PMID:16418169

2007-09-25T21:47:26Z

CWaters: New PMID: Page!

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<span style = 'font-size: large'> '''Méndez-Ortiz, MM, Hyodo, M, Hayakawa, Y and Membrillo-Hernández, J''' (2006) Genome-wide transcriptional profile of Escherichia coli in response to high levels of the second messenger 3',5'-cyclic diguanylic acid.''J. Biol. Chem.'' '''281''':8090-9</span>

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|{{TableHeadingColor}} |'''Abstract''' || Cyclic diguanylic acid (c-di-GMP; cGpGp) is a global second messenger controlling motility and adhesion in bacterial cells. Intracellular concentrations of c-di-GMP depend on two opposite activities: diguanylate cyclase, recently assigned to the widespread GGDEF domain, and c-di-GMP-specific phosphodiesterase, associated with proteins harboring the EAL domain. To date, little is known about the targets of c-di-GMP in the cell or if it affects transcriptional regulation of certain genes. In order to expand our knowledge of the effect of this molecule on the bacterial metabolism, here we report on the Escherichia coli transcriptional profile under high levels of c-di-GMP. We show that an important number of genes encoding cell surface and membrane-bound proteins are altered in their transcriptional activity. On the other hand, genes encoding several transcriptional factors, such as Fur, RcsA, SoxS, and ZraR, are up-regulated, and others, such as GadE, GadX, GcvA, and MetR, are down-regulated. Transcription of motility and cell division genes were altered, and consistent with this was the physiological analysis of cells overexpressing yddV, a diguanylate cyclase; these cells displayed an abnormal cell division process when high levels of c-di-GMP were present. We also show evidence that the diguanylate cyclase gene yddV is co-transcribed with dos, a heme base oxygen sensor with c-di-GMP-specific phosphodiesterase activity. A delta dos::kan mutation rendered the cells unable to divide properly, suggesting that dos and yddV may be part of a fine-tuning mechanism for regulating the intracellular levels of c-di-GMP.
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16418169 PubMed]
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==Significance {{HelpIcon|Help:Literature}} ==
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== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
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==Annotations {{HelpIcon|Help:Literature}}==
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
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==References==
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<references/>

dosC:Expression

2007-09-25T21:47:18Z

CWaters: edited by CWaters via TableEdit

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{{Tab|Quickview|yddV}}{{Tab|Gene|yddV}}{{Tab|Gene Product(s)|yddV}}{{Tab On|Expression|yddV}}{{Tab|Evolution|yddV}}{{Tab|On One Page|yddV}}{{TabsBottom}}
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|{{Sub}}[[#Overview|Overview]]
|{{Sub}}[[#Transcription and Transcriptional Regulation|Transcription]]
|{{Sub}}[[#Translation and Regulation of Translation|Translation]]
|{{Sub}}[[#Turnover and Regulation of Turnover|Turnover]]
|{{Sub}}[[#Experimental|Experimental]]
|{{Sub}}[[#Links| Links]]
|{{Sub}}[[#References| References]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
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==Overview==
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==Transcription and Transcriptional Regulation==
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!Transcription unit(s)
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[[:Category:Txn_unit:yddV-dos|yddV-dos]]
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||{{RegulonDBfig|ECK120003373.jpg|YDDV}}
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===Notes===
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==Translation and Regulation of Translation==
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===Notes===
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==Turnover and Regulation of Turnover==
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===Notes===
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==Experimental==
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<big>'''Mutations Affecting Expression'''</big>


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!Allele Name!!Mutation!!Phenotype!!Reference
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<big>'''Expression Studies'''</big>


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!Type!!Reference!!Notes
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microarray
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[http://chase.ou.edu/macro/searchlink.php?bnum=b1490 EcoliGenExpDB:b1490] ([[EcoliGenExpDB|EcoliWiki Page]])
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Summary of data for yddV from multiple microarray studies
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microarray
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[[PMID:16418169]]<ref name=PMID:16418169/>
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yddV was overexpressed to find genes regulated by c-di-GMP in E. coli.
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<big>'''Expression Resources'''</big>


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!Resource Name!!Resource Type!!Description!!Source!!Notes
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===Notes===

==Accessions Related to ''yddV'' Expression==
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!Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G6784 EcoCyc:G6784]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB3554 EchoBASE:EB3554]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
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[http://chase.ou.edu/macro/searchlink.php?bnum=b1490 EcoliGenExpDB:b1490]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG13793 EcoGene:EG13793]
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===Notes===

==Links==
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!Name!!URL!!Comments
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==References==
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===Categories===
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[[Category:Txn_unit:yddV-dos]]

EcoliWiki - User contributions [en]

PMID:16418169

dosC:Expression