> 2101 CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT <br /> 2151 CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT <br /> 2201 TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC <br /> 2251 GCCGGCATGG CGGCCCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG <br /> 2301 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC <br /> 2351 CGATACGCGA GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC <br /> 2401 TGAGCAACAA CATGAATGGT CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA <br /> 2451 AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC TGCATCGCAG <br /> 2501 GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC <br /> 2551 TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC <br /> 2601 CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG <br /> 2651 TAACCCGTAT CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC <br /> 2701 ATGAACAGAA ATCCCCCTTA CACGGAGGCA TCAGTGACCA AACAGGAAAA <br /> 2751 AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA TTAACGCTTC <br /> 2801 TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA <br /> 2851 TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT <br /> 2901 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA <br /> 2951 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG <br /> 3001 TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG <br /> 3051 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT <br /> 3101 ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG <br /> 3151 GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC <br /> 3201 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG <br /> 3251 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG <br /> 3301 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG <br /> 3351 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC <br /> 3401 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT <br /> 3451 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA <br /> 3501 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT <br /> 3551 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT <br /> 3601 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG <br /> 3651 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG <br /> 3701 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC <br /> 3751 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG <br /> 3801 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT <br /> 3851 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT <br /> 3901 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC <br /> 3951 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT <br /> 4001 TAAGGGATTT TGGTCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA <br /> 4051 ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC TTGCTCTAGG <br /> 4101 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC <br /> 4151 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA <br /> 4201 AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC <br /> 4251 AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT <br /> 4301 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT <br /> 4351 TACTCACCAC TGCGATCCCC GGGAAAACAG CATTCCAGGT ATTAGAAGAA <br /> 4401 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG <br /> 4451 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG <br /> 4501 TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT <br /> 4551 GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG <br /> 4601 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC <br /> 4651 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA <br /> 4701 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT <br /> 4751 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC <br /> 4801 GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG <br /> 4851 TTTCATTTGA TGCTCGATGA GTTTTTCTAA GAATTAATTC ATGAGCGGAT <br /> 4901 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA <br /> 4951 TTTCCCCGAA AAGTGCCACC TGAAATTGTA AACGTTAATA TTTTGTTAAA <br /> 5001 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG <br /> 5051 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG <br /> 5101 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC <br /> 5151 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG <br /> 5201 AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA <br /> 5251 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC <br /> 5301 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA <br /> 5351 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC <br /> 5401 GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCA<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-30_Ek/LIC&diff=316619 pET-30 Ek/LIC 2007-10-05T12:36:52Z <p>Charner: /* History {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-30eklic.JPG|thumb|right|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB162.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning/Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69077-000.HTM Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-30 Ek/LIC vector is an Ek/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History==<br /> [http://www.emdbiosciences.com/product/69077Novagen]<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions (Aslanidis 1990, Haun 1992). The LIC method takes advantage of the 3’ →5’ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single-stranded overhangs in the vector and the insert, so that the vast majority of annealed products consist of the desired molecules. The annealed LIC vector and insert are transformed into competent E. coli cells, and covalent bonds are formed at the vector-insert junctions within the cell to yield circular plasmid. Directional cloning of the insert is achieved with minimal non-recombinant background, and cloning is so efficient that virtually all of the resulting colonies contain the desired recombinant. <br /> [http://www.emdbiosciences.com/product/69077Novagen]<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> <br /> &lt;ref name='PMID:17452359'/&gt;<br /> &lt;ref name='PMID:15557323'/&gt;<br /> &lt;ref name='PMID:16183635'/&gt;<br /> &lt;ref name='PMID:15471861'/&gt;<br /> &lt;ref name='PMID:14500468'/&gt;<br /> <br /> <br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA <br /> 51 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA <br /> 101 CTCAGCTTCC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT <br /> 151 GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT CGACGGAGCT CGAATTCGGA <br /> 201 TCCGATATCG CCATGGTTGA GGAGAAGCCC GGGCTCTTGT CGTCGTCATC <br /> 251 GGTACCCAGA TCTGGGCTGT CCATGTGCTG GCGTTCGAAT TTAGCAGCAG <br /> 301 CGGTTTCTTT CATACCAGAA CCGCGTGGCA CCAGACCAGA AGAATGATGA <br /> 351 TGATGATGGT GCATATGTAT ATCTCCTTCT TAAAGTTAAA CAAAATTATT <br /> 401 TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG TGAGTCGTAT <br /> 451 TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG <br /> 501 TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC <br /> 551 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC <br /> 601 TTGTTTCGGC GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG <br /> 651 GCGCCATCTC CTTGCATGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC <br /> 701 CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA <br /> 751 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG <br /> 801 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC <br /> 851 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG <br /> 901 TTTCCCGCGT GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA <br /> 951 AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA ACCGCGTGGC <br /> 1001 ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA <br /> 1051 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC <br /> 1101 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG <br /> 1151 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA <br /> 1201 GTGGGCTGAT CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG <br /> 1251 GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TCTCTGACCA <br /> 1301 GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG <br /> 1351 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG <br /> 1401 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA <br /> 1451 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT <br /> 1501 GGAGTGCCAT GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC <br /> 1551 ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG CGCTGGGCGC <br /> 1601 AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GACATCTCGG <br /> 1651 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA <br /> 1701 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG <br /> 1751 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC <br /> 1801 CCGTCTCACT GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC <br /> 1851 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT <br /> 1901 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG <br /> 1951 CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA <br /> 2001 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC <br /> 2051 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG <br /> 2101 CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT <br /> 2151 CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT <br /> 2201 TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC <br /> 2251 GCCGGCATGG CGGCCCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG <br /> 2301 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC <br /> 2351 CGATACGCGA GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC <br /> 2401 TGAGCAACAA CATGAATGGT CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA <br /> 2451 AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC TGCATCGCAG <br /> 2501 GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC <br /> 2551 TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC <br /> 2601 CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG <br /> 2651 TAACCCGTAT CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC <br /> 2701 ATGAACAGAA ATCCCCCTTA CACGGAGGCA TCAGTGACCA AACAGGAAAA <br /> 2751 AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA TTAACGCTTC <br /> 2801 TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA <br /> 2851 TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT <br /> 2901 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA <br /> 2951 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG <br /> 3001 TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG <br /> 3051 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT <br /> 3101 ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG <br /> 3151 GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC <br /> 3201 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG <br /> 3251 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG <br /> 3301 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG <br /> 3351 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC <br /> 3401 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT <br /> 3451 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA <br /> 3501 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT <br /> 3551 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT <br /> 3601 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG <br /> 3651 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG <br /> 3701 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC <br /> 3751 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG <br /> 3801 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT <br /> 3851 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT <br /> 3901 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC <br /> 3951 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT <br /> 4001 TAAGGGATTT TGGTCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA <br /> 4051 ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC TTGCTCTAGG <br /> 4101 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC <br /> 4151 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA <br /> 4201 AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC <br /> 4251 AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT <br /> 4301 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT <br /> 4351 TACTCACCAC TGCGATCCCC GGGAAAACAG CATTCCAGGT ATTAGAAGAA <br /> 4401 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG <br /> 4451 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG <br /> 4501 TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT <br /> 4551 GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG <br /> 4601 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC <br /> 4651 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA <br /> 4701 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT <br /> 4751 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC <br /> 4801 GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG <br /> 4851 TTTCATTTGA TGCTCGATGA GTTTTTCTAA GAATTAATTC ATGAGCGGAT <br /> 4901 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA <br /> 4951 TTTCCCCGAA AAGTGCCACC TGAAATTGTA AACGTTAATA TTTTGTTAAA <br /> 5001 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG <br /> 5051 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG <br /> 5101 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC <br /> 5151 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG <br /> 5201 AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA <br /> 5251 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC <br /> 5301 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA <br /> 5351 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC <br /> 5401 GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCA<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-30_Ek/LIC&diff=316618 pET-30 Ek/LIC 2007-10-05T12:35:25Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-30eklic.JPG|thumb|right|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB162.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning/Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69077-000.HTM Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-30 Ek/LIC vector is an Ek/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> <br /> &lt;ref name='PMID:17452359'/&gt;<br /> &lt;ref name='PMID:15557323'/&gt;<br /> &lt;ref name='PMID:16183635'/&gt;<br /> &lt;ref name='PMID:15471861'/&gt;<br /> &lt;ref name='PMID:14500468'/&gt;<br /> <br /> <br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA <br /> 51 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA <br /> 101 CTCAGCTTCC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT <br /> 151 GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT CGACGGAGCT CGAATTCGGA <br /> 201 TCCGATATCG CCATGGTTGA GGAGAAGCCC GGGCTCTTGT CGTCGTCATC <br /> 251 GGTACCCAGA TCTGGGCTGT CCATGTGCTG GCGTTCGAAT TTAGCAGCAG <br /> 301 CGGTTTCTTT CATACCAGAA CCGCGTGGCA CCAGACCAGA AGAATGATGA <br /> 351 TGATGATGGT GCATATGTAT ATCTCCTTCT TAAAGTTAAA CAAAATTATT <br /> 401 TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG TGAGTCGTAT <br /> 451 TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG <br /> 501 TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC <br /> 551 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC <br /> 601 TTGTTTCGGC GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG <br /> 651 GCGCCATCTC CTTGCATGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC <br /> 701 CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA <br /> 751 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG <br /> 801 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC <br /> 851 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG <br /> 901 TTTCCCGCGT GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA <br /> 951 AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA ACCGCGTGGC <br /> 1001 ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA <br /> 1051 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC <br /> 1101 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG <br /> 1151 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA <br /> 1201 GTGGGCTGAT CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG <br /> 1251 GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TCTCTGACCA <br /> 1301 GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG <br /> 1351 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG <br /> 1401 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA <br /> 1451 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT <br /> 1501 GGAGTGCCAT GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC <br /> 1551 ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG CGCTGGGCGC <br /> 1601 AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GACATCTCGG <br /> 1651 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA <br /> 1701 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG <br /> 1751 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC <br /> 1801 CCGTCTCACT GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC <br /> 1851 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT <br /> 1901 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG <br /> 1951 CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA <br /> 2001 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC <br /> 2051 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG <br /> 2101 CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT <br /> 2151 CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT <br /> 2201 TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC <br /> 2251 GCCGGCATGG CGGCCCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG <br /> 2301 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC <br /> 2351 CGATACGCGA GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC <br /> 2401 TGAGCAACAA CATGAATGGT CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA <br /> 2451 AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC TGCATCGCAG <br /> 2501 GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC <br /> 2551 TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC <br /> 2601 CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG <br /> 2651 TAACCCGTAT CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC <br /> 2701 ATGAACAGAA ATCCCCCTTA CACGGAGGCA TCAGTGACCA AACAGGAAAA <br /> 2751 AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA TTAACGCTTC <br /> 2801 TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA <br /> 2851 TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT <br /> 2901 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA <br /> 2951 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG <br /> 3001 TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG <br /> 3051 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT <br /> 3101 ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG <br /> 3151 GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC <br /> 3201 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG <br /> 3251 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG <br /> 3301 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG <br /> 3351 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC <br /> 3401 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT <br /> 3451 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA <br /> 3501 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT <br /> 3551 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT <br /> 3601 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG <br /> 3651 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG <br /> 3701 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC <br /> 3751 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG <br /> 3801 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT <br /> 3851 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT <br /> 3901 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC <br /> 3951 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT <br /> 4001 TAAGGGATTT TGGTCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA <br /> 4051 ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC TTGCTCTAGG <br /> 4101 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC <br /> 4151 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA <br /> 4201 AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC <br /> 4251 AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT <br /> 4301 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT <br /> 4351 TACTCACCAC TGCGATCCCC GGGAAAACAG CATTCCAGGT ATTAGAAGAA <br /> 4401 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG <br /> 4451 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG <br /> 4501 TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT <br /> 4551 GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG <br /> 4601 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC <br /> 4651 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA <br /> 4701 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT <br /> 4751 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC <br /> 4801 GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG <br /> 4851 TTTCATTTGA TGCTCGATGA GTTTTTCTAA GAATTAATTC ATGAGCGGAT <br /> 4901 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA <br /> 4951 TTTCCCCGAA AAGTGCCACC TGAAATTGTA AACGTTAATA TTTTGTTAAA <br /> 5001 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG <br /> 5051 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG <br /> 5101 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC <br /> 5151 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG <br /> 5201 AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA <br /> 5251 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC <br /> 5301 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA <br /> 5351 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC <br /> 5401 GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCA<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-30_Ek/LIC&diff=316617 pET-30 Ek/LIC 2007-10-05T12:33:25Z <p>Charner: /* References */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-30eklic.JPG|thumb|right|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB162.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69077-000.HTM Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-30 Ek/LIC vector is an Ek/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> <br /> &lt;ref name='PMID:17452359'/&gt;<br /> &lt;ref name='PMID:15557323'/&gt;<br /> &lt;ref name='PMID:16183635'/&gt;<br /> &lt;ref name='PMID:15471861'/&gt;<br /> &lt;ref name='PMID:14500468'/&gt;<br /> <br /> <br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA <br /> 51 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA <br /> 101 CTCAGCTTCC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT <br /> 151 GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT CGACGGAGCT CGAATTCGGA <br /> 201 TCCGATATCG CCATGGTTGA GGAGAAGCCC GGGCTCTTGT CGTCGTCATC <br /> 251 GGTACCCAGA TCTGGGCTGT CCATGTGCTG GCGTTCGAAT TTAGCAGCAG <br /> 301 CGGTTTCTTT CATACCAGAA CCGCGTGGCA CCAGACCAGA AGAATGATGA <br /> 351 TGATGATGGT GCATATGTAT ATCTCCTTCT TAAAGTTAAA CAAAATTATT <br /> 401 TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG TGAGTCGTAT <br /> 451 TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG <br /> 501 TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC <br /> 551 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC <br /> 601 TTGTTTCGGC GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG <br /> 651 GCGCCATCTC CTTGCATGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC <br /> 701 CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA <br /> 751 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG <br /> 801 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC <br /> 851 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG <br /> 901 TTTCCCGCGT GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA <br /> 951 AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA ACCGCGTGGC <br /> 1001 ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA <br /> 1051 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC <br /> 1101 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG <br /> 1151 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA <br /> 1201 GTGGGCTGAT CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG <br /> 1251 GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TCTCTGACCA <br /> 1301 GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG <br /> 1351 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG <br /> 1401 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA <br /> 1451 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT <br /> 1501 GGAGTGCCAT GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC <br /> 1551 ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG CGCTGGGCGC <br /> 1601 AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GACATCTCGG <br /> 1651 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA <br /> 1701 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG <br /> 1751 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC <br /> 1801 CCGTCTCACT GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC <br /> 1851 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT <br /> 1901 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG <br /> 1951 CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA <br /> 2001 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC <br /> 2051 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG <br /> 2101 CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT <br /> 2151 CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT <br /> 2201 TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC <br /> 2251 GCCGGCATGG CGGCCCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG <br /> 2301 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC <br /> 2351 CGATACGCGA GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC <br /> 2401 TGAGCAACAA CATGAATGGT CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA <br /> 2451 AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC TGCATCGCAG <br /> 2501 GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC <br /> 2551 TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC <br /> 2601 CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG <br /> 2651 TAACCCGTAT CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC <br /> 2701 ATGAACAGAA ATCCCCCTTA CACGGAGGCA TCAGTGACCA AACAGGAAAA <br /> 2751 AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA TTAACGCTTC <br /> 2801 TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA <br /> 2851 TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT <br /> 2901 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA <br /> 2951 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG <br /> 3001 TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG <br /> 3051 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT <br /> 3101 ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG <br /> 3151 GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC <br /> 3201 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG <br /> 3251 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG <br /> 3301 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG <br /> 3351 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC <br /> 3401 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT <br /> 3451 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA <br /> 3501 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT <br /> 3551 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT <br /> 3601 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG <br /> 3651 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG <br /> 3701 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC <br /> 3751 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG <br /> 3801 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT <br /> 3851 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT <br /> 3901 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC <br /> 3951 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT <br /> 4001 TAAGGGATTT TGGTCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA <br /> 4051 ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC TTGCTCTAGG <br /> 4101 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC <br /> 4151 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA <br /> 4201 AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC <br /> 4251 AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT <br /> 4301 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT <br /> 4351 TACTCACCAC TGCGATCCCC GGGAAAACAG CATTCCAGGT ATTAGAAGAA <br /> 4401 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG <br /> 4451 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG <br /> 4501 TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT <br /> 4551 GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG <br /> 4601 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC <br /> 4651 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA <br /> 4701 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT <br /> 4751 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC <br /> 4801 GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG <br /> 4851 TTTCATTTGA TGCTCGATGA GTTTTTCTAA GAATTAATTC ATGAGCGGAT <br /> 4901 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA <br /> 4951 TTTCCCCGAA AAGTGCCACC TGAAATTGTA AACGTTAATA TTTTGTTAAA <br /> 5001 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG <br /> 5051 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG <br /> 5101 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC <br /> 5151 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG <br /> 5201 AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA <br /> 5251 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC <br /> 5301 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA <br /> 5351 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC <br /> 5401 GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCA<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-30_Ek/LIC&diff=316616 pET-30 Ek/LIC 2007-10-05T12:27:45Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-30eklic.JPG|thumb|right|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB162.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69077-000.HTM Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-30 Ek/LIC vector is an Ek/LIC version of pET-30b(+). It is designed for cloning and high-level expression of target proteins fused with the His•Tag® and S®Tag™ coding sequences that are cleavable with enterokinase (Ek) protease.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA <br /> 51 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA <br /> 101 CTCAGCTTCC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT <br /> 151 GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT CGACGGAGCT CGAATTCGGA <br /> 201 TCCGATATCG CCATGGTTGA GGAGAAGCCC GGGCTCTTGT CGTCGTCATC <br /> 251 GGTACCCAGA TCTGGGCTGT CCATGTGCTG GCGTTCGAAT TTAGCAGCAG <br /> 301 CGGTTTCTTT CATACCAGAA CCGCGTGGCA CCAGACCAGA AGAATGATGA <br /> 351 TGATGATGGT GCATATGTAT ATCTCCTTCT TAAAGTTAAA CAAAATTATT <br /> 401 TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG TGAGTCGTAT <br /> 451 TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG <br /> 501 TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC <br /> 551 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC <br /> 601 TTGTTTCGGC GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG <br /> 651 GCGCCATCTC CTTGCATGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC <br /> 701 CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA <br /> 751 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG <br /> 801 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC <br /> 851 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG <br /> 901 TTTCCCGCGT GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA <br /> 951 AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA ACCGCGTGGC <br /> 1001 ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA <br /> 1051 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC <br /> 1101 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG <br /> 1151 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA <br /> 1201 GTGGGCTGAT CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG <br /> 1251 GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TCTCTGACCA <br /> 1301 GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG <br /> 1351 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG <br /> 1401 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA <br /> 1451 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT <br /> 1501 GGAGTGCCAT GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC <br /> 1551 ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG CGCTGGGCGC <br /> 1601 AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GACATCTCGG <br /> 1651 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA <br /> 1701 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG <br /> 1751 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC <br /> 1801 CCGTCTCACT GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC <br /> 1851 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT <br /> 1901 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG <br /> 1951 CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA <br /> 2001 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC <br /> 2051 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG <br /> 2101 CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT <br /> 2151 CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT <br /> 2201 TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC <br /> 2251 GCCGGCATGG CGGCCCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG <br /> 2301 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC <br /> 2351 CGATACGCGA GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC <br /> 2401 TGAGCAACAA CATGAATGGT CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA <br /> 2451 AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC TGCATCGCAG <br /> 2501 GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC <br /> 2551 TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC <br /> 2601 CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG <br /> 2651 TAACCCGTAT CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC <br /> 2701 ATGAACAGAA ATCCCCCTTA CACGGAGGCA TCAGTGACCA AACAGGAAAA <br /> 2751 AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA TTAACGCTTC <br /> 2801 TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA <br /> 2851 TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT <br /> 2901 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA <br /> 2951 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG <br /> 3001 TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG <br /> 3051 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT <br /> 3101 ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG <br /> 3151 GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC <br /> 3201 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG <br /> 3251 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG <br /> 3301 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG <br /> 3351 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC <br /> 3401 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT <br /> 3451 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA <br /> 3501 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT <br /> 3551 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT <br /> 3601 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG <br /> 3651 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG <br /> 3701 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC <br /> 3751 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG <br /> 3801 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT <br /> 3851 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT <br /> 3901 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC <br /> 3951 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT <br /> 4001 TAAGGGATTT TGGTCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA <br /> 4051 ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC TTGCTCTAGG <br /> 4101 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC <br /> 4151 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA <br /> 4201 AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC <br /> 4251 AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT <br /> 4301 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT <br /> 4351 TACTCACCAC TGCGATCCCC GGGAAAACAG CATTCCAGGT ATTAGAAGAA <br /> 4401 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG <br /> 4451 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG <br /> 4501 TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT <br /> 4551 GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG <br /> 4601 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC <br /> 4651 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA <br /> 4701 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT <br /> 4751 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC <br /> 4801 GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG <br /> 4851 TTTCATTTGA TGCTCGATGA GTTTTTCTAA GAATTAATTC ATGAGCGGAT <br /> 4901 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA <br /> 4951 TTTCCCCGAA AAGTGCCACC TGAAATTGTA AACGTTAATA TTTTGTTAAA <br /> 5001 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG <br /> 5051 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG <br /> 5101 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC <br /> 5151 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG <br /> 5201 AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA <br /> 5251 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC <br /> 5301 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA <br /> 5351 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC <br /> 5401 GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCA<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-30_Ek/LIC&diff=316615 pET-30 Ek/LIC 2007-10-05T12:26:00Z <p>Charner: /* At a Glance {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-30eklic.JPG|thumb|right|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB162.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69077-000.HTM Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA <br /> 51 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA <br /> 101 CTCAGCTTCC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT <br /> 151 GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT CGACGGAGCT CGAATTCGGA <br /> 201 TCCGATATCG CCATGGTTGA GGAGAAGCCC GGGCTCTTGT CGTCGTCATC <br /> 251 GGTACCCAGA TCTGGGCTGT CCATGTGCTG GCGTTCGAAT TTAGCAGCAG <br /> 301 CGGTTTCTTT CATACCAGAA CCGCGTGGCA CCAGACCAGA AGAATGATGA <br /> 351 TGATGATGGT GCATATGTAT ATCTCCTTCT TAAAGTTAAA CAAAATTATT <br /> 401 TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG TGAGTCGTAT <br /> 451 TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG <br /> 501 TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC <br /> 551 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC <br /> 601 TTGTTTCGGC GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG <br /> 651 GCGCCATCTC CTTGCATGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC <br /> 701 CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA <br /> 751 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG <br /> 801 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC <br /> 851 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG <br /> 901 TTTCCCGCGT GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA <br /> 951 AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA ACCGCGTGGC <br /> 1001 ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA <br /> 1051 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC <br /> 1101 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG <br /> 1151 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA <br /> 1201 GTGGGCTGAT CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG <br /> 1251 GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TCTCTGACCA <br /> 1301 GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG <br /> 1351 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG <br /> 1401 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA <br /> 1451 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT <br /> 1501 GGAGTGCCAT GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC <br /> 1551 ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG CGCTGGGCGC <br /> 1601 AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GACATCTCGG <br /> 1651 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA <br /> 1701 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG <br /> 1751 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC <br /> 1801 CCGTCTCACT GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC <br /> 1851 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT <br /> 1901 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG <br /> 1951 CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA <br /> 2001 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC <br /> 2051 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG <br /> 2101 CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT <br /> 2151 CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT <br /> 2201 TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC <br /> 2251 GCCGGCATGG CGGCCCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG <br /> 2301 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC <br /> 2351 CGATACGCGA GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC <br /> 2401 TGAGCAACAA CATGAATGGT CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA <br /> 2451 AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC TGCATCGCAG <br /> 2501 GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC <br /> 2551 TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC <br /> 2601 CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG <br /> 2651 TAACCCGTAT CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC <br /> 2701 ATGAACAGAA ATCCCCCTTA CACGGAGGCA TCAGTGACCA AACAGGAAAA <br /> 2751 AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA TTAACGCTTC <br /> 2801 TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA <br /> 2851 TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT <br /> 2901 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA <br /> 2951 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG <br /> 3001 TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG <br /> 3051 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT <br /> 3101 ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG <br /> 3151 GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC <br /> 3201 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG <br /> 3251 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG <br /> 3301 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG <br /> 3351 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC <br /> 3401 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT <br /> 3451 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA <br /> 3501 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT <br /> 3551 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT <br /> 3601 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG <br /> 3651 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG <br /> 3701 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC <br /> 3751 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG <br /> 3801 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT <br /> 3851 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT <br /> 3901 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC <br /> 3951 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT <br /> 4001 TAAGGGATTT TGGTCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA <br /> 4051 ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC TTGCTCTAGG <br /> 4101 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC <br /> 4151 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA <br /> 4201 AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC <br /> 4251 AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT <br /> 4301 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT <br /> 4351 TACTCACCAC TGCGATCCCC GGGAAAACAG CATTCCAGGT ATTAGAAGAA <br /> 4401 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG <br /> 4451 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG <br /> 4501 TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT <br /> 4551 GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG <br /> 4601 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC <br /> 4651 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA <br /> 4701 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT <br /> 4751 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC <br /> 4801 GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG <br /> 4851 TTTCATTTGA TGCTCGATGA GTTTTTCTAA GAATTAATTC ATGAGCGGAT <br /> 4901 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA <br /> 4951 TTTCCCCGAA AAGTGCCACC TGAAATTGTA AACGTTAATA TTTTGTTAAA <br /> 5001 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG <br /> 5051 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG <br /> 5101 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC <br /> 5151 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG <br /> 5201 AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA <br /> 5251 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC <br /> 5301 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA <br /> 5351 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC <br /> 5401 GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCA<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=File:Pet-30eklic.JPG&diff=316614 File:Pet-30eklic.JPG 2007-10-05T12:25:01Z <p>Charner: </p> <hr /> <div></div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-30_Ek/LIC&diff=316613 pET-30 Ek/LIC 2007-10-05T12:22:50Z <p>Charner: /* Sequence {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69077-000.HTM Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> 1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA <br /> 51 GAGGCCCCAA GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA <br /> 101 CTCAGCTTCC TTTCGGGCTT TGTTAGCAGC CGGATCTCAG TGGTGGTGGT <br /> 151 GGTGGTGCTC GAGTGCGGCC GCAAGCTTGT CGACGGAGCT CGAATTCGGA <br /> 201 TCCGATATCG CCATGGTTGA GGAGAAGCCC GGGCTCTTGT CGTCGTCATC <br /> 251 GGTACCCAGA TCTGGGCTGT CCATGTGCTG GCGTTCGAAT TTAGCAGCAG <br /> 301 CGGTTTCTTT CATACCAGAA CCGCGTGGCA CCAGACCAGA AGAATGATGA <br /> 351 TGATGATGGT GCATATGTAT ATCTCCTTCT TAAAGTTAAA CAAAATTATT <br /> 401 TCTAGAGGGG AATTGTTATC CGCTCACAAT TCCCCTATAG TGAGTCGTAT <br /> 451 TAATTTCGCG GGATCGAGAT CGATCTCGAT CCTCTACGCC GGACGCATCG <br /> 501 TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC <br /> 551 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC <br /> 601 TTGTTTCGGC GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG <br /> 651 GCGCCATCTC CTTGCATGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC <br /> 701 CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA <br /> 751 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG <br /> 801 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC <br /> 851 AGTAACGTTA TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG <br /> 901 TTTCCCGCGT GGTGAACCAG GCCAGCCACG TTTCTGCGAA AACGCGGGAA <br /> 951 AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA ACCGCGTGGC <br /> 1001 ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA <br /> 1051 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC <br /> 1101 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG <br /> 1151 CGTCGAAGCC TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA <br /> 1201 GTGGGCTGAT CATTAACTAT CCGCTGGATG ACCAGGATGC CATTGCTGTG <br /> 1251 GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG TCTCTGACCA <br /> 1301 GACACCCATC AACAGTATTA TTTTCTCCCA TGAAGACGGT ACGCGACTGG <br /> 1351 GCGTGGAGCA TCTGGTCGCA TTGGGTCACC AGCAAATCGC GCTGTTAGCG <br /> 1401 GGCCCATTAA GTTCTGTCTC GGCGCGTCTG CGTCTGGCTG GCTGGCATAA <br /> 1451 ATATCTCACT CGCAATCAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT <br /> 1501 GGAGTGCCAT GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC <br /> 1551 ATCGTTCCCA CTGCGATGCT GGTTGCCAAC GATCAGATGG CGCTGGGCGC <br /> 1601 AATGCGCGCC ATTACCGAGT CCGGGCTGCG CGTTGGTGCG GACATCTCGG <br /> 1651 TAGTGGGATA CGACGATACC GAAGACAGCT CATGTTATAT CCCGCCGTTA <br /> 1701 ACCACCATCA AACAGGATTT TCGCCTGCTG GGGCAAACCA GCGTGGACCG <br /> 1751 CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC <br /> 1801 CCGTCTCACT GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC <br /> 1851 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT <br /> 1901 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG <br /> 1951 CTCACTCATT AGGCACCGGG ATCTCGACCG ATGCCCTTGA GAGCCTTCAA <br /> 2001 CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC <br /> 2051 TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG <br /> 2101 CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT <br /> 2151 CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT <br /> 2201 TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC <br /> 2251 GCCGGCATGG CGGCCCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG <br /> 2301 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC <br /> 2351 CGATACGCGA GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC <br /> 2401 TGAGCAACAA CATGAATGGT CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA <br /> 2451 AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC TGCATCGCAG <br /> 2501 GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC <br /> 2551 TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC <br /> 2601 CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG <br /> 2651 TAACCCGTAT CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC <br /> 2701 ATGAACAGAA ATCCCCCTTA CACGGAGGCA TCAGTGACCA AACAGGAAAA <br /> 2751 AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA TTAACGCTTC <br /> 2801 TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA <br /> 2851 TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT <br /> 2901 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA <br /> 2951 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG <br /> 3001 TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG <br /> 3051 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT <br /> 3101 ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG <br /> 3151 GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC <br /> 3201 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG <br /> 3251 GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG <br /> 3301 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG <br /> 3351 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC <br /> 3401 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT <br /> 3451 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA <br /> 3501 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT <br /> 3551 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT <br /> 3601 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG <br /> 3651 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG <br /> 3701 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC <br /> 3751 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG <br /> 3801 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT <br /> 3851 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT <br /> 3901 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC <br /> 3951 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT <br /> 4001 TAAGGGATTT TGGTCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA <br /> 4051 ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC TTGCTCTAGG <br /> 4101 CCGCGATTAA ATTCCAACAT GGATGCTGAT TTATATGGGT ATAAATGGGC <br /> 4151 TCGCGATAAT GTCGGGCAAT CAGGTGCGAC AATCTATCGA TTGTATGGGA <br /> 4201 AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG TAGCGTTGCC <br /> 4251 AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT <br /> 4301 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT <br /> 4351 TACTCACCAC TGCGATCCCC GGGAAAACAG CATTCCAGGT ATTAGAAGAA <br /> 4401 TATCCTGATT CAGGTGAAAA TATTGTTGAT GCGCTGGCAG TGTTCCTGCG <br /> 4451 CCGGTTGCAT TCGATTCCTG TTTGTAATTG TCCTTTTAAC AGCGATCGCG <br /> 4501 TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG TTTGGTTGAT <br /> 4551 GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG <br /> 4601 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC <br /> 4651 ATGGTGATTT CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA <br /> 4701 GGTTGTATTG ATGTTGGACG AGTCGGAATC GCAGACCGAT ACCAGGATCT <br /> 4751 TGCCATCCTA TGGAACTGCC TCGGTGAGTT TTCTCCTTCA TTACAGAAAC <br /> 4801 GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA TAAATTGCAG <br /> 4851 TTTCATTTGA TGCTCGATGA GTTTTTCTAA GAATTAATTC ATGAGCGGAT <br /> 4901 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA <br /> 4951 TTTCCCCGAA AAGTGCCACC TGAAATTGTA AACGTTAATA TTTTGTTAAA <br /> 5001 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG <br /> 5051 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG <br /> 5101 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC <br /> 5151 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG <br /> 5201 AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA <br /> 5251 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC <br /> 5301 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA <br /> 5351 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC <br /> 5401 GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCA<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-30_Ek/LIC&diff=316612 pET-30 Ek/LIC 2007-10-05T12:21:37Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69077-000.HTM Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-30_Ek/LIC&diff=316611 pET-30 Ek/LIC 2007-10-05T12:19:31Z <p>Charner: New page: ==At a Glance {{HelpIcon|Help:Plasmid}}== &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt; &lt;!-- *******************************************************************...</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |-<br /> !{{table heading style}} |Copy #<br /> |-<br /> !{{table heading style}} |Markers<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |-<br /> !{{table heading style}} |Comments<br /> |-<br /> |colspan='1'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.L47062bd323465--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=EcoliWiki:Tracking_Plasmids&diff=316610 EcoliWiki:Tracking Plasmids 2007-10-05T11:59:58Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>This is a page to track plasmids entered into the wiki. It is for developmental purposes only. <br /> <br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|class = 'sortable' {{Prettytable}} id='220572' <br /> |-<br /> !Plasmid Name!!Company!!Assigned To!!Date Entered!!Reviewed By!!Date Reviewed<br /> |-<br /> |<br /> LITMUS 28i<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.03.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.03.07 <br /> |-<br /> |<br /> LITMUS 38i<br /> |<br /> NEB<br /> |<br /> Miriam Garcia<br /> |<br /> 08.31.2007<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGPS1.1<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 08.31.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.04.07 <br /> |-<br /> |<br /> pGPS2.1<br /> |<br /> NEB<br /> |<br /> Matthew Lakich<br /> |<br /> 09.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.06.07 <br /> |-<br /> |<br /> pGPS3<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |-<br /> |<br /> pGPS4<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 09.03.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.04.07 <br /> |-<br /> |<br /> pGPS5<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.03.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.04.07 <br /> |-<br /> |<br /> pMAL-c4E<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |-<br /> |<br /> pMAL-c4G<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |-<br /> |<br /> pMAL-c4X<br /> |<br /> NEB<br /> |<br /> Matthew Lakich<br /> |<br /> 9.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pMAL-p4E<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.07.07 <br /> |-<br /> |<br /> pMAL-p4G<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pMAL-p4X<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pMAL-pIII<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pNEB193<br /> |<br /> NEB<br /> |<br /> CharnerWilliams<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pNEB206A<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pTWIN1<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pTXB1<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pTXB3<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |-<br /> |<br /> pTYB1<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pTYB11<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pUC19<br /> |<br /> NEB<br /> |<br /> <br /> |<br /> <br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pNEBR-R1<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |-<br /> |<br /> pNEBR-X1<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |-<br /> |<br /> pNEBR-X1GLuc<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |-<br /> |<br /> pNEBR-X1Hygro<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.10.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |-<br /> |<br /> pKLAC1 Vector<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |-<br /> |<br /> pKLAC1-malE Control Plasmid<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |-<br /> |<br /> pCMV-GLuc Control Plasmid<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |-<br /> |<br /> pGLuc-Basic Vector<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |-<br /> |<br /> pBEc-Q<br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pBEc-SBP <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pBEc-SBP-Q<br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pBEc-SBP-SET1 <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pBEc-SBP-SET1-Q<br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pBEc-SBP-SET2 <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pBEc-SBP-SET2-Q<br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pBEc-SBP-SET3<br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pBEc-SBP-SET3-Q<br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pBEc-SET1 <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |-<br /> |<br /> pBEc-SET2 <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |-<br /> |<br /> pBEc-SET3 <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |-<br /> |<br /> pBEn-SBP-SET1a <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |-<br /> |<br /> pBEn-SBP-SET1b <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |-<br /> |<br /> pBEn-SBP-SET1c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |-<br /> |<br /> pBEn-SBP-SET2a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |-<br /> |<br /> pBEn-SBP-SET2b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SBP-SET2c<br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SBP-SET3a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SBP-SET3b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SBP-SET3c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SBPa <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pBEn-SBPb <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pBEn-SBPc <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pBEn-SET1a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.24.07 <br /> |-<br /> |<br /> pBEn-SET1b<br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET1c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET2a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET2b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET2c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET3a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET3b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET3c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pCAL-c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pCAL-kc <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pCAL-n <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pCAL-n-EK <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |-<br /> |<br /> pCAL-n-FLAG <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |-<br /> |<br /> pET-11a <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |-<br /> |<br /> pET-11b <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |-<br /> |<br /> pET-11c <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |-<br /> |<br /> pET-11d <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |-<br /> |<br /> pET-3a <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.17.07 <br /> |-<br /> |<br /> pET-3b <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.17.07 <br /> |-<br /> |<br /> pET-3c <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.17.07 <br /> |-<br /> |<br /> pET-3d <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.17.07 <br /> |-<br /> |<br /> pET100/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-14b<br /> |<br /> Novagen<br /> |<br /> Miriam Garcia<br /> |<br /> 09.19.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-15b <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |-<br /> |<br /> pET-16b<br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 9.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |-<br /> |<br /> pET-17b <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 9.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |-<br /> |<br /> pET-19b <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 9.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |-<br /> |<br /> pET-20b(+) <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-21(+) <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-21a(+) <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.2.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET-21b(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.24.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-21c(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.24.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.25.07 <br /> |-<br /> |<br /> pET-21d(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-22b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-23(+)<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-23a(+) <br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-23b(+)<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-23c(+) <br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-23d(+) <br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia <br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-24(+) <br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia <br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-24a(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-24b(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-24c(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-24d(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-25b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 9.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-26b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 9.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-27b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 9.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-28a(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-28b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-28c(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-29a(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-29b(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-29c(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-30a(+) <br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-30b(+)<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-30c(+) <br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-31b(+) DNA<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-32a(+) DNA<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-32b(+) DNA<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-32c(+) DNA<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-33b(+)<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-39b(+)<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-40b(+) <br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-41a(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-41b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-41c(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-42a(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-42b(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-42c(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-43.1a(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-43.1b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-43.1c(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-44a(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-44b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-44c(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-45b(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-47b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-48b(+)<br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.3.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-49b(+)<br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.3.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-50b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET-51b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET-52b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET-9a <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-9b<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-9c <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-9d<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pETcoco-1 <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pETcoco-2<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pLacI <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pLysE <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pLysS<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET100/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.3.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET101/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET101/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET102/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET102/D-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET151/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET151/D-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET160-GW/CAT <br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET160/GW/D-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET161-GW/CAT <br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET161/GW/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET200/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET200/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-DEST42<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pDEST15 <br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pDEST14 <br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pDEST17 <br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pDEST24<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.04.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pRSET T7<br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pRSET-E <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCRT7-E <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD102/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD102/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD202/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD202/D-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/Thio <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/Thio-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD-TOPO/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pBAD-TOPO/LacZ/V5-His <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pBAD-DEST49 <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pBAD/His/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pBAD/His A<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/His B<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/His C<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/myc-His A<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/myc-His B<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/myc-His C<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/Myc-His/lacZ<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/gIII Calmodulin<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/gIII A<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/gIII B<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/gIII C<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/Thio-E<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pThioHis A<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pThioHis B<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pThioHis C<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis-TOPO <br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis-TOPO/lacZ<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2-TOPO <br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2-TOPO/LacZ<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis/CAT<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis A<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis B<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis C<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2/LacZ<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2 A<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2 B<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2 C<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCX <br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCX-TOPO <br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-30 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-32 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-41 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-43.1 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-44 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-46 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-51 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCDF-2 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pRSF-2 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAC-2cp<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBACgus-2cp<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-30 Xa/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-32 Xa/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-52 3c/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pETDuet-1<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pACYCDuet-1<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCOLADuet-1<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCDFDuet-1<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pRSFDuet-1<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pETBlue-1<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pETBlue-2<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBiEx-1<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBiEx-2<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBiEx-3<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF1A T7<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF1K T7<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN2A (GST)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN2K (GST)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF3A WG (BYDV)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF3K WG (BYDV)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF4A CMV<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF4K CMV<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF5A CMV-neo<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF5K CMV-neo<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN6A (HQ)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN6K (HQ)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFC7A (HQ)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFC7A (HQ)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFC8A (HaloTag)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFC8K (HaloTag)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF9A CMV hRluc-neo<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN10A (ACT)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN11A (BIND)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF12A RM<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF12K RM<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Xa-1 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pALTER-EX1 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pALTER-EX2<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-5a<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-5b<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-5c<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEMEX-1 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEMEX-2<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Control <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Xa Control <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Xa-2 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Xa-3 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT3-Basic<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT3-control<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT3-Enhancer<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT3-Promoter<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-3Z<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-3Zf(+)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-3Zf(-)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-4Z <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-5Zf(+) <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-5Zf(-)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-7Zf(+) <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-7Zf(-)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-9Zf(-)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-11Zf(+)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-11Zf(-) <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-13Zf(+)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL2-Basic <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL2-Control <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL2-Enhancer<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL2-Promoter<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pG5luc<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL3-Basic<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL3-Control <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL3-Enhancer<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL3-Promoter<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP64 Poly(A) <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP72 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP73 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT Basic<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT Control<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT Enhancer<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT Promoter<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-1<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-2 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-3 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-4 Vector <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-15Zf(-)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP64 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP65<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP70<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-class='sortbottom'<br /> |[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table] || || || || ||<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129--&gt;&lt;/protect&gt;</div> Charner https://ecoliwiki.org/colipedia/index.php?title=EcoliWiki:Tracking_Plasmids&diff=316609 EcoliWiki:Tracking Plasmids 2007-10-05T11:54:45Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>This is a page to track plasmids entered into the wiki. It is for developmental purposes only. <br /> <br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|class = 'sortable' {{Prettytable}} id='220572' <br /> |-<br /> !Plasmid Name!!Company!!Assigned To!!Date Entered!!Reviewed By!!Date Reviewed<br /> |-<br /> |<br /> LITMUS 28i<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.03.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.03.07 <br /> |-<br /> |<br /> LITMUS 38i<br /> |<br /> NEB<br /> |<br /> Miriam Garcia<br /> |<br /> 08.31.2007<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGPS1.1<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 08.31.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.04.07 <br /> |-<br /> |<br /> pGPS2.1<br /> |<br /> NEB<br /> |<br /> Matthew Lakich<br /> |<br /> 09.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.06.07 <br /> |-<br /> |<br /> pGPS3<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |-<br /> |<br /> pGPS4<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 09.03.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.04.07 <br /> |-<br /> |<br /> pGPS5<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.03.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.04.07 <br /> |-<br /> |<br /> pMAL-c4E<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |-<br /> |<br /> pMAL-c4G<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |-<br /> |<br /> pMAL-c4X<br /> |<br /> NEB<br /> |<br /> Matthew Lakich<br /> |<br /> 9.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pMAL-p4E<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.07.07 <br /> |-<br /> |<br /> pMAL-p4G<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pMAL-p4X<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pMAL-pIII<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pNEB193<br /> |<br /> NEB<br /> |<br /> CharnerWilliams<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pNEB206A<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pTWIN1<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pTXB1<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pTXB3<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |-<br /> |<br /> pTYB1<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pTYB11<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pUC19<br /> |<br /> NEB<br /> |<br /> <br /> |<br /> <br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pNEBR-R1<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |-<br /> |<br /> pNEBR-X1<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |-<br /> |<br /> pNEBR-X1GLuc<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |-<br /> |<br /> pNEBR-X1Hygro<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.10.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |-<br /> |<br /> pKLAC1 Vector<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |-<br /> |<br /> pKLAC1-malE Control Plasmid<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |-<br /> |<br /> pCMV-GLuc Control Plasmid<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |-<br /> |<br /> pGLuc-Basic Vector<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |-<br /> |<br /> pBEc-Q<br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pBEc-SBP <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pBEc-SBP-Q<br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pBEc-SBP-SET1 <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pBEc-SBP-SET1-Q<br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pBEc-SBP-SET2 <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pBEc-SBP-SET2-Q<br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |-<br /> |<br /> pBEc-SBP-SET3<br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pBEc-SBP-SET3-Q<br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pBEc-SET1 <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |-<br /> |<br /> pBEc-SET2 <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |-<br /> |<br /> pBEc-SET3 <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |-<br /> |<br /> pBEn-SBP-SET1a <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |-<br /> |<br /> pBEn-SBP-SET1b <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |-<br /> |<br /> pBEn-SBP-SET1c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |-<br /> |<br /> pBEn-SBP-SET2a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |-<br /> |<br /> pBEn-SBP-SET2b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SBP-SET2c<br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SBP-SET3a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SBP-SET3b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SBP-SET3c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SBPa <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pBEn-SBPb <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pBEn-SBPc <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |-<br /> |<br /> pBEn-SET1a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.24.07 <br /> |-<br /> |<br /> pBEn-SET1b<br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET1c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET2a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET2b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET2c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET3a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET3b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pBEn-SET3c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pCAL-c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pCAL-kc <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pCAL-n <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |-<br /> |<br /> pCAL-n-EK <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |-<br /> |<br /> pCAL-n-FLAG <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |-<br /> |<br /> pET-11a <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |-<br /> |<br /> pET-11b <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |-<br /> |<br /> pET-11c <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |-<br /> |<br /> pET-11d <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |-<br /> |<br /> pET-3a <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.17.07 <br /> |-<br /> |<br /> pET-3b <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.17.07 <br /> |-<br /> |<br /> pET-3c <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.17.07 <br /> |-<br /> |<br /> pET-3d <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.17.07 <br /> |-<br /> |<br /> pET100/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-14b<br /> |<br /> Novagen<br /> |<br /> Miriam Garcia<br /> |<br /> 09.19.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-15b <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |-<br /> |<br /> pET-16b<br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 9.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |-<br /> |<br /> pET-17b <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 9.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |-<br /> |<br /> pET-19b <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 9.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |-<br /> |<br /> pET-20b(+) <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-21(+) <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-21a(+) <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.2.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET-21b(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.24.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-21c(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.24.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.25.07 <br /> |-<br /> |<br /> pET-21d(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-22b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-23(+)<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-23a(+) <br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-23b(+)<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-23c(+) <br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-23d(+) <br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia <br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-24(+) <br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia <br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-24a(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-24b(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-24c(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-24d(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |-<br /> |<br /> pET-25b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 9.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-26b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 9.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-27b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 9.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-28a(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-28b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-28c(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-29a(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-29b(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-29c(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-30a(+) <br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-30b(+)<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-30c(+) <br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-31b(+) DNA<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-32a(+) DNA<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-32b(+) DNA<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-32c(+) DNA<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-33b(+)<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-39b(+)<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-40b(+) <br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-41a(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-41b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-41c(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-42a(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-42b(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-42c(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |-<br /> |<br /> pET-43.1a(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-43.1b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-43.1c(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-44a(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-44b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-44c(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-45b(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-47b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-48b(+)<br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.3.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-49b(+)<br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.3.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET-50b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET-51b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET-52b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET-9a <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-9b<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-9c <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pET-9d<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |-<br /> |<br /> pETcoco-1 <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pETcoco-2<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pLacI <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pLysE <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pLysS<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET100/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.3.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET101/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET101/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET102/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pET102/D-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET151/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET151/D-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |-<br /> |<br /> pET160-GW/CAT <br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET160/GW/D-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET161-GW/CAT <br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET161/GW/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET200/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET200/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-DEST42<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pDEST15 <br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pDEST14 <br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pDEST17 <br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pDEST24<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.04.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pRSET T7<br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pRSET-E <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCRT7-E <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD102/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD102/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD202/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD202/D-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/Thio <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/Thio-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD-TOPO/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pBAD-TOPO/LacZ/V5-His <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pBAD-DEST49 <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pBAD/His/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |-<br /> |<br /> pBAD/His A<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/His B<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/His C<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/myc-His A<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/myc-His B<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/myc-His C<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/Myc-His/lacZ<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/gIII Calmodulin<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/gIII A<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/gIII B<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/gIII C<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/Thio-E<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pThioHis A<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pThioHis B<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pThioHis C<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis-TOPO <br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis-TOPO/lacZ<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2-TOPO <br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2-TOPO/LacZ<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis/CAT<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis A<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis B<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis C<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2/LacZ<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2 A<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2 B<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2 C<br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCX <br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCX-TOPO <br /> |<br /> Invitrogen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-30 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-32 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-41 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-43.1 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-44 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-46 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-51 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCDF-2 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pRSF-2 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAC-2cp<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBACgus-2cp<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-30 Xa/LIC<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-32 Xa/LIC<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-52 3c/LIC<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pETDuet-1<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pACYCDuet-1<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCOLADuet-1<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCDFDuet-1<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pRSFDuet-1<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pETBlue-1<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pETBlue-2<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBiEx-1<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBiEx-2<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBiEx-3<br /> |<br /> Novagen<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF1A T7<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF1K T7<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN2A (GST)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN2K (GST)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF3A WG (BYDV)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF3K WG (BYDV)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF4A CMV<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF4K CMV<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF5A CMV-neo<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF5K CMV-neo<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN6A (HQ)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN6K (HQ)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFC7A (HQ)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFC7A (HQ)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFC8A (HaloTag)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFC8K (HaloTag)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF9A CMV hRluc-neo<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN10A (ACT)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN11A (BIND)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF12A RM<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF12K RM<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Xa-1 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pALTER-EX1 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pALTER-EX2<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-5a<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-5b<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-5c<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEMEX-1 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEMEX-2<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Control <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Xa Control <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Xa-2 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Xa-3 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT3-Basic<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT3-control<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT3-Enhancer<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT3-Promoter<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-3Z<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-3Zf(+)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-3Zf(-)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-4Z <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-5Zf(+) <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-5Zf(-)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-7Zf(+) <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-7Zf(-)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-9Zf(-)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-11Zf(+)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-11Zf(-) <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-13Zf(+)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL2-Basic <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL2-Control <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL2-Enhancer<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL2-Promoter<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pG5luc<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL3-Basic<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL3-Control <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL3-Enhancer<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL3-Promoter<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP64 Poly(A) <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP72 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP73 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT Basic<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT Control<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT Enhancer<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAT Promoter<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-1<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-2 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-3 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-4 Vector <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-15Zf(-)<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP64 <br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP65<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP70<br /> |<br /> Promega<br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |<br /> <br /> |-class='sortbottom'<br /> |[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table] || || || || ||<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129--&gt;&lt;/protect&gt;</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-40b(%2B)&diff=316608 pET-40b(+) 2007-10-05T11:38:24Z <p>Charner: /* At a Glance {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-40b%28%2B%29.JPG|right|thumb|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB229.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70091-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-40b(+) vector is designed for expression of DsbC fusion proteins.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt <br /> 181 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg atatcgccat ggttgaggag <br /> 241 aagcccgggc tcttgtcgtc gtcatcggta cccagatctg ggctgtccat gtgctggcgt <br /> 301 tcgaatttag cagcagcggt ttctttcata ccaattgcag tactaccgcg tggcaccaga <br /> 361 cccgcggagt gatggtgatg gtgatgacca gaaccactag ttgatccttt accgctggtc <br /> 421 attttttggt gttcgtcgag aaattctttc atctctttcg gcggctggta acccggaaca <br /> 481 agtgtgccat tgctcagcac aactgccgga gtaccgctaa cgccaagctg gacgccaagt <br /> 541 gcgtaatggt cggcaatatc cacgtcgcaa ctggctggtg cgacgctttt acctgccatc <br /> 601 acatcatcaa acgctttgtt tttatctttc gcacaccaga tagctttcat ttctttctct <br /> 661 gcatcgctgt ccagcccctg gcgcgggaaa gcaagataac gcacggtgat ccccagcgcg <br /> 721 ttgtagtctg ccatttgctc atgcagtttg tggcagtaac cacaggtaat atcagtaaac <br /> 781 acggtgatga cgtgtttttc ctgcggcgct ttataaacga tcatctcttt ttcaagcgca <br /> 841 ttcaactgct ttaacagcat cttattggtg acattgaccg gagccgtgcc actaacgtca <br /> 901 tacattggcc cctgaatgat atgtttacca tcatcggtga tgtacaacac gccgctgtta <br /> 961 gtcagaactg tcttcatgcc agctacaggc gcgggctgaa tatcgctgct tttgatgccc <br /> 1021 attttggcta acgtttgttg aattgccgcg tcatcagcct gagcaaagcc tgaaaacgcc <br /> 1081 gctaacaaag taaacaacat aaaacctttc ttcatatgta tatctccttc ttaaagttaa <br /> 1141 acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata gtgagtcgta <br /> 1201 ttaatttcgc gggatcgaga tcgatctcga tcctctacgc cggacgcatc gtggccggca <br /> 1261 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag <br /> 1321 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc <br /> 1381 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg <br /> 1441 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag <br /> 1501 agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag <br /> 1561 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc <br /> 1621 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac <br /> 1681 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc <br /> 1741 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc <br /> 1801 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa <br /> 1861 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg <br /> 1921 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat <br /> 1981 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat <br /> 2041 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg <br /> 2101 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta <br /> 2161 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa <br /> 2221 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc <br /> 2281 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg <br /> 2341 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggacatctcg <br /> 2401 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc <br /> 2461 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag <br /> 2521 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc <br /> 2581 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg <br /> 2641 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta <br /> 2701 gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca acccagtcag <br /> 2761 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat <br /> 2821 catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt <br /> 2881 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct <br /> 2941 cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat <br /> 3001 cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct <br /> 3061 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg <br /> 3121 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt <br /> 3181 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat <br /> 3241 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg <br /> 3301 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt <br /> 3361 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat <br /> 3421 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc <br /> 3481 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac <br /> 3541 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga <br /> 3601 atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga <br /> 3661 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga <br /> 3721 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc <br /> 3781 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca <br /> 3841 gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac agatgcgtaa <br /> 3901 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg <br /> 3961 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag <br /> 4021 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc <br /> 4081 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca <br /> 4141 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt <br /> 4201 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc <br /> 4261 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc <br /> 4321 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc <br /> 4381 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact <br /> 4441 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg <br /> 4501 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta <br /> 4561 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca <br /> 4621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa <br /> 4681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg <br /> 4741 aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta cataaacagt <br /> 4801 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag gccgcgatta <br /> 4861 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa <br /> 4921 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa <br /> 4981 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg <br /> 5041 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg <br /> 5101 ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat <br /> 5161 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct <br /> 5221 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga <br /> 5281 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt <br /> 5341 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact <br /> 5401 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt <br /> 5461 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc <br /> 5521 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat <br /> 5581 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta agaattaatt <br /> 5641 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac <br /> 5701 atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt <br /> 5761 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta <br /> 5821 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc <br /> 5881 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg <br /> 5941 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact <br /> 6001 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt <br /> 6061 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc <br /> 6121 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc <br /> 6181 ccattcgcca <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=File:Pet-40b(%2B).JPG&diff=316607 File:Pet-40b(+).JPG 2007-10-05T11:37:30Z <p>Charner: </p> <hr /> <div></div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-40b(%2B)&diff=316606 pET-40b(+) 2007-10-05T11:36:29Z <p>Charner: /* Sequence {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70091-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-40b(+) vector is designed for expression of DsbC fusion proteins.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt <br /> 181 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg atatcgccat ggttgaggag <br /> 241 aagcccgggc tcttgtcgtc gtcatcggta cccagatctg ggctgtccat gtgctggcgt <br /> 301 tcgaatttag cagcagcggt ttctttcata ccaattgcag tactaccgcg tggcaccaga <br /> 361 cccgcggagt gatggtgatg gtgatgacca gaaccactag ttgatccttt accgctggtc <br /> 421 attttttggt gttcgtcgag aaattctttc atctctttcg gcggctggta acccggaaca <br /> 481 agtgtgccat tgctcagcac aactgccgga gtaccgctaa cgccaagctg gacgccaagt <br /> 541 gcgtaatggt cggcaatatc cacgtcgcaa ctggctggtg cgacgctttt acctgccatc <br /> 601 acatcatcaa acgctttgtt tttatctttc gcacaccaga tagctttcat ttctttctct <br /> 661 gcatcgctgt ccagcccctg gcgcgggaaa gcaagataac gcacggtgat ccccagcgcg <br /> 721 ttgtagtctg ccatttgctc atgcagtttg tggcagtaac cacaggtaat atcagtaaac <br /> 781 acggtgatga cgtgtttttc ctgcggcgct ttataaacga tcatctcttt ttcaagcgca <br /> 841 ttcaactgct ttaacagcat cttattggtg acattgaccg gagccgtgcc actaacgtca <br /> 901 tacattggcc cctgaatgat atgtttacca tcatcggtga tgtacaacac gccgctgtta <br /> 961 gtcagaactg tcttcatgcc agctacaggc gcgggctgaa tatcgctgct tttgatgccc <br /> 1021 attttggcta acgtttgttg aattgccgcg tcatcagcct gagcaaagcc tgaaaacgcc <br /> 1081 gctaacaaag taaacaacat aaaacctttc ttcatatgta tatctccttc ttaaagttaa <br /> 1141 acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata gtgagtcgta <br /> 1201 ttaatttcgc gggatcgaga tcgatctcga tcctctacgc cggacgcatc gtggccggca <br /> 1261 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag <br /> 1321 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc <br /> 1381 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg <br /> 1441 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag <br /> 1501 agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag <br /> 1561 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc <br /> 1621 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac <br /> 1681 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc <br /> 1741 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc <br /> 1801 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa <br /> 1861 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg <br /> 1921 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat <br /> 1981 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat <br /> 2041 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg <br /> 2101 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta <br /> 2161 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa <br /> 2221 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc <br /> 2281 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg <br /> 2341 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggacatctcg <br /> 2401 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc <br /> 2461 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag <br /> 2521 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc <br /> 2581 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg <br /> 2641 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta <br /> 2701 gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca acccagtcag <br /> 2761 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat <br /> 2821 catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt <br /> 2881 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct <br /> 2941 cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat <br /> 3001 cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct <br /> 3061 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg <br /> 3121 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt <br /> 3181 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat <br /> 3241 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg <br /> 3301 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt <br /> 3361 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat <br /> 3421 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc <br /> 3481 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac <br /> 3541 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga <br /> 3601 atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga <br /> 3661 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga <br /> 3721 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc <br /> 3781 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca <br /> 3841 gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac agatgcgtaa <br /> 3901 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg <br /> 3961 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag <br /> 4021 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc <br /> 4081 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca <br /> 4141 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt <br /> 4201 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc <br /> 4261 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc <br /> 4321 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc <br /> 4381 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact <br /> 4441 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg <br /> 4501 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta <br /> 4561 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca <br /> 4621 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa <br /> 4681 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg <br /> 4741 aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta cataaacagt <br /> 4801 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag gccgcgatta <br /> 4861 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa <br /> 4921 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa <br /> 4981 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg <br /> 5041 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg <br /> 5101 ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat <br /> 5161 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct <br /> 5221 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga <br /> 5281 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt <br /> 5341 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact <br /> 5401 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt <br /> 5461 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc <br /> 5521 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat <br /> 5581 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta agaattaatt <br /> 5641 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac <br /> 5701 atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt <br /> 5761 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta <br /> 5821 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc <br /> 5881 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg <br /> 5941 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact <br /> 6001 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt <br /> 6061 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc <br /> 6121 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc <br /> 6181 ccattcgcca <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-40b(%2B)&diff=316605 pET-40b(+) 2007-10-05T11:36:02Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70091-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-40b(+) vector is designed for expression of DsbC fusion proteins.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-40b(%2B)&diff=316604 pET-40b(+) 2007-10-05T11:34:14Z <p>Charner: New page: ==At a Glance {{HelpIcon|Help:Plasmid}}== &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca--&gt; &lt;!-- *******************************************************************...</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |-<br /> !{{table heading style}} |Copy #<br /> |-<br /> !{{table heading style}} |Markers<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |-<br /> !{{table heading style}} |Comments<br /> |-<br /> |colspan='1'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47062135af7ca--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-39b(%2B)&diff=316603 pET-39b(+) 2007-10-05T11:33:34Z <p>Charner: /* References */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-39b%28%2B%29.JPG|thumb|right|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB230.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70090-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-39b(+) vector is designed for expression of DsbA fusion proteins. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> <br /> &lt;ref name='PMID: 17403869'/&gt;<br /> <br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt <br /> 181 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg atatcgccat ggttgaggag <br /> 241 aagcccgggc tcttgtcgtc gtcatcggta cccagatctg ggctgtccat gtgctggcgt <br /> 301 tcgaatttag cagcagcggt ttctttcata ccaattgcag tactaccgcg tggcaccaga <br /> 361 cccgcggagt gatggtgatg gtgatgacca gaaccactag ttgatccttt tttctcgctt <br /> 421 aagtatttca ctgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata <br /> 481 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacgcc acgcaattgc <br /> 541 acgtcagctg cagctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac <br /> 601 gccgcgtcgt actcttcacc tttaatacct gcgttgataa atacatcgcg gatatcagaa <br /> 661 gcagaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct <br /> 721 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca <br /> 781 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca <br /> 841 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa <br /> 901 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca <br /> 961 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa <br /> 1021 atctttttca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat <br /> 1081 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatcga <br /> 1141 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg <br /> 1201 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca <br /> 1261 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg <br /> 1321 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac <br /> 1381 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc <br /> 1441 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg <br /> 1501 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat <br /> 1561 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa <br /> 1621 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg <br /> 1681 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg <br /> 1741 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg <br /> 1801 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa <br /> 1861 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa <br /> 1921 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac <br /> 1981 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg <br /> 2041 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt <br /> 2101 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa <br /> 2161 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc <br /> 2221 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt <br /> 2281 accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga cgataccgaa <br /> 2341 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg <br /> 2401 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag <br /> 2461 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc <br /> 2521 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa <br /> 2581 agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc <br /> 2641 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat <br /> 2701 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc <br /> 2761 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg <br /> 2821 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc <br /> 2881 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccccacgggt <br /> 2941 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg <br /> 3001 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc <br /> 3061 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac <br /> 3121 gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct <br /> 3181 accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt <br /> 3241 tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg <br /> 3301 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat <br /> 3361 tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac <br /> 3421 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa <br /> 3481 cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga <br /> 3541 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca <br /> 3601 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca <br /> 3661 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga <br /> 3721 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac <br /> 3781 catatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct <br /> 3841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat <br /> 3901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga <br /> 3961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt <br /> 4021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt <br /> 4081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc <br /> 4141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa <br /> 4201 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct <br /> 4261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta <br /> 4321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg <br /> 4381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc <br /> 4441 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta <br /> 4501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg <br /> 4561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt <br /> 4621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg <br /> 4681 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat <br /> 4741 attcaacggg aaacgtcttg ctctaggccg cgattaaatt ccaacatgga tgctgattta <br /> 4801 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg <br /> 4861 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat <br /> 4921 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc <br /> 4981 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg <br /> 5041 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg <br /> 5101 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc <br /> 5161 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg <br /> 5221 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat <br /> 5281 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac <br /> 5341 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca <br /> 5401 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta <br /> 5461 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt <br /> 5521 catttgatgc tcgatgagtt tttctaagaa ttaattcatg agcggataca tatttgaatg <br /> 5581 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga <br /> 5641 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt <br /> 5701 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat <br /> 5761 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa <br /> 5821 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta <br /> 5881 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc <br /> 5941 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc <br /> 6001 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac <br /> 6061 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-39b(%2B)&diff=316602 pET-39b(+) 2007-10-05T11:32:18Z <p>Charner: /* At a Glance {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-39b%28%2B%29.JPG|thumb|right|300px|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB230.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70090-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-39b(+) vector is designed for expression of DsbA fusion proteins. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt <br /> 181 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg atatcgccat ggttgaggag <br /> 241 aagcccgggc tcttgtcgtc gtcatcggta cccagatctg ggctgtccat gtgctggcgt <br /> 301 tcgaatttag cagcagcggt ttctttcata ccaattgcag tactaccgcg tggcaccaga <br /> 361 cccgcggagt gatggtgatg gtgatgacca gaaccactag ttgatccttt tttctcgctt <br /> 421 aagtatttca ctgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata <br /> 481 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacgcc acgcaattgc <br /> 541 acgtcagctg cagctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac <br /> 601 gccgcgtcgt actcttcacc tttaatacct gcgttgataa atacatcgcg gatatcagaa <br /> 661 gcagaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct <br /> 721 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca <br /> 781 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca <br /> 841 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa <br /> 901 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca <br /> 961 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa <br /> 1021 atctttttca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat <br /> 1081 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatcga <br /> 1141 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg <br /> 1201 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca <br /> 1261 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg <br /> 1321 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac <br /> 1381 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc <br /> 1441 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg <br /> 1501 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat <br /> 1561 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa <br /> 1621 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg <br /> 1681 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg <br /> 1741 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg <br /> 1801 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa <br /> 1861 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa <br /> 1921 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac <br /> 1981 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg <br /> 2041 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt <br /> 2101 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa <br /> 2161 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc <br /> 2221 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt <br /> 2281 accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga cgataccgaa <br /> 2341 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg <br /> 2401 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag <br /> 2461 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc <br /> 2521 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa <br /> 2581 agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc <br /> 2641 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat <br /> 2701 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc <br /> 2761 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg <br /> 2821 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc <br /> 2881 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccccacgggt <br /> 2941 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg <br /> 3001 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc <br /> 3061 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac <br /> 3121 gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct <br /> 3181 accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt <br /> 3241 tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg <br /> 3301 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat <br /> 3361 tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac <br /> 3421 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa <br /> 3481 cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga <br /> 3541 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca <br /> 3601 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca <br /> 3661 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga <br /> 3721 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac <br /> 3781 catatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct <br /> 3841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat <br /> 3901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga <br /> 3961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt <br /> 4021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt <br /> 4081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc <br /> 4141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa <br /> 4201 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct <br /> 4261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta <br /> 4321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg <br /> 4381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc <br /> 4441 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta <br /> 4501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg <br /> 4561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt <br /> 4621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg <br /> 4681 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat <br /> 4741 attcaacggg aaacgtcttg ctctaggccg cgattaaatt ccaacatgga tgctgattta <br /> 4801 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg <br /> 4861 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat <br /> 4921 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc <br /> 4981 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg <br /> 5041 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg <br /> 5101 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc <br /> 5161 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg <br /> 5221 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat <br /> 5281 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac <br /> 5341 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca <br /> 5401 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta <br /> 5461 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt <br /> 5521 catttgatgc tcgatgagtt tttctaagaa ttaattcatg agcggataca tatttgaatg <br /> 5581 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga <br /> 5641 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt <br /> 5701 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat <br /> 5761 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa <br /> 5821 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta <br /> 5881 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc <br /> 5941 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc <br /> 6001 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac <br /> 6061 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=File:Pet-39b(%2B).JPG&diff=316601 File:Pet-39b(+).JPG 2007-10-05T11:30:59Z <p>Charner: </p> <hr /> <div></div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-39b(%2B)&diff=316600 pET-39b(+) 2007-10-05T11:29:56Z <p>Charner: /* Sequence {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70090-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-39b(+) vector is designed for expression of DsbA fusion proteins. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt <br /> 181 gcggccgcaa gcttgtcgac ggagctcgaa ttcggatccg atatcgccat ggttgaggag <br /> 241 aagcccgggc tcttgtcgtc gtcatcggta cccagatctg ggctgtccat gtgctggcgt <br /> 301 tcgaatttag cagcagcggt ttctttcata ccaattgcag tactaccgcg tggcaccaga <br /> 361 cccgcggagt gatggtgatg gtgatgacca gaaccactag ttgatccttt tttctcgctt <br /> 421 aagtatttca ctgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata <br /> 481 ccctgcggat tcagctgata tttaccgtta acaaacatcg ccggaacgcc acgcaattgc <br /> 541 acgtcagctg cagctttttc ctgctgagcg accagagatt tcaccacgaa gctgttccac <br /> 601 gccgcgtcgt actcttcacc tttaatacct gcgttgataa atacatcgcg gatatcagaa <br /> 661 gcagaacgaa tggtctgggt tttctgtacg ccttcaaaca gcggaacagt cactttgtct <br /> 721 tccacgccca gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca <br /> 781 cccatgaagt tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca <br /> 841 ttatcagaaa tatgcagaac ttcttcaaac tgatagcagt gcgggcagaa gaaagagaaa <br /> 901 aactccagca cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca <br /> 961 tcttcatact gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa <br /> 1021 atctttttca tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat <br /> 1081 tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatcga <br /> 1141 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg <br /> 1201 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca <br /> 1261 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg <br /> 1321 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac <br /> 1381 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc <br /> 1441 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg <br /> 1501 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat <br /> 1561 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa <br /> 1621 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg <br /> 1681 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg <br /> 1741 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg <br /> 1801 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa <br /> 1861 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa <br /> 1921 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac <br /> 1981 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg <br /> 2041 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt <br /> 2101 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa <br /> 2161 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc <br /> 2221 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt <br /> 2281 accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga cgataccgaa <br /> 2341 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg <br /> 2401 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag <br /> 2461 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc <br /> 2521 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa <br /> 2581 agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg caccgggatc <br /> 2641 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat <br /> 2701 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc <br /> 2761 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg <br /> 2821 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc <br /> 2881 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccccacgggt <br /> 2941 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg <br /> 3001 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc <br /> 3061 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac <br /> 3121 gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct <br /> 3181 accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt <br /> 3241 tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg <br /> 3301 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat <br /> 3361 tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac <br /> 3421 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa <br /> 3481 cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga <br /> 3541 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca <br /> 3601 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca <br /> 3661 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga <br /> 3721 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac <br /> 3781 catatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct <br /> 3841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat <br /> 3901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga <br /> 3961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt <br /> 4021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt <br /> 4081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc <br /> 4141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa <br /> 4201 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct <br /> 4261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta <br /> 4321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg <br /> 4381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc <br /> 4441 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta <br /> 4501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg <br /> 4561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt <br /> 4621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg <br /> 4681 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat <br /> 4741 attcaacggg aaacgtcttg ctctaggccg cgattaaatt ccaacatgga tgctgattta <br /> 4801 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg <br /> 4861 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat <br /> 4921 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc <br /> 4981 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg <br /> 5041 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg <br /> 5101 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc <br /> 5161 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg <br /> 5221 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat <br /> 5281 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac <br /> 5341 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca <br /> 5401 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta <br /> 5461 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt <br /> 5521 catttgatgc tcgatgagtt tttctaagaa ttaattcatg agcggataca tatttgaatg <br /> 5581 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga <br /> 5641 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt <br /> 5701 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat <br /> 5761 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa <br /> 5821 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta <br /> 5881 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc <br /> 5941 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc <br /> 6001 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac <br /> 6061 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-39b(%2B)&diff=316599 pET-39b(+) 2007-10-05T11:29:18Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70090-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-39b(+) vector is designed for expression of DsbA fusion proteins. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-39b(%2B)&diff=316598 pET-39b(+) 2007-10-05T11:27:36Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-39b(+) vector is designed for expression of DsbA fusion proteins. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-39b(%2B)&diff=316597 pET-39b(+) 2007-10-05T11:25:28Z <p>Charner: New page: ==At a Glance {{HelpIcon|Help:Plasmid}}== &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt; &lt;!-- *******************************************************************...</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |-<br /> !{{table heading style}} |Copy #<br /> |-<br /> !{{table heading style}} |Markers<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |-<br /> !{{table heading style}} |Comments<br /> |-<br /> |colspan='1'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G47061f27c1831--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-33b(%2B)&diff=316596 pET-33b(+) 2007-10-05T11:24:55Z <p>Charner: /* References */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-33b%28%2B%29.JPG|thumb|300px|right|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB147.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69054-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-33b(+) vector is derived from pET-28b(+) and carries a 15 bp sequence encoding the protein kinase A (PKA) site ArgArgAlaSerVal, located between the thrombin cleavage and Nde I sites.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> <br /> &lt;ref name='PMID:16014631'/&gt;<br /> <br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat <br /> 241 gaacagatgc acgacggctg ccgcgcggca ccaggccgct gctgtgatga tgatgatgat <br /> 301 ggctgctgcc catggtatat ctccttctta aagttaaaca aaattatttc tagaggggaa <br /> 361 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatct <br /> 421 cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg <br /> 481 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga <br /> 541 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca <br /> 601 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg <br /> 661 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa <br /> 721 tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg <br /> 781 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag <br /> 841 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg <br /> 901 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc <br /> 961 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa <br /> 1021 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg <br /> 1081 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc <br /> 1141 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct <br /> 1201 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt <br /> 1261 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt <br /> 1321 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg <br /> 1381 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc <br /> 1441 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt <br /> 1501 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc <br /> 1561 gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac <br /> 1621 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa <br /> 1681 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg <br /> 1741 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct <br /> 1801 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc <br /> 1861 gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg <br /> 1921 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac <br /> 1981 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc <br /> 2041 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct <br /> 2101 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc <br /> 2161 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg <br /> 2221 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta <br /> 2281 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc <br /> 2341 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg <br /> 2401 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc <br /> 2461 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct <br /> 2521 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc <br /> 2581 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac <br /> 2641 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc <br /> 2701 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga <br /> 2761 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct <br /> 2821 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct <br /> 2881 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg <br /> 2941 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag <br /> 3001 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat <br /> 3061 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt <br /> 3121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag <br /> 3181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca <br /> 3241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt <br /> 3301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc <br /> 3361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct <br /> 3421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg <br /> 3481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca <br /> 3541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact <br /> 3601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta <br /> 3661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta <br /> 3721 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct <br /> 3781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt <br /> 3841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga <br /> 3901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca <br /> 3961 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt <br /> 4021 caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc tgatttatat <br /> 4081 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat <br /> 4141 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat <br /> 4201 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc <br /> 4261 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa <br /> 4321 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg <br /> 4381 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat <br /> 4441 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt <br /> 4501 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa <br /> 4561 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt <br /> 4621 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac <br /> 4681 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag <br /> 4741 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat <br /> 4801 ttgatgctcg atgagttttt ctaagaatta attcatgagc ggatacatat ttgaatgtat <br /> 4861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat <br /> 4921 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt <br /> 4981 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg <br /> 5041 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt <br /> 5101 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc <br /> 5161 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg <br /> 5221 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa <br /> 5281 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc <br /> 5341 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg cca <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-33b(%2B)&diff=316595 pET-33b(+) 2007-10-05T11:23:29Z <p>Charner: /* At a Glance {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance==<br /> [[Image:Pet-33b%28%2B%29.JPG|thumb|300px|right|Image provided by [http://www.emdbiosciences.com/docs/docs/PROT/TB147.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69054-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-33b(+) vector is derived from pET-28b(+) and carries a 15 bp sequence encoding the protein kinase A (PKA) site ArgArgAlaSerVal, located between the thrombin cleavage and Nde I sites.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat <br /> 241 gaacagatgc acgacggctg ccgcgcggca ccaggccgct gctgtgatga tgatgatgat <br /> 301 ggctgctgcc catggtatat ctccttctta aagttaaaca aaattatttc tagaggggaa <br /> 361 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatct <br /> 421 cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg <br /> 481 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga <br /> 541 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca <br /> 601 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg <br /> 661 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa <br /> 721 tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg <br /> 781 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag <br /> 841 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg <br /> 901 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc <br /> 961 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa <br /> 1021 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg <br /> 1081 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc <br /> 1141 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct <br /> 1201 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt <br /> 1261 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt <br /> 1321 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg <br /> 1381 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc <br /> 1441 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt <br /> 1501 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc <br /> 1561 gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac <br /> 1621 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa <br /> 1681 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg <br /> 1741 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct <br /> 1801 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc <br /> 1861 gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg <br /> 1921 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac <br /> 1981 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc <br /> 2041 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct <br /> 2101 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc <br /> 2161 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg <br /> 2221 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta <br /> 2281 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc <br /> 2341 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg <br /> 2401 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc <br /> 2461 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct <br /> 2521 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc <br /> 2581 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac <br /> 2641 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc <br /> 2701 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga <br /> 2761 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct <br /> 2821 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct <br /> 2881 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg <br /> 2941 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag <br /> 3001 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat <br /> 3061 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt <br /> 3121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag <br /> 3181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca <br /> 3241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt <br /> 3301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc <br /> 3361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct <br /> 3421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg <br /> 3481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca <br /> 3541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact <br /> 3601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta <br /> 3661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta <br /> 3721 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct <br /> 3781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt <br /> 3841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga <br /> 3901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca <br /> 3961 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt <br /> 4021 caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc tgatttatat <br /> 4081 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat <br /> 4141 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat <br /> 4201 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc <br /> 4261 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa <br /> 4321 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg <br /> 4381 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat <br /> 4441 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt <br /> 4501 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa <br /> 4561 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt <br /> 4621 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac <br /> 4681 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag <br /> 4741 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat <br /> 4801 ttgatgctcg atgagttttt ctaagaatta attcatgagc ggatacatat ttgaatgtat <br /> 4861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat <br /> 4921 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt <br /> 4981 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg <br /> 5041 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt <br /> 5101 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc <br /> 5161 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg <br /> 5221 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa <br /> 5281 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc <br /> 5341 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg cca <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=File:Pet-33b(%2B).JPG&diff=316594 File:Pet-33b(+).JPG 2007-10-05T11:22:11Z <p>Charner: </p> <hr /> <div></div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-33b(%2B)&diff=316593 pET-33b(+) 2007-10-05T11:20:20Z <p>Charner: /* Sequence {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69054-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-33b(+) vector is derived from pET-28b(+) and carries a 15 bp sequence encoding the protein kinase A (PKA) site ArgArgAlaSerVal, located between the thrombin cleavage and Nde I sites.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat <br /> 241 gaacagatgc acgacggctg ccgcgcggca ccaggccgct gctgtgatga tgatgatgat <br /> 301 ggctgctgcc catggtatat ctccttctta aagttaaaca aaattatttc tagaggggaa <br /> 361 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatct <br /> 421 cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg <br /> 481 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga <br /> 541 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca <br /> 601 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg <br /> 661 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa <br /> 721 tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg <br /> 781 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag <br /> 841 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg <br /> 901 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc <br /> 961 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa <br /> 1021 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg <br /> 1081 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc <br /> 1141 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct <br /> 1201 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt <br /> 1261 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt <br /> 1321 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg <br /> 1381 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc <br /> 1441 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt <br /> 1501 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc <br /> 1561 gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac <br /> 1621 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa <br /> 1681 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg <br /> 1741 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct <br /> 1801 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc <br /> 1861 gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg <br /> 1921 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac <br /> 1981 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc <br /> 2041 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct <br /> 2101 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc <br /> 2161 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg <br /> 2221 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta <br /> 2281 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc <br /> 2341 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg <br /> 2401 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc <br /> 2461 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct <br /> 2521 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc <br /> 2581 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac <br /> 2641 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc <br /> 2701 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga <br /> 2761 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct <br /> 2821 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct <br /> 2881 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg <br /> 2941 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag <br /> 3001 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat <br /> 3061 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt <br /> 3121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag <br /> 3181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca <br /> 3241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt <br /> 3301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc <br /> 3361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct <br /> 3421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg <br /> 3481 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca <br /> 3541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact <br /> 3601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta <br /> 3661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta <br /> 3721 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct <br /> 3781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt <br /> 3841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga <br /> 3901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca <br /> 3961 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt <br /> 4021 caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc tgatttatat <br /> 4081 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat <br /> 4141 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat <br /> 4201 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc <br /> 4261 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa <br /> 4321 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg <br /> 4381 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat <br /> 4441 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt <br /> 4501 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa <br /> 4561 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt <br /> 4621 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac <br /> 4681 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag <br /> 4741 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat <br /> 4801 ttgatgctcg atgagttttt ctaagaatta attcatgagc ggatacatat ttgaatgtat <br /> 4861 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaaat <br /> 4921 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt <br /> 4981 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg <br /> 5041 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt <br /> 5101 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc <br /> 5161 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg <br /> 5221 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa <br /> 5281 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc <br /> 5341 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg cca <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-33b(%2B)&diff=316592 pET-33b(+) 2007-10-05T11:19:47Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69054-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-33b(+) vector is derived from pET-28b(+) and carries a 15 bp sequence encoding the protein kinase A (PKA) site ArgArgAlaSerVal, located between the thrombin cleavage and Nde I sites.<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-33b(%2B)&diff=316591 pET-33b(+) 2007-10-05T11:18:31Z <p>Charner: New page: ==At a Glance {{HelpIcon|Help:Plasmid}}== &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8--&gt; &lt;!-- *******************************************************************...</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |-<br /> !{{table heading style}} |Copy #<br /> |-<br /> !{{table heading style}} |Markers<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |-<br /> !{{table heading style}} |Comments<br /> |-<br /> |colspan='1'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.T47061d8745fa8--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-32c(%2B)_DNA&diff=316590 pET-32c(+) DNA 2007-10-05T11:17:47Z <p>Charner: /* At a Glance {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69017-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-32c(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> <br /> &lt;ref name='PMID:16908542'/&gt;<br /> &lt;ref name='PMID:15800215'/&gt;<br /> &lt;ref name='PMID:15983375'/&gt;<br /> &lt;ref name='PMID:16170303'/&gt;<br /> &lt;ref name='PMID:15961400'/&gt;<br /> &lt;ref name='PMID:14597228'/&gt;<br /> <br /> <br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tccacagata tcccatggcc ttgtcgtcgt cgtcggtacc <br /> 241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc <br /> 301 agaaccgcgt ggcaccagac cagaagaatg atgatgatga tggtgcatat ggccagaacc <br /> 361 agaaccggcc aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcacccac <br /> 421 tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc <br /> 481 atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc <br /> 541 ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca <br /> 601 ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact <br /> 661 gtcgtcagtc aggtgaataa ttttatcgct catatgtata tctccttctt aaagttaaac <br /> 721 aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt <br /> 781 aatttcgcgg gatcgagatc gatctcgatc ctctacgccg gacgcatcgt ggccggcatc <br /> 841 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat <br /> 901 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc <br /> 961 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg <br /> 1021 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag <br /> 1081 cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg <br /> 1141 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc <br /> 1201 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt <br /> 1261 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa <br /> 1321 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag <br /> 1381 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact <br /> 1441 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc <br /> 1501 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga <br /> 1561 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt <br /> 1621 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg <br /> 1681 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag <br /> 1741 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat <br /> 1801 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat <br /> 1861 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc <br /> 1921 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt <br /> 1981 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa <br /> 2041 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg <br /> 2101 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct <br /> 2161 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc <br /> 2221 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc <br /> 2281 tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac ccagtcagct <br /> 2341 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca <br /> 2401 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc <br /> 2461 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg <br /> 2521 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg <br /> 2581 ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag <br /> 2641 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa <br /> 2701 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc <br /> 2761 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct <br /> 2821 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct <br /> 2881 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac <br /> 2941 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc <br /> 3001 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat <br /> 3061 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat <br /> 3121 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat <br /> 3181 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg <br /> 3241 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg <br /> 3301 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag <br /> 3361 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga <br /> 3421 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg <br /> 3481 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc <br /> 3541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa <br /> 3601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt <br /> 3661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa <br /> 3721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt <br /> 3781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg <br /> 3841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc <br /> 3901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc <br /> 3961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta <br /> 4021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct <br /> 4081 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc <br /> 4141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa <br /> 4201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa <br /> 4261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa <br /> 4321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt <br /> 4381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac <br /> 4441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc <br /> 4501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc <br /> 4561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata <br /> 4621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc <br /> 4681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc <br /> 4741 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca <br /> 4801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa <br /> 4861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca <br /> 4921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt <br /> 4981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt <br /> 5041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg <br /> 5101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga <br /> 5161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc <br /> 5221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg <br /> 5281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag <br /> 5341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg <br /> 5401 gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta <br /> 5461 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc <br /> 5521 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg <br /> 5581 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat <br /> 5641 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc <br /> 5701 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag <br /> 5761 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg <br /> 5821 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta <br /> 5881 cagggcgcgt cccattcgcc a <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-32c(%2B)_DNA&diff=316589 pET-32c(+) DNA 2007-10-05T11:17:16Z <p>Charner: /* References */</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69017-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-32c(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> <br /> &lt;ref name='PMID:16908542'/&gt;<br /> &lt;ref name='PMID:15800215'/&gt;<br /> &lt;ref name='PMID:15983375'/&gt;<br /> &lt;ref name='PMID:16170303'/&gt;<br /> &lt;ref name='PMID:15961400'/&gt;<br /> &lt;ref name='PMID:14597228'/&gt;<br /> <br /> <br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tccacagata tcccatggcc ttgtcgtcgt cgtcggtacc <br /> 241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc <br /> 301 agaaccgcgt ggcaccagac cagaagaatg atgatgatga tggtgcatat ggccagaacc <br /> 361 agaaccggcc aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcacccac <br /> 421 tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc <br /> 481 atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc <br /> 541 ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca <br /> 601 ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact <br /> 661 gtcgtcagtc aggtgaataa ttttatcgct catatgtata tctccttctt aaagttaaac <br /> 721 aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt <br /> 781 aatttcgcgg gatcgagatc gatctcgatc ctctacgccg gacgcatcgt ggccggcatc <br /> 841 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat <br /> 901 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc <br /> 961 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg <br /> 1021 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag <br /> 1081 cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg <br /> 1141 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc <br /> 1201 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt <br /> 1261 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa <br /> 1321 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag <br /> 1381 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact <br /> 1441 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc <br /> 1501 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga <br /> 1561 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt <br /> 1621 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg <br /> 1681 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag <br /> 1741 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat <br /> 1801 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat <br /> 1861 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc <br /> 1921 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt <br /> 1981 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa <br /> 2041 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg <br /> 2101 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct <br /> 2161 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc <br /> 2221 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc <br /> 2281 tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac ccagtcagct <br /> 2341 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca <br /> 2401 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc <br /> 2461 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg <br /> 2521 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg <br /> 2581 ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag <br /> 2641 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa <br /> 2701 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc <br /> 2761 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct <br /> 2821 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct <br /> 2881 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac <br /> 2941 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc <br /> 3001 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat <br /> 3061 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat <br /> 3121 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat <br /> 3181 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg <br /> 3241 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg <br /> 3301 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag <br /> 3361 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga <br /> 3421 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg <br /> 3481 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc <br /> 3541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa <br /> 3601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt <br /> 3661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa <br /> 3721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt <br /> 3781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg <br /> 3841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc <br /> 3901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc <br /> 3961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta <br /> 4021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct <br /> 4081 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc <br /> 4141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa <br /> 4201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa <br /> 4261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa <br /> 4321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt <br /> 4381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac <br /> 4441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc <br /> 4501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc <br /> 4561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata <br /> 4621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc <br /> 4681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc <br /> 4741 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca <br /> 4801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa <br /> 4861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca <br /> 4921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt <br /> 4981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt <br /> 5041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg <br /> 5101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga <br /> 5161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc <br /> 5221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg <br /> 5281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag <br /> 5341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg <br /> 5401 gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta <br /> 5461 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc <br /> 5521 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg <br /> 5581 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat <br /> 5641 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc <br /> 5701 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag <br /> 5761 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg <br /> 5821 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta <br /> 5881 cagggcgcgt cccattcgcc a <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-32c(%2B)_DNA&diff=316588 pET-32c(+) DNA 2007-10-05T11:10:31Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69017-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> The pET-32c(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 109aa Trx•Tag™ thioredoxin protein. <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tccacagata tcccatggcc ttgtcgtcgt cgtcggtacc <br /> 241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc <br /> 301 agaaccgcgt ggcaccagac cagaagaatg atgatgatga tggtgcatat ggccagaacc <br /> 361 agaaccggcc aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcacccac <br /> 421 tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc <br /> 481 atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc <br /> 541 ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca <br /> 601 ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact <br /> 661 gtcgtcagtc aggtgaataa ttttatcgct catatgtata tctccttctt aaagttaaac <br /> 721 aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt <br /> 781 aatttcgcgg gatcgagatc gatctcgatc ctctacgccg gacgcatcgt ggccggcatc <br /> 841 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat <br /> 901 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc <br /> 961 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg <br /> 1021 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag <br /> 1081 cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg <br /> 1141 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc <br /> 1201 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt <br /> 1261 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa <br /> 1321 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag <br /> 1381 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact <br /> 1441 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc <br /> 1501 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga <br /> 1561 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt <br /> 1621 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg <br /> 1681 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag <br /> 1741 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat <br /> 1801 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat <br /> 1861 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc <br /> 1921 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt <br /> 1981 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa <br /> 2041 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg <br /> 2101 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct <br /> 2161 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc <br /> 2221 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc <br /> 2281 tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac ccagtcagct <br /> 2341 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca <br /> 2401 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc <br /> 2461 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg <br /> 2521 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg <br /> 2581 ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag <br /> 2641 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa <br /> 2701 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc <br /> 2761 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct <br /> 2821 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct <br /> 2881 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac <br /> 2941 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc <br /> 3001 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat <br /> 3061 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat <br /> 3121 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat <br /> 3181 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg <br /> 3241 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg <br /> 3301 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag <br /> 3361 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga <br /> 3421 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg <br /> 3481 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc <br /> 3541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa <br /> 3601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt <br /> 3661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa <br /> 3721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt <br /> 3781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg <br /> 3841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc <br /> 3901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc <br /> 3961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta <br /> 4021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct <br /> 4081 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc <br /> 4141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa <br /> 4201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa <br /> 4261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa <br /> 4321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt <br /> 4381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac <br /> 4441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc <br /> 4501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc <br /> 4561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata <br /> 4621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc <br /> 4681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc <br /> 4741 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca <br /> 4801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa <br /> 4861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca <br /> 4921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt <br /> 4981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt <br /> 5041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg <br /> 5101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga <br /> 5161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc <br /> 5221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg <br /> 5281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag <br /> 5341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg <br /> 5401 gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta <br /> 5461 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc <br /> 5521 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg <br /> 5581 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat <br /> 5641 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc <br /> 5701 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag <br /> 5761 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg <br /> 5821 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta <br /> 5881 cagggcgcgt cccattcgcc a <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-32c(%2B)_DNA&diff=316587 pET-32c(+) DNA 2007-10-05T11:09:35Z <p>Charner: /* Sequence {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69017-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> <br /> <br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tccacagata tcccatggcc ttgtcgtcgt cgtcggtacc <br /> 241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc <br /> 301 agaaccgcgt ggcaccagac cagaagaatg atgatgatga tggtgcatat ggccagaacc <br /> 361 agaaccggcc aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcacccac <br /> 421 tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc <br /> 481 atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc <br /> 541 ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca <br /> 601 ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact <br /> 661 gtcgtcagtc aggtgaataa ttttatcgct catatgtata tctccttctt aaagttaaac <br /> 721 aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt gagtcgtatt <br /> 781 aatttcgcgg gatcgagatc gatctcgatc ctctacgccg gacgcatcgt ggccggcatc <br /> 841 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat <br /> 901 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc <br /> 961 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg <br /> 1021 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag <br /> 1081 cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg <br /> 1141 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc <br /> 1201 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt <br /> 1261 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa <br /> 1321 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag <br /> 1381 tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact <br /> 1441 gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc <br /> 1501 ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga <br /> 1561 ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt <br /> 1621 ctctgaccag acacccatca acagtattat tttctcccat gaagacggta cgcgactggg <br /> 1681 cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag <br /> 1741 ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat <br /> 1801 tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat <br /> 1861 gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc <br /> 1921 gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt <br /> 1981 agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa <br /> 2041 acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg <br /> 2101 ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct <br /> 2161 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc <br /> 2221 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc <br /> 2281 tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac ccagtcagct <br /> 2341 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca <br /> 2401 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc <br /> 2461 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg <br /> 2521 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg <br /> 2581 ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag <br /> 2641 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa <br /> 2701 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc <br /> 2761 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct <br /> 2821 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct <br /> 2881 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac <br /> 2941 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc <br /> 3001 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat <br /> 3061 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat <br /> 3121 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat <br /> 3181 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg <br /> 3241 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg <br /> 3301 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag <br /> 3361 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga <br /> 3421 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg <br /> 3481 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc <br /> 3541 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa <br /> 3601 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt <br /> 3661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa <br /> 3721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt <br /> 3781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg <br /> 3841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc <br /> 3901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc <br /> 3961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta <br /> 4021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct <br /> 4081 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc <br /> 4141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa <br /> 4201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa <br /> 4261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa <br /> 4321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt <br /> 4381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac <br /> 4441 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc <br /> 4501 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc <br /> 4561 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata <br /> 4621 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc <br /> 4681 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc <br /> 4741 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca <br /> 4801 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa <br /> 4861 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca <br /> 4921 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt <br /> 4981 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt <br /> 5041 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg <br /> 5101 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga <br /> 5161 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc <br /> 5221 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg <br /> 5281 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag <br /> 5341 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg <br /> 5401 gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta <br /> 5461 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc <br /> 5521 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg <br /> 5581 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat <br /> 5641 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc <br /> 5701 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag <br /> 5761 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg <br /> 5821 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta <br /> 5881 cagggcgcgt cccattcgcc a <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-32c(%2B)_DNA&diff=316586 pET-32c(+) DNA 2007-10-05T11:09:00Z <p>Charner: edited by Charner via TableEdit</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/69017-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Comments<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner https://ecoliwiki.org/colipedia/index.php?title=pET-32c(%2B)_DNA&diff=316585 pET-32c(+) DNA 2007-10-05T11:07:38Z <p>Charner: New page: ==At a Glance {{HelpIcon|Help:Plasmid}}== &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt; &lt;!-- *******************************************************************...</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |-<br /> !{{table heading style}} |Copy #<br /> |-<br /> !{{table heading style}} |Markers<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |-<br /> !{{table heading style}} |Comments<br /> |-<br /> |colspan='1'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M47061af9dbcde--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSeq}}<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Charner