https://ecoliwiki.org/colipedia/api.php?action=feedcontributions&feedformat=atom&user=Amy EcoliWiki - User contributions [en] 2026-11-02T05:06:45Z User contributions MediaWiki 1.43.11 https://ecoliwiki.org/colipedia/index.php?title=EcoliWiki:Tracking_Plasmids&diff=328554 EcoliWiki:Tracking Plasmids 2007-12-14T01:59:28Z <p>Amy: edited by Amy via TableEdit</p> <hr /> <div>This is a page to track plasmids entered into the wiki. It is for developmental purposes only. <br /> <br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|class = 'sortable' {{Prettytable}} id='220572' <br /> |-<br /> !Plasmid Name!!Company!!Assigned To!!Date Entered!!Reviewed By!!Date Reviewed!!Date Info Added!!Date Reviewed<br /> |-<br /> |<br /> LITMUS 28i<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.03.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.03.07<br /> |<br /> 11.12.07<br /> |<br /> 11.13.07 <br /> |-<br /> |<br /> LITMUS 38i<br /> |<br /> NEB<br /> |<br /> Miriam Garcia<br /> |<br /> 08.31.2007<br /> |<br /> <br /> |<br /> <br /> |<br /> 10.31.07<br /> |<br /> <br /> |-<br /> |<br /> pGPS1.1<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 08.31.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.04.07<br /> |<br /> Miriam Garcia 11.16.07<br /> |<br /> 11.16.07 <br /> |-<br /> |<br /> pGPS2.1<br /> |<br /> NEB<br /> |<br /> Matthew Lakich<br /> |<br /> 09.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.06.07<br /> |<br /> 12.04.07<br /> |<br /> <br /> |-<br /> |<br /> pGPS3<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGPS4<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 09.03.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGPS5<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.03.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.04.07<br /> |<br /> 11.12.07<br /> |<br /> 11.13.07 <br /> |-<br /> |<br /> pMAL-c4E<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> <br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pMAL-c4G<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07<br /> |<br /> 11.12.07<br /> |<br /> 11.13.07 <br /> |-<br /> |<br /> pMAL-c4X<br /> |<br /> NEB<br /> |<br /> Matthew Lakich<br /> |<br /> 9.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pMAL-p4E<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia 11.16.07<br /> |<br /> 11.16.07 <br /> |-<br /> |<br /> pMAL-p4G<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia 11.16.07<br /> |<br /> 11.16.07 <br /> |-<br /> |<br /> pMAL-p4X<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.07.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia 11.16.07<br /> |<br /> 11.16.07 <br /> |-<br /> |<br /> pMAL-pIII<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia 11.16.07<br /> |<br /> 11.16.07 <br /> |-<br /> |<br /> pNEB193<br /> |<br /> NEB<br /> |<br /> CharnerWilliams<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pNEB206A<br /> |<br /> NEB<br /> |<br /> Charner Williams<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia 09.19.07<br /> |<br /> 09.19.07 <br /> |-<br /> |<br /> pTWIN1<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTXB1<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTXB3<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTYB1<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTYB11<br /> |<br /> NEB<br /> |<br /> Amy Rabb<br /> |<br /> 9.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pUC19<br /> |<br /> NEB<br /> |<br /> <br /> |<br /> <br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pNEBR-R1<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07<br /> |<br /> 11.18.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pNEBR-X1<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07<br /> |<br /> 11.18.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pNEBR-X1GLuc<br /> |<br /> NEB<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.08.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07<br /> |<br /> 11.18.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pNEBR-X1Hygro<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.10.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pKLAC1 <br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pKLAC1-malE <br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCMV-GLuc <br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGLuc-Basic Vector<br /> |<br /> NEB<br /> |<br /> Grant Richardson<br /> |<br /> 09.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.12.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBEc-Q<br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBEc-SBP <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBEc-SBP-Q<br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBEc-SBP-SET1 <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07<br /> |<br /> 12.02.07<br /> |<br /> 12.03.07 <br /> |-<br /> |<br /> pBEc-SBP-SET1-Q<br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07<br /> |<br /> 12.02.07<br /> |<br /> 12.03.07 <br /> |-<br /> |<br /> pBEc-SBP-SET2 <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07<br /> |<br /> 12.02.07<br /> |<br /> 12.03.07 <br /> |-<br /> |<br /> pBEc-SBP-SET2-Q<br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.13.07<br /> |<br /> 12.02.07<br /> |<br /> 12.03.07 <br /> |-<br /> |<br /> pBEc-SBP-SET3<br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07<br /> |<br /> 12.02.07<br /> |<br /> 12.03.07 <br /> |-<br /> |<br /> pBEc-SBP-SET3-Q<br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07<br /> |<br /> 12.02.07<br /> |<br /> 12.03.07 <br /> |-<br /> |<br /> pBEc-SET1 <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBEc-SET2 <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBEc-SET3 <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBEn-SBP-SET1a <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBEn-SBP-SET1b <br /> |<br /> Stratagene<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBEn-SBP-SET1c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.13.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SBP-SET2a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SBP-SET2b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SBP-SET2c<br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SBP-SET3a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SBP-SET3b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SBP-SET3c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SBPa <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBEn-SBPb <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBEn-SBPc <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.14.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBEn-SET1a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.24.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SET1b<br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SET1c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SET2a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SET2b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SET2c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SET3a <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SET3b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBEn-SET3c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pCAL-c <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pCAL-kc <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pCAL-n <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia 11.19.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pCAL-n-EK <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCAL-n-FLAG <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-11a <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-11b <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-11c <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-11d <br /> |<br /> Stratagene<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-3a <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.17.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-3b <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.17.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-3c <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.17.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-3d <br /> |<br /> Stratagene<br /> |<br /> Grant Richardson<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.17.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET100/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Miriam Garcia<br /> |<br /> 09.10.07<br /> |<br /> <br /> |<br /> <br /> |<br /> 11.01.07<br /> |<br /> <br /> |-<br /> |<br /> pET-14b<br /> |<br /> Novagen<br /> |<br /> Miriam Garcia<br /> |<br /> 09.19.07<br /> |<br /> <br /> |<br /> <br /> |<br /> 11.07.07<br /> |<br /> <br /> |-<br /> |<br /> pET-15b <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 9.20.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.21.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-16b<br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 9.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-17b <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 9.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-19b <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 9.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.23.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-20b(+) <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-21(+) <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-21a(+) <br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.2.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-21b(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.24.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-21c(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.24.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.25.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-21d(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-22b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-23(+)<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-23a(+) <br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-23b(+)<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-23c(+) <br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-23d(+) <br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia <br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-24(+) <br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia <br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-24a(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-24b(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-24c(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-24d(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.25.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.26.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-25b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 9.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-26b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 9.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-27b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 9.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-28a(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-28b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-28c(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-29a(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-29b(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-29c(+) <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-30a(+) <br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-30b(+)<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-30c(+) <br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-31b(+) DNA<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-32a(+) DNA<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-32b(+) DNA<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-32c(+) DNA<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-33b(+)<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-39b(+)<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-40b(+) <br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-41a(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-41b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-41c(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-42a(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-42b(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-42c(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.1.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.01.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-43.1a(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-43.1b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-43.1c(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-44a(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-44b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-44c(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-45b(+)<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-47b(+) <br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-48b(+)<br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.3.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-49b(+)<br /> |<br /> Novagen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.3.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-50b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-51b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-52b(+)<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-9a <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-9b<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-9c <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-9d<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 09.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.28.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pETcoco-1 <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pETcoco-2<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pLacI <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pLysE <br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pLysS<br /> |<br /> Novagen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET100/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.3.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET101/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET101/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET102/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET102/D-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET151/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET151/D-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.02.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.02.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET160-GW/CAT <br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET160/GW/D-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET161-GW/CAT <br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET161/GW/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET200/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07<br /> |<br /> <br /> |<br /> <br /> |<br /> 11.07.07<br /> |<br /> <br /> |-<br /> |<br /> pET200/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-DEST42<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pDEST15 <br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pDEST14 <br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.05.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pDEST17 <br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.4.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pDEST24<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pRSET T7<br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pRSET-E <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCRT7-E <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD102/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD102/D-TOPO<br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD202/D/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD202/D-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/Thio <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/Thio-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD-TOPO/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD-TOPO/LacZ/V5-His <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD-DEST49 <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/His/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.04.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.04.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/His A<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.5.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/His B<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.5.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/His C<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.5.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/myc-His A<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.5.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.08.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/myc-His B<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.12.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/myc-His C<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.12.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/Myc-His/lacZ<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.15.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/gIII Calmodulin<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.15.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/gIII A<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.15.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/gIII B<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.15.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/gIII C<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.15.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pBAD/Thio-E<br /> |<br /> Invitrogen<br /> |<br /> Matthew Lakich<br /> |<br /> 10.15.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pThioHis A<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pThioHis B<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pThioHis C<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis-TOPO/lacZ<br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2-TOPO/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis/CAT<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis A<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis B<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis C<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2/LacZ<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2 A<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2 B<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pTrcHis2 C<br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCX <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pCX-TOPO <br /> |<br /> Invitrogen<br /> |<br /> Grant Richardson<br /> |<br /> 10.09.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.10.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pET-30 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.26.07<br /> |<br /> 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-32 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.26.07<br /> |<br /> 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-41 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> Miriam Garcia 11.26.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.26.07<br /> |<br /> 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-43.1 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Charner Williams<br /> |<br /> Miriam Garcia 11.26.07 <br /> |<br /> Miriam Garcia<br /> |<br /> 11.26.07<br /> |<br /> 11.26.07<br /> |<br /> 11.26.07 <br /> |-<br /> |<br /> pET-44 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 11.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.13.07<br /> |<br /> 11.12.07<br /> |<br /> 11.13.07 <br /> |-<br /> |<br /> pET-46 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 11.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.13.07<br /> |<br /> 11.12.07<br /> |<br /> 11.13.07 <br /> |-<br /> |<br /> pET-51 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 11.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.13.07<br /> |<br /> 11.12.07<br /> |<br /> 11.13.07 <br /> |-<br /> |<br /> pCDF-2 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 11.17.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.19.07<br /> |<br /> 11.17.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pRSF-2 Ek/LIC<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 11.17.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.19.07<br /> |<br /> 11.17.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBAC-2cp<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 11.17.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.19.07<br /> |<br /> 11.17.07<br /> |<br /> 11.19.07 <br /> |-<br /> |<br /> pBACgus-2cp<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 12.13.07<br /> |<br /> <br /> |<br /> <br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pET-30 Xa/LIC<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 12.13.07<br /> |<br /> <br /> |<br /> <br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pET-32 Xa/LIC<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 12.13.07<br /> |<br /> <br /> |<br /> <br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pET-52 3c/LIC<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 12.13.07<br /> |<br /> <br /> |<br /> <br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pETDuet-1<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 12.13.07<br /> |<br /> <br /> |<br /> <br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pACYCDuet-1<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 12.13.07<br /> |<br /> <br /> |<br /> <br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pCOLADuet-1<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 11.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.28.07<br /> |<br /> 11.27.07<br /> |<br /> 11.28.07 <br /> |-<br /> |<br /> pCDFDuet-1<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 11.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.29.07<br /> |<br /> 11.27.07<br /> |<br /> 11.29.07 <br /> |-<br /> |<br /> pRSFDuet-1<br /> |<br /> Novagen<br /> |<br /> Stephanie Michinock<br /> |<br /> 11.27.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.29.07<br /> |<br /> 11.27.07<br /> |<br /> 11.29.07 <br /> |-<br /> |<br /> pETBlue-1<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 12/12/07<br /> |<br /> <br /> |<br /> <br /> |<br /> 12/12/07<br /> |<br /> <br /> |-<br /> |<br /> pETBlue-2<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 12/12/07<br /> |<br /> <br /> |<br /> <br /> |<br /> 12/12/07<br /> |<br /> <br /> |-<br /> |<br /> pBiEx-1<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 12/12/07<br /> |<br /> <br /> |<br /> <br /> |<br /> 12/12/07<br /> |<br /> <br /> |-<br /> |<br /> pBiEx-2<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 12/12/07<br /> |<br /> <br /> |<br /> <br /> |<br /> 12/12/07<br /> |<br /> <br /> |-<br /> |<br /> pBiEx-3<br /> |<br /> Novagen<br /> |<br /> Amy Rabb<br /> |<br /> 12/12/07<br /> |<br /> <br /> |<br /> <br /> |<br /> 12/12/07<br /> |<br /> <br /> |-<br /> |<br /> pF1A T7<br /> |<br /> Promega<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07<br /> |<br /> <br /> |<br /> <br /> |<br /> 11.07.07<br /> |<br /> <br /> |-<br /> |<br /> pF1K T7<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.11.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN2A (GST)<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.11.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN2K (GST)<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.11.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF3A WG (BYDV)<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.11.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF3K WG (BYDV)<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.11.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF4A CMV<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.11.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF4K CMV<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.11.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF5A CMV-neo<br /> |<br /> Promega<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF5K CMV-neo<br /> |<br /> Promega<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07n <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN6A (HQ)<br /> |<br /> Promega<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN6K (HQ)<br /> |<br /> Promega<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFC7A (HQ)<br /> |<br /> Promega<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFC7A (HQ)<br /> |<br /> Promega<br /> |<br /> Stephanie Michinock<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFC8A (HaloTag)<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.22.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFC8K (HaloTag)<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.22.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF9A CMV hRluc-neo<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.22.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN10A (ACT)<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.22.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pFN11A (BIND)<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.22.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF12A RM<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.22.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pF12K RM<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.22.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Xa-1 <br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.22.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pALTER-EX1 <br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.22.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pALTER-EX2<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.21.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.22.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pET-5a<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pET-5b<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pET-5c<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.15.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pGEMEX-1 <br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.22.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pGEMEX-2<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.22.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> PinPoint Control <br /> |<br /> Promega<br /> |<br /> Matthew Lakich<br /> |<br /> 10.28.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Xa Control <br /> |<br /> Promega<br /> |<br /> Matthew Lakich<br /> |<br /> 10.31.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Xa-2 <br /> |<br /> Promega<br /> |<br /> Matthew Lakich<br /> |<br /> 10.31.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> PinPoint Xa-3 <br /> |<br /> Promega<br /> |<br /> Matthew Lakich<br /> |<br /> 10.31.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-3Z<br /> |<br /> Promega<br /> |<br /> Matthew Lakich<br /> |<br /> 10.31.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-3Zf(+)<br /> |<br /> Promega<br /> |<br /> Matthew Lakich<br /> |<br /> 10.31.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.05.07<br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-3Zf(-)<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.23.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.24.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-4Z <br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.23.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.24.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-5Zf(+) <br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.23.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.24.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-5Zf(-)<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.17.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-7Zf(+) <br /> |<br /> Promega<br /> |<br /> Grant Richadson<br /> |<br /> 10.17.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-7Zf(-)<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.17.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-9Zf(-)<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.17.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-11Zf(+)<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.17.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-11Zf(-) <br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.17.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGEM-13Zf(+)<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.17.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.18.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL2-Basic <br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.19.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL2-Control <br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.19.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL2-Enhancer<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.19.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pGL2-Promoter<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.19.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pG5luc<br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.18.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.19.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP64 Poly(A) <br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.23.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.24.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP72 <br /> |<br /> Promega<br /> |<br /> Grant Richardson<br /> |<br /> 10.23.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.24.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pSP73 <br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.26.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.29.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pGEM-1<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.26.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.07.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pGEM-2 <br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.26.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.29.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pGEM-3 <br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.26.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.29.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pGEM-4 Vector <br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.26.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.29.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pGEM-15Zf(-)<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.26.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.07.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pSP64 <br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.26.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.29.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pSP65<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.26.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.29.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pSP70<br /> |<br /> Promega<br /> |<br /> Amy Rabb<br /> |<br /> 10.26.07<br /> |<br /> Miriam Garcia<br /> |<br /> 10.29.07<br /> |<br /> 12.13.07<br /> |<br /> <br /> |-<br /> |<br /> pBEn-SBP-SET3b <br /> |<br /> Stratagene<br /> |<br /> Charner Williams<br /> |<br /> 09.14.07<br /> |<br /> Miriam Garcia<br /> |<br /> 09.27.07 <br /> |<br /> <br /> |<br /> <br /> |-<br /> |<br /> pRS414<br /> |<br /> Simons Lab <br /> |<br /> Miriam Garcia<br /> |<br /> 10.25.07<br /> |<br /> <br /> |<br /> <br /> |<br /> 10.26.07<br /> |<br /> 11.14.07 <br /> |-<br /> |<br /> pRS415<br /> |<br /> Simons Lab<br /> |<br /> Amy Rabb<br /> |<br /> 11.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.13.07<br /> |<br /> 11.11.07<br /> |<br /> 11.14.07 <br /> |-<br /> |<br /> pRS1274<br /> |<br /> Simons Lab<br /> |<br /> Amy Rabb<br /> |<br /> 11.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07 <br /> |-<br /> |<br /> pRS591<br /> |<br /> Simons Lab<br /> |<br /> Amy Rabb<br /> |<br /> 11.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07 <br /> |-<br /> |<br /> pRS552<br /> |<br /> Simons Lab<br /> |<br /> Amy Rabb<br /> |<br /> 11.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07 <br /> |-<br /> |<br /> pRS551<br /> |<br /> Simons Lab<br /> |<br /> Amy Rabb<br /> |<br /> 11.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07 <br /> |-<br /> |<br /> pRS577<br /> |<br /> Simons Lab<br /> |<br /> Amy Rabb<br /> |<br /> 11.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07 <br /> |-<br /> |<br /> pRS550<br /> |<br /> Simons Lab<br /> |<br /> Amy Rabb<br /> |<br /> 11.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07 <br /> |-<br /> |<br /> pRS308<br /> |<br /> Simons Lab<br /> |<br /> Amy Rabb<br /> |<br /> 11.12.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07 <br /> |-<br /> |<br /> pRS475<br /> |<br /> Simons Lab<br /> |<br /> Amy Rabb<br /> |<br /> 11.11.07<br /> |<br /> Miriam Garcia<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07<br /> |<br /> 11.14.07 <br /> |-class='sortbottom'<br /> |[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table] || || || || || || ||<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.G46d7311d76129--&gt;&lt;/protect&gt;</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pMAL-c4E&diff=328553 pMAL-c4E 2007-12-13T22:59:18Z <p>Amy: /* References */</p> <hr /> <div>==At a Glance==<br /> [[Image:PMAL-c4E.jpg|thumb|600px|right||pMAL-c4E In red is the '''bla''' gene, with origins of replication in green, MCS in cyan, and promoters in magenta]]<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.I46dc4df6df37b--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> M13, pMB1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/pmalc4e.txt NEB]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> lacIq Promoter&lt;br&gt;<br /> Ptac Promoter&lt;br&gt;<br /> MCS<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.I46dc4df6df37b&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.I46dc4df6df37b--&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> <br /> ==History ==<br /> The maltose-binding protein was engineered for tighter binding to amylose resin than pMAL-c2.<br /> <br /> ==Sources==<br /> [http://www.neb.com/nebecomm/products/productN8105.asp New England Biolabs]<br /> <br /> ==References==<br /> &lt;ref name='PMID:3073105'/&gt;<br /> &lt;ref name='PMID:3323806'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence==<br /> 1 CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC <br /> 51 CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA <br /> 101 CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG <br /> 151 TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG <br /> 201 GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC <br /> 251 GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC <br /> 301 ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG <br /> 351 GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG <br /> 401 TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA <br /> 451 TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC <br /> 501 ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA <br /> 551 CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC <br /> 601 TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT <br /> 651 TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG <br /> 701 CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT <br /> 751 CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT <br /> 801 GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT <br /> 851 TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG <br /> 901 ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA <br /> 951 CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT <br /> 1001 CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG <br /> 1051 TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC <br /> 1101 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA <br /> 1151 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG <br /> 1201 GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT <br /> 1251 GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC <br /> 1301 GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT <br /> 1351 AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG <br /> 1401 AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG <br /> 1451 GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA <br /> 1501 GCACTTCACC AACAAGGACC ATAGCATATG AAAATCGAAG AAGGTAAACT <br /> 1551 GGTAATCTGG ATTAACGGCG ATAAAGGCTA TAACGGTCTC GCTGAAGTCG <br /> 1601 GTAAGAAATT CGAGAAAGAT ACCGGAATTA AAGTCACCGT TGAGCATCCG <br /> 1651 GATAAACTGG AAGAGAAATT CCCACAGGTT GCGGCAACTG GCGATGGCCC <br /> 1701 TGACATTATC TTCTGGGCAC ACGACCGCTT TGGTGGCTAC GCTCAATCTG <br /> 1751 GCCTGTTGGC TGAAATCACC CCGGACAAAG CGTTCCAGGA CAAGCTGTAT <br /> 1801 CCGTTTACCT GGGATGCCGT ACGTTACAAC GGCAAGCTGA TTGCTTACCC <br /> 1851 GATCGCTGTT GAAGCGTTAT CGCTGATTTA TAACAAAGAT CTGCTGCCGA <br /> 1901 ACCCGCCAAA AACCTGGGAA GAGATCCCGG CGCTGGATAA AGAACTGAAA <br /> 1951 GCGAAAGGTA AGAGCGCGCT GATGTTCAAC CTGCAAGAAC CGTACTTCAC <br /> 2001 CTGGCCGCTG ATTGCTGCTG ACGGGGGTTA TGCGTTCAAG TATGAAAACG <br /> 2051 GCAAGTACGA CATTAAAGAC GTGGGCGTGG ATAACGCTGG CGCGAAAGCG <br /> 2101 GGTCTGACCT TCCTGGTTGA CCTGATTAAA AACAAACACA TGAATGCAGA <br /> 2151 CACCGATTAC TCCATCGCAG AAGCTGCCTT TAATAAAGGC GAAACAGCGA <br /> 2201 TGACCATCAA CGGCCCGTGG GCATGGTCCA ACATCGACAC CAGCAAAGTG <br /> 2251 AATTATGGTG TAACGGTACT GCCGACCTTC AAGGGTCAAC CATCCAAACC <br /> 2301 GTTCGTTGGC GTGCTGAGCG CAGGTATTAA CGCCGCCAGT CCGAACAAAG <br /> 2351 AGCTGGCAAA AGAGTTCCTC GAAAACTATC TGCTGACTGA TGAAGGTCTG <br /> 2401 GAAGCGGTTA ATAAAGACAA ACCGCTGGGT GCCGTAGCGC TGAAGTCTTA <br /> 2451 CGAGGAAGAG TTGGTGAAAG ATCCGCGGAT TGCCGCCACT ATGGAAAACG <br /> 2501 CCCAGAAAGG TGAAATCATG CCGAACATCC CGCAGATGTC CGCTTTCTGG <br /> 2551 TATGCCGTGC GTACTGCGGT GATCAACGCC GCCAGCGGTC GTCAGACTGT <br /> 2601 CGATGAAGCC CTGAAAGACG CGCAGACTAA TTCGAGCTCG AACAACAACA <br /> 2651 ACAATAACAA TAACAACAAC CTCGGGGATG ACGATGACAA GGTACCGGAA <br /> 2701 TTCGGATCCT CTAGAGTCGA CCTGCAGGCA AGCTTGGCAC TGGCCGTCGT <br /> 2751 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC <br /> 2801 TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC <br /> 2851 ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCAGCT <br /> 2901 TGGCTGTTTT GGCGGATGAG ATAAGATTTT CAGCCTGATA CAGATTAAAT <br /> 2951 CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG CGGCAGTAGC <br /> 3001 GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG <br /> 3051 CGCCGATGGT AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG <br /> 3101 CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT <br /> 3151 CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG <br /> 3201 CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC <br /> 3251 CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA <br /> 3301 TGGCCTTTTT GCGTTTCTAC AAACTCTTTT GTTTATTTTT CTAAATACAT <br /> 3351 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA <br /> 3401 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA <br /> 3451 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG <br /> 3501 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA <br /> 3551 CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG <br /> 3601 AAGAACGTTT CCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG <br /> 3651 GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA <br /> 3701 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC <br /> 3751 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG <br /> 3801 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA <br /> 3851 GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG <br /> 3901 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC <br /> 3951 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG <br /> 4001 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG <br /> 4051 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG <br /> 4101 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT <br /> 4151 TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA <br /> 4201 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG <br /> 4251 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC <br /> 4301 ATATATACTT TAGATTGATT TACCCCGGTT GATAATCAGA AAAGCCCCAA <br /> 4351 AAACAGGAAG ATTGTATAAG CAAATATTTA AATTGTAAAC GTTAATATTT <br /> 4401 TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA <br /> 4451 TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT <br /> 4501 AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG <br /> 4551 TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA <br /> 4601 CTACGTGAAC CATCACCCAA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA <br /> 4651 AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG <br /> 4701 GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG <br /> 4751 GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC <br /> 4801 ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTAAAAG GATCTAGGTG <br /> 4851 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC <br /> 4901 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG <br /> 4951 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG <br /> 5001 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC <br /> 5051 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG <br /> 5101 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA <br /> 5151 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA <br /> 5201 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC <br /> 5251 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA <br /> 5301 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC <br /> 5351 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG <br /> 5401 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT <br /> 5451 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT <br /> 5501 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG <br /> 5551 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT <br /> 5601 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG <br /> 5651 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA <br /> 5701 GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG <br /> 5751 TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT <br /> 5801 GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG <br /> 5851 GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG <br /> 5901 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG <br /> 5951 GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG <br /> 6001 CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGCAGCGATT CACAGATGTC <br /> 6051 TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG <br /> 6101 TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG <br /> 6151 GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT <br /> 6201 ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC <br /> 6251 ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG <br /> 6301 CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA <br /> 6351 TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA <br /> 6401 TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC <br /> 6451 GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT <br /> 6501 TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT <br /> 6551 GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG <br /> 6601 AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAAATT<br /> __NOTOC__<br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:NEB Plasmids]]</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pMAL-c4E&diff=328552 pMAL-c4E 2007-12-13T22:58:14Z <p>Amy: /* References */</p> <hr /> <div>==At a Glance==<br /> [[Image:PMAL-c4E.jpg|thumb|600px|right||pMAL-c4E In red is the '''bla''' gene, with origins of replication in green, MCS in cyan, and promoters in magenta]]<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.I46dc4df6df37b--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> M13, pMB1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/pmalc4e.txt NEB]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> lacIq Promoter&lt;br&gt;<br /> Ptac Promoter&lt;br&gt;<br /> MCS<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.I46dc4df6df37b&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.I46dc4df6df37b--&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> <br /> ==History ==<br /> The maltose-binding protein was engineered for tighter binding to amylose resin than pMAL-c2.<br /> <br /> ==Sources==<br /> [http://www.neb.com/nebecomm/products/productN8105.asp New England Biolabs]<br /> <br /> ==References==<br /> &lt;ref name='PMID:3073105'/&gt;<br /> &lt;ref name='PMID:3323806'/&gt;<br /> <br /> ==Sequence==<br /> 1 CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC <br /> 51 CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA <br /> 101 CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG <br /> 151 TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG <br /> 201 GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC <br /> 251 GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC <br /> 301 ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG <br /> 351 GGTGCCAGCG TGGTGGTGTC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG <br /> 401 TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT GGGCTGATCA <br /> 451 TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC <br /> 501 ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA <br /> 551 CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC <br /> 601 TGGTCGCATT GGGTCACCAG CAAATCGCGC TGTTAGCGGG CCCATTAAGT <br /> 651 TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG <br /> 701 CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG AGTGCCATGT <br /> 751 CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT <br /> 801 GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT <br /> 851 TACCGAGTCC GGGCTGCGCG TTGGTGCGGA TATCTCGGTA GTGGGATACG <br /> 901 ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA <br /> 951 CAGGATTTTC GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT <br /> 1001 CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC GTCTCACTGG <br /> 1051 TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC <br /> 1101 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA <br /> 1151 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG <br /> 1201 GCACAATTCT CATGTTTGAC AGCTTATCAT CGACTGCACG GTGCACCAAT <br /> 1251 GCTTCTGGCG TCAGGCAGCC ATCGGAAGCT GTGGTATGGC TGTGCAGGTC <br /> 1301 GTAAATCACT GCATAATTCG TGTCGCTCAA GGCGCACTCC CGTTCTGGAT <br /> 1351 AATGTTTTTT GCGCCGACAT CATAACGGTT CTGGCAAATA TTCTGAAATG <br /> 1401 AGCTGTTGAC AATTAATCAT CGGCTCGTAT AATGTGTGGA ATTGTGAGCG <br /> 1451 GATAACAATT TCACACAGGA AACAGCCAGT CCGTTTAGGT GTTTTCACGA <br /> 1501 GCACTTCACC AACAAGGACC ATAGCATATG AAAATCGAAG AAGGTAAACT <br /> 1551 GGTAATCTGG ATTAACGGCG ATAAAGGCTA TAACGGTCTC GCTGAAGTCG <br /> 1601 GTAAGAAATT CGAGAAAGAT ACCGGAATTA AAGTCACCGT TGAGCATCCG <br /> 1651 GATAAACTGG AAGAGAAATT CCCACAGGTT GCGGCAACTG GCGATGGCCC <br /> 1701 TGACATTATC TTCTGGGCAC ACGACCGCTT TGGTGGCTAC GCTCAATCTG <br /> 1751 GCCTGTTGGC TGAAATCACC CCGGACAAAG CGTTCCAGGA CAAGCTGTAT <br /> 1801 CCGTTTACCT GGGATGCCGT ACGTTACAAC GGCAAGCTGA TTGCTTACCC <br /> 1851 GATCGCTGTT GAAGCGTTAT CGCTGATTTA TAACAAAGAT CTGCTGCCGA <br /> 1901 ACCCGCCAAA AACCTGGGAA GAGATCCCGG CGCTGGATAA AGAACTGAAA <br /> 1951 GCGAAAGGTA AGAGCGCGCT GATGTTCAAC CTGCAAGAAC CGTACTTCAC <br /> 2001 CTGGCCGCTG ATTGCTGCTG ACGGGGGTTA TGCGTTCAAG TATGAAAACG <br /> 2051 GCAAGTACGA CATTAAAGAC GTGGGCGTGG ATAACGCTGG CGCGAAAGCG <br /> 2101 GGTCTGACCT TCCTGGTTGA CCTGATTAAA AACAAACACA TGAATGCAGA <br /> 2151 CACCGATTAC TCCATCGCAG AAGCTGCCTT TAATAAAGGC GAAACAGCGA <br /> 2201 TGACCATCAA CGGCCCGTGG GCATGGTCCA ACATCGACAC CAGCAAAGTG <br /> 2251 AATTATGGTG TAACGGTACT GCCGACCTTC AAGGGTCAAC CATCCAAACC <br /> 2301 GTTCGTTGGC GTGCTGAGCG CAGGTATTAA CGCCGCCAGT CCGAACAAAG <br /> 2351 AGCTGGCAAA AGAGTTCCTC GAAAACTATC TGCTGACTGA TGAAGGTCTG <br /> 2401 GAAGCGGTTA ATAAAGACAA ACCGCTGGGT GCCGTAGCGC TGAAGTCTTA <br /> 2451 CGAGGAAGAG TTGGTGAAAG ATCCGCGGAT TGCCGCCACT ATGGAAAACG <br /> 2501 CCCAGAAAGG TGAAATCATG CCGAACATCC CGCAGATGTC CGCTTTCTGG <br /> 2551 TATGCCGTGC GTACTGCGGT GATCAACGCC GCCAGCGGTC GTCAGACTGT <br /> 2601 CGATGAAGCC CTGAAAGACG CGCAGACTAA TTCGAGCTCG AACAACAACA <br /> 2651 ACAATAACAA TAACAACAAC CTCGGGGATG ACGATGACAA GGTACCGGAA <br /> 2701 TTCGGATCCT CTAGAGTCGA CCTGCAGGCA AGCTTGGCAC TGGCCGTCGT <br /> 2751 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC <br /> 2801 TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC <br /> 2851 ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCAGCT <br /> 2901 TGGCTGTTTT GGCGGATGAG ATAAGATTTT CAGCCTGATA CAGATTAAAT <br /> 2951 CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG CGGCAGTAGC <br /> 3001 GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG <br /> 3051 CGCCGATGGT AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG <br /> 3101 CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT <br /> 3151 CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG <br /> 3201 CGGATTTGAA CGTTGCGAAG CAACGGCCCG GAGGGTGGCG GGCAGGACGC <br /> 3251 CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA <br /> 3301 TGGCCTTTTT GCGTTTCTAC AAACTCTTTT GTTTATTTTT CTAAATACAT <br /> 3351 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA <br /> 3401 ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA <br /> 3451 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG <br /> 3501 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA <br /> 3551 CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG <br /> 3601 AAGAACGTTT CCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG <br /> 3651 GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA <br /> 3701 CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC <br /> 3751 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG <br /> 3801 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA <br /> 3851 GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG <br /> 3901 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC <br /> 3951 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG <br /> 4001 CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG <br /> 4051 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG <br /> 4101 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT <br /> 4151 TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA <br /> 4201 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG <br /> 4251 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC <br /> 4301 ATATATACTT TAGATTGATT TACCCCGGTT GATAATCAGA AAAGCCCCAA <br /> 4351 AAACAGGAAG ATTGTATAAG CAAATATTTA AATTGTAAAC GTTAATATTT <br /> 4401 TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA <br /> 4451 TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT <br /> 4501 AGGGTTGAGT GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG <br /> 4551 TGGACTCCAA CGTCAAAGGG CGAAAAACCG TCTATCAGGG CGATGGCCCA <br /> 4601 CTACGTGAAC CATCACCCAA ATCAAGTTTT TTGGGGTCGA GGTGCCGTAA <br /> 4651 AGCACTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA GCTTGACGGG <br /> 4701 GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG <br /> 4751 GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC <br /> 4801 ACCCGCCGCG CTTAATGCGC CGCTACAGGG CGCGTAAAAG GATCTAGGTG <br /> 4851 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC <br /> 4901 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG <br /> 4951 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG <br /> 5001 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC <br /> 5051 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG <br /> 5101 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA <br /> 5151 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA <br /> 5201 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC <br /> 5251 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA <br /> 5301 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC <br /> 5351 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG <br /> 5401 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT <br /> 5451 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT <br /> 5501 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG <br /> 5551 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT <br /> 5601 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG <br /> 5651 AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA <br /> 5701 GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG <br /> 5751 TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT <br /> 5801 GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG <br /> 5851 GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG <br /> 5901 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG <br /> 5951 GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG <br /> 6001 CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGCAGCGATT CACAGATGTC <br /> 6051 TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG <br /> 6101 TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG <br /> 6151 GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT <br /> 6201 ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC <br /> 6251 ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG <br /> 6301 CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA <br /> 6351 TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA <br /> 6401 TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC <br /> 6451 GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT <br /> 6501 TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT <br /> 6551 GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG <br /> 6601 AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAAATT<br /> __NOTOC__<br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:NEB Plasmids]]</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pGEM-1&diff=328551 pGEM-1 2007-12-13T09:36:46Z <p>Amy: /* At a Glance */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PGEM-1.jpg|thumb|400px|right|pGEM-1, Image provided by [http://www.promega.com/figures/popup.asp?fn=0978va Promega]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.K471c1a4741bd1--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://ecoliwiki.net/colipedia/index.php/pGEM-1#Sources Promega]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 Promoter&lt;br&gt;<br /> SP6 Promoter&lt;br&gt;<br /> SP6 transcription initiation site&lt;br&gt;<br /> T7 transcription initiation site&lt;br&gt;<br /> multiple cloning sites &lt;br&gt;<br /> lac operon sequences&lt;br&gt;<br /> Hind III, Pst I, Sal I, Acc I, Sma I, Hinc II, Xba I, BamH I, Ava I, Sac I, EcoR I<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&amp;id=58196 X65300]<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.K471c1a4741bd1&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.K471c1a4741bd1--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources ==<br /> [http://www.promega.com/vectors/pgem1.txt Promega]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> &lt;pre&gt;<br /> 1 GAATACAAGC TTGGGCTGCA GGTCGACTCT AGAGGATCCC CGGGCGAGCT <br /> 51 CGAATTCCGG TCTCCCTATA GTGAGTCGTA TTAATTTCGA TAAGCCAGCT <br /> 101 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC <br /> 151 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG <br /> 201 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA <br /> 251 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG <br /> 301 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC <br /> 351 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA <br /> 401 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG <br /> 451 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT <br /> 501 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT <br /> 551 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC <br /> 601 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC <br /> 651 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG <br /> 701 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT <br /> 751 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG <br /> 801 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA <br /> 851 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA <br /> 901 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG <br /> 951 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG <br /> 1001 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT <br /> 1051 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA <br /> 1101 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC <br /> 1151 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT <br /> 1201 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG <br /> 1251 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG <br /> 1301 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC <br /> 1351 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT <br /> 1401 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC <br /> 1451 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT <br /> 1501 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT <br /> 1551 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC <br /> 1601 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT <br /> 1651 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC <br /> 1701 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT <br /> 1751 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC <br /> 1801 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT <br /> 1851 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG <br /> 1901 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC <br /> 1951 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT <br /> 2001 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG <br /> 2051 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA <br /> 2101 TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT <br /> 2151 CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT <br /> 2201 CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG <br /> 2251 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC<br /> 2301 TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TCGACGCTCT <br /> 2351 CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC <br /> 2401 GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA <br /> 2451 ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG<br /> 2501 CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC <br /> 2551 GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA<br /> 2601 TGCGTCCGGC GTAGAGGATC TGGCTAGCGA TGACCCTGCT GATTGGTTCG <br /> 2651 CTGACCATTT CCGGGGTGCG GAACGGCGTT ACCAGAAACT CAGAAGGTTC <br /> 2701 GTCCAACCAA ACCGACTCTG ACGGCAGTTT ACGAGAGAGA TGATAGGGTC <br /> 2751 TGCTTCAGTA AGCCAGATGC TACACAATTA GGCTTGTACA TATTGTCGTT <br /> 2801 AGAACGCGGC TACAATTAAT ACATAACCTT ATGTATCATA CACATACGAT <br /> 2851 TTAGGTGACA CTATA<br /> &lt;/pre&gt;<br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pGEMEX-2&diff=328550 pGEMEX-2 2007-12-13T09:34:44Z <p>Amy: /* At a Glance */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PGEMEX-2.jpg|thumb|400px|right|pGEMEX-2, Image provided by [http://www.promega.com/figures/0300/0311vbw2.gif Promega]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M4712e9fc7f80d--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&amp;id=8216954 PubMed]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> SP6 RNA polymerase transcription initiation site&lt;br&gt;<br /> Multiple cloning region&lt;br&gt;<br /> T3 RNA polymerase promoter &lt;br&gt;<br /> T3 RNA polymerase transcription initation site&lt;br&gt;<br /> T7 gene 10 start codon&lt;br&gt;<br /> T7 RNA polymerase promoter &lt;br&gt;<br /> T7 RNA polymerase transcription initiation site&lt;br&gt;<br /> Beta-lactamase (Ampr)coding region&lt;br&gt;<br /> Phage f1 region&lt;br&gt;<br /> T7 terminator region&lt;br&gt;<br /> SP6 RNA polymerase promoter&lt;br&gt;<br /> HindIII, NsiI, SphI, NotI, ApaI, XhoI, BamHI, SalI, EcoRI, SacI, SfiI<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> X65318<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.M4712e9fc7f80d&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M4712e9fc7f80d--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> The addition of two cytosine bases at positions 65 and 84 between pGEMEX-1 and pGEMEX-2.Special usage restrictions<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources==<br /> [http://www.promega.com/vectors/pgemex2.txt Promega]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 gaatactcaa gcttatgcat gcggccgcat ctagagggcc cggatccctc gaggtcgacg<br /> 61 aattccgagc tcggccgact tggcccttcc ctttagtgag ggttaataaa cttggccaat<br /> 121 tcgatccggt accagcacca ccagcggtga ggtgcggaac ttctacaacc tcaaagccca<br /> 181 taacgttgcg gatagaaccc ttctcagggt caatcagagc agcgtagttt gctgcgttcg<br /> 241 gcatcagtgc tgccagaatc gcagagtagc tatctgggtc acagtagaac acacggtcag<br /> 301 cagccggaac atagttcttg gtcagagccg cacgagcctt agtcagagcc gcaataatct<br /> 361 ccttacccag cgcaacttgg tcggtaagtg cggccttgtt ctgagtggtc tcaattacgg<br /> 421 tagcagtacc taagccctcg atgttctcat tatatttgct ttccacgtta cacagaccgg<br /> 481 caatctcagc cagaaccgca ccatccgcag ccatcgccag agattcaccc aactgagagg<br /> 541 tatactcaga gcgaacgtcg tagtggttca tcgcgtcctc aatatcataa atcagaacgt<br /> 601 cagccgtcag gagaccgtca atggtgatta ccttctcggt gtgtttgatg tccttacgtt<br /> 661 tatcgtcgag gttctcgccc ggagccagat acgctgcctg agtgcgaccc agaacaggga<br /> 721 actgagcgga tttaccgctg gagatggaac gtaccatgtg gcgagaagtg gtcacggagg<br /> 781 tacgagcgaa cgcagtcagg acttcaccgc caaatacctt caagaacaac gccagtttat<br /> 841 ctccagcagc aactacacct ttaccttggt tagtacccat ttgctgtcca ccagtcatgc<br /> 901 tagccatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt<br /> 961 gtggtctccc tatagtgagt cgtattaatt tcgcgggatc gagatctccc gggctgcatt<br /> 1021 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct<br /> 1081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa<br /> 1141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa<br /> 1201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc<br /> 1261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga<br /> 1321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc<br /> 1381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt<br /> 1441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct<br /> 1501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg<br /> 1561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta<br /> 1621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct<br /> 1681 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa<br /> 1741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt<br /> 1801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta<br /> 1861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat<br /> 1921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa<br /> 1981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct<br /> 2041 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta<br /> 2101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct<br /> 2161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg<br /> 2221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa<br /> 2281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt<br /> 2341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta<br /> 2401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca<br /> 2461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta<br /> 2521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct<br /> 2581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg<br /> 2641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac<br /> 2701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact<br /> 2761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa<br /> 2821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt<br /> 2881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat<br /> 2941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg<br /> 3001 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc<br /> 3061 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg<br /> 3121 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt<br /> 3181 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac<br /> 3241 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca<br /> 3301 tcaggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt<br /> 3361 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct<br /> 3421 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg<br /> 3481 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag<br /> 3541 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa<br /> 3601 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga<br /> 3661 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa<br /> 3721 atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg<br /> 3781 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca ggttatccgg<br /> 3841 atatagttcc tcctttcagc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt<br /> 3901 atgctagtta ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag<br /> 3961 cagccggatc aagctgggat ttaggtgaca ctata<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]][[Category:T3 Promoter]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pGEMEX-1&diff=328549 pGEMEX-1 2007-12-13T09:34:05Z <p>Amy: /* At a Glance */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PGEMEX-1.jpg|thumb|400px|right|pGEMEX-1, Image provided by [http://www.promega.com/figures/popup.asp?fn=0309va Promega]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4712e9e08d8c3--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&amp;id=8216952 PubMed]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> HindIII, NsiI, SphI, NotI, ApaI, XhoI, BamHI, SalI, EcoRI, SacI, SfiI&lt;br&gt;<br /> T7 RNA polymerase transcription initiation site&lt;br&gt;<br /> SP6 RNA polymerase transcription initiation site&lt;br&gt;<br /> T3 RNA polymerase transcriptioninitation site&lt;br&gt;<br /> T7 RNA polymerase promoter &lt;br&gt;<br /> SP6 RNA polymerase promoter&lt;br&gt;<br /> T3 RNA polymerase promoter &lt;br&gt;<br /> Multiple cloning region&lt;br&gt; <br /> T7 gene 10 start codon&lt;br&gt; <br /> T7 terminator region&lt;br&gt; <br /> Beta-lactamase (Ampr) coding region&lt;br&gt; <br /> phage f1 region <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> X65317<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.O4712e9e08d8c3&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4712e9e08d8c3--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> Special usage restrictions&lt;br&gt;<br /> The addition of two cytosine bases at positions 65 and 84 between pGEMEX-1 and pGEMEX-2.<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources ==<br /> [http://www.promega.com/vectors/pgemex1.txt Promega]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 gaatactcaa gcttatgcat gcggccgcat ctagagggcc cggatccctc gaggtcgacg<br /> 61 aattcgagct cggccgactt ggccttccct ttagtgaggg ttaataaact tggccaattc<br /> 121 gatccggtac cagcaccacc agcggtgagg tgcggaactt ctacaacctc aaagcccata<br /> 181 acgttgcgga tagaaccctt ctcagggtca atcagagcag cgtagtttgc tgcgttcggc<br /> 241 atcagtgctg ccagaatcgc agagtagcta tctgggtcac agtagaacac acggtcagca<br /> 301 gccggaacat agttcttggt cagagccgca cgagccttag tcagagccgc aataatctcc<br /> 361 ttacccagcg caacttggtc ggtaagtgcg gccttgttct gagtggtctc aattacggta<br /> 421 gcagtaccta agccctcgat gttctcatta tatttgcttt ccacgttaca cagaccggca<br /> 481 atctcagcca gaaccgcacc atccgcagcc atcgccagag attcacccaa ctgagaggta<br /> 541 tactcagagc gaacgtcgta gtggttcatc gcgtcctcaa tatcataaat cagaacgtca<br /> 601 gccgtcagga gaccgtcaat ggtgattacc ttctcggtgt gtttgatgtc cttacgttta<br /> 661 tcgtcgaggt tctcgcccgg agccagatac gctgcctgag tgcgacccag aacagggaac<br /> 721 tgagcggatt taccgctgga gatggaacgt accatgtggc gagaagtggt cacggaggta<br /> 781 cgagcgaacg cagtcaggac ttcaccgcca aataccttca agaacaacgc cagtttatct<br /> 841 ccagcagcaa ctacaccttt accttggtta gtacccattt gctgtccacc agtcatgcta<br /> 901 gccatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg aaaccgttgt<br /> 961 ggtctcccta tagtgagtcg tattaatttc gcgggatcga gatctcccgg gctgcattaa<br /> 1021 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg<br /> 1081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag<br /> 1141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa<br /> 1201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc<br /> 1261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca<br /> 1321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg<br /> 1381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct<br /> 1441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt<br /> 1501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag<br /> 1561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc<br /> 1621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac<br /> 1681 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga<br /> 1741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc<br /> 1801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg<br /> 1861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca<br /> 1921 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt<br /> 1981 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca<br /> 2041 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg<br /> 2101 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca<br /> 2161 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt<br /> 2221 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt<br /> 2281 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca<br /> 2341 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca<br /> 2401 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga<br /> 2461 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact<br /> 2521 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga<br /> 2581 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg<br /> 2641 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc<br /> 2701 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga<br /> 2761 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat<br /> 2821 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt<br /> 2881 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt<br /> 2941 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac<br /> 3001 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc<br /> 3061 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag<br /> 3121 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca<br /> 3181 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg<br /> 3241 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc<br /> 3301 aggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga<br /> 3361 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg<br /> 3421 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat<br /> 3481 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg<br /> 3541 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata<br /> 3601 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt<br /> 3661 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat<br /> 3721 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg<br /> 3781 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagg ttatccggat<br /> 3841 atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc aaggggttat<br /> 3901 gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc tttgttagca<br /> 3961 gccggatcaa gctgggattt aggtgacact ata<br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]][[Category:T3 Promoter]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-5c&diff=328548 pET-5c 2007-12-13T09:33:45Z <p>Amy: /* At a Glance */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET-5c.jpg|thumb|400px|right|pET-5c, Image provided by [http://www.promega.com/figures/0900/0958vaw2.gif, Promega]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.A4712e9bc554d9--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.promega.com/vectors/pet-5c.txt Promega]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> BamHI cloning site&lt;br&gt;<br /> Nde I cloning site&lt;br&gt;<br /> Ribosome binding site&lt;br&gt;<br /> T7 RNA polymerase promoter&lt;br&gt;<br /> T7 RNA polymerase transcription initiation site&lt;br&gt;<br /> pBR322 plasmid replication origin&lt;br&gt;<br /> Beta-lactamase gene (AmpR)&lt;br&gt;<br /> EcoRI cloning site <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.A4712e9bc554d9&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.A4712e9bc554d9--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources ==<br /> [http://www.promega.com/vectors/pet-5c.txt Promega]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 TTCGGATCCG ACCCATTTGC TGTCCACCAG TCATGCTAGC CATATGTATA<br /> 51 TCTCCTTCTT AAAGTTAAAC AAAATTATTT CTAGAGGGAA ACCGTTGTGG<br /> 101 TCTCCCTATA GTGAGTCGTA TTAATTTCGC GGGATCGAGA TCTCGATCCT<br /> 151 CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG<br /> 201 CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC<br /> 251 TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT<br /> 301 GGCCGGGGGA CTGTTGGGCG CCATCTCCTT GCATGCACCA TTCCTTGCGG<br /> 351 CGGCGGTGCT CAACGGCCTC AACCTACTAC TGGGCTGCTT CCTAATGCAG<br /> 401 GAGTCGCATA AGGGAGAGCG TCGACCGATG CCCTTGAGAG CCTTCAACCC<br /> 451 AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA<br /> 501 TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC<br /> 551 TGGGTCATTT TCGGCGAGGA CCGCTTTCGC TGGAGCGCGA CGATGATCGG<br /> 601 CCTGTCGCTT GCGGTATTCG GAATCTTGCA CGCCCTCGCT CAAGCCTTCG<br /> 651 TCACTGGTCC CGCCACCAAA CGTTTCGGCG AGAAGCAGGC CATTATCGCC<br /> 701 GGCATGGCGG CCGACGCGCT GGGCTACGTC TTGCTGGCGT TCGCGACGCG<br /> 751 AGGCTGGATG GCCTTCCCCA TTATGATTCT TCTCGCTTCC GGCGGCATCG<br /> 801 GGATGCCCGC GTTGCAGGCC ATGCTGTCCA GGCAGGTAGA TGACGACCAT<br /> 851 CAGGGACAGC TTCAAGGATC GCTCGCGGCT CTTACCAGCC TAACTTCGAT<br /> 901 CATTGGACCG CTGATCGTCA CGGCGATTTA TGCCGCCTCG GCGAGCACAT<br /> 951 GGAACGGGTT GGCATGGATT GTAGGCGCCG CCCTATACCT TGTCTGCCTC<br /> 1001 CCCGCGTTGC GTCGCGGTGC ATGGAGCCGG GCCACCTCGA CCTGAATGGA<br /> 1051 AGCCGGCGGC ACCTCGCTAA CGGATTCACC ACTCCAAGAA TTGGAGCCAA<br /> 1101 TCAATTCTTG CGGAGAACTG TGAATGCGCA AACCAACCCT TGGCAGAACA<br /> 1151 TATCCATCGC GTCCGCCATC TCCAGCAGCC GCACGCGGCG CATCTCGGGC<br /> 1201 AGCGTTGGGT CCTGGCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG<br /> 1251 GACCCGGCTA GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC<br /> 1301 CGATACGCGA GCGAACGTGA AGCGACTGCT GCTGCAAAAC GTCTGCGACC<br /> 1351 TGAGCAACAA CATGAATGGT CTTCGGTTTC CGTGTTTCGT AAAGTCTGGA<br /> 1401 AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC TGCATCGCAG<br /> 1451 GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC<br /> 1501 TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC<br /> 1551 CAGTTGTTTA CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG<br /> 1601 TAACCCGTAT CGTGAGCATC CTCTCTCGTT TCATCGGTAT CATTACCCCC<br /> 1651 ATGAACAGAA ATCCCCCTTA CACGGAGGCA TCAGTGACCA AACAGGAAAA<br /> 1701 AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA TTAACGCTTC<br /> 1751 TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA<br /> 1801 TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT<br /> 1851 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA<br /> 1901 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG<br /> 1951 TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC AGTCACGTAG<br /> 2001 CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG AGCAGATTGT<br /> 2051 ACTGAGAGTG CACCATTGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG<br /> 2101 AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC<br /> 2151 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA<br /> 2201 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC<br /> 2251 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT<br /> 2301 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA<br /> 2351 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC<br /> 2401 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG<br /> 2451 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC<br /> 2501 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG<br /> 2551 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA<br /> 2601 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA<br /> 2651 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC<br /> 2701 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT<br /> 2751 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT<br /> 2801 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT<br /> 2851 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT<br /> 2901 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA<br /> 2951 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT<br /> 3001 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT<br /> 3051 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC<br /> 3101 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC<br /> 3151 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC<br /> 3201 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC<br /> 3251 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA<br /> 3301 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA<br /> 3351 GTTTGCGCAA CGTTGTTGCC ATTGCTGCAG GCATCGTGGT GTCACGCTCG<br /> 3401 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT<br /> 3451 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC<br /> 3501 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG<br /> 3551 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC<br /> 3601 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC<br /> 3651 GACCGAGTTG CTCTTGCCCG GCGTCAACAC GGGATAATAC CGCGCCACAT<br /> 3701 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA<br /> 3751 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC<br /> 3801 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG<br /> 3851 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC<br /> 3901 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA<br /> 3951 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG<br /> 4001 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC<br /> 4051 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC<br /> 4101 GTATCACGAG GCCCTTTCGT CTTCAAGAA<br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Promega Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:T7 Promoter]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-5a&diff=328547 pET-5a 2007-12-13T09:32:29Z <p>Amy: /* At a Glance */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET-5a.jpg|thumb|400px|right|pET-5a, Image provided by [http://www.promega.com/figures/popup.asp?fn=0957va Promega]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4712e97d11c2e--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.promega.com/vectors/pet-5a.txt Promega]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> BamHI cloning site&lt;br&gt;<br /> Nde I cloning site&lt;br&gt;<br /> Ribosome binding site&lt;br&gt;<br /> T7 RNA polymerase promoter&lt;br&gt;<br /> T7 RNA polymerase transcription initiation site&lt;br&gt;<br /> pBR322 plasmid replication origin&lt;br&gt;<br /> Beta-lactamase gene (AmpR)&lt;br&gt;<br /> EcoRI cloning site <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.O4712e97d11c2e&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.O4712e97d11c2e--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> <br /> ==History ==<br /> <br /> ==Sources ==<br /> [http://www.promega.com/vectors/pet-5a.txt Promega]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 TTCGGATCCG CGACCCATTT GCTGTCCACC AGTCATGCTA GCCATATGTA<br /> 51 TATCTCCTTC TTAAAGTTAA ACAAAATTAT TTCTAGAGGG AAACCGTTGT<br /> 101 GGTCTCCCTA TAGTGAGTCG TATTAATTTC GCGGGATCGA GATCTCGATC<br /> 151 CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA CAGGTGCGGT<br /> 201 TGCTGGCGCC TATATCGCCG ACATCACCGA TGGGGAAGAT CGGGCTCGCC<br /> 251 ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT GGCAGGCCCC<br /> 301 GTGGCCGGGG GACTGTTGGG CGCCATCTCC TTGCATGCAC CATTCCTTGC<br /> 351 GGCGGCGGTG CTCAACGGCC TCAACCTACT ACTGGGCTGC TTCCTAATGC<br /> 401 AGGAGTCGCA TAAGGGAGAG CGTCGACCGA TGCCCTTGAG AGCCTTCAAC<br /> 451 CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT<br /> 501 TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG CCGGCAGCGC<br /> 551 TCTGGGTCAT TTTCGGCGAG GACCGCTTTC GCTGGAGCGC GACGATGATC<br /> 601 GGCCTGTCGC TTGCGGTATT CGGAATCTTG CACGCCCTCG CTCAAGCCTT<br /> 651 CGTCACTGGT CCCGCCACCA AACGTTTCGG CGAGAAGCAG GCCATTATCG<br /> 701 CCGGCATGGC GGCCGACGCG CTGGGCTACG TCTTGCTGGC GTTCGCGACG<br /> 751 CGAGGCTGGA TGGCCTTCCC CATTATGATT CTTCTCGCTT CCGGCGGCAT<br /> 801 CGGGATGCCC GCGTTGCAGG CCATGCTGTC CAGGCAGGTA GATGACGACC<br /> 851 ATCAGGGACA GCTTCAAGGA TCGCTCGCGG CTCTTACCAG CCTAACTTCG<br /> 901 ATCATTGGAC CGCTGATCGT CACGGCGATT TATGCCGCCT CGGCGAGCAC<br /> 951 ATGGAACGGG TTGGCATGGA TTGTAGGCGC CGCCCTATAC CTTGTCTGCC<br /> 1001 TCCCCGCGTT GCGTCGCGGT GCATGGAGCC GGGCCACCTC GACCTGAATG<br /> 1051 GAAGCCGGCG GCACCTCGCT AACGGATTCA CCACTCCAAG AATTGGAGCC<br /> 1101 AATCAATTCT TGCGGAGAAC TGTGAATGCG CAAACCAACC CTTGGCAGAA<br /> 1151 CATATCCATC GCGTCCGCCA TCTCCAGCAG CCGCACGCGG CGCATCTCGG<br /> 1201 GCAGCGTTGG GTCCTGGCCA CGGGTGCGCA TGATCGTGCT CCTGTCGTTG<br /> 1251 AGGACCCGGC TAGGCTGGCG GGGTTGCCTT ACTGGTTAGC AGAATGAATC<br /> 1301 ACCGATACGC GAGCGAACGT GAAGCGACTG CTGCTGCAAA ACGTCTGCGA<br /> 1351 CCTGAGCAAC AACATGAATG GTCTTCGGTT TCCGTGTTTC GTAAAGTCTG<br /> 1401 GAAACGCGGA AGTCAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC<br /> 1451 AGGATGCTGC TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC<br /> 1501 GCTGGCATTG ACCCTGAGTG ATTTTTCTCT GGTCCCGCCG CATCCATACC<br /> 1551 GCCAGTTGTT TACCCTCACA ACGTTCCAGT AACCGGGCAT GTTCATCATC<br /> 1601 AGTAACCCGT ATCGTGAGCA TCCTCTCTCG TTTCATCGGT ATCATTACCC<br /> 1651 CCATGAACAG AAATCCCCCT TACACGGAGG CATCAGTGAC CAAACAGGAA<br /> 1701 AAAACCGCCC TTAACATGGC CCGCTTTATC AGAAGCCAGA CATTAACGCT<br /> 1751 TCTGGAGAAA CTCAACGAGC TGGACGCGGA TGAACAGGCA GACATCTGTG<br /> 1801 AATCGCTTCA CGACCACGCT GATGAGCTTT ACCGCAGCTG CCTCGCGCGT<br /> 1851 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT<br /> 1901 CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG<br /> 1951 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT<br /> 2001 AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT<br /> 2051 GTACTGAGAG TGCACCATTG CGGTGTGAAA TACCGCACAG ATGCGTAAGG<br /> 2101 AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT<br /> 2151 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG<br /> 2201 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA<br /> 2251 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC<br /> 2301 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT<br /> 2351 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT<br /> 2401 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC<br /> 2451 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA<br /> 2501 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG<br /> 2551 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG<br /> 2601 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG<br /> 2651 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT<br /> 2701 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT<br /> 2751 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG<br /> 2801 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT<br /> 2851 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC<br /> 2901 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT<br /> 2951 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT<br /> 3001 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC<br /> 3051 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA<br /> 3101 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA<br /> 3151 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC<br /> 3201 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG<br /> 3251 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC<br /> 3301 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA<br /> 3351 TAGTTTGCGC AACGTTGTTG CCATTGCTGC AGGCATCGTG GTGTCACGCT<br /> 3401 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA<br /> 3451 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC<br /> 3501 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA<br /> 3551 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT<br /> 3601 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG<br /> 3651 GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC<br /> 3701 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA<br /> 3751 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC<br /> 3801 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG<br /> 3851 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG<br /> 3901 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG<br /> 3951 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT<br /> 4001 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA<br /> 4051 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG<br /> 4101 GCGTATCACG AGGCCCTTTC GTCTTCAAGA A<br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Promega Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:T7 Promoter]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-5b&diff=328546 pET-5b 2007-12-13T09:32:01Z <p>Amy: /* At a Glance */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET-5b.jpg|thumb|400px|right|pET-5b, Image provided by [http://www.promega.com/figures/popup.asp?fn=0956va, Promega]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.E4712e9a706ae7--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pBR322<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.promega.com/vectors/pet-5b.txt Promega]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> BamHI cloning site&lt;br&gt;<br /> Nde I cloning site&lt;br&gt;<br /> Ribosome binding site&lt;br&gt;<br /> T7 RNA polymerase promoter&lt;br&gt;<br /> T7 RNA polymerase transcription initiation site&lt;br&gt;<br /> pBR322 plasmid replication origin&lt;br&gt;<br /> Beta-lactamase gene (AmpR)&lt;br&gt;<br /> EcoRI cloning site <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.E4712e9a706ae7&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.E4712e9a706ae7--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources ==<br /> [http://www.promega.com/vectors/pet-5b.txt Promega]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 TTCGGATCCC GACCCATTTG CTGTCCACCA GTCATGCTAG CCATATGTAT<br /> 51 ATCTCCTTCT TAAAGTTAAA CAAAATTATT TCTAGAGGGA AACCGTTGTG<br /> 101 GTCTCCCTAT AGTGAGTCGT ATTAATTTCG CGGGATCGAG ATCTCGATCC<br /> 151 TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC AGGTGCGGTT<br /> 201 GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC GGGCTCGCCA<br /> 251 CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG GCAGGCCCCG<br /> 301 TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCACC ATTCCTTGCG<br /> 351 GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT TCCTAATGCA<br /> 401 GGAGTCGCAT AAGGGAGAGC GTCGACCGAT GCCCTTGAGA GCCTTCAACC<br /> 451 CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT CGCCGCACTT<br /> 501 ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCT<br /> 551 CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG ACGATGATCG<br /> 601 GCCTGTCGCT TGCGGTATTC GGAATCTTGC ACGCCCTCGC TCAAGCCTTC<br /> 651 GTCACTGGTC CCGCCACCAA ACGTTTCGGC GAGAAGCAGG CCATTATCGC<br /> 701 CGGCATGGCG GCCGACGCGC TGGGCTACGT CTTGCTGGCG TTCGCGACGC<br /> 751 GAGGCTGGAT GGCCTTCCCC ATTATGATTC TTCTCGCTTC CGGCGGCATC<br /> 801 GGGATGCCCG CGTTGCAGGC CATGCTGTCC AGGCAGGTAG ATGACGACCA<br /> 851 TCAGGGACAG CTTCAAGGAT CGCTCGCGGC TCTTACCAGC CTAACTTCGA<br /> 901 TCATTGGACC GCTGATCGTC ACGGCGATTT ATGCCGCCTC GGCGAGCACA<br /> 951 TGGAACGGGT TGGCATGGAT TGTAGGCGCC GCCCTATACC TTGTCTGCCT<br /> 1001 CCCCGCGTTG CGTCGCGGTG CATGGAGCCG GGCCACCTCG ACCTGAATGG<br /> 1051 AAGCCGGCGG CACCTCGCTA ACGGATTCAC CACTCCAAGA ATTGGAGCCA<br /> 1101 ATCAATTCTT GCGGAGAACT GTGAATGCGC AAACCAACCC TTGGCAGAAC<br /> 1151 ATATCCATCG CGTCCGCCAT CTCCAGCAGC CGCACGCGGC GCATCTCGGG<br /> 1201 CAGCGTTGGG TCCTGGCCAC GGGTGCGCAT GATCGTGCTC CTGTCGTTGA<br /> 1251 GGACCCGGCT AGGCTGGCGG GGTTGCCTTA CTGGTTAGCA GAATGAATCA<br /> 1301 CCGATACGCG AGCGAACGTG AAGCGACTGC TGCTGCAAAA CGTCTGCGAC<br /> 1351 CTGAGCAACA ACATGAATGG TCTTCGGTTT CCGTGTTTCG TAAAGTCTGG<br /> 1401 AAACGCGGAA GTCAGCGCCC TGCACCATTA TGTTCCGGAT CTGCATCGCA<br /> 1451 GGATGCTGCT GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCG<br /> 1501 CTGGCATTGA CCCTGAGTGA TTTTTCTCTG GTCCCGCCGC ATCCATACCG<br /> 1551 CCAGTTGTTT ACCCTCACAA CGTTCCAGTA ACCGGGCATG TTCATCATCA<br /> 1601 GTAACCCGTA TCGTGAGCAT CCTCTCTCGT TTCATCGGTA TCATTACCCC<br /> 1651 CATGAACAGA AATCCCCCTT ACACGGAGGC ATCAGTGACC AAACAGGAAA<br /> 1701 AAACCGCCCT TAACATGGCC CGCTTTATCA GAAGCCAGAC ATTAACGCTT<br /> 1751 CTGGAGAAAC TCAACGAGCT GGACGCGGAT GAACAGGCAG ACATCTGTGA<br /> 1801 ATCGCTTCAC GACCACGCTG ATGAGCTTTA CCGCAGCTGC CTCGCGCGTT<br /> 1851 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC<br /> 1901 ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC<br /> 1951 GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA<br /> 2001 GCGATAGCGG AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG<br /> 2051 TACTGAGAGT GCACCATTGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA<br /> 2101 GAAAATACCG CATCAGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG<br /> 2151 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT<br /> 2201 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG<br /> 2251 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG<br /> 2301 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC<br /> 2351 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC<br /> 2401 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC<br /> 2451 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG<br /> 2501 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG<br /> 2551 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT<br /> 2601 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC<br /> 2651 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA<br /> 2701 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA<br /> 2751 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG<br /> 2801 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG<br /> 2851 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT<br /> 2901 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA<br /> 2951 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT<br /> 3001 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT<br /> 3051 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT<br /> 3101 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA<br /> 3151 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG<br /> 3201 CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC<br /> 3251 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC<br /> 3301 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT<br /> 3351 AGTTTGCGCA ACGTTGTTGC CATTGCTGCA GGCATCGTGG TGTCACGCTC<br /> 3401 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG<br /> 3451 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT<br /> 3501 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT<br /> 3551 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT<br /> 3601 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG<br /> 3651 CGACCGAGTT GCTCTTGCCC GGCGTCAACA CGGGATAATA CCGCGCCACA<br /> 3701 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA<br /> 3751 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT<br /> 3801 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG<br /> 3851 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA<br /> 3901 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC<br /> 3951 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA<br /> 4001 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC<br /> 4051 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG<br /> 4101 CGTATCACGA GGCCCTTTCG TCTTCAAGAA<br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Promega Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:T7 Promoter]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=PinPoint_Xa-1&diff=328545 PinPoint Xa-1 2007-12-13T09:31:03Z <p>Amy: /* At a Glance */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PINPOINTXA-1.jpg|thumb|400px|right|PinPoint Xa-1, Image provided by [http://www.promega.com/figures/popup.asp?fn=0923va Promega]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D4712e90d49a22--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&amp;id=9055393 PubMed]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 Promoter&lt;br&gt;<br /> tac Promoter&lt;br&gt;<br /> SP6 Promoter<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&amp;id=9055393 U47626]<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.D4712e90d49a22&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D4712e90d49a22--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources ==<br /> [http://www.promega.com/vectors/pinpnt1.txt Promega]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 atgaaactga aggtaacagt caacggcact gcgtatgacg ttgacgttga cgtcgacaag<br /> 61 tcacacgaaa acccgatggg caccatcctg ttcggcggcg gcaccggcgg cgcgccggca<br /> 121 ccggcagcag gtggcgcagg cgccggtaag gccggagagg gcgagattcc cgctccgctg<br /> 181 gccggcaccg tctccaagat cctcgtgaag gagggtgaca cggtcaaggc tggtcagacc<br /> 241 gtgctcgttc tcgaggccat gaagatggag accgagatca acgctcccac cgacggcaag<br /> 301 gtcgagaagg tcctggtcaa ggagcgtgac gcggtgcagg gcggtcaggg tctcatcaag<br /> 361 atcggggatc tcgagctcat cgaaggtcgc gaagcttcag ctgggatccg gtaccgatat<br /> 421 cagatctccc ggggcggccg cgatctggtt ctatagtgtc acctaaatcg tatgtgtatg<br /> 481 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgtc catatggtgc<br /> 541 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca<br /> 601 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg<br /> 661 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga<br /> 721 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct<br /> 781 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc<br /> 841 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa<br /> 901 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt<br /> 961 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct<br /> 1021 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc<br /> 1081 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta<br /> 1141 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac<br /> 1201 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc<br /> 1261 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac<br /> 1321 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg<br /> 1381 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac<br /> 1441 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc<br /> 1501 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt<br /> 1561 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga<br /> 1621 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc<br /> 1681 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag<br /> 1741 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca<br /> 1801 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc<br /> 1861 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca<br /> 1921 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc<br /> 1981 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta<br /> 2041 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt<br /> 2101 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc<br /> 2161 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg<br /> 2221 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg<br /> 2281 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag<br /> 2341 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc<br /> 2401 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat<br /> 2461 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg<br /> 2521 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc<br /> 2581 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt<br /> 2641 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca<br /> 2701 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg<br /> 2761 attcattaat gcaggttaac ctggcttatc gaaattaata cgactcacta tagggagacc<br /> 2821 ggcctcgagc agcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat<br /> 2881 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt<br /> 2941 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat<br /> 3001 gcgtccggcg tagaggatcg atccgggctt atcgactgca cggtgcacca atgcttctgg<br /> 3061 cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt<br /> 3121 cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac atcataacgg<br /> 3181 ttctggcaaa tattctgaaa tgagctgttg acaattaatc atcggctcgt ataatgtgtg<br /> 3241 gaattgtgag cggataacaa tttcacacag gaaacagaat tcccagcttg gctgcagaac<br /> 3301 cattccattc gttgatccgg gagtaactca c<br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:tac Promoter]][[Category:SP6 Promoter]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pALTER-EX1&diff=328544 pALTER-EX1 2007-12-13T09:30:35Z <p>Amy: /* At a Glance */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PALTER-EX1.jpg|thumb|400px|right|pALTER-EX1, Image provided by [http://www.promega.com/figures/popup.asp?fn=0580va Promega]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.S4712e93300d41--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Chloramphenicol acetyltransferase, Ampicillin, Tetracycline<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.promega.com/vectors/paltex1.txt Promega]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 RNA polymerase trancription initiation site&lt;br&gt; <br /> SP6 RNA polymerase transcription initiation site&lt;br&gt;<br /> tac transcription initiation site&lt;br&gt;<br /> T7 RNA polymerase promoter&lt;br&gt;<br /> SP6 RNA polymerase promoter&lt;br&gt;<br /> tac promoter&lt;br&gt;<br /> T7 translation start codon&lt;br&gt;<br /> tac and SP6 translation start codon&lt;br&gt;<br /> multiple cloning region&lt;br&gt;<br /> lacZ start codon&lt;br&gt;<br /> lac operon sequences&lt;br&gt;<br /> lac operator&lt;br&gt;<br /> Beta-lactamase (AmpR) coding region&lt;br&gt;<br /> TetR gene coding region&lt;br&gt;<br /> Chloramphenicol acetyltransferase (CmR) coding region&lt;br&gt;<br /> phage f1 region&lt;br&gt;<br /> binding site of pUC/M13 forward sequencing primer&lt;br&gt;<br /> binding site of pUC/M13 reverse sequencing primer&lt;br&gt;<br /> Binding site of Ampicillin Repair Oligonucleotide&lt;br&gt;<br /> Binding site of Tetracylcine Repair Oligonucleotide&lt;br&gt;<br /> Binding site of Chloramphenicol Repair Oligonucleotide&lt;br&gt;<br /> EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, PstI, SphI, HindIII<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> U47102<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.S4712e93300d41&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.S4712e93300d41--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector is tetracyline resistant and ampicillin sensitive as supplied by Promega.<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources ==<br /> [http://www.promega.com/vectors/paltex1.txt Promega]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> &lt;pre&gt;<br /> 1 GGGCGAATTC GTTAACTTTA AGAAGGAGAT ATACCCATGG AGGCCTCTAG<br /> 51 ACTGCAGCGG CCGCATGCAT ATGGATCCTG TTTCCTGTGT GAAATTGTTA<br /> 101 TCCGCTCACA ATTCCACACA TTATACGAGC CGATGATTAA TTGTCAACAG<br /> 151 GGGGATGGGG AGTAAGCTTG AGTATTCTAT AGTGTCACCT AAATAGCTTG<br /> 201 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC<br /> 251 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG<br /> 301 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT<br /> 351 TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG<br /> 401 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA<br /> 451 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC<br /> 501 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA<br /> 551 GAACATGAAT TAATTCTCAT GTTTGACAGC TTATCATCGG ATAACCCCTT<br /> 601 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGTTCTCAT GTTTGACAGC<br /> 651 TTATCTTCGA TTAGCTTTAA TGCGGTAGTT TATCACAGTT AAATTGCTAA<br /> 701 CGCAGTCAGG CACCGTGTAT GAAATCTAAC AATGCGCTCA TCGTCATCCT<br /> 751 CGGCACCGTC ACCCTGGATG CTGTAGGCAT AGGCTTGGTT ATGCCGGTAC<br /> 801 TGCCGGGCCT CTTGCGGGAT ATCGTCCATT CCGACAGCAT CGCCAGTCAC<br /> 851 TATGGCGTGC TGCTAGCGCT ATATGCGTTG ATGCAATTTC TATGCGCACC<br /> 901 CGTTCTCGGA GCACTGTCCG ACCGCTTTGG CCGCCGCCCA GTCCTGCTCG<br /> 951 CTTCGCTACT TGGAGCCACT ATCGACTACG CGATCATGGC GACCACACCC<br /> 1001 GTCCTGTGGA TTCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC<br /> 1051 CACAGGTGCG GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG<br /> 1101 ATCGGGCTCG CCACTTCGGG CTCATGAGCG CTTGTTTCGG CGTGGGTATG<br /> 1151 GTGGCAGGCC CCGTGGCCGG GGGACTGTTG GGCGCCATCT CCTTACATGC<br /> 1201 ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA CTACTGGGCT<br /> 1251 GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGCCGACC CATGCCCTTG<br /> 1301 AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG GCATGACTAT<br /> 1351 CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG<br /> 1401 TGCCGGCAGC GCTCTGGGTC ATTTTCGGCG AGGACCGCTT TCGCTGGAGC<br /> 1451 GCGACGATGA TCGGCCTGTC GCTTGCGGTA TTCGGAATCT TGCACGCCCT<br /> 1501 CGCTCAAGCC TTCGTCACTG GTCCCGCCAC CAAACGTTTC GGCGAGAAGC<br /> 1551 AGGCCATTAT CGCCGGCATG GCGGCCGACG CGCTGGGCTA CGTCTTGCTG<br /> 1601 GCGTTCGCGA CGCGAGGCTG GATGGCCTTC CCCATTATGA TTCTTCTCGC<br /> 1651 TTCCGGCGGC ATCGGGATGC CCGCGTTGCA GGCCATGCTG TCCAGGCAGG<br /> 1701 TAGATGACGA CCATCAGGGA CAGCTTCAAG GATCGCTCGC GGCTCTTACC<br /> 1751 AGCCTAACTT CGATCATTGG ACCGCTGATC GTCACGGCGA TTTATGCCGC<br /> 1801 CTCGGCGAGC ACATGGAACG GGTTGGCATG GATTGTAGGC GCCGCCCTAT<br /> 1851 ACCTTGTCTG CCTCCCCGCG TTGCGTCGCG GTGCATGGAG CCGGGCCACC<br /> 1901 TCGACCTGAA TGGAAGCCGG CGGCACCTCG CTAACGGATT CACCACTCCA<br /> 1951 AGAATTGGAG CCAATCAATT CTTGCGGAGA ACTGTGAATG CGCAAACCAA<br /> 2001 CCCTTGGCAG AACATATCCA TCGCGTCCGC CATCTCCAGC AGCCGCACGC<br /> 2051 GGCGCATCTC GGGCAGCGTT GGGTCCTGGC CACGGGTGCG CATGATCGTG<br /> 2101 CTCCTGTCGT TGAGGACCCG GCTAGGCTGG CGGGGTTGCC TTACTGGTTA<br /> 2151 GCAGAATGAA TCACCGATAC GCGAGCGAAC GTGAAGCGAC TGCTGCTGCA<br /> 2201 AAACGTCTGC GACCTGAGCA ACAACATGAA TGGTCTTCGG TTTCCGTGTT<br /> 2251 TCGTAAAGTC TGGAAACGCG GAAGTCAGCG CCCTGCACCA TTATGTTCCG<br /> 2301 GATCTGCATC GCAGGATGCT GCTGGCTACC CTGTGGAACA CCTACATCTG<br /> 2351 TATTAACGAA GCGCTGGCAT TGACCCTGAG TGATTTTTCT CTGGTCCCGC<br /> 2401 CGCATCCATA CCGCCAGTTG TTTACCCTCA CAACGTTCCA GTAACCGGGC<br /> 2451 ATGTTCATCA TCAGTAACCC GTATCGTGAG CATCCTCTCT CGTTTCATCG<br /> 2501 GTATCATTAC CCCCATGAAC AGAAATCCCC CTTACACGGA GGCATCAGTG<br /> 2551 ACCAAACAGG AAAAAACCGC CCTTAACATG GCCCGCTTTA TCAGAAGCCA<br /> 2601 GACATTAACG CTTCTGGAGA AACTCAACGA GCTGGACGCG GATGAACAGG<br /> 2651 CAGACATCTG TGAATCGCTT CACGACCACG CTGATGAGCT TTACCGCAGC<br /> 2701 TGCCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT<br /> 2751 CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG<br /> 2801 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CGCAGCCATG<br /> 2851 ACCCAGTCAC GTAGCGATAG CGGAGTGTAT ACTGGCTTAA CTATGCGGCA<br /> 2901 TCAGAGCAGA TTGTACTGAG AGTGCACCCT ATGCGGTGTG AAATACCGCA<br /> 2951 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC<br /> 3001 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT<br /> 3051 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG<br /> 3101 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG<br /> 3151 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC<br /> 3201 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG<br /> 3251 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA<br /> 3301 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG<br /> 3351 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT<br /> 3401 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT<br /> 3451 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC<br /> 3501 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA<br /> 3551 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA<br /> 3601 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC<br /> 3651 GGAAAAAGCG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG<br /> 3701 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT<br /> 3751 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC<br /> 3801 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT<br /> 3851 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA<br /> 3901 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA<br /> 3951 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC<br /> 4001 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG<br /> 4051 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA<br /> 4101 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT<br /> 4151 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA<br /> 4201 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC GGCATCGTGG<br /> 4251 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA<br /> 4301 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC<br /> 4351 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC<br /> 4401 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA<br /> 4451 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA<br /> 4501 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAACA CGGGATAATA<br /> 4551 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT<br /> 4601 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT<br /> 4651 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA<br /> 4701 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA<br /> 4751 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA<br /> 4801 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG<br /> 4851 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA<br /> 4901 AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA<br /> 4951 TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG<br /> 5001 ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT<br /> 5051 CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG<br /> 5101 TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG<br /> 5151 TACTGAGAGT GCACCCTATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG<br /> 5201 AGAAAATACC GCATCAGGAA ATTGTAAGCG TTAATATTTT GTTAAAATTC<br /> 5251 GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT<br /> 5301 CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG<br /> 5351 TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC<br /> 5401 GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC<br /> 5451 ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC<br /> 5501 GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG<br /> 5551 AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC<br /> 5601 GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC<br /> 5651 TTAATGCGCC GCTACAGGGC GCGTCCATTC GCCATTCAGG CTGCGCAACT<br /> 5701 GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG<br /> 5751 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC<br /> 5801 CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGTAA TACGACTCAC<br /> 5851 TATA<br /> <br /> <br /> &lt;/pre&gt;<br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Tetracycline Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]][[Category:tac Promoter]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pALTER-EX2&diff=328543 pALTER-EX2 2007-12-13T09:30:07Z <p>Amy: /* At a Glance */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PALTER-EX2.jpg|thumb|400px|right|pALTER-EX2, Image provided by [http://www.promega.com/figures/popup.asp?fn=0581va Promega]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W4712e95000809--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> p15a<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> <br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Chloramphenicol acetyltransferase, Tetracycline, Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.promega.com/vectors/paltex2.txt Promega]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 RNA polymerase trancription initiation site&lt;br&gt; <br /> SP6 RNA polymerase transcription initiation site&lt;br&gt;<br /> tac transcription initiation site&lt;br&gt;<br /> T7 RNA polymerase promoter&lt;br&gt;<br /> SP6 RNA polymerase promoter&lt;br&gt;<br /> tac promoter&lt;br&gt;<br /> T7 translation start codon&lt;br&gt;<br /> tac and SP6 translation start codon&lt;br&gt;<br /> multiple cloning region&lt;br&gt;<br /> lacZ start codon&lt;br&gt;<br /> lac operon sequences&lt;br&gt;<br /> lac operator&lt;br&gt;<br /> Beta-lactamase (AmpR) coding region&lt;br&gt;<br /> TetR gene coding region&lt;br&gt;<br /> Chloramphenicol acetyltransferase (CmR) coding region&lt;br&gt;<br /> phage f1 region&lt;br&gt;<br /> binding site of pUC/M13 forward sequencing primer&lt;br&gt;<br /> binding site of pUC/M13 reverse sequencing primer&lt;br&gt;<br /> Binding site of Ampicillin Repair Oligonucleotide&lt;br&gt;<br /> Binding site of Tetracylcine Repair Oligonucleotide&lt;br&gt;<br /> Binding site of Chloramphenicol Repair Oligonucleotide&lt;br&gt;<br /> EcoRI, HpaI, NcoI, StuI, PstI, NotI, SphI, NsiI, NdeI, BamHI, HindIII<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> U47103<br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.W4712e95000809&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W4712e95000809--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector is tetracycline resistant and chloramphenicol sensitive as supplied by Promega<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources ==<br /> [http://www.promega.com/vectors/paltex2.txt Promega]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> &lt;pre&gt;<br /> 1 GGGCGAATTC GTTAACTTTA AGAAGGAGAT ATACCCATGG AGGCCTCTAG<br /> 51 ACTGCAGCGG CCGCATGCAT ATGGATCCTG TTTCCTGTGT GAAATTGTTA<br /> 101 TCCGCTCACA ATTCCACACA TTATACGAGC CGATGATTAA TTGTCAACAG<br /> 151 GGGGATGGGG AGTAAGCTTG AGTATTCTAT AGTGTCACCT AAATAGCTTG<br /> 201 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC<br /> 251 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG<br /> 301 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT<br /> 351 TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG<br /> 401 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA<br /> 451 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC<br /> 501 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA<br /> 551 GAACATGAAT TAATTCTCAT GTTTGACAGC TTATCATCGG ATAACCCCTT<br /> 601 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGTTCTCAT GTTTGACAGC<br /> 651 TTATCTTCGA TTAGCTTTAA TGCGGTAGTT TATCACAGTT AAATTGCTAA<br /> 701 CGCAGTCAGG CACCGTGTAT GAAATCTAAC AATGCGCTCA TCGTCATCCT<br /> 751 CGGCACCGTC ACCCTGGATG CTGTAGGCAT AGGCTTGGTT ATGCCGGTAC<br /> 801 TGCCGGGCCT CTTGCGGGAT ATCGTCCATT CCGACAGCAT CGCCAGTCAC<br /> 851 TATGGCGTGC TGCTAGCGCT ATATGCGTTG ATGCAATTTC TATGCGCACC<br /> 901 CGTTCTCGGA GCACTGTCCG ACCGCTTTGG CCGCCGCCCA GTCCTGCTCG<br /> 951 CTTCGCTACT TGGAGCCACT ATCGACTACG CGATCATGGC GACCACACCC<br /> 1001 GTCCTGTGGA TTCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC<br /> 1051 CACAGGTGCG GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG<br /> 1101 ATCGGGCTCG CCACTTCGGG CTCATGAGCG CTTGTTTCGG CGTGGGTATG<br /> 1151 GTGGCAGGCC CCGTGGCCGG GGGACTGTTG GGCGCCATCT CCTTACATGC<br /> 1201 ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA CTACTGGGCT<br /> 1251 GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGCCGACC CATGCCCTTG<br /> 1301 AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG GCATGACTAT<br /> 1351 CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG<br /> 1401 TGCCGGCAGC GCTCTGGGTC ATTTTCGGCG AGGACCGCTT TCGCTGGAGC<br /> 1451 GCGACGATGA TCGGCCTGTC GCTTGCGGTA TTCGGAATCT TGCACGCCCT<br /> 1501 CGCTCAAGCC TTCGTCACTG GTCCCGCCAC CAAACGTTTC GGCGAGAAGC<br /> 1551 AGGCCATTAT CGCCGGCATG GCGGCCGACG CGCTGGGCTA CGTCTTGCTG<br /> 1601 GCGTTCGCGA CGCGAGGCTG GATGGCCTTC CCCATTATGA TTCTTCTCGC<br /> 1651 TTCCGGCGGC ATCGGGATGC CCGCGTTGCA GGCCATGCTG TCCAGGCAGG<br /> 1701 TAGATGACGA CCATCAGGGA CAGCTTCAAG GATCGCTCGC GGCTCTTACC<br /> 1751 AGCCTAACTT CGATCATTGG ACCGCTGATC GTCACGGCGA TTTATGCCGC<br /> 1801 CTCGGCGAGC ACATGGAACG GGTTGGCATG GATTGTAGGC GCCGCCCTAT<br /> 1851 ACCTTGTCTG CCTCCCCGCG TTGCGTCGCG GTGCATGGAG CCGGGCCACC<br /> 1901 TCGACCTGAA TGGAAGCCGG CGGCACCTCG CTAACGGATT CACCACTCCA<br /> 1951 AGAATTGGAG CCAATCAATT CTTGCGGAGA ACTGTGAATG CGCAAACCAA<br /> 2001 CCCTTGGCAG AACATATCCA TCGCGTCCGC CATCTCCAGC AGCCGCACGC<br /> 2051 GGCGCATCTC GGGCAGCGTT GGGTCCTGGC CACGGGTGCG CATGATCGTG<br /> 2101 CTCCTGTCGT TGAGGACCCG GCTAGGCTGG CGGGGTTGCC TTACTGGTTA<br /> 2151 GCAGAATGAA TCACCGATAC GCGAGCGAAC GTGAAGCGAC TGCTGCTGCA<br /> 2201 AAACGTCTGC GACCTGAGCA ACAACATGAA TGGTCTTCGG TTTCCGTGTT<br /> 2251 TCGTAAAGTC TGGAAACGCG GAAGTCCCCT ACGTGCTGCT GAAGTTGCCC<br /> 2301 GCAACAGAGA GTGGAACCAA CCGGTGATAC CACGATACTA TGACTGAGAG<br /> 2351 TCAACGCCAT GAGCGGCCTC ATTTCTTATT CTGAGTTACA ACAGTCCGCA<br /> 2401 CCGCTGCCGG TAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC<br /> 2451 GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC AGGTGCCGGC<br /> 2501 AGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA<br /> 2551 AACAAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC AGGATGCTGC<br /> 2601 TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC GCTAACCGTT<br /> 2651 TTTATCAGGC TCTGGGAGGC AGAATAAATG ATCATATCGT CAATTATTAC<br /> 2701 CTCCACGGGG AGAGCCTGAG CAAACTGGCC TCAGGCATTT GAGAAGCACA<br /> 2751 CGGTCACACT GCTTCCGGTA GTCAATAAAC CGGTAAACCA GCAATAGACA<br /> 2801 TAAGCGGCTA TTTAACGACC CTGCCCTGAA CCGACGACCG GGTCGAATTT<br /> 2851 GCTTTCGAAT TTCTGCCATT CATCCGCTTA TTATCACTTA TTCAGGCGTA<br /> 2901 GCAACCAGGC GTTTAAGGGC ACCAATAACT GCCTTAAAAA AATTACGCCC<br /> 2951 CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG<br /> 3001 ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT<br /> 3051 CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATCGTG AAAACGGGGG<br /> 3101 CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC<br /> 3151 ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG<br /> 3201 GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT<br /> 3251 GTAGAAACTG CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC<br /> 3301 GTTTCAGTTT GCTCATGGAA AACGGTGTAA CAAGGGTGAA CACTATCCCA<br /> 3351 TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAACCGG ATGAGCATTC<br /> 3401 ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT<br /> 3451 TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT<br /> 3501 TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA<br /> 3551 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT<br /> 3601 TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG<br /> 3651 GTAGTGATCT TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCGA<br /> 3701 TCAACGTCTC ATTTTCGCCA AAAGTTGGCC CAGGGCTTCC CGGTATCAAC<br /> 3751 AGGGACACCA GGATTTATTT ATTCTGCGAA GTGATCTTCC GTCACAGGTA<br /> 3801 TTTATTCGGC GCAAAGTGCG TCGGGTGATG CTGCCAACTT ACTGATTTAG<br /> 3851 TGTATGATGG TGTTTTTGAG GTGCTCCAGT GGCTTCTGTT TCTATCAGCT<br /> 3901 GTCCCTCCTG TTCAGCTACT GACGGGGTGG TGCGTAACGG CAAAAGCACC<br /> 3951 GCCGGACATC AGCGCTAGCG GAGTGTATAC TGGCTTACTA TGTTGGCACT<br /> 4001 GATGAGGGTG TCAGTGAAGT GCTTCATGTG GCAGGAGAAA AAAGGCTGCA<br /> 4051 CCGGTGCGTC AGCAGAATAT GTGATACAGG ATATATTCCG CTTCCTCGCT<br /> 4101 CACTGACTCG CTACGCTCGG TCGTTCGACT GCGGCGAGCG GAAATGGCTT<br /> 4151 ACGAACGGGG CGGAGATTTC CTGGAAGATG CCAGGAAGAT ACTTAACAGG<br /> 4201 GAAGTGAGAG GGCCGCGGCA AAGCCGTTTT TCCATAGGCT CCGCCCCCCT<br /> 4251 GACAAGCATC ACGAAATCTG ACGCTCAAAT CAGTGGTGGC GAAACCCGAC<br /> 4301 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGCGGCTCC CTCGTGCGCT<br /> 4351 CTCCTGTTCC TGCCTTTCGG TTTACCGGTG TCATTCCGCT GTTATGGCCG<br /> 4401 CGTTTGTCTC ATTCCACGCC TGACACTCAG TTCCGGGTAG GCAGTTCGCT<br /> 4451 CCAAGCTGGA CTGTATGCAC GAACCCCCCG TTCAGTCCGA CCGCTGCGCC<br /> 4501 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGAAAGAC ATGCAAAAGC<br /> 4551 ACCACTGGCA GCAGCCACTG GTAATTGATT TAGAGGAGTT AGTCTTGAAG<br /> 4601 TCATGCGCCG GTTAAGGCTA AACTGAAAGG ACAAGTTTTG GTGACTGCGC<br /> 4651 TCCTCCAAGC CAGTTACCTC GGTTCAAAGA GTTGGTAGCT CAGAGAACCT<br /> 4701 TCGAAAAACC GCCCTGCAAG GCGGTTTTTT CGTTTTCAGA GCAAGAGATT<br /> 4751 ACGCGCAGAC CAAAACGATC TCAAGAAGAT CATCTTATTA ATCAGATAAA<br /> 4801 ATATTTCTAG ATTTCAGTGC AATTTATCTC TTCAAATGTA GCACCTGAAG<br /> 4851 TCAGCCCCAT ACGATATAAG TTGTAATTCT CATGTTTGAC AGCTTATCAT<br /> 4901 CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA<br /> 4951 CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA<br /> 5001 CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG<br /> 5051 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG<br /> 5101 GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTAGAGAG TGCACCCTAT<br /> 5151 GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGA<br /> 5201 AATTGTAAGC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA<br /> 5251 TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA<br /> 5301 TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA<br /> 5351 GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG<br /> 5401 TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT<br /> 5451 TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC<br /> 5501 CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG<br /> 5551 GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC<br /> 5601 ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG<br /> 5651 CGCGTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT<br /> 5701 GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA<br /> 5751 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA<br /> 5801 ACGACGGCCA GTGAATTGTA ATACGACTCA CTATA<br /> <br /> <br /> &lt;/pre&gt;<br /> [[Category:Plasmids]][[Category:Ampicillin Marker Plasmids]][[Category:Tetracycline Marker Plasmids]][[Category:Promega Plasmids]][[Category:T7 Promoter]][[Category:SP6 Promoter]][[Category:tac Promoter]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pACYCDuet-1&diff=328542 pACYCDuet-1 2007-12-13T09:10:12Z <p>Amy: /* Notes */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PACYCDUET-1.jpg|thumb|250px|right|Image of pACYCDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> P15A<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> 10-12<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> chloramphenicol<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71147-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;His•Tag coding sequence&lt;br&gt;Multiple cloning sites<br /> (Nco I–Afl II)&lt;br&gt;S•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;P15A origin&lt;br&gt;cat (CmR) coding sequence&lt;br&gt;lacI coding sequence <br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be transformed into the same cell with pETDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes.&lt;ref&gt;Novy, R., et all. 2002. ''inNovations '''''15''',2.&lt;/ref&gt;&lt;ref&gt;Held, D., et al. 2003. ''inNovations'' '''18''', 4.&lt;/ref&gt;.<br /> <br /> ==History ==<br /> pACYCDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:17269935'/&gt;<br /> &lt;ref name='PMID:17804794'/&gt;<br /> &lt;ref name='PMID:16369554'/&gt;<br /> &lt;ref name='PMID:17060320'/&gt;<br /> &lt;ref name='PMID:17329260'/&gt;<br /> &lt;ref name='PMID:17456599'/&gt;<br /> &lt;ref name='PMID:17726096'/&gt;<br /> &lt;ref name='PMID:16885328'/&gt;<br /> &lt;ref name='PMID:15170217'/&gt;<br /> &lt;ref name='PMID:15241471'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg <br /> 541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt <br /> 601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat <br /> 661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct <br /> 721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt <br /> 781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc <br /> 841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc <br /> 901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa <br /> 961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca <br /> 1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat <br /> 1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc <br /> 1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca <br /> 1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag <br /> 1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc <br /> 1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt <br /> 1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt <br /> 1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat <br /> 1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat <br /> 1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct <br /> 1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc <br /> 1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc <br /> 1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc <br /> 1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt <br /> 1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc <br /> 1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac <br /> 1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga <br /> 2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag <br /> 2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt <br /> 2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc <br /> 2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg <br /> 2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc <br /> 2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt <br /> 2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt <br /> 2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt <br /> 2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc <br /> 2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca <br /> 2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga <br /> 2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag <br /> 2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg <br /> 2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca <br /> 2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc <br /> 2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt <br /> 3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta <br /> 3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg <br /> 3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca <br /> 3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa <br /> 3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac <br /> 3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc <br /> 3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga <br /> 3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt <br /> 3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg <br /> 3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca <br /> 3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac <br /> 3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt <br /> 3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa <br /> 3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg <br /> 3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc <br /> 3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc <br /> 3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Chloramphenicol Resistant Marker]][[Category:T7 Promoter]][[Category:S Tag]][[Category:His Tag]][[Category:MCS]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pETDuet-1&diff=328541 pETDuet-1 2007-12-13T09:09:27Z <p>Amy: /* Notes */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PETDUET-1.jpg|thumb|250px|right|Image of pETDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> ColE1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> ampicillin&lt;br&gt;carbenicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71146-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;His•Tag coding sequence&lt;br&gt;Multiple cloning sites (Nco I–Afl II) &lt;br&gt;S•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla (Ap) coding sequence&lt;br&gt;f1 origin&lt;br&gt;<br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be used in combination with pACYCDuet-1 in an appropriate host strain for the coexpression of up to 4 target genes.The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. Use the Duet vectors together or with existing pET, pCDF, pRSF, and pETcoco™ recombinants with compatible replicons and antibiotic resistance to coexpress up to eight proteins in the same cell&lt;ref&gt;Novy, R., et all. 2002. ''inNovations '''''15''',2.&lt;/ref&gt;&lt;ref&gt;Held, D., et al. 2003. ''inNovations'' '''18''', 4.&lt;/ref&gt;.<br /> <br /> ==History ==<br /> pETDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:17269935'/&gt;<br /> &lt;ref name='PMID:17804794'/&gt;<br /> &lt;ref name='PMID:17726096'/&gt;<br /> &lt;ref name='PMID:16672227'/&gt;<br /> &lt;ref name='PMID:16339145'/&gt;<br /> &lt;ref name='PMID:17003130'/&gt;<br /> &lt;ref name='PMID:16369554'/&gt;<br /> &lt;ref name='PMID:17001002'/&gt;<br /> &lt;ref name='PMID:16263725'/&gt;<br /> &lt;ref name='PMID:16107877'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga <br /> 541 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt <br /> 601 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct <br /> 661 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg <br /> 721 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag <br /> 781 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa <br /> 841 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga <br /> 901 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa <br /> 961 atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg <br /> 1021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca <br /> 1081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca <br /> 1141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag <br /> 1201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac <br /> 1261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc <br /> 1321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct <br /> 1381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc <br /> 1441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg <br /> 1501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc <br /> 1561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat <br /> 1621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag <br /> 1681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat <br /> 1741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg <br /> 1801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca <br /> 1861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga <br /> 1921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc <br /> 1981 ttcctttttc aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat <br /> 2041 atttgaatgt atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt <br /> 2101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt <br /> 2161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt <br /> 2221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc <br /> 2281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg <br /> 2341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg <br /> 2401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt <br /> 2461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac <br /> 2521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg <br /> 2581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg <br /> 2641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat <br /> 2701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt <br /> 2761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg <br /> 2821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa <br /> 2881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc <br /> 2941 tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg <br /> 3001 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg <br /> 3061 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg <br /> 3121 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca <br /> 3181 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc <br /> 3241 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt <br /> 3301 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact <br /> 3361 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag <br /> 3421 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag <br /> 3481 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc <br /> 3541 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg <br /> 3601 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca <br /> 3661 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg <br /> 3721 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag <br /> 3781 cctagccggg tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg <br /> 3841 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga <br /> 3901 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg <br /> 3961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg <br /> 4021 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc <br /> 4081 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc <br /> 4141 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc <br /> 4201 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc <br /> 4261 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat <br /> 4321 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg <br /> 4381 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac <br /> 4441 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc <br /> 4501 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc <br /> 4561 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc <br /> 4621 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg <br /> 4681 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc <br /> 4741 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc <br /> 4801 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac <br /> 4861 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc <br /> 4921 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata <br /> 4981 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc <br /> 5041 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc <br /> 5101 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt <br /> 5161 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg <br /> 5221 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag <br /> 5281 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc <br /> 5341 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga <br /> 5401 aattaatacg actcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Novagen Plasmids]][[Category:Expression Plasmids]][[Category:Ampicillin Resistant Marker]][[Category:T7 Promoter]][[Category:S Tag]][[Category:His Tag]][[Category:MCS]][[Category:Carbenicillin]]<br /> <br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pACYCDuet-1&diff=328540 pACYCDuet-1 2007-12-13T09:06:13Z <p>Amy: /* At a Glance {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PACYCDUET-1.jpg|thumb|250px|right|Image of pACYCDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> P15A<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> 10-12<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> chloramphenicol<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71147-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;His•Tag coding sequence&lt;br&gt;Multiple cloning sites<br /> (Nco I–Afl II)&lt;br&gt;S•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;P15A origin&lt;br&gt;cat (CmR) coding sequence&lt;br&gt;lacI coding sequence <br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be transformed into the same cell with pETDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes.<br /> <br /> ==History ==<br /> pACYCDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:17269935'/&gt;<br /> &lt;ref name='PMID:17804794'/&gt;<br /> &lt;ref name='PMID:16369554'/&gt;<br /> &lt;ref name='PMID:17060320'/&gt;<br /> &lt;ref name='PMID:17329260'/&gt;<br /> &lt;ref name='PMID:17456599'/&gt;<br /> &lt;ref name='PMID:17726096'/&gt;<br /> &lt;ref name='PMID:16885328'/&gt;<br /> &lt;ref name='PMID:15170217'/&gt;<br /> &lt;ref name='PMID:15241471'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg <br /> 541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt <br /> 601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat <br /> 661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct <br /> 721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt <br /> 781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc <br /> 841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc <br /> 901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa <br /> 961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca <br /> 1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat <br /> 1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc <br /> 1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca <br /> 1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag <br /> 1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc <br /> 1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt <br /> 1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt <br /> 1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat <br /> 1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat <br /> 1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct <br /> 1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc <br /> 1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc <br /> 1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc <br /> 1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt <br /> 1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc <br /> 1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac <br /> 1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga <br /> 2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag <br /> 2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt <br /> 2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc <br /> 2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg <br /> 2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc <br /> 2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt <br /> 2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt <br /> 2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt <br /> 2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc <br /> 2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca <br /> 2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga <br /> 2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag <br /> 2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg <br /> 2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca <br /> 2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc <br /> 2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt <br /> 3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta <br /> 3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg <br /> 3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca <br /> 3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa <br /> 3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac <br /> 3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc <br /> 3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga <br /> 3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt <br /> 3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg <br /> 3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca <br /> 3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac <br /> 3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt <br /> 3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa <br /> 3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg <br /> 3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc <br /> 3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc <br /> 3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Chloramphenicol Resistant Marker]][[Category:T7 Promoter]][[Category:S Tag]][[Category:His Tag]][[Category:MCS]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pACYCDuet-1&diff=328539 pACYCDuet-1 2007-12-13T09:05:45Z <p>Amy: edited by Amy via TableEdit</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> [[Image:PACYCDUET-1.jpg|thumb|250px|right|Image of pACYCDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> P15A<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> 10-12<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> chloramphenicol<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71147-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;His•Tag coding sequence&lt;br&gt;Multiple cloning sites<br /> (Nco I–Afl II)&lt;br&gt;S•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;P15A origin&lt;br&gt;cat (CmR) coding sequence&lt;br&gt;lacI coding sequence <br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be transformed into the same cell with pETDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes.<br /> <br /> ==History ==<br /> pACYCDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:17269935'/&gt;<br /> &lt;ref name='PMID:17804794'/&gt;<br /> &lt;ref name='PMID:16369554'/&gt;<br /> &lt;ref name='PMID:17060320'/&gt;<br /> &lt;ref name='PMID:17329260'/&gt;<br /> &lt;ref name='PMID:17456599'/&gt;<br /> &lt;ref name='PMID:17726096'/&gt;<br /> &lt;ref name='PMID:16885328'/&gt;<br /> &lt;ref name='PMID:15170217'/&gt;<br /> &lt;ref name='PMID:15241471'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg <br /> 541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt <br /> 601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat <br /> 661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct <br /> 721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt <br /> 781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc <br /> 841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc <br /> 901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa <br /> 961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca <br /> 1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat <br /> 1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc <br /> 1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca <br /> 1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag <br /> 1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc <br /> 1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt <br /> 1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt <br /> 1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat <br /> 1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat <br /> 1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct <br /> 1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc <br /> 1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc <br /> 1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc <br /> 1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt <br /> 1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc <br /> 1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac <br /> 1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga <br /> 2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag <br /> 2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt <br /> 2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc <br /> 2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg <br /> 2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc <br /> 2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt <br /> 2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt <br /> 2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt <br /> 2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc <br /> 2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca <br /> 2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga <br /> 2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag <br /> 2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg <br /> 2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca <br /> 2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc <br /> 2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt <br /> 3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta <br /> 3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg <br /> 3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca <br /> 3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa <br /> 3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac <br /> 3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc <br /> 3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga <br /> 3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt <br /> 3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg <br /> 3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca <br /> 3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac <br /> 3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt <br /> 3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa <br /> 3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg <br /> 3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc <br /> 3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc <br /> 3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Chloramphenicol Resistant Marker]][[Category:T7 Promoter]][[Category:S Tag]][[Category:His Tag]][[Category:MCS]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pETDuet-1&diff=328538 pETDuet-1 2007-12-13T09:03:03Z <p>Amy: /* Sequence */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PETDUET-1.jpg|thumb|250px|right|Image of pETDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> ColE1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> ampicillin&lt;br&gt;carbenicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71146-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;His•Tag coding sequence&lt;br&gt;Multiple cloning sites (Nco I–Afl II) &lt;br&gt;S•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla (Ap) coding sequence&lt;br&gt;f1 origin&lt;br&gt;<br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be used in combination with pACYCDuet-1 in an appropriate host strain for the coexpression of up to 4 target genes.The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. Use the Duet vectors together or with existing pET, pCDF, pRSF, and pETcoco™ recombinants with compatible replicons and antibiotic resistance to coexpress up to eight proteins in the same cell<br /> <br /> ==History ==<br /> pETDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:17269935'/&gt;<br /> &lt;ref name='PMID:17804794'/&gt;<br /> &lt;ref name='PMID:17726096'/&gt;<br /> &lt;ref name='PMID:16672227'/&gt;<br /> &lt;ref name='PMID:16339145'/&gt;<br /> &lt;ref name='PMID:17003130'/&gt;<br /> &lt;ref name='PMID:16369554'/&gt;<br /> &lt;ref name='PMID:17001002'/&gt;<br /> &lt;ref name='PMID:16263725'/&gt;<br /> &lt;ref name='PMID:16107877'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga <br /> 541 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt <br /> 601 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct <br /> 661 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg <br /> 721 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag <br /> 781 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa <br /> 841 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga <br /> 901 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa <br /> 961 atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg <br /> 1021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca <br /> 1081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca <br /> 1141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag <br /> 1201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac <br /> 1261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc <br /> 1321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct <br /> 1381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc <br /> 1441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg <br /> 1501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc <br /> 1561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat <br /> 1621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag <br /> 1681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat <br /> 1741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg <br /> 1801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca <br /> 1861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga <br /> 1921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc <br /> 1981 ttcctttttc aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat <br /> 2041 atttgaatgt atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt <br /> 2101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt <br /> 2161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt <br /> 2221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc <br /> 2281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg <br /> 2341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg <br /> 2401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt <br /> 2461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac <br /> 2521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg <br /> 2581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg <br /> 2641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat <br /> 2701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt <br /> 2761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg <br /> 2821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa <br /> 2881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc <br /> 2941 tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg <br /> 3001 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg <br /> 3061 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg <br /> 3121 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca <br /> 3181 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc <br /> 3241 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt <br /> 3301 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact <br /> 3361 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag <br /> 3421 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag <br /> 3481 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc <br /> 3541 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg <br /> 3601 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca <br /> 3661 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg <br /> 3721 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag <br /> 3781 cctagccggg tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg <br /> 3841 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga <br /> 3901 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg <br /> 3961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg <br /> 4021 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc <br /> 4081 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc <br /> 4141 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc <br /> 4201 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc <br /> 4261 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat <br /> 4321 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg <br /> 4381 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac <br /> 4441 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc <br /> 4501 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc <br /> 4561 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc <br /> 4621 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg <br /> 4681 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc <br /> 4741 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc <br /> 4801 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac <br /> 4861 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc <br /> 4921 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata <br /> 4981 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc <br /> 5041 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc <br /> 5101 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt <br /> 5161 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg <br /> 5221 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag <br /> 5281 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc <br /> 5341 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga <br /> 5401 aattaatacg actcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Novagen Plasmids]][[Category:Expression Plasmids]][[Category:Ampicillin Resistant Marker]][[Category:T7 Promoter]][[Category:S Tag]][[Category:His Tag]][[Category:MCS]][[Category:Carbenicillin]]<br /> <br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pETDuet-1&diff=328537 pETDuet-1 2007-12-13T09:01:42Z <p>Amy: edited by Amy via TableEdit</p> <hr /> <div>==At a Glance ==<br /> [[Image:PETDUET-1.jpg|thumb|250px|right|Image of pETDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> ColE1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> ampicillin&lt;br&gt;carbenicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71146-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;His•Tag coding sequence&lt;br&gt;Multiple cloning sites (Nco I–Afl II) &lt;br&gt;S•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla (Ap) coding sequence&lt;br&gt;f1 origin&lt;br&gt;<br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be used in combination with pACYCDuet-1 in an appropriate host strain for the coexpression of up to 4 target genes.The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. Use the Duet vectors together or with existing pET, pCDF, pRSF, and pETcoco™ recombinants with compatible replicons and antibiotic resistance to coexpress up to eight proteins in the same cell<br /> <br /> ==History ==<br /> pETDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:17269935'/&gt;<br /> &lt;ref name='PMID:17804794'/&gt;<br /> &lt;ref name='PMID:17726096'/&gt;<br /> &lt;ref name='PMID:16672227'/&gt;<br /> &lt;ref name='PMID:16339145'/&gt;<br /> &lt;ref name='PMID:17003130'/&gt;<br /> &lt;ref name='PMID:16369554'/&gt;<br /> &lt;ref name='PMID:17001002'/&gt;<br /> &lt;ref name='PMID:16263725'/&gt;<br /> &lt;ref name='PMID:16107877'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga <br /> 541 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt <br /> 601 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct <br /> 661 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg <br /> 721 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag <br /> 781 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa <br /> 841 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga <br /> 901 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa <br /> 961 atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg <br /> 1021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca <br /> 1081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca <br /> 1141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag <br /> 1201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac <br /> 1261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc <br /> 1321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct <br /> 1381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc <br /> 1441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg <br /> 1501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc <br /> 1561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat <br /> 1621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag <br /> 1681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat <br /> 1741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg <br /> 1801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca <br /> 1861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga <br /> 1921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc <br /> 1981 ttcctttttc aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat <br /> 2041 atttgaatgt atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt <br /> 2101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt <br /> 2161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt <br /> 2221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc <br /> 2281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg <br /> 2341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg <br /> 2401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt <br /> 2461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac <br /> 2521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg <br /> 2581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg <br /> 2641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat <br /> 2701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt <br /> 2761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg <br /> 2821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa <br /> 2881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc <br /> 2941 tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg <br /> 3001 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg <br /> 3061 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg <br /> 3121 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca <br /> 3181 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc <br /> 3241 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt <br /> 3301 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact <br /> 3361 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag <br /> 3421 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag <br /> 3481 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc <br /> 3541 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg <br /> 3601 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca <br /> 3661 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg <br /> 3721 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag <br /> 3781 cctagccggg tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg <br /> 3841 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga <br /> 3901 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg <br /> 3961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg <br /> 4021 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc <br /> 4081 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc <br /> 4141 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc <br /> 4201 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc <br /> 4261 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat <br /> 4321 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg <br /> 4381 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac <br /> 4441 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc <br /> 4501 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc <br /> 4561 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc <br /> 4621 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg <br /> 4681 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc <br /> 4741 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc <br /> 4801 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac <br /> 4861 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc <br /> 4921 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata <br /> 4981 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc <br /> 5041 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc <br /> 5101 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt <br /> 5161 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg <br /> 5221 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag <br /> 5281 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc <br /> 5341 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga <br /> 5401 aattaatacg actcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Novagen Plasmids]][[Category:Expression Plasmids]][[Category:Ampicillin Resistant Marker]][[Category:T7 Promoter]][[Category:S Tag]][[Category:His Tag]][[Category:MCS]]<br /> <br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pACYCDuet-1&diff=328536 pACYCDuet-1 2007-12-13T08:57:23Z <p>Amy: /* Sequence */</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> [[Image:PACYCDUET-1.jpg|thumb|250px|right|Image of pACYCDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> P15A<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> 10-12<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> chloramphenicol<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71147-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter-1&lt;br&gt;T7 transcription start-1&lt;br&gt;His•Tag® coding sequence&lt;br&gt;Multiple cloning sites-1<br /> (Nco I–Afl II)&lt;br&gt;T7 promoter-2&lt;br&gt;T7 transcription start-2&lt;br&gt;Multiple cloning sites-2<br /> (Nde I–Avr II)&lt;br&gt;S•Tag™coding sequence&lt;br&gt;T7 terminator&lt;br&gt;P15A origin&lt;br&gt;cat (CmR) coding sequence&lt;br&gt;lacI coding sequence <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be transformed into the same cell with pETDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes.<br /> <br /> ==History ==<br /> pACYCDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:17269935'/&gt;<br /> &lt;ref name='PMID:17804794'/&gt;<br /> &lt;ref name='PMID:16369554'/&gt;<br /> &lt;ref name='PMID:17060320'/&gt;<br /> &lt;ref name='PMID:17329260'/&gt;<br /> &lt;ref name='PMID:17456599'/&gt;<br /> &lt;ref name='PMID:17726096'/&gt;<br /> &lt;ref name='PMID:16885328'/&gt;<br /> &lt;ref name='PMID:15170217'/&gt;<br /> &lt;ref name='PMID:15241471'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg <br /> 541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt <br /> 601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat <br /> 661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct <br /> 721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt <br /> 781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc <br /> 841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc <br /> 901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa <br /> 961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca <br /> 1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat <br /> 1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc <br /> 1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca <br /> 1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag <br /> 1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc <br /> 1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt <br /> 1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt <br /> 1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat <br /> 1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat <br /> 1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct <br /> 1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc <br /> 1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc <br /> 1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc <br /> 1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt <br /> 1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc <br /> 1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac <br /> 1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga <br /> 2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag <br /> 2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt <br /> 2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc <br /> 2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg <br /> 2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc <br /> 2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt <br /> 2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt <br /> 2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt <br /> 2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc <br /> 2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca <br /> 2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga <br /> 2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag <br /> 2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg <br /> 2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca <br /> 2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc <br /> 2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt <br /> 3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta <br /> 3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg <br /> 3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca <br /> 3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa <br /> 3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac <br /> 3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc <br /> 3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga <br /> 3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt <br /> 3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg <br /> 3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca <br /> 3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac <br /> 3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt <br /> 3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa <br /> 3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg <br /> 3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc <br /> 3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc <br /> 3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:Chloramphenicol Resistant Marker]][[Category:T7 Promoter]][[Category:S Tag]][[Category:His Tag]][[Category:MCS]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pACYCDuet-1&diff=328535 pACYCDuet-1 2007-12-13T08:53:44Z <p>Amy: /* References */</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> [[Image:PACYCDUET-1.jpg|thumb|250px|right|Image of pACYCDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> P15A<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> 10-12<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> chloramphenicol<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71147-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter-1&lt;br&gt;T7 transcription start-1&lt;br&gt;His•Tag® coding sequence&lt;br&gt;Multiple cloning sites-1<br /> (Nco I–Afl II)&lt;br&gt;T7 promoter-2&lt;br&gt;T7 transcription start-2&lt;br&gt;Multiple cloning sites-2<br /> (Nde I–Avr II)&lt;br&gt;S•Tag™coding sequence&lt;br&gt;T7 terminator&lt;br&gt;P15A origin&lt;br&gt;cat (CmR) coding sequence&lt;br&gt;lacI coding sequence <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be transformed into the same cell with pETDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes.<br /> <br /> ==History ==<br /> pACYCDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:17269935'/&gt;<br /> &lt;ref name='PMID:17804794'/&gt;<br /> &lt;ref name='PMID:16369554'/&gt;<br /> &lt;ref name='PMID:17060320'/&gt;<br /> &lt;ref name='PMID:17329260'/&gt;<br /> &lt;ref name='PMID:17456599'/&gt;<br /> &lt;ref name='PMID:17726096'/&gt;<br /> &lt;ref name='PMID:16885328'/&gt;<br /> &lt;ref name='PMID:15170217'/&gt;<br /> &lt;ref name='PMID:15241471'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg <br /> 541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt <br /> 601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat <br /> 661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct <br /> 721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt <br /> 781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc <br /> 841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc <br /> 901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa <br /> 961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca <br /> 1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat <br /> 1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc <br /> 1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca <br /> 1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag <br /> 1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc <br /> 1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt <br /> 1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt <br /> 1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat <br /> 1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat <br /> 1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct <br /> 1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc <br /> 1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc <br /> 1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc <br /> 1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt <br /> 1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc <br /> 1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac <br /> 1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga <br /> 2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag <br /> 2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt <br /> 2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc <br /> 2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg <br /> 2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc <br /> 2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt <br /> 2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt <br /> 2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt <br /> 2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc <br /> 2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca <br /> 2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga <br /> 2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag <br /> 2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg <br /> 2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca <br /> 2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc <br /> 2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt <br /> 3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta <br /> 3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg <br /> 3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca <br /> 3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa <br /> 3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac <br /> 3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc <br /> 3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga <br /> 3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt <br /> 3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg <br /> 3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca <br /> 3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac <br /> 3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt <br /> 3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa <br /> 3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg <br /> 3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc <br /> 3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc <br /> 3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pETDuet-1&diff=328534 pETDuet-1 2007-12-13T08:44:37Z <p>Amy: /* Sequence */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PETDUET-1.jpg|thumb|250px|right|Image of pETDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> ColE1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> ampicillin&lt;br&gt;carbenicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71146-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter-1&lt;br&gt;T7 transcription start-1&lt;br&gt;His•Tag® coding sequence&lt;br&gt;Multiple cloning sites-1 (Nco I–Afl II) &lt;br&gt;T7 promoter-2&lt;br&gt;T7 transcription start-2&lt;br&gt;Multiple cloning sites-2 (Nde I–Avr II) &lt;br&gt;S•Tag™coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla (Ap) coding sequence&lt;br&gt;f1 origin&lt;br&gt;<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be used in combination with pACYCDuet-1 in an appropriate host strain for the coexpression of up to 4 target genes.The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. Use the Duet vectors together or with existing pET, pCDF, pRSF, and pETcoco™ recombinants with compatible replicons and antibiotic resistance to coexpress up to eight proteins in the same cell<br /> <br /> ==History ==<br /> pETDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:17269935'/&gt;<br /> &lt;ref name='PMID:17804794'/&gt;<br /> &lt;ref name='PMID:17726096'/&gt;<br /> &lt;ref name='PMID:16672227'/&gt;<br /> &lt;ref name='PMID:16339145'/&gt;<br /> &lt;ref name='PMID:17003130'/&gt;<br /> &lt;ref name='PMID:16369554'/&gt;<br /> &lt;ref name='PMID:17001002'/&gt;<br /> &lt;ref name='PMID:16263725'/&gt;<br /> &lt;ref name='PMID:16107877'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga <br /> 541 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt <br /> 601 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct <br /> 661 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg <br /> 721 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag <br /> 781 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa <br /> 841 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga <br /> 901 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa <br /> 961 atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg <br /> 1021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca <br /> 1081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca <br /> 1141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag <br /> 1201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac <br /> 1261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc <br /> 1321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct <br /> 1381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc <br /> 1441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg <br /> 1501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc <br /> 1561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat <br /> 1621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag <br /> 1681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat <br /> 1741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg <br /> 1801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca <br /> 1861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga <br /> 1921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc <br /> 1981 ttcctttttc aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat <br /> 2041 atttgaatgt atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt <br /> 2101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt <br /> 2161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt <br /> 2221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc <br /> 2281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg <br /> 2341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg <br /> 2401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt <br /> 2461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac <br /> 2521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg <br /> 2581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg <br /> 2641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat <br /> 2701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt <br /> 2761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg <br /> 2821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa <br /> 2881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc <br /> 2941 tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg <br /> 3001 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg <br /> 3061 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg <br /> 3121 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca <br /> 3181 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc <br /> 3241 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt <br /> 3301 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact <br /> 3361 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag <br /> 3421 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag <br /> 3481 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc <br /> 3541 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg <br /> 3601 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca <br /> 3661 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg <br /> 3721 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag <br /> 3781 cctagccggg tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg <br /> 3841 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga <br /> 3901 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg <br /> 3961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg <br /> 4021 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc <br /> 4081 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc <br /> 4141 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc <br /> 4201 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc <br /> 4261 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat <br /> 4321 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg <br /> 4381 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac <br /> 4441 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc <br /> 4501 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc <br /> 4561 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc <br /> 4621 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg <br /> 4681 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc <br /> 4741 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc <br /> 4801 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac <br /> 4861 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc <br /> 4921 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata <br /> 4981 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc <br /> 5041 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc <br /> 5101 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt <br /> 5161 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg <br /> 5221 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag <br /> 5281 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc <br /> 5341 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga <br /> 5401 aattaatacg actcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Novagen Plasmids]][[Category:Expression Plasmids]][[Category:Ampicillin Resistant Marker]][[Category:T7 Promoter]][[Category:S Tag]][[Category:His Tag]][[Category:MCS]]<br /> <br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pETDuet-1&diff=328533 pETDuet-1 2007-12-13T08:43:27Z <p>Amy: /* Sequence */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PETDUET-1.jpg|thumb|250px|right|Image of pETDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> ColE1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> ampicillin&lt;br&gt;carbenicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71146-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter-1&lt;br&gt;T7 transcription start-1&lt;br&gt;His•Tag® coding sequence&lt;br&gt;Multiple cloning sites-1 (Nco I–Afl II) &lt;br&gt;T7 promoter-2&lt;br&gt;T7 transcription start-2&lt;br&gt;Multiple cloning sites-2 (Nde I–Avr II) &lt;br&gt;S•Tag™coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla (Ap) coding sequence&lt;br&gt;f1 origin&lt;br&gt;<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be used in combination with pACYCDuet-1 in an appropriate host strain for the coexpression of up to 4 target genes.The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. Use the Duet vectors together or with existing pET, pCDF, pRSF, and pETcoco™ recombinants with compatible replicons and antibiotic resistance to coexpress up to eight proteins in the same cell<br /> <br /> ==History ==<br /> pETDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:17269935'/&gt;<br /> &lt;ref name='PMID:17804794'/&gt;<br /> &lt;ref name='PMID:17726096'/&gt;<br /> &lt;ref name='PMID:16672227'/&gt;<br /> &lt;ref name='PMID:16339145'/&gt;<br /> &lt;ref name='PMID:17003130'/&gt;<br /> &lt;ref name='PMID:16369554'/&gt;<br /> &lt;ref name='PMID:17001002'/&gt;<br /> &lt;ref name='PMID:16263725'/&gt;<br /> &lt;ref name='PMID:16107877'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga <br /> 541 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt <br /> 601 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct <br /> 661 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg <br /> 721 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag <br /> 781 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa <br /> 841 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga <br /> 901 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa <br /> 961 atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg <br /> 1021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca <br /> 1081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca <br /> 1141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag <br /> 1201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac <br /> 1261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc <br /> 1321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct <br /> 1381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc <br /> 1441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg <br /> 1501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc <br /> 1561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat <br /> 1621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag <br /> 1681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat <br /> 1741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg <br /> 1801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca <br /> 1861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga <br /> 1921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc <br /> 1981 ttcctttttc aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat <br /> 2041 atttgaatgt atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt <br /> 2101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt <br /> 2161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt <br /> 2221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc <br /> 2281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg <br /> 2341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg <br /> 2401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt <br /> 2461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac <br /> 2521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg <br /> 2581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg <br /> 2641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat <br /> 2701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt <br /> 2761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg <br /> 2821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa <br /> 2881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc <br /> 2941 tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg <br /> 3001 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg <br /> 3061 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg <br /> 3121 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca <br /> 3181 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc <br /> 3241 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt <br /> 3301 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact <br /> 3361 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag <br /> 3421 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag <br /> 3481 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc <br /> 3541 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg <br /> 3601 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca <br /> 3661 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg <br /> 3721 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag <br /> 3781 cctagccggg tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg <br /> 3841 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga <br /> 3901 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg <br /> 3961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg <br /> 4021 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc <br /> 4081 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc <br /> 4141 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc <br /> 4201 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc <br /> 4261 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat <br /> 4321 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg <br /> 4381 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac <br /> 4441 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc <br /> 4501 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc <br /> 4561 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc <br /> 4621 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg <br /> 4681 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc <br /> 4741 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc <br /> 4801 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac <br /> 4861 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc <br /> 4921 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata <br /> 4981 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc <br /> 5041 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc <br /> 5101 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt <br /> 5161 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg <br /> 5221 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag <br /> 5281 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc <br /> 5341 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga <br /> 5401 aattaatacg actcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Ampicillin Resistant Marker]][[Category:T7 Promoter]][[Category:S Tag]][[Category:His Tag]][[Category:MCS]]<br /> <br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pETDuet-1&diff=328532 pETDuet-1 2007-12-13T08:40:50Z <p>Amy: /* References */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PETDUET-1.jpg|thumb|250px|right|Image of pETDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> ColE1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> ampicillin&lt;br&gt;carbenicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71146-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter-1&lt;br&gt;T7 transcription start-1&lt;br&gt;His•Tag® coding sequence&lt;br&gt;Multiple cloning sites-1 (Nco I–Afl II) &lt;br&gt;T7 promoter-2&lt;br&gt;T7 transcription start-2&lt;br&gt;Multiple cloning sites-2 (Nde I–Avr II) &lt;br&gt;S•Tag™coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla (Ap) coding sequence&lt;br&gt;f1 origin&lt;br&gt;<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be used in combination with pACYCDuet-1 in an appropriate host strain for the coexpression of up to 4 target genes.The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. Use the Duet vectors together or with existing pET, pCDF, pRSF, and pETcoco™ recombinants with compatible replicons and antibiotic resistance to coexpress up to eight proteins in the same cell<br /> <br /> ==History ==<br /> pETDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:17269935'/&gt;<br /> &lt;ref name='PMID:17804794'/&gt;<br /> &lt;ref name='PMID:17726096'/&gt;<br /> &lt;ref name='PMID:16672227'/&gt;<br /> &lt;ref name='PMID:16339145'/&gt;<br /> &lt;ref name='PMID:17003130'/&gt;<br /> &lt;ref name='PMID:16369554'/&gt;<br /> &lt;ref name='PMID:17001002'/&gt;<br /> &lt;ref name='PMID:16263725'/&gt;<br /> &lt;ref name='PMID:16107877'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga <br /> 541 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt <br /> 601 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct <br /> 661 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg <br /> 721 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag <br /> 781 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa <br /> 841 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga <br /> 901 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa <br /> 961 atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg <br /> 1021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca <br /> 1081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca <br /> 1141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag <br /> 1201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac <br /> 1261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc <br /> 1321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct <br /> 1381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc <br /> 1441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg <br /> 1501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc <br /> 1561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat <br /> 1621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag <br /> 1681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat <br /> 1741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg <br /> 1801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca <br /> 1861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga <br /> 1921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc <br /> 1981 ttcctttttc aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat <br /> 2041 atttgaatgt atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt <br /> 2101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt <br /> 2161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt <br /> 2221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc <br /> 2281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg <br /> 2341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg <br /> 2401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt <br /> 2461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac <br /> 2521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg <br /> 2581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg <br /> 2641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat <br /> 2701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt <br /> 2761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg <br /> 2821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa <br /> 2881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc <br /> 2941 tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg <br /> 3001 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg <br /> 3061 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg <br /> 3121 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca <br /> 3181 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc <br /> 3241 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt <br /> 3301 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact <br /> 3361 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag <br /> 3421 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag <br /> 3481 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc <br /> 3541 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg <br /> 3601 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca <br /> 3661 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg <br /> 3721 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag <br /> 3781 cctagccggg tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg <br /> 3841 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga <br /> 3901 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg <br /> 3961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg <br /> 4021 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc <br /> 4081 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc <br /> 4141 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc <br /> 4201 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc <br /> 4261 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat <br /> 4321 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg <br /> 4381 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac <br /> 4441 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc <br /> 4501 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc <br /> 4561 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc <br /> 4621 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg <br /> 4681 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc <br /> 4741 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc <br /> 4801 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac <br /> 4861 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc <br /> 4921 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata <br /> 4981 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc <br /> 5041 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc <br /> 5101 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt <br /> 5161 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg <br /> 5221 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag <br /> 5281 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc <br /> 5341 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga <br /> 5401 aattaatacg actcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pETDuet-1&diff=328531 pETDuet-1 2007-12-13T08:12:45Z <p>Amy: /* Notes */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PETDUET-1.jpg|thumb|250px|right|Image of pETDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> ColE1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> ampicillin&lt;br&gt;carbenicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71146-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter-1&lt;br&gt;T7 transcription start-1&lt;br&gt;His•Tag® coding sequence&lt;br&gt;Multiple cloning sites-1 (Nco I–Afl II) &lt;br&gt;T7 promoter-2&lt;br&gt;T7 transcription start-2&lt;br&gt;Multiple cloning sites-2 (Nde I–Avr II) &lt;br&gt;S•Tag™coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla (Ap) coding sequence&lt;br&gt;f1 origin&lt;br&gt;<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be used in combination with pACYCDuet-1 in an appropriate host strain for the coexpression of up to 4 target genes.The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. Use the Duet vectors together or with existing pET, pCDF, pRSF, and pETcoco™ recombinants with compatible replicons and antibiotic resistance to coexpress up to eight proteins in the same cell<br /> <br /> ==History ==<br /> pETDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga <br /> 541 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt <br /> 601 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct <br /> 661 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg <br /> 721 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag <br /> 781 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa <br /> 841 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga <br /> 901 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa <br /> 961 atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg <br /> 1021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca <br /> 1081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca <br /> 1141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag <br /> 1201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac <br /> 1261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc <br /> 1321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct <br /> 1381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc <br /> 1441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg <br /> 1501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc <br /> 1561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat <br /> 1621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag <br /> 1681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat <br /> 1741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg <br /> 1801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca <br /> 1861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga <br /> 1921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc <br /> 1981 ttcctttttc aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat <br /> 2041 atttgaatgt atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt <br /> 2101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt <br /> 2161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt <br /> 2221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc <br /> 2281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg <br /> 2341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg <br /> 2401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt <br /> 2461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac <br /> 2521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg <br /> 2581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg <br /> 2641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat <br /> 2701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt <br /> 2761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg <br /> 2821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa <br /> 2881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc <br /> 2941 tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg <br /> 3001 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg <br /> 3061 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg <br /> 3121 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca <br /> 3181 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc <br /> 3241 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt <br /> 3301 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact <br /> 3361 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag <br /> 3421 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag <br /> 3481 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc <br /> 3541 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg <br /> 3601 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca <br /> 3661 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg <br /> 3721 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag <br /> 3781 cctagccggg tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg <br /> 3841 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga <br /> 3901 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg <br /> 3961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg <br /> 4021 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc <br /> 4081 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc <br /> 4141 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc <br /> 4201 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc <br /> 4261 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat <br /> 4321 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg <br /> 4381 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac <br /> 4441 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc <br /> 4501 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc <br /> 4561 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc <br /> 4621 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg <br /> 4681 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc <br /> 4741 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc <br /> 4801 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac <br /> 4861 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc <br /> 4921 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata <br /> 4981 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc <br /> 5041 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc <br /> 5101 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt <br /> 5161 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg <br /> 5221 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag <br /> 5281 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc <br /> 5341 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga <br /> 5401 aattaatacg actcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pACYCDuet-1&diff=328530 pACYCDuet-1 2007-12-13T08:11:16Z <p>Amy: /* History {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> [[Image:PACYCDUET-1.jpg|thumb|250px|right|Image of pACYCDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> P15A<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> 10-12<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> chloramphenicol<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71147-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter-1&lt;br&gt;T7 transcription start-1&lt;br&gt;His•Tag® coding sequence&lt;br&gt;Multiple cloning sites-1<br /> (Nco I–Afl II)&lt;br&gt;T7 promoter-2&lt;br&gt;T7 transcription start-2&lt;br&gt;Multiple cloning sites-2<br /> (Nde I–Avr II)&lt;br&gt;S•Tag™coding sequence&lt;br&gt;T7 terminator&lt;br&gt;P15A origin&lt;br&gt;cat (CmR) coding sequence&lt;br&gt;lacI coding sequence <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be transformed into the same cell with pETDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes.<br /> <br /> ==History ==<br /> pACYCDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg <br /> 541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt <br /> 601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat <br /> 661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct <br /> 721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt <br /> 781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc <br /> 841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc <br /> 901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa <br /> 961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca <br /> 1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat <br /> 1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc <br /> 1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca <br /> 1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag <br /> 1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc <br /> 1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt <br /> 1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt <br /> 1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat <br /> 1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat <br /> 1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct <br /> 1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc <br /> 1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc <br /> 1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc <br /> 1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt <br /> 1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc <br /> 1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac <br /> 1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga <br /> 2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag <br /> 2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt <br /> 2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc <br /> 2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg <br /> 2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc <br /> 2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt <br /> 2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt <br /> 2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt <br /> 2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc <br /> 2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca <br /> 2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga <br /> 2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag <br /> 2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg <br /> 2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca <br /> 2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc <br /> 2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt <br /> 3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta <br /> 3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg <br /> 3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca <br /> 3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa <br /> 3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac <br /> 3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc <br /> 3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga <br /> 3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt <br /> 3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg <br /> 3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca <br /> 3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac <br /> 3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt <br /> 3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa <br /> 3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg <br /> 3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc <br /> 3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc <br /> 3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pACYCDuet-1&diff=328529 pACYCDuet-1 2007-12-13T08:10:15Z <p>Amy: /* Notes */</p> <hr /> <div>==At a Glance {{HelpIcon|Help:Plasmid}}==<br /> [[Image:PACYCDUET-1.jpg|thumb|250px|right|Image of pACYCDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> P15A<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> 10-12<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> chloramphenicol<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71147-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter-1&lt;br&gt;T7 transcription start-1&lt;br&gt;His•Tag® coding sequence&lt;br&gt;Multiple cloning sites-1<br /> (Nco I–Afl II)&lt;br&gt;T7 promoter-2&lt;br&gt;T7 transcription start-2&lt;br&gt;Multiple cloning sites-2<br /> (Nde I–Avr II)&lt;br&gt;S•Tag™coding sequence&lt;br&gt;T7 terminator&lt;br&gt;P15A origin&lt;br&gt;cat (CmR) coding sequence&lt;br&gt;lacI coding sequence <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.W474a644916d5e--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be transformed into the same cell with pETDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes.<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB336.pdf Novagen]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg <br /> 541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt <br /> 601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat <br /> 661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct <br /> 721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt <br /> 781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc <br /> 841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc <br /> 901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa <br /> 961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca <br /> 1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat <br /> 1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc <br /> 1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca <br /> 1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag <br /> 1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc <br /> 1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt <br /> 1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt <br /> 1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat <br /> 1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat <br /> 1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct <br /> 1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc <br /> 1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc <br /> 1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc <br /> 1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt <br /> 1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc <br /> 1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac <br /> 1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga <br /> 2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag <br /> 2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt <br /> 2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc <br /> 2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg <br /> 2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc <br /> 2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt <br /> 2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt <br /> 2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt <br /> 2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc <br /> 2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca <br /> 2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga <br /> 2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag <br /> 2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg <br /> 2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca <br /> 2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc <br /> 2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt <br /> 3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta <br /> 3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg <br /> 3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca <br /> 3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa <br /> 3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac <br /> 3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc <br /> 3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga <br /> 3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt <br /> 3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg <br /> 3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca <br /> 3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac <br /> 3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt <br /> 3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa <br /> 3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg <br /> 3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc <br /> 3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc <br /> 3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pBiEx-1&diff=328528 pBiEx-1 2007-12-13T08:03:58Z <p>Amy: /* Notes */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PBIEX-1.jpg|thumb|250px|right|Image of pBiEx-1. [http://www.merckbiosciences.com/docs/docs/PROT/TB354-000.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.R475ff9fe776f6--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pUC<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> High<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.merckbiosciences.com/docs/NDIS/71234-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> hr5 enhancer &lt;br&gt;IE1 promoter &lt;br&gt;IE1 transcription start &lt;br&gt;T7 promoter &lt;br&gt;T7 transcription start &lt;br&gt;His•Tag® coding sequence &lt;br&gt;S•Tag™coding sequence &lt;br&gt;Multiple cloning sites (Bgl II–Dra III) &lt;br&gt;HSV•Tag® coding sequence &lt;br&gt;T7 terminator &lt;br&gt;IE1 terminator &lt;br&gt;lacI coding sequence &lt;br&gt;bla (Ap) coding sequence &lt;br&gt;pUC origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.R475ff9fe776f6&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.R475ff9fe776f6--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> The vectors feature the same hr5 enhancer and the ie1 (immediate early) promoter to direct expression in insect cells as the pIEx™ Vectors, and also allow for expression in E. coli by the tightly controlled T7lac promoter.<br /> <br /> ==History ==<br /> The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/product/71234 Novagen]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat <br /> 61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca <br /> 121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg <br /> 181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata <br /> 241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac <br /> 301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg <br /> 361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa <br /> 421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac <br /> 481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg <br /> 541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg <br /> 601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag <br /> 661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg <br /> 721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga <br /> 781 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta <br /> 841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg <br /> 901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca <br /> 961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt <br /> 1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg <br /> 1081 accgcggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct <br /> 1141 agaaataatt ttgtttaact ttaagaagga gatataccat ggcacaccat caccaccatc <br /> 1201 actcttctgg taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgccac <br /> 1261 cgccttctgg tctggtcccc cggggcagcg caggttctgg tacgattgat gacgacgaca <br /> 1321 agatctctgg cccgggctcg gatcccaatt gggagctcgt gtacacggcg cgcctgcagg <br /> 1381 tcgacggtac cggttcgaag cttgcggccg cacagctgta tacacgtgca agccagccag <br /> 1441 aactcgcccc ggaagacccc gaggatctcg agcactaagt gattaacctc agccgaaagg <br /> 1501 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta <br /> 1561 aacgggtctt gaggggtttt ttgctgaaag gaggaactat cctcaggtta tacatatatt <br /> 1621 ttgaatttaa ttaattatac atatatttta tattattttt gtcttttatt atcgaggggc <br /> 1681 cgttgttggt gtggggtttt gcatagaaat aacaatggga gttggcgacg ttgctgcgcc <br /> 1741 aacaccacct cccttccctc ctttcatcat gtatctgtag ataaaataaa atattaaacc <br /> 1801 taaaaacaag accgcgccta tcaacaaaat gataggcatt aacttgccgc tgacgctgtc <br /> 1861 actaacgttg gacgatttgc cgactaaacc ttcatcgccc agtaaccaat ctagacgtca <br /> 1921 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca <br /> 1981 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac <br /> 2041 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg <br /> 2101 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg <br /> 2161 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg <br /> 2221 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat <br /> 2281 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg <br /> 2341 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca <br /> 2401 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc <br /> 2461 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag <br /> 2521 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc <br /> 2581 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa <br /> 2641 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg <br /> 2701 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca <br /> 2761 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt <br /> 2821 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg <br /> 2881 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac <br /> 2941 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc <br /> 3001 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa <br /> 3061 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca <br /> 3121 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg <br /> 3181 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa <br /> 3241 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatggac <br /> 3301 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat <br /> 3361 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg <br /> 3421 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc <br /> 3481 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga <br /> 3541 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga <br /> 3601 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg <br /> 3661 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc <br /> 3721 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac <br /> 3781 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact <br /> 3841 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca <br /> 3901 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg <br /> 3961 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact <br /> 4021 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg <br /> 4081 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg <br /> 4141 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat <br /> 4201 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc <br /> 4261 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat <br /> 4321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt <br /> 4381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc <br /> 4441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt <br /> 4501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac <br /> 4561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt <br /> 4621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct <br /> 4681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga <br /> 4741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac <br /> 4801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg <br /> 4861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt <br /> 4921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc <br /> 4981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg <br /> 5041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc <br /> 5101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc <br /> 5161 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag <br /> 5221 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca <br /> 5281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat <br /> 5341 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg <br /> 5401 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga <br /> 5461 ttacgaattc ccggg <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:T7 Promoter]][[Category:IE1 Promoter]][[Category:HSV Tag]][[Category:His Tag]][[Category:S Tag]][[Category:Ampicillin Marker Plasmids]][[Category:hr5 Enhancer]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pBiEx-2&diff=328527 pBiEx-2 2007-12-13T08:02:46Z <p>Amy: /* Notes */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PBIEX-2.jpg|thumb|250px|right|Image of pBiEx-2. [http://www.merckbiosciences.com/docs/docs/PROT/TB353.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.R475ffa1b02581--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pUC<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> High<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.merckbiosciences.com/docs/NDIS/71233-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> hr5 enhancer &lt;br&gt;IE1 promoter &lt;br&gt;IE1 transcription start &lt;br&gt;T7 promoter &lt;br&gt;T7 transcription start &lt;br&gt;GST•Tag™ coding sequence &lt;br&gt;His•Tag® coding sequence &lt;br&gt;S•Tag™coding sequence &lt;br&gt;Multiple cloning sites (Bgl II–Dra III) &lt;br&gt;HSV•Tag® coding sequence &lt;br&gt;T7 terminator &lt;br&gt;IE1 terminator &lt;br&gt;lacI &lt;br&gt;bla (Ap) coding sequence&lt;br&gt;pUC origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.R475ffa1b02581&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.R475ffa1b02581--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> The vectors feature the same hr5 enhancer and the ie1 (immediate early) promoter to direct expression in insect cells as the pIEx™ Vectors, and also allow for expression in E. coli by the tightly controlled T7lac promoter.<br /> <br /> ==History ==<br /> The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/product/71233 Novagen]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat <br /> 61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca <br /> 121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg <br /> 181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata <br /> 241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac <br /> 301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg <br /> 361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa <br /> 421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac <br /> 481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg <br /> 541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg <br /> 601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag <br /> 661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg <br /> 721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga <br /> 781 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta <br /> 841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg <br /> 901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca <br /> 961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt <br /> 1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg <br /> 1081 accgcggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct <br /> 1141 agaaataatt ttgtttaact ttaagaagga gatataccat ggcatcccct atactaggtt <br /> 1201 attggaaaat taagggcctt gtgcaaccca ctcgacttct tttggaatat cttgaagaaa <br /> 1261 aatatgaaga gcatttgtat gagcgcgatg aaggtgataa atggcgaaac aaaaagtttg <br /> 1321 aattgggttt ggagtttccc aatcttcctt attatattga tggtgatgtt aaattaacac <br /> 1381 agtctatggc catcatacgt tatatagctg acaagcacaa catgttgggt ggttgtccaa <br /> 1441 aagagcgtgc agagatttca atgcttgaag gagcggtttt ggatattaga tacggtgttt <br /> 1501 cgagaattgc atatagtaaa gactttgaaa ctctcaaagt tgattttctt agcaagctac <br /> 1561 ctgaaatgct gaaaatgttc gaagatcgtt tatgtcataa aacatattta aatggtgatc <br /> 1621 atgtaaccca tcctgacttc atgttgtatg acgctcttga tgttgtttta tacatggacc <br /> 1681 caatgtgcct ggatgcgttc ccaaaattag tttgttttaa aaaacgtatt gaagctatcc <br /> 1741 cacaaattga taagtacttg aaatccagca agtatatagc atggcctttg cagggctggc <br /> 1801 aagccacgtt tggtggtggc gaccatcctc caaaatcgga tggttcaact agtggttctg <br /> 1861 gttccatggc acaccatcac caccatcact cttctggtaa agaaaccgct gctgcgaaat <br /> 1921 ttgaacgcca gcacatggac tcgccaccgc cttctggtct ggtcccccgg ggcagcgcag <br /> 1981 gttctggtac gattgatgac gacgacaaga tctctggccc gggctcggat cccaattggg <br /> 2041 agctcgtgta cacggcgcgc ctgcaggtcg acggtaccgg ttcgaagctt gcggccgcac <br /> 2101 agctgtatac acgtgcaagc cagccagaac tcgccccgga agaccccgag gatctcgagc <br /> 2161 actaagtgat taacctcagc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat <br /> 2221 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag <br /> 2281 gaactatcct caggttatac atatattttg aatttaatta attatacata tattttatat <br /> 2341 tatttttgtc ttttattatc gaggggccgt tgttggtgtg gggttttgca tagaaataac <br /> 2401 aatgggagtt ggcgacgttg ctgcgccaac accacctccc ttccctcctt tcatcatgta <br /> 2461 tctgtagata aaataaaata ttaaacctaa aaacaagacc gcgcctatca acaaaatgat <br /> 2521 aggcattaac ttgccgctga cgctgtcact aacgttggac gatttgccga ctaaaccttc <br /> 2581 atcgcccagt aaccaatcta gacgtcacga tagtcatgcc ccgcgcccac cggaaggagc <br /> 2641 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct <br /> 2701 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc <br /> 2761 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg <br /> 2821 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc <br /> 2881 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg <br /> 2941 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc <br /> 3001 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc <br /> 3061 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg <br /> 3121 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt <br /> 3181 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt <br /> 3241 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc <br /> 3301 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca <br /> 3361 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca <br /> 3421 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc <br /> 3481 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt <br /> 3541 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg <br /> 3601 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg <br /> 3661 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg <br /> 3721 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg <br /> 3781 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc <br /> 3841 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc <br /> 3901 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac <br /> 3961 cgccgccgca aggaatggtg catggacgtc aggtggcact tttcggggaa atgtgcgcgg <br /> 4021 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata <br /> 4081 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg <br /> 4141 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac <br /> 4201 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact <br /> 4261 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat <br /> 4321 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga <br /> 4381 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac <br /> 4441 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat <br /> 4501 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac <br /> 4561 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct <br /> 4621 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac <br /> 4681 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga <br /> 4741 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg <br /> 4801 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact <br /> 4861 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac <br /> 4921 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta <br /> 4981 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt <br /> 5041 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga <br /> 5101 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc <br /> 5161 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt <br /> 5221 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc <br /> 5281 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc <br /> 5341 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg <br /> 5401 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg <br /> 5461 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga <br /> 5521 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc <br /> 5581 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg <br /> 5641 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg <br /> 5701 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt <br /> 5761 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc <br /> 5821 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg <br /> 5881 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc <br /> 5941 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg <br /> 6001 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca <br /> 6061 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt <br /> 6121 tcacacagga aacagctatg accatgatta cgaattcccg gg <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:T7 Promoter]][[Category:IE1 Promoter]][[Category:HSV Tag]][[Category:His Tag]][[Category:S Tag]][[Category:Ampicillin Marker Plasmids]][[Category:hr5 Enhancer]][[Category:GST Tag]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pBiEx-3&diff=328526 pBiEx-3 2007-12-13T07:59:49Z <p>Amy: /* Notes */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PBIEX-3.jpg|thumb|250px|right|Image of pBiEx-3. [http://www.merckbiosciences.com/docs/docs/PROT/TB355.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.I475ffa3050321--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pUC<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> High<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.merckbiosciences.com/docs/NDIS/71232-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> hr5 enhancer &lt;br&gt;IE1 promoter &lt;br&gt;IE1 transcription start &lt;br&gt;T7 promoter &lt;br&gt;T7 transcription start &lt;br&gt;Multiple cloning sites (Nco I–Dra III) &lt;br&gt;S•Tag™coding sequence &lt;br&gt;His•Tag® coding sequence &lt;br&gt;T7 terminator &lt;br&gt;IE1 terminator &lt;br&gt;lacI coding sequence &lt;br&gt;bla (Ap) coding sequence &lt;br&gt;pUC origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.I475ffa3050321&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.I475ffa3050321--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> In insect cells, transcription is driven by the AcNPV-derived hr5<br /> enhancer and IE1 immediate early promoter.<br /> <br /> ==History ==<br /> The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/product/71232 Novagen]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat <br /> 61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca <br /> 121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg <br /> 181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata <br /> 241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac <br /> 301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg <br /> 361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa <br /> 421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac <br /> 481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg <br /> 541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg <br /> 601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag <br /> 661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg <br /> 721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga <br /> 781 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta <br /> 841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg <br /> 901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca <br /> 961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt <br /> 1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg <br /> 1081 accgcggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct <br /> 1141 agaaataatt ttgtttaact ttaagaagga gatataccat ggcatcgtta acacgtcaag <br /> 1201 agctcgcgga tcccaattgg cagatctcgg cgcgcctgca ggtcgacggt accggttcga <br /> 1261 agcttgcggc cgcatcttct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca <br /> 1321 tggactcgcc accgccttct ggcctcgagc accaccatca ccatcaccat cactaagtga <br /> 1381 ttaacctcag ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat <br /> 1441 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatcc <br /> 1501 tcaggttata catatatttt gaatttaatt aattatacat atattttata ttatttttgt <br /> 1561 cttttattat cgaggggccg ttgttggtgt ggggttttgc atagaaataa caatgggagt <br /> 1621 tggcgacgtt gctgcgccaa caccacctcc cttccctcct ttcatcatgt atctgtagat <br /> 1681 aaaataaaat attaaaccta aaaacaagac cgcgcctatc aacaaaatga taggcattaa <br /> 1741 cttgccgctg acgctgtcac taacgttgga cgatttgccg actaaacctt catcgcccag <br /> 1801 taaccaatct agacgtcacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt <br /> 1861 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat <br /> 1921 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt <br /> 1981 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt <br /> 2041 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt <br /> 2101 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt <br /> 2161 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc <br /> 2221 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg <br /> 2281 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga <br /> 2341 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga <br /> 2401 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc <br /> 2461 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta <br /> 2521 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat <br /> 2581 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga <br /> 2641 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag <br /> 2701 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg <br /> 2761 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca <br /> 2821 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa <br /> 2881 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc <br /> 2941 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat <br /> 3001 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc <br /> 3061 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg <br /> 3121 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc <br /> 3181 aaggaatggt gcatggacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat <br /> 3241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata <br /> 3301 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct <br /> 3361 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa <br /> 3421 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa <br /> 3481 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt <br /> 3541 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg <br /> 3601 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca <br /> 3661 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa <br /> 3721 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt <br /> 3781 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc <br /> 3841 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa <br /> 3901 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga <br /> 3961 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc <br /> 4021 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga <br /> 4081 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga <br /> 4141 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga <br /> 4201 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat <br /> 4261 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt <br /> 4321 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct <br /> 4381 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc <br /> 4441 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc <br /> 4501 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc <br /> 4561 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc <br /> 4621 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg <br /> 4681 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata <br /> 4741 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta <br /> 4801 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc <br /> 4861 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg <br /> 4921 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt <br /> 4981 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt <br /> 5041 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga <br /> 5101 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc <br /> 5161 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg <br /> 5221 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca <br /> 5281 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg <br /> 5341 aaacagctat gaccatgatt acgaattccc ggg<br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:T7 Promoter]][[Category:IE1 Promoter]][[Category:His Tag]][[Category:S Tag]][[Category:Ampicillin Marker Plasmids]][[Category:hr5 Enhancer]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pETBlue-2&diff=328525 pETBlue-2 2007-12-13T07:54:30Z <p>Amy: /* History */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PETBLUE-2.jpg|thumb|250px|right|Image of pETBlue-2. [http://www.merckbiosciences.com/docs/docs/PROT/TB259.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M475ff9fa4e4eb--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pUC, f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> high<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.merckbiosciences.com/docs/NDIS/70609-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> lac operator &lt;br&gt;T7 promoter &lt;br&gt;lac operator &lt;br&gt;T7 transcription start&lt;br&gt;multiple cloning region (Nco I–Pac I) &lt;br&gt; His•Tag® coding sequence &lt;br&gt;HSV•Tag® coding sequence &lt;br&gt;lacZ start codon &lt;br&gt;lacZ -peptide ORF &lt;br&gt;E. coli promoter &lt;br&gt;f1 origin &lt;br&gt;bla coding sequence &lt;br&gt;pUC origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.M475ff9fa4e4eb&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.M475ff9fa4e4eb--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> pETBlue-2 provides a vector-encoded ATG start codon and a variety of downstream cloning sites.<br /> <br /> ==History ==<br /> The pETBlue™ System combines the visual identification of recombinants and high plasmid copy number, found in popular blue/white screening vectors, with the tightly controlled high yield protein expression obtained with pET vectors.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/product/70673 Novagen]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gacttacaat <br /> 61 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtacg ggcctcttcg <br /> 121 ctattacgcc agcttgcgaa cggtgggtgc gctgcaaggc gattaagttg ggtaacgcca <br /> 181 ggattctccc agtcacgacg ttgtaaaacg acggccagcg agagatcttg attggctagc <br /> 241 agaataattt tgtttaactt taagaaggag atataccatg gcgatatccc gggagctcgt <br /> 301 ggatccgaat tctgtacagg cgcgcctgca ggacgtcgac ggtaccatcg atacgcgttc <br /> 361 gaagcttgcg gccgcacagc tgtatacacg tgcaagccag ccagaactcg ctcctgaaga <br /> 421 cccagaggat ctcgagcacc accaccacca ccactaatgt taattaagtt gggcgttgta <br /> 481 atcatagtca taatcaatac tcctgactgc gttagcaatt taactgtgat aaactaccgc <br /> 541 attaaagcta ttcgatgata agctgtcaaa catgataatt cttgaagacg aaagggccta <br /> 601 ggctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc <br /> 661 cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag <br /> 721 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt <br /> 781 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat <br /> 841 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc <br /> 901 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact <br /> 961 cttttgttta tttttctaaa tacattcaaa tatgtatccg ctgagcaata actagcataa <br /> 1021 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc <br /> 1081 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta <br /> 1141 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc <br /> 1201 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt <br /> 1261 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg <br /> 1321 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca <br /> 1381 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct <br /> 1441 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga <br /> 1501 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca <br /> 1561 cgatggcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag <br /> 1621 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat <br /> 1681 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc <br /> 1741 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat <br /> 1801 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag <br /> 1861 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg <br /> 1921 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc <br /> 1981 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca <br /> 2041 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg <br /> 2101 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc <br /> 2161 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta <br /> 2221 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc <br /> 2281 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg <br /> 2341 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc <br /> 2401 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc <br /> 2461 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat <br /> 2521 actcatactc ttcctttttc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac <br /> 2581 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc <br /> 2641 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat <br /> 2701 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat <br /> 2761 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct <br /> 2821 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt <br /> 2881 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg <br /> 2941 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta <br /> 3001 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg <br /> 3061 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg <br /> 3121 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc <br /> 3181 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg <br /> 3241 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat <br /> 3301 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc <br /> 3361 agcgagtcag tgagcgagga agccggcgat aatggcctgc ttctcgccga aacgtttggt <br /> 3421 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga <br /> 3481 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc <br /> 3541 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgac <br /> 3601 cggtgaattg tgagcgctca caattctcgt gacatcataa cgtcccgcga aat<br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:T7 Promoter]][[Category:Lac Operator]][[Category:HSV Tag]][[Category:His Tag]][[Category:E.coli Promoter]][[Category:Ampicillin Marker Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pETBlue-1&diff=328524 pETBlue-1 2007-12-13T07:52:07Z <p>Amy: /* History */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PETBLUE-1.jpg|thumb|250px|right|Image of pETBlue-1. [http://www.merckbiosciences.com/docs/docs/PROT/TB258.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U475ff9bc89d11--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> pUC, f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> high<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.merckbiosciences.com/docs/NDIS/70608-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> lac operator &lt;br&gt;T7 promoter &lt;br&gt;lac operator &lt;br&gt;T7 transcription start &lt;br&gt;multiple cloning region &lt;br&gt;(EcoR V–Srf I) lacZ start codon &lt;br&gt;lacZ -peptide &lt;br&gt;E. coli promoter &lt;br&gt;f1 origin &lt;br&gt;bla coding sequence &lt;br&gt;pUC origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.U475ff9bc89d11&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U475ff9bc89d11--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> pETBlue-1 facilitates the expression of unfused, native protein from inserts that encode an open reading frame at the 5′-end.<br /> <br /> ==History ==<br /> The pETBlue™ System combines the visual identification of recombinants and high plasmid copy number, found in popular blue/white screening vectors, with the tightly controlled high yield protein expression obtained with pET vectors.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/product/70673 Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:17652308'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gacttacaat <br /> 61 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtacg ggcctcttcg <br /> 121 ctattacgcc agcttgcgaa cggtgggtgc gctgcaaggc gattaagttg ggtaacgcca <br /> 181 ggattctccc agtcacgacg ttgtaaaacg acggccagcg agagatcttg attggctagc <br /> 241 agaataattt tgtttaactt taagaaggag atatagatat cgaattcctg cccgggcgtt <br /> 301 gtaatcatag tcataatcaa tactcctgac tgcgttagca atttaactgt gataaactac <br /> 361 cgcattaaag ctattcgatg ataagctgtc aaacatgata attcttgaag acgaaagggc <br /> 421 ctaggctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca <br /> 481 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga <br /> 541 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt <br /> 601 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg <br /> 661 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact <br /> 721 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa <br /> 781 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctgagca ataactagca <br /> 841 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata <br /> 901 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg <br /> 961 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct <br /> 1021 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc <br /> 1081 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg <br /> 1141 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt <br /> 1201 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg <br /> 1261 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc <br /> 1321 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttctggcg <br /> 1381 gcacgatggc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg <br /> 1441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt <br /> 1501 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact <br /> 1561 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat <br /> 1621 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg <br /> 1681 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg <br /> 1741 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat <br /> 1801 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc <br /> 1861 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt <br /> 1921 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc <br /> 1981 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga <br /> 2041 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc <br /> 2101 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa <br /> 2161 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta <br /> 2221 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg <br /> 2281 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg <br /> 2341 aatactcata ctcttccttt ttcaatcatg accaaaatcc cttaacgtga gttttcgttc <br /> 2401 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg <br /> 2461 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg <br /> 2521 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca <br /> 2581 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg <br /> 2641 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg <br /> 2701 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga <br /> 2761 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac <br /> 2821 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat <br /> 2881 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc <br /> 2941 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga <br /> 3001 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc <br /> 3061 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg <br /> 3121 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag <br /> 3181 cgcagcgagt cagtgagcga ggaagccggc gataatggcc tgcttctcgc cgaaacgttt <br /> 3241 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag <br /> 3301 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag <br /> 3361 cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac <br /> 3421 gaccggtgaa ttgtgagcgc tcacaattct cgtgacatca taacgtcccg cgaaat <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Expression Plasmids]][[Category:Novagen Plasmids]][[Category:T7 Promoter]][[Category:Lac Operator]][[Category:E.coli Promoter]][[Category:Ampicillin Marker Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pETDuet-1&diff=328523 pETDuet-1 2007-12-13T07:18:24Z <p>Amy: /* History {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PETDUET-1.jpg|thumb|250px|right|Image of pETDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> ColE1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> ampicillin&lt;br&gt;carbenicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71146-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter-1&lt;br&gt;T7 transcription start-1&lt;br&gt;His•Tag® coding sequence&lt;br&gt;Multiple cloning sites-1 (Nco I–Afl II) &lt;br&gt;T7 promoter-2&lt;br&gt;T7 transcription start-2&lt;br&gt;Multiple cloning sites-2 (Nde I–Avr II) &lt;br&gt;S•Tag™coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla (Ap) coding sequence&lt;br&gt;f1 origin&lt;br&gt;<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be used in<br /> combination with pACYCDuet-1 in an appropriate host strain for the coexpression of up to 4 target genes.<br /> <br /> ==History ==<br /> pETDuet-1 is designed for the coexpression of two target genes.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga <br /> 541 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt <br /> 601 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct <br /> 661 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg <br /> 721 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag <br /> 781 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa <br /> 841 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga <br /> 901 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa <br /> 961 atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg <br /> 1021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca <br /> 1081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca <br /> 1141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag <br /> 1201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac <br /> 1261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc <br /> 1321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct <br /> 1381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc <br /> 1441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg <br /> 1501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc <br /> 1561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat <br /> 1621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag <br /> 1681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat <br /> 1741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg <br /> 1801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca <br /> 1861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga <br /> 1921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc <br /> 1981 ttcctttttc aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat <br /> 2041 atttgaatgt atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt <br /> 2101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt <br /> 2161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt <br /> 2221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc <br /> 2281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg <br /> 2341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg <br /> 2401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt <br /> 2461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac <br /> 2521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg <br /> 2581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg <br /> 2641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat <br /> 2701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt <br /> 2761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg <br /> 2821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa <br /> 2881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc <br /> 2941 tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg <br /> 3001 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg <br /> 3061 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg <br /> 3121 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca <br /> 3181 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc <br /> 3241 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt <br /> 3301 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact <br /> 3361 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag <br /> 3421 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag <br /> 3481 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc <br /> 3541 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg <br /> 3601 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca <br /> 3661 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg <br /> 3721 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag <br /> 3781 cctagccggg tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg <br /> 3841 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga <br /> 3901 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg <br /> 3961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg <br /> 4021 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc <br /> 4081 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc <br /> 4141 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc <br /> 4201 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc <br /> 4261 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat <br /> 4321 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg <br /> 4381 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac <br /> 4441 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc <br /> 4501 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc <br /> 4561 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc <br /> 4621 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg <br /> 4681 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc <br /> 4741 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc <br /> 4801 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac <br /> 4861 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc <br /> 4921 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata <br /> 4981 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc <br /> 5041 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc <br /> 5101 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt <br /> 5161 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg <br /> 5221 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag <br /> 5281 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc <br /> 5341 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga <br /> 5401 aattaatacg actcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pETDuet-1&diff=328522 pETDuet-1 2007-12-13T07:13:11Z <p>Amy: /* At a Glance {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PETDUET-1.jpg|thumb|250px|right|Image of pETDuet-1. [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Expression<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> ColE1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> ampicillin&lt;br&gt;carbenicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/71146-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter-1&lt;br&gt;T7 transcription start-1&lt;br&gt;His•Tag® coding sequence&lt;br&gt;Multiple cloning sites-1 (Nco I–Afl II) &lt;br&gt;T7 promoter-2&lt;br&gt;T7 transcription start-2&lt;br&gt;Multiple cloning sites-2 (Nde I–Avr II) &lt;br&gt;S•Tag™coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla (Ap) coding sequence&lt;br&gt;f1 origin&lt;br&gt;<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.D474a63d380f7d--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> This vector can be used in<br /> combination with pACYCDuet-1 in an appropriate host strain for the coexpression of up to 4 target genes.<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB337.pdf Novagen]<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag <br /> 61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag <br /> 121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag <br /> 181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt <br /> 241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata <br /> 301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt <br /> 361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg <br /> 421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg <br /> 481 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga <br /> 541 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt <br /> 601 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct <br /> 661 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg <br /> 721 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag <br /> 781 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa <br /> 841 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga <br /> 901 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa <br /> 961 atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg <br /> 1021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca <br /> 1081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca <br /> 1141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag <br /> 1201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac <br /> 1261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc <br /> 1321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct <br /> 1381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc <br /> 1441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg <br /> 1501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc <br /> 1561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat <br /> 1621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag <br /> 1681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat <br /> 1741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg <br /> 1801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca <br /> 1861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga <br /> 1921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc <br /> 1981 ttcctttttc aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat <br /> 2041 atttgaatgt atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt <br /> 2101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt <br /> 2161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt <br /> 2221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc <br /> 2281 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg <br /> 2341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg <br /> 2401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt <br /> 2461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac <br /> 2521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg <br /> 2581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg <br /> 2641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat <br /> 2701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt <br /> 2761 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg <br /> 2821 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa <br /> 2881 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc <br /> 2941 tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg <br /> 3001 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg <br /> 3061 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg <br /> 3121 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca <br /> 3181 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc <br /> 3241 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt <br /> 3301 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact <br /> 3361 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag <br /> 3421 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag <br /> 3481 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc <br /> 3541 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg <br /> 3601 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca <br /> 3661 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg <br /> 3721 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag <br /> 3781 cctagccggg tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg <br /> 3841 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga <br /> 3901 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg <br /> 3961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg <br /> 4021 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc <br /> 4081 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc <br /> 4141 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc <br /> 4201 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc <br /> 4261 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat <br /> 4321 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg <br /> 4381 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac <br /> 4441 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc <br /> 4501 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc <br /> 4561 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc <br /> 4621 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg <br /> 4681 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc <br /> 4741 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc <br /> 4801 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac <br /> 4861 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc <br /> 4921 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata <br /> 4981 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc <br /> 5041 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc <br /> 5101 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt <br /> 5161 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg <br /> 5221 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag <br /> 5281 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc <br /> 5341 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga <br /> 5401 aattaatacg actcactata <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-52_3c/LIC&diff=328521 pET-52 3c/LIC 2007-12-13T07:10:10Z <p>Amy: /* Sequence */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET523CLIC.jpg|thumb|250px|right|Image of pET-52 3c/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB438.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-52b-3CLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Strep•Tag II coding sequence&lt;br&gt;His•Tag coding sequence&lt;br&gt;<br /> T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 ori&lt;br&gt;bla (AmpR)&lt;br&gt;f1 origin<br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert.The<br /> pET-52b(+) 3C/LIC vector carries an N-terminal Strep•Tag II coding sequence &lt;ref name='PMID:9344409'/&gt; followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specific for cleavage of the sequence LEVLFQ↓GP &lt;ref name='PMID:2555540'/&gt;, and is active at low temperatures &lt;ref name='PMID:9344409'/&gt;. The 3C/LIC cloning site is followed by an optional thrombin recognition site and a C-terminal His•Tag coding sequence.<br /> <br /> ==History ==<br /> The pET-52 3C/LIC vector is a 3C/LIC version of pET-52b(+).<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/product/71571 Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:9344409'/&gt;<br /> &lt;ref name='PMID:2555540'/&gt;<br /> &lt;ref name='PMID:11036648'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg <br /> 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc <br /> 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg <br /> 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc <br /> 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt <br /> 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc <br /> 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta <br /> 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat <br /> 481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt <br /> 541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt <br /> 601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc <br /> 661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg <br /> 721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc <br /> 781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg <br /> 841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca <br /> 901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga <br /> 961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct <br /> 1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg <br /> 1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca <br /> 1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata <br /> 1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct <br /> 1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact <br /> 1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa <br /> 1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc <br /> 1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg <br /> 1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac <br /> 1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag <br /> 1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg <br /> 1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca <br /> 1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga <br /> 1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca <br /> 1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc <br /> 1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca <br /> 1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa <br /> 2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc <br /> 2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc <br /> 2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg <br /> 2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat <br /> 2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca <br /> 2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt <br /> 2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac <br /> 2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg <br /> 2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg <br /> 2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg <br /> 2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg <br /> 2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga <br /> 2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg <br /> 2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa <br /> 2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt <br /> 2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt <br /> 3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct <br /> 3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac <br /> 3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag <br /> 3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc <br /> 3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc <br /> 3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc <br /> 3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga <br /> 3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt <br /> 3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt <br /> 3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg <br /> 3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc <br /> 3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat <br /> 3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt <br /> 3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc <br /> 3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc <br /> 3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag <br /> 3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt <br /> 4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat <br /> 4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag <br /> 4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac <br /> 4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg <br /> 4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg <br /> 4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt <br /> 4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc <br /> 4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact <br /> 4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg <br /> 4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga <br /> 4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc <br /> 4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt <br /> 4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg <br /> 4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc <br /> 4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag <br /> 4921 aaataatttt gtttaacttt aagaaggaga tataccatgg caagctggag ccacccgcag <br /> 4981 ttcgaaaagg gtgcacttga agtcctcttt cagggacccg ggtaccagga tcctgtacaa <br /> 5041 gtcgacgcgg ccgcagagct cgctctggtg ccacgcggta gttccgctca tcaccaccat <br /> 5101 catcaccatc accaccacta attaacctag gctgctgcca ccgctgagca ataactagca <br /> 5161 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata <br /> 5221 tccggat <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]][[Category:Ampicillin Resistant Marker]][[Category:T7 Promoter]][[Category:Strep Tag]][[Category:His Tag]]<br /> <br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-52_3c/LIC&diff=328520 pET-52 3c/LIC 2007-12-13T07:06:31Z <p>Amy: /* Sources {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET523CLIC.jpg|thumb|250px|right|Image of pET-52 3c/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB438.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-52b-3CLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Strep•Tag II coding sequence&lt;br&gt;His•Tag coding sequence&lt;br&gt;<br /> T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 ori&lt;br&gt;bla (AmpR)&lt;br&gt;f1 origin<br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert.The<br /> pET-52b(+) 3C/LIC vector carries an N-terminal Strep•Tag II coding sequence &lt;ref name='PMID:9344409'/&gt; followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specific for cleavage of the sequence LEVLFQ↓GP &lt;ref name='PMID:2555540'/&gt;, and is active at low temperatures &lt;ref name='PMID:9344409'/&gt;. The 3C/LIC cloning site is followed by an optional thrombin recognition site and a C-terminal His•Tag coding sequence.<br /> <br /> ==History ==<br /> The pET-52 3C/LIC vector is a 3C/LIC version of pET-52b(+).<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/product/71571 Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:9344409'/&gt;<br /> &lt;ref name='PMID:2555540'/&gt;<br /> &lt;ref name='PMID:11036648'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg <br /> 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc <br /> 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg <br /> 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc <br /> 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt <br /> 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc <br /> 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta <br /> 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat <br /> 481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt <br /> 541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt <br /> 601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc <br /> 661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg <br /> 721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc <br /> 781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg <br /> 841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca <br /> 901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga <br /> 961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct <br /> 1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg <br /> 1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca <br /> 1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata <br /> 1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct <br /> 1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact <br /> 1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa <br /> 1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc <br /> 1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg <br /> 1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac <br /> 1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag <br /> 1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg <br /> 1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca <br /> 1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga <br /> 1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca <br /> 1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc <br /> 1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca <br /> 1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa <br /> 2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc <br /> 2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc <br /> 2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg <br /> 2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat <br /> 2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca <br /> 2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt <br /> 2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac <br /> 2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg <br /> 2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg <br /> 2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg <br /> 2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg <br /> 2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga <br /> 2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg <br /> 2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa <br /> 2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt <br /> 2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt <br /> 3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct <br /> 3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac <br /> 3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag <br /> 3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc <br /> 3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc <br /> 3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc <br /> 3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga <br /> 3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt <br /> 3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt <br /> 3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg <br /> 3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc <br /> 3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat <br /> 3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt <br /> 3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc <br /> 3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc <br /> 3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag <br /> 3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt <br /> 4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat <br /> 4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag <br /> 4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac <br /> 4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg <br /> 4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg <br /> 4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt <br /> 4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc <br /> 4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact <br /> 4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg <br /> 4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga <br /> 4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc <br /> 4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt <br /> 4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg <br /> 4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc <br /> 4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag <br /> 4921 aaataatttt gtttaacttt aagaaggaga tataccatgg caagctggag ccacccgcag <br /> 4981 ttcgaaaagg gtgcacttga agtcctcttt cagggacccg ggtaccagga tcctgtacaa <br /> 5041 gtcgacgcgg ccgcagagct cgctctggtg ccacgcggta gttccgctca tcaccaccat <br /> 5101 catcaccatc accaccacta attaacctag gctgctgcca ccgctgagca ataactagca <br /> 5161 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata <br /> 5221 tccggat <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-52_3c/LIC&diff=328519 pET-52 3c/LIC 2007-12-13T07:05:26Z <p>Amy: /* History {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET523CLIC.jpg|thumb|250px|right|Image of pET-52 3c/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB438.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-52b-3CLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Strep•Tag II coding sequence&lt;br&gt;His•Tag coding sequence&lt;br&gt;<br /> T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 ori&lt;br&gt;bla (AmpR)&lt;br&gt;f1 origin<br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert.The<br /> pET-52b(+) 3C/LIC vector carries an N-terminal Strep•Tag II coding sequence &lt;ref name='PMID:9344409'/&gt; followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specific for cleavage of the sequence LEVLFQ↓GP &lt;ref name='PMID:2555540'/&gt;, and is active at low temperatures &lt;ref name='PMID:9344409'/&gt;. The 3C/LIC cloning site is followed by an optional thrombin recognition site and a C-terminal His•Tag coding sequence.<br /> <br /> ==History ==<br /> The pET-52 3C/LIC vector is a 3C/LIC version of pET-52b(+).<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> &lt;ref name='PMID:9344409'/&gt;<br /> &lt;ref name='PMID:2555540'/&gt;<br /> &lt;ref name='PMID:11036648'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg <br /> 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc <br /> 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg <br /> 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc <br /> 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt <br /> 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc <br /> 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta <br /> 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat <br /> 481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt <br /> 541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt <br /> 601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc <br /> 661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg <br /> 721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc <br /> 781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg <br /> 841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca <br /> 901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga <br /> 961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct <br /> 1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg <br /> 1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca <br /> 1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata <br /> 1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct <br /> 1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact <br /> 1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa <br /> 1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc <br /> 1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg <br /> 1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac <br /> 1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag <br /> 1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg <br /> 1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca <br /> 1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga <br /> 1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca <br /> 1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc <br /> 1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca <br /> 1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa <br /> 2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc <br /> 2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc <br /> 2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg <br /> 2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat <br /> 2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca <br /> 2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt <br /> 2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac <br /> 2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg <br /> 2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg <br /> 2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg <br /> 2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg <br /> 2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga <br /> 2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg <br /> 2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa <br /> 2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt <br /> 2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt <br /> 3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct <br /> 3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac <br /> 3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag <br /> 3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc <br /> 3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc <br /> 3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc <br /> 3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga <br /> 3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt <br /> 3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt <br /> 3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg <br /> 3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc <br /> 3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat <br /> 3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt <br /> 3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc <br /> 3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc <br /> 3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag <br /> 3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt <br /> 4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat <br /> 4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag <br /> 4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac <br /> 4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg <br /> 4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg <br /> 4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt <br /> 4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc <br /> 4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact <br /> 4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg <br /> 4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga <br /> 4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc <br /> 4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt <br /> 4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg <br /> 4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc <br /> 4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag <br /> 4921 aaataatttt gtttaacttt aagaaggaga tataccatgg caagctggag ccacccgcag <br /> 4981 ttcgaaaagg gtgcacttga agtcctcttt cagggacccg ggtaccagga tcctgtacaa <br /> 5041 gtcgacgcgg ccgcagagct cgctctggtg ccacgcggta gttccgctca tcaccaccat <br /> 5101 catcaccatc accaccacta attaacctag gctgctgcca ccgctgagca ataactagca <br /> 5161 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata <br /> 5221 tccggat <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-52_3c/LIC&diff=328518 pET-52 3c/LIC 2007-12-13T07:04:15Z <p>Amy: /* Notes */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET523CLIC.jpg|thumb|250px|right|Image of pET-52 3c/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB438.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-52b-3CLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Strep•Tag II coding sequence&lt;br&gt;His•Tag coding sequence&lt;br&gt;<br /> T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 ori&lt;br&gt;bla (AmpR)&lt;br&gt;f1 origin<br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert.The<br /> pET-52b(+) 3C/LIC vector carries an N-terminal Strep•Tag II coding sequence &lt;ref name='PMID:9344409'/&gt; followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specific for cleavage of the sequence LEVLFQ↓GP &lt;ref name='PMID:2555540'/&gt;, and is active at low temperatures &lt;ref name='PMID:9344409'/&gt;. The 3C/LIC cloning site is followed by an optional thrombin recognition site and a C-terminal His•Tag coding sequence.<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> &lt;ref name='PMID:9344409'/&gt;<br /> &lt;ref name='PMID:2555540'/&gt;<br /> &lt;ref name='PMID:11036648'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg <br /> 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc <br /> 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg <br /> 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc <br /> 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt <br /> 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc <br /> 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta <br /> 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat <br /> 481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt <br /> 541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt <br /> 601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc <br /> 661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg <br /> 721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc <br /> 781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg <br /> 841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca <br /> 901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga <br /> 961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct <br /> 1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg <br /> 1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca <br /> 1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata <br /> 1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct <br /> 1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact <br /> 1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa <br /> 1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc <br /> 1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg <br /> 1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac <br /> 1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag <br /> 1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg <br /> 1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca <br /> 1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga <br /> 1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca <br /> 1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc <br /> 1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca <br /> 1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa <br /> 2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc <br /> 2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc <br /> 2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg <br /> 2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat <br /> 2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca <br /> 2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt <br /> 2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac <br /> 2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg <br /> 2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg <br /> 2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg <br /> 2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg <br /> 2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga <br /> 2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg <br /> 2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa <br /> 2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt <br /> 2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt <br /> 3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct <br /> 3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac <br /> 3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag <br /> 3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc <br /> 3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc <br /> 3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc <br /> 3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga <br /> 3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt <br /> 3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt <br /> 3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg <br /> 3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc <br /> 3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat <br /> 3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt <br /> 3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc <br /> 3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc <br /> 3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag <br /> 3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt <br /> 4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat <br /> 4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag <br /> 4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac <br /> 4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg <br /> 4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg <br /> 4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt <br /> 4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc <br /> 4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact <br /> 4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg <br /> 4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga <br /> 4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc <br /> 4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt <br /> 4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg <br /> 4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc <br /> 4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag <br /> 4921 aaataatttt gtttaacttt aagaaggaga tataccatgg caagctggag ccacccgcag <br /> 4981 ttcgaaaagg gtgcacttga agtcctcttt cagggacccg ggtaccagga tcctgtacaa <br /> 5041 gtcgacgcgg ccgcagagct cgctctggtg ccacgcggta gttccgctca tcaccaccat <br /> 5101 catcaccatc accaccacta attaacctag gctgctgcca ccgctgagca ataactagca <br /> 5161 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata <br /> 5221 tccggat <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-52_3c/LIC&diff=328517 pET-52 3c/LIC 2007-12-13T07:02:43Z <p>Amy: /* References */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET523CLIC.jpg|thumb|250px|right|Image of pET-52 3c/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB438.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-52b-3CLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Strep•Tag II coding sequence&lt;br&gt;His•Tag coding sequence&lt;br&gt;<br /> T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 ori&lt;br&gt;bla (AmpR)&lt;br&gt;f1 origin<br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert.The<br /> pET-52b(+) 3C/LIC vector carries an N-terminal Strep•Tag II coding sequence (1) followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specific for cleavage of the sequence LEVLFQ↓GP (2), and is active at low temperatures (3). The 3C/LIC cloning site is followed by an optional thrombin recognition site and a C-terminal His•Tag coding sequence.<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> &lt;ref name='PMID:9344409'/&gt;<br /> &lt;ref name='PMID:2555540'/&gt;<br /> &lt;ref name='PMID:11036648'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg <br /> 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc <br /> 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg <br /> 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc <br /> 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt <br /> 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc <br /> 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta <br /> 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat <br /> 481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt <br /> 541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt <br /> 601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc <br /> 661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg <br /> 721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc <br /> 781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg <br /> 841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca <br /> 901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga <br /> 961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct <br /> 1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg <br /> 1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca <br /> 1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata <br /> 1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct <br /> 1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact <br /> 1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa <br /> 1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc <br /> 1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg <br /> 1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac <br /> 1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag <br /> 1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg <br /> 1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca <br /> 1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga <br /> 1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca <br /> 1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc <br /> 1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca <br /> 1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa <br /> 2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc <br /> 2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc <br /> 2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg <br /> 2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat <br /> 2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca <br /> 2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt <br /> 2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac <br /> 2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg <br /> 2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg <br /> 2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg <br /> 2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg <br /> 2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga <br /> 2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg <br /> 2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa <br /> 2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt <br /> 2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt <br /> 3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct <br /> 3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac <br /> 3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag <br /> 3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc <br /> 3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc <br /> 3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc <br /> 3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga <br /> 3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt <br /> 3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt <br /> 3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg <br /> 3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc <br /> 3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat <br /> 3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt <br /> 3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc <br /> 3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc <br /> 3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag <br /> 3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt <br /> 4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat <br /> 4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag <br /> 4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac <br /> 4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg <br /> 4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg <br /> 4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt <br /> 4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc <br /> 4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact <br /> 4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg <br /> 4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga <br /> 4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc <br /> 4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt <br /> 4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg <br /> 4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc <br /> 4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag <br /> 4921 aaataatttt gtttaacttt aagaaggaga tataccatgg caagctggag ccacccgcag <br /> 4981 ttcgaaaagg gtgcacttga agtcctcttt cagggacccg ggtaccagga tcctgtacaa <br /> 5041 gtcgacgcgg ccgcagagct cgctctggtg ccacgcggta gttccgctca tcaccaccat <br /> 5101 catcaccatc accaccacta attaacctag gctgctgcca ccgctgagca ataactagca <br /> 5161 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata <br /> 5221 tccggat <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-52_3c/LIC&diff=328516 pET-52 3c/LIC 2007-12-13T07:02:07Z <p>Amy: /* Notes */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET523CLIC.jpg|thumb|250px|right|Image of pET-52 3c/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB438.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-52b-3CLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Strep•Tag II coding sequence&lt;br&gt;His•Tag coding sequence&lt;br&gt;<br /> T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 ori&lt;br&gt;bla (AmpR)&lt;br&gt;f1 origin<br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert.The<br /> pET-52b(+) 3C/LIC vector carries an N-terminal Strep•Tag II coding sequence (1) followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specific for cleavage of the sequence LEVLFQ↓GP (2), and is active at low temperatures (3). The 3C/LIC cloning site is followed by an optional thrombin recognition site and a C-terminal His•Tag coding sequence.<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> &lt;ref name='PMID:12538913'/&gt;<br /> &lt;ref name='PMID:9344409'/&gt;<br /> &lt;ref name='PMID:2555540'/&gt;<br /> &lt;ref name='PMID:11036648'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg <br /> 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc <br /> 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg <br /> 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc <br /> 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt <br /> 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc <br /> 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta <br /> 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat <br /> 481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt <br /> 541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt <br /> 601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc <br /> 661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg <br /> 721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc <br /> 781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg <br /> 841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca <br /> 901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga <br /> 961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct <br /> 1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg <br /> 1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca <br /> 1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata <br /> 1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct <br /> 1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact <br /> 1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa <br /> 1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc <br /> 1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg <br /> 1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac <br /> 1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag <br /> 1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg <br /> 1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca <br /> 1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga <br /> 1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca <br /> 1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc <br /> 1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca <br /> 1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa <br /> 2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc <br /> 2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc <br /> 2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg <br /> 2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat <br /> 2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca <br /> 2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt <br /> 2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac <br /> 2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg <br /> 2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg <br /> 2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg <br /> 2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg <br /> 2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga <br /> 2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg <br /> 2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa <br /> 2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt <br /> 2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt <br /> 3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct <br /> 3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac <br /> 3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag <br /> 3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc <br /> 3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc <br /> 3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc <br /> 3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga <br /> 3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt <br /> 3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt <br /> 3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg <br /> 3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc <br /> 3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat <br /> 3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt <br /> 3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc <br /> 3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc <br /> 3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag <br /> 3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt <br /> 4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat <br /> 4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag <br /> 4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac <br /> 4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg <br /> 4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg <br /> 4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt <br /> 4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc <br /> 4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact <br /> 4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg <br /> 4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga <br /> 4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc <br /> 4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt <br /> 4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg <br /> 4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc <br /> 4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag <br /> 4921 aaataatttt gtttaacttt aagaaggaga tataccatgg caagctggag ccacccgcag <br /> 4981 ttcgaaaagg gtgcacttga agtcctcttt cagggacccg ggtaccagga tcctgtacaa <br /> 5041 gtcgacgcgg ccgcagagct cgctctggtg ccacgcggta gttccgctca tcaccaccat <br /> 5101 catcaccatc accaccacta attaacctag gctgctgcca ccgctgagca ataactagca <br /> 5161 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata <br /> 5221 tccggat <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-52_3c/LIC&diff=328515 pET-52 3c/LIC 2007-12-13T07:00:01Z <p>Amy: /* References */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET523CLIC.jpg|thumb|250px|right|Image of pET-52 3c/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB438.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-52b-3CLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Strep•Tag II coding sequence&lt;br&gt;His•Tag coding sequence&lt;br&gt;<br /> T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 ori&lt;br&gt;bla (AmpR)&lt;br&gt;f1 origin<br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert.<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> &lt;ref name='PMID:12538913'/&gt;<br /> &lt;ref name='PMID:9344409'/&gt;<br /> &lt;ref name='PMID:2555540'/&gt;<br /> &lt;ref name='PMID:11036648'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg <br /> 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc <br /> 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg <br /> 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc <br /> 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt <br /> 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc <br /> 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta <br /> 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat <br /> 481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt <br /> 541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt <br /> 601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc <br /> 661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg <br /> 721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc <br /> 781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg <br /> 841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca <br /> 901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga <br /> 961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct <br /> 1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg <br /> 1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca <br /> 1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata <br /> 1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct <br /> 1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact <br /> 1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa <br /> 1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc <br /> 1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg <br /> 1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac <br /> 1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag <br /> 1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg <br /> 1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca <br /> 1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga <br /> 1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca <br /> 1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc <br /> 1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca <br /> 1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa <br /> 2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc <br /> 2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc <br /> 2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg <br /> 2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat <br /> 2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca <br /> 2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt <br /> 2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac <br /> 2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg <br /> 2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg <br /> 2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg <br /> 2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg <br /> 2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga <br /> 2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg <br /> 2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa <br /> 2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt <br /> 2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt <br /> 3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct <br /> 3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac <br /> 3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag <br /> 3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc <br /> 3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc <br /> 3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc <br /> 3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga <br /> 3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt <br /> 3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt <br /> 3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg <br /> 3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc <br /> 3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat <br /> 3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt <br /> 3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc <br /> 3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc <br /> 3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag <br /> 3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt <br /> 4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat <br /> 4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag <br /> 4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac <br /> 4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg <br /> 4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg <br /> 4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt <br /> 4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc <br /> 4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact <br /> 4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg <br /> 4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga <br /> 4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc <br /> 4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt <br /> 4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg <br /> 4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc <br /> 4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag <br /> 4921 aaataatttt gtttaacttt aagaaggaga tataccatgg caagctggag ccacccgcag <br /> 4981 ttcgaaaagg gtgcacttga agtcctcttt cagggacccg ggtaccagga tcctgtacaa <br /> 5041 gtcgacgcgg ccgcagagct cgctctggtg ccacgcggta gttccgctca tcaccaccat <br /> 5101 catcaccatc accaccacta attaacctag gctgctgcca ccgctgagca ataactagca <br /> 5161 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata <br /> 5221 tccggat <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-52_3c/LIC&diff=328514 pET-52 3c/LIC 2007-12-13T06:46:52Z <p>Amy: /* Notes */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET523CLIC.jpg|thumb|250px|right|Image of pET-52 3c/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB438.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-52b-3CLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Strep•Tag II coding sequence&lt;br&gt;His•Tag coding sequence&lt;br&gt;<br /> T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 ori&lt;br&gt;bla (AmpR)&lt;br&gt;f1 origin<br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert.<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg <br /> 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc <br /> 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg <br /> 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc <br /> 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt <br /> 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc <br /> 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta <br /> 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat <br /> 481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt <br /> 541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt <br /> 601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc <br /> 661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg <br /> 721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc <br /> 781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg <br /> 841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca <br /> 901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga <br /> 961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct <br /> 1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg <br /> 1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca <br /> 1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata <br /> 1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct <br /> 1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact <br /> 1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa <br /> 1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc <br /> 1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg <br /> 1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac <br /> 1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag <br /> 1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg <br /> 1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca <br /> 1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga <br /> 1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca <br /> 1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc <br /> 1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca <br /> 1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa <br /> 2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc <br /> 2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc <br /> 2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg <br /> 2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat <br /> 2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca <br /> 2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt <br /> 2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac <br /> 2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg <br /> 2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg <br /> 2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg <br /> 2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg <br /> 2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga <br /> 2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg <br /> 2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa <br /> 2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt <br /> 2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt <br /> 3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct <br /> 3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac <br /> 3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag <br /> 3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc <br /> 3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc <br /> 3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc <br /> 3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga <br /> 3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt <br /> 3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt <br /> 3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg <br /> 3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc <br /> 3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat <br /> 3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt <br /> 3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc <br /> 3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc <br /> 3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag <br /> 3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt <br /> 4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat <br /> 4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag <br /> 4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac <br /> 4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg <br /> 4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg <br /> 4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt <br /> 4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc <br /> 4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact <br /> 4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg <br /> 4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga <br /> 4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc <br /> 4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt <br /> 4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg <br /> 4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc <br /> 4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag <br /> 4921 aaataatttt gtttaacttt aagaaggaga tataccatgg caagctggag ccacccgcag <br /> 4981 ttcgaaaagg gtgcacttga agtcctcttt cagggacccg ggtaccagga tcctgtacaa <br /> 5041 gtcgacgcgg ccgcagagct cgctctggtg ccacgcggta gttccgctca tcaccaccat <br /> 5101 catcaccatc accaccacta attaacctag gctgctgcca ccgctgagca ataactagca <br /> 5161 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata <br /> 5221 tccggat <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-52_3c/LIC&diff=328513 pET-52 3c/LIC 2007-12-13T06:11:52Z <p>Amy: /* At a Glance {{HelpIcon|Help:Plasmid}} */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET523CLIC.jpg|thumb|250px|right|Image of pET-52 3c/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB438.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/docs/LIT/pET-52b-3CLIC-gb.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Strep•Tag II coding sequence&lt;br&gt;His•Tag coding sequence&lt;br&gt;<br /> T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 ori&lt;br&gt;bla (AmpR)&lt;br&gt;f1 origin<br /> <br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.H474a63dd53324--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ===Notes===<br /> <br /> ==History {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidHistory}}<br /> <br /> ==Sources {{HelpIcon|Help:Plasmid}}==<br /> {{PlasmidSources}}<br /> <br /> ==References==<br /> {{RefHelp}}<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg <br /> 61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc <br /> 121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg <br /> 181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc <br /> 241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt <br /> 301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc <br /> 361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta <br /> 421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat <br /> 481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt <br /> 541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt <br /> 601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc <br /> 661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg <br /> 721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc <br /> 781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg <br /> 841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca <br /> 901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga <br /> 961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct <br /> 1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg <br /> 1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca <br /> 1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata <br /> 1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct <br /> 1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact <br /> 1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa <br /> 1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc <br /> 1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg <br /> 1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac <br /> 1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag <br /> 1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg <br /> 1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca <br /> 1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga <br /> 1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca <br /> 1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc <br /> 1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca <br /> 1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa <br /> 2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc <br /> 2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc <br /> 2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg <br /> 2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat <br /> 2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca <br /> 2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt <br /> 2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac <br /> 2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg <br /> 2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg <br /> 2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg <br /> 2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg <br /> 2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga <br /> 2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg <br /> 2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa <br /> 2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt <br /> 2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt <br /> 3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct <br /> 3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac <br /> 3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag <br /> 3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc <br /> 3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc <br /> 3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc <br /> 3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga <br /> 3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt <br /> 3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt <br /> 3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg <br /> 3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc <br /> 3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat <br /> 3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt <br /> 3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc <br /> 3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc <br /> 3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag <br /> 3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt <br /> 4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat <br /> 4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag <br /> 4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac <br /> 4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg <br /> 4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg <br /> 4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt <br /> 4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc <br /> 4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact <br /> 4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg <br /> 4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga <br /> 4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc <br /> 4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt <br /> 4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg <br /> 4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc <br /> 4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag <br /> 4921 aaataatttt gtttaacttt aagaaggaga tataccatgg caagctggag ccacccgcag <br /> 4981 ttcgaaaagg gtgcacttga agtcctcttt cagggacccg ggtaccagga tcctgtacaa <br /> 5041 gtcgacgcgg ccgcagagct cgctctggtg ccacgcggta gttccgctca tcaccaccat <br /> 5101 catcaccatc accaccacta attaacctag gctgctgcca ccgctgagca ataactagca <br /> 5161 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata <br /> 5221 tccggat <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-32_Xa/LIC&diff=328512 pET-32 Xa/LIC 2007-12-13T06:09:28Z <p>Amy: /* Sequence */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET32XALIC.jpg|thumb|250px|right|Image of pET-32 Xa/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB186.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70023-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Trx•Tag&lt;ref name='PMID:15817302'/&gt; coding sequence&lt;br&gt;His•Tag&lt;ref name='PMID:15817302'/&gt; coding sequence&lt;br&gt;S•Tag&lt;ref name='PMID:15817302'/&gt; coding sequence&lt;br&gt;Multiple cloning sites (BseR I - Xho I) &lt;br&gt;His•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla coding sequence&lt;br&gt;f1 origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert. The Srf I and Sma I sites are destroyed during Ligation Independent Cloning.<br /> <br /> ==History ==<br /> The pBAC-2cp Ek/LIC vector is an Ek/LIC version of pBAC-2cp.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB186.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:7763371'/&gt;<br /> &lt;ref name='PMID:15817302'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct <br /> 241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt <br /> 301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat <br /> 361 gatgatggtg catatggcca gaaccagaac cggccaggtt agcgtcgagg aactctttca <br /> 421 actgaccttt agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca <br /> 481 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt <br /> 541 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg <br /> 601 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg <br /> 661 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat <br /> 721 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca <br /> 781 caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta <br /> 841 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat <br /> 901 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt <br /> 961 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca <br /> 1021 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct <br /> 1081 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa <br /> 1141 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga <br /> 1201 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc <br /> 1261 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga <br /> 1321 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt <br /> 1381 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg <br /> 1441 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa <br /> 1501 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc <br /> 1561 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta <br /> 1621 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct <br /> 1681 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa <br /> 1741 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc <br /> 1801 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg <br /> 1861 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga <br /> 1921 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc <br /> 1981 tgcgcgttgg tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt <br /> 2041 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg <br /> 2101 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct <br /> 2161 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt <br /> 2221 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag <br /> 2281 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc <br /> 2341 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc <br /> 2401 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg <br /> 2461 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg <br /> 2521 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt <br /> 2581 ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg <br /> 2641 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa <br /> 2701 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca <br /> 2761 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg <br /> 2821 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca <br /> 2881 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc <br /> 2941 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca <br /> 3001 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac <br /> 3061 agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg <br /> 3121 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg <br /> 3181 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc <br /> 3241 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg <br /> 3301 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg <br /> 3361 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat <br /> 3421 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg <br /> 3481 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc <br /> 3541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa <br /> 3601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa <br /> 3661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct <br /> 3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac <br /> 3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc <br /> 3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc <br /> 3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg <br /> 3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga <br /> 4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag <br /> 4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta <br /> 4141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag <br /> 4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg <br /> 4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac <br /> 4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc <br /> 4381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag <br /> 4441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc <br /> 4501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac <br /> 4561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc <br /> 4621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg <br /> 4681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag <br /> 4741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc <br /> 4801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac <br /> 4861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag <br /> 4921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac <br /> 4981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg <br /> 5041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc <br /> 5101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact <br /> 5161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg <br /> 5221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa <br /> 5281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt <br /> 5341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg <br /> 5401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga <br /> 5461 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt <br /> 5521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat <br /> 5581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa <br /> 5641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta <br /> 5701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc <br /> 5761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc <br /> 5821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac <br /> 5881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]][[Category:His Tag]][[Category:S Tag]][[Category:Trx Tag]][[Category:T7 Promoter]][[Category:Ampicillin Resistant Marker]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-32_Xa/LIC&diff=328511 pET-32 Xa/LIC 2007-12-13T06:02:35Z <p>Amy: /* History */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET32XALIC.jpg|thumb|250px|right|Image of pET-32 Xa/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB186.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70023-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Trx•Tag&lt;ref name='PMID:15817302'/&gt; coding sequence&lt;br&gt;His•Tag&lt;ref name='PMID:15817302'/&gt; coding sequence&lt;br&gt;S•Tag&lt;ref name='PMID:15817302'/&gt; coding sequence&lt;br&gt;Multiple cloning sites (BseR I - Xho I) &lt;br&gt;His•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla coding sequence&lt;br&gt;f1 origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert. The Srf I and Sma I sites are destroyed during Ligation Independent Cloning.<br /> <br /> ==History ==<br /> The pBAC-2cp Ek/LIC vector is an Ek/LIC version of pBAC-2cp.<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB186.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:7763371'/&gt;<br /> &lt;ref name='PMID:15817302'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct <br /> 241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt <br /> 301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat <br /> 361 gatgatggtg catatggcca gaaccagaac cggccaggtt agcgtcgagg aactctttca <br /> 421 actgaccttt agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca <br /> 481 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt <br /> 541 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg <br /> 601 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg <br /> 661 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat <br /> 721 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca <br /> 781 caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta <br /> 841 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat <br /> 901 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt <br /> 961 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca <br /> 1021 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct <br /> 1081 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa <br /> 1141 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga <br /> 1201 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc <br /> 1261 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga <br /> 1321 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt <br /> 1381 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg <br /> 1441 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa <br /> 1501 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc <br /> 1561 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta <br /> 1621 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct <br /> 1681 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa <br /> 1741 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc <br /> 1801 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg <br /> 1861 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga <br /> 1921 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc <br /> 1981 tgcgcgttgg tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt <br /> 2041 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg <br /> 2101 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct <br /> 2161 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt <br /> 2221 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag <br /> 2281 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc <br /> 2341 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc <br /> 2401 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg <br /> 2461 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg <br /> 2521 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt <br /> 2581 ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg <br /> 2641 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa <br /> 2701 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca <br /> 2761 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg <br /> 2821 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca <br /> 2881 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc <br /> 2941 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca <br /> 3001 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac <br /> 3061 agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg <br /> 3121 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg <br /> 3181 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc <br /> 3241 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg <br /> 3301 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg <br /> 3361 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat <br /> 3421 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg <br /> 3481 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc <br /> 3541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa <br /> 3601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa <br /> 3661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct <br /> 3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac <br /> 3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc <br /> 3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc <br /> 3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg <br /> 3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga <br /> 4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag <br /> 4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta <br /> 4141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag <br /> 4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg <br /> 4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac <br /> 4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc <br /> 4381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag <br /> 4441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc <br /> 4501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac <br /> 4561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc <br /> 4621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg <br /> 4681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag <br /> 4741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc <br /> 4801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac <br /> 4861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag <br /> 4921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac <br /> 4981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg <br /> 5041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc <br /> 5101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact <br /> 5161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg <br /> 5221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa <br /> 5281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt <br /> 5341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg <br /> 5401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga <br /> 5461 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt <br /> 5521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat <br /> 5581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa <br /> 5641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta <br /> 5701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc <br /> 5761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc <br /> 5821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac <br /> 5881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-32_Xa/LIC&diff=328510 pET-32 Xa/LIC 2007-12-13T06:01:09Z <p>Amy: edited by Amy via TableEdit</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET32XALIC.jpg|thumb|250px|right|Image of pET-32 Xa/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB186.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70023-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Trx•Tag&lt;ref name='PMID:15817302'/&gt; coding sequence&lt;br&gt;His•Tag&lt;ref name='PMID:15817302'/&gt; coding sequence&lt;br&gt;S•Tag&lt;ref name='PMID:15817302'/&gt; coding sequence&lt;br&gt;Multiple cloning sites (BseR I - Xho I) &lt;br&gt;His•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla coding sequence&lt;br&gt;f1 origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert. The Srf I and Sma I sites are destroyed during Ligation Independent Cloning.<br /> <br /> ==History ==<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB186.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:7763371'/&gt;<br /> &lt;ref name='PMID:15817302'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct <br /> 241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt <br /> 301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat <br /> 361 gatgatggtg catatggcca gaaccagaac cggccaggtt agcgtcgagg aactctttca <br /> 421 actgaccttt agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca <br /> 481 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt <br /> 541 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg <br /> 601 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg <br /> 661 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat <br /> 721 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca <br /> 781 caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta <br /> 841 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat <br /> 901 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt <br /> 961 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca <br /> 1021 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct <br /> 1081 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa <br /> 1141 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga <br /> 1201 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc <br /> 1261 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga <br /> 1321 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt <br /> 1381 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg <br /> 1441 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa <br /> 1501 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc <br /> 1561 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta <br /> 1621 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct <br /> 1681 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa <br /> 1741 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc <br /> 1801 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg <br /> 1861 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga <br /> 1921 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc <br /> 1981 tgcgcgttgg tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt <br /> 2041 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg <br /> 2101 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct <br /> 2161 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt <br /> 2221 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag <br /> 2281 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc <br /> 2341 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc <br /> 2401 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg <br /> 2461 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg <br /> 2521 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt <br /> 2581 ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg <br /> 2641 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa <br /> 2701 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca <br /> 2761 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg <br /> 2821 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca <br /> 2881 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc <br /> 2941 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca <br /> 3001 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac <br /> 3061 agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg <br /> 3121 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg <br /> 3181 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc <br /> 3241 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg <br /> 3301 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg <br /> 3361 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat <br /> 3421 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg <br /> 3481 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc <br /> 3541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa <br /> 3601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa <br /> 3661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct <br /> 3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac <br /> 3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc <br /> 3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc <br /> 3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg <br /> 3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga <br /> 4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag <br /> 4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta <br /> 4141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag <br /> 4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg <br /> 4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac <br /> 4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc <br /> 4381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag <br /> 4441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc <br /> 4501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac <br /> 4561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc <br /> 4621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg <br /> 4681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag <br /> 4741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc <br /> 4801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac <br /> 4861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag <br /> 4921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac <br /> 4981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg <br /> 5041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc <br /> 5101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact <br /> 5161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg <br /> 5221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa <br /> 5281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt <br /> 5341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg <br /> 5401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga <br /> 5461 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt <br /> 5521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat <br /> 5581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa <br /> 5641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta <br /> 5701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc <br /> 5761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc <br /> 5821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac <br /> 5881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-32_Xa/LIC&diff=328509 pET-32 Xa/LIC 2007-12-13T06:00:01Z <p>Amy: /* References */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET32XALIC.jpg|thumb|250px|right|Image of pET-32 Xa/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB186.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70023-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Trx•Tag coding sequence&lt;br&gt;His•Tag coding sequence&lt;br&gt;S•Tag coding sequence&lt;br&gt;Multiple cloning sites (BseR I - Xho I) &lt;br&gt;His•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla coding sequence&lt;br&gt;f1 origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert. The Srf I and Sma I sites are destroyed during Ligation Independent Cloning.<br /> <br /> ==History ==<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB186.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:7763371'/&gt;<br /> &lt;ref name='PMID:15817302'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct <br /> 241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt <br /> 301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat <br /> 361 gatgatggtg catatggcca gaaccagaac cggccaggtt agcgtcgagg aactctttca <br /> 421 actgaccttt agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca <br /> 481 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt <br /> 541 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg <br /> 601 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg <br /> 661 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat <br /> 721 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca <br /> 781 caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta <br /> 841 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat <br /> 901 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt <br /> 961 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca <br /> 1021 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct <br /> 1081 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa <br /> 1141 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga <br /> 1201 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc <br /> 1261 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga <br /> 1321 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt <br /> 1381 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg <br /> 1441 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa <br /> 1501 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc <br /> 1561 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta <br /> 1621 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct <br /> 1681 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa <br /> 1741 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc <br /> 1801 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg <br /> 1861 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga <br /> 1921 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc <br /> 1981 tgcgcgttgg tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt <br /> 2041 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg <br /> 2101 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct <br /> 2161 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt <br /> 2221 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag <br /> 2281 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc <br /> 2341 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc <br /> 2401 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg <br /> 2461 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg <br /> 2521 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt <br /> 2581 ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg <br /> 2641 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa <br /> 2701 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca <br /> 2761 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg <br /> 2821 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca <br /> 2881 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc <br /> 2941 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca <br /> 3001 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac <br /> 3061 agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg <br /> 3121 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg <br /> 3181 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc <br /> 3241 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg <br /> 3301 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg <br /> 3361 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat <br /> 3421 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg <br /> 3481 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc <br /> 3541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa <br /> 3601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa <br /> 3661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct <br /> 3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac <br /> 3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc <br /> 3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc <br /> 3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg <br /> 3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga <br /> 4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag <br /> 4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta <br /> 4141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag <br /> 4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg <br /> 4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac <br /> 4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc <br /> 4381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag <br /> 4441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc <br /> 4501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac <br /> 4561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc <br /> 4621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg <br /> 4681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag <br /> 4741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc <br /> 4801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac <br /> 4861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag <br /> 4921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac <br /> 4981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg <br /> 5041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc <br /> 5101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact <br /> 5161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg <br /> 5221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa <br /> 5281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt <br /> 5341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg <br /> 5401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga <br /> 5461 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt <br /> 5521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat <br /> 5581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa <br /> 5641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta <br /> 5701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc <br /> 5761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc <br /> 5821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac <br /> 5881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-32_Xa/LIC&diff=328508 pET-32 Xa/LIC 2007-12-13T05:55:03Z <p>Amy: /* Notes */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET32XALIC.jpg|thumb|250px|right|Image of pET-32 Xa/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB186.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Ampicillin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70023-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;Trx•Tag coding sequence&lt;br&gt;His•Tag coding sequence&lt;br&gt;S•Tag coding sequence&lt;br&gt;Multiple cloning sites (BseR I - Xho I) &lt;br&gt;His•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;bla coding sequence&lt;br&gt;f1 origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.Z474a63e3dae40--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> <br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert. The Srf I and Sma I sites are destroyed during Ligation Independent Cloning.<br /> <br /> ==History ==<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/docs/docs/PROT/TB186.pdf Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:7763371'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct <br /> 241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt <br /> 301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat <br /> 361 gatgatggtg catatggcca gaaccagaac cggccaggtt agcgtcgagg aactctttca <br /> 421 actgaccttt agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca <br /> 481 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt <br /> 541 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg <br /> 601 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg <br /> 661 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat <br /> 721 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca <br /> 781 caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta <br /> 841 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat <br /> 901 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt <br /> 961 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca <br /> 1021 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct <br /> 1081 aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa <br /> 1141 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga <br /> 1201 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc <br /> 1261 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga <br /> 1321 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt <br /> 1381 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg <br /> 1441 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa <br /> 1501 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc <br /> 1561 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta <br /> 1621 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct <br /> 1681 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa <br /> 1741 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc <br /> 1801 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg <br /> 1861 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga <br /> 1921 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc <br /> 1981 tgcgcgttgg tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt <br /> 2041 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg <br /> 2101 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct <br /> 2161 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt <br /> 2221 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag <br /> 2281 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc <br /> 2341 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc <br /> 2401 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg <br /> 2461 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg <br /> 2521 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt <br /> 2581 ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg <br /> 2641 ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa <br /> 2701 tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca <br /> 2761 acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg <br /> 2821 ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca <br /> 2881 cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc <br /> 2941 cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca <br /> 3001 tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac <br /> 3061 agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg <br /> 3121 gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg <br /> 3181 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc <br /> 3241 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg <br /> 3301 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg <br /> 3361 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat <br /> 3421 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg <br /> 3481 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc <br /> 3541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa <br /> 3601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa <br /> 3661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct <br /> 3721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac <br /> 3781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc <br /> 3841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc <br /> 3901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg <br /> 3961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga <br /> 4021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag <br /> 4081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta <br /> 4141 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag <br /> 4201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg <br /> 4261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac <br /> 4321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc <br /> 4381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag <br /> 4441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc <br /> 4501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac <br /> 4561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc <br /> 4621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg <br /> 4681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag <br /> 4741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc <br /> 4801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac <br /> 4861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag <br /> 4921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac <br /> 4981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg <br /> 5041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc <br /> 5101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact <br /> 5161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg <br /> 5221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa <br /> 5281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt <br /> 5341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg <br /> 5401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga <br /> 5461 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt <br /> 5521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat <br /> 5581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa <br /> 5641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta <br /> 5701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc <br /> 5761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc <br /> 5821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac <br /> 5881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgcca <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-30_Xa/LIC&diff=328507 pET-30 Xa/LIC 2007-12-13T05:47:12Z <p>Amy: /* Notes */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET30XALIC.jpg|thumb|250px|right|Image of pET-30 Xa/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB185.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U474a63ea1e84a--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Kanamycin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70022-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;His•Tag&lt;ref name='PMID:12071693'/&gt; coding sequence&lt;br&gt;S•Tag&lt;ref name='PMID:12071693'/&gt; coding sequence&lt;br&gt;Multiple cloning sites (BseR I - Xho I) &lt;br&gt;His•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;Kan coding sequence&lt;br&gt;f1 origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.U474a63ea1e84a&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U474a63ea1e84a--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions&lt;ref name='PMID:2235490'/&gt;&lt;ref name='PMID:1339364'/&gt;<br /> The LIC method takes advantage of the 3′ → 5′ exonuclease activity of T4 DNA polymerase to create very specific 12 to 15 nucleotide single stranded overhangs in the vector and the insert.<br /> <br /> ==History ==<br /> The pET-30 Xa/LIC vector is an Xa/LIC version of pET-30b(+).<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/product/70073 Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:7763371'/&gt;<br /> &lt;ref name='PMID:12071693'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct <br /> 241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt <br /> 301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat <br /> 361 gatgatggtg catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga <br /> 421 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc <br /> 481 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt <br /> 541 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct <br /> 601 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg <br /> 661 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact <br /> 721 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca <br /> 781 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca <br /> 841 gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt <br /> 901 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa <br /> 961 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg <br /> 1021 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt <br /> 1081 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt <br /> 1141 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc <br /> 1201 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg <br /> 1261 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca <br /> 1321 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat <br /> 1381 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc <br /> 1441 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg <br /> 1501 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca <br /> 1561 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca <br /> 1621 ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt agtgggatac gacgataccg <br /> 1681 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg <br /> 1741 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc <br /> 1801 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg <br /> 1861 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg <br /> 1921 aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccggga <br /> 1981 tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc <br /> 2041 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg <br /> 2101 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc <br /> 2161 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt <br /> 2221 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccccacgg <br /> 2281 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact <br /> 2341 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg <br /> 2401 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa <br /> 2461 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg <br /> 2521 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt <br /> 2581 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac <br /> 2641 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc <br /> 2701 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa <br /> 2761 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc <br /> 2821 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat <br /> 2881 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg <br /> 2941 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt <br /> 3001 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc <br /> 3061 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc <br /> 3121 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg <br /> 3181 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt <br /> 3241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa <br /> 3301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc <br /> 3361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag <br /> 3421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt <br /> 3481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg <br /> 3541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg <br /> 3601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg <br /> 3661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac <br /> 3721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg <br /> 3781 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt <br /> 3841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg <br /> 3901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc <br /> 3961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt <br /> 4021 ggtcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc <br /> 4081 atattcaacg ggaaacgtct tgctctaggc cgcgattaaa ttccaacatg gatgctgatt <br /> 4141 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat <br /> 4201 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca <br /> 4261 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga <br /> 4321 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg <br /> 4381 ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg <br /> 4441 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca <br /> 4501 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg <br /> 4561 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc <br /> 4621 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata <br /> 4681 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg <br /> 4741 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat <br /> 4801 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt <br /> 4861 ttcatttgat gctcgatgag tttttctaag aattaattca tgagcggata catatttgaa <br /> 4921 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct <br /> 4981 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca <br /> 5041 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag <br /> 5101 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc <br /> 5161 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc <br /> 5221 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc <br /> 5281 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa <br /> 5341 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc <br /> 5401 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgcca <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]][[Category:His Tag]][[Category:S Tag]][[Category:Kanamycin Resistant Marker]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-30_Xa/LIC&diff=328506 pET-30 Xa/LIC 2007-12-13T05:41:26Z <p>Amy: edited by Amy via TableEdit</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET30XALIC.jpg|thumb|250px|right|Image of pET-30 Xa/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB185.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U474a63ea1e84a--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Kanamycin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70022-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;His•Tag&lt;ref name='PMID:12071693'/&gt; coding sequence&lt;br&gt;S•Tag&lt;ref name='PMID:12071693'/&gt; coding sequence&lt;br&gt;Multiple cloning sites (BseR I - Xho I) &lt;br&gt;His•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;Kan coding sequence&lt;br&gt;f1 origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.U474a63ea1e84a&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U474a63ea1e84a--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> <br /> ==History ==<br /> The pET-30 Xa/LIC vector is an Xa/LIC version of pET-30b(+).<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/product/70073 Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:7763371'/&gt;<br /> &lt;ref name='PMID:12071693'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct <br /> 241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt <br /> 301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat <br /> 361 gatgatggtg catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga <br /> 421 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc <br /> 481 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt <br /> 541 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct <br /> 601 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg <br /> 661 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact <br /> 721 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca <br /> 781 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca <br /> 841 gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt <br /> 901 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa <br /> 961 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg <br /> 1021 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt <br /> 1081 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt <br /> 1141 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc <br /> 1201 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg <br /> 1261 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca <br /> 1321 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat <br /> 1381 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc <br /> 1441 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg <br /> 1501 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca <br /> 1561 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca <br /> 1621 ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt agtgggatac gacgataccg <br /> 1681 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg <br /> 1741 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc <br /> 1801 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg <br /> 1861 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg <br /> 1921 aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccggga <br /> 1981 tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc <br /> 2041 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg <br /> 2101 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc <br /> 2161 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt <br /> 2221 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccccacgg <br /> 2281 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact <br /> 2341 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg <br /> 2401 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa <br /> 2461 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg <br /> 2521 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt <br /> 2581 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac <br /> 2641 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc <br /> 2701 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa <br /> 2761 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc <br /> 2821 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat <br /> 2881 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg <br /> 2941 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt <br /> 3001 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc <br /> 3061 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc <br /> 3121 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg <br /> 3181 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt <br /> 3241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa <br /> 3301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc <br /> 3361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag <br /> 3421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt <br /> 3481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg <br /> 3541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg <br /> 3601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg <br /> 3661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac <br /> 3721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg <br /> 3781 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt <br /> 3841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg <br /> 3901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc <br /> 3961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt <br /> 4021 ggtcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc <br /> 4081 atattcaacg ggaaacgtct tgctctaggc cgcgattaaa ttccaacatg gatgctgatt <br /> 4141 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat <br /> 4201 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca <br /> 4261 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga <br /> 4321 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg <br /> 4381 ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg <br /> 4441 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca <br /> 4501 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg <br /> 4561 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc <br /> 4621 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata <br /> 4681 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg <br /> 4741 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat <br /> 4801 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt <br /> 4861 ttcatttgat gctcgatgag tttttctaag aattaattca tgagcggata catatttgaa <br /> 4921 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct <br /> 4981 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca <br /> 5041 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag <br /> 5101 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc <br /> 5161 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc <br /> 5221 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc <br /> 5281 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa <br /> 5341 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc <br /> 5401 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgcca <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]][[Category:His Tag]][[Category:S Tag]][[Category:Kanamycin Resistant Marker]]<br /> __NOTOC__</div> Amy https://ecoliwiki.org/colipedia/index.php?title=pET-30_Xa/LIC&diff=328505 pET-30 Xa/LIC 2007-12-13T05:40:05Z <p>Amy: /* References */</p> <hr /> <div>==At a Glance ==<br /> [[Image:PET30XALIC.jpg|thumb|250px|right|Image of pET-30 Xa/LIC. [http://www.emdbiosciences.com/docs/docs/PROT/TB185.pdf Novagen]]]<br /> &lt;protect&gt;&lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U474a63ea1e84a--&gt;<br /> &lt;!--<br /> ******************************************************************************************<br /> *<br /> * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. **<br /> *<br /> ****************************************************************************************** --&gt;<br /> {|{{Prettytable}} width='30%' align=left<br /> |-<br /> !{{table heading style}} |Type<br /> |<br /> Cloning<br /> |-<br /> !{{table heading style}} |Origin of Replication<br /> |<br /> f1<br /> |-<br /> !{{table heading style}} |Copy #<br /> |<br /> ~40<br /> |-<br /> !{{table heading style}} |Markers<br /> |<br /> Kanamycin<br /> |-<br /> !{{table heading style}} |Link to Sequence<br /> |<br /> [http://www.emdbiosciences.com/docs/NDIS/70022-000.html Novagen]<br /> |-<br /> !{{table heading style}} |Features<br /> |<br /> T7 promoter&lt;br&gt;T7 transcription start&lt;br&gt;His•Tag coding sequence&lt;br&gt;S•Tag coding sequence&lt;br&gt;Multiple cloning sites (BseR I - Xho I) &lt;br&gt;His•Tag coding sequence&lt;br&gt;T7 terminator&lt;br&gt;lacI coding sequence&lt;br&gt;pBR322 origin&lt;br&gt;Kan coding sequence&lt;br&gt;f1 origin<br /> |-<br /> !{{table heading style}} |Accession Number<br /> |<br /> <br /> |-<br /> |colspan='2'|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&amp;id=63ce1649aab8b3180235b0af7618f667.0.U474a63ea1e84a&amp;page=0&amp;pagename={{FULLPAGENAMEE}} edit table]<br /> |}<br /> &lt;!--box uid=63ce1649aab8b3180235b0af7618f667.0.U474a63ea1e84a--&gt;&lt;/protect&gt;<br /> &lt;BR&gt;<br /> &lt;Br clear='all'&gt;<br /> ==Notes==<br /> <br /> ==History ==<br /> The pET-30 Xa/LIC vector is an Xa/LIC version of pET-30b(+).<br /> <br /> ==Sources ==<br /> [http://www.emdbiosciences.com/product/70073 Novagen]<br /> <br /> ==References==<br /> &lt;ref name='PMID:7763371'/&gt;<br /> &lt;ref name='PMID:12071693'/&gt;<br /> &lt;references/&gt;<br /> <br /> ==Sequence ==<br /> 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa <br /> 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt <br /> 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt <br /> 181 cgacggagct cgaattcgga tccgatatcg ccatggccag aggagagtta gagcgaccct <br /> 241 caataccgga gccaccaccg gtacccagat ctgggctgtc catgtgctgg cgttcgaatt <br /> 301 tagcagcagc ggtttctttc ataccagaac cgcgtggcac cagaccagaa gaatgatgat <br /> 361 gatgatggtg catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga <br /> 421 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc <br /> 481 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt <br /> 541 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct <br /> 601 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg <br /> 661 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact <br /> 721 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca <br /> 781 tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca <br /> 841 gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt <br /> 901 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa <br /> 961 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg <br /> 1021 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt <br /> 1081 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt <br /> 1141 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc <br /> 1201 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg <br /> 1261 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca <br /> 1321 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat <br /> 1381 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc <br /> 1441 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg <br /> 1501 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca <br /> 1561 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca <br /> 1621 ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt agtgggatac gacgataccg <br /> 1681 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg <br /> 1741 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc <br /> 1801 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg <br /> 1861 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg <br /> 1921 aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccggga <br /> 1981 tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc <br /> 2041 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg <br /> 2101 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc <br /> 2161 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt <br /> 2221 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccccacgg <br /> 2281 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact <br /> 2341 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg <br /> 2401 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa <br /> 2461 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg <br /> 2521 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt <br /> 2581 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac <br /> 2641 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc <br /> 2701 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa <br /> 2761 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc <br /> 2821 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat <br /> 2881 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg <br /> 2941 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt <br /> 3001 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc <br /> 3061 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc <br /> 3121 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg <br /> 3181 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt <br /> 3241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa <br /> 3301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc <br /> 3361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag <br /> 3421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt <br /> 3481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg <br /> 3541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg <br /> 3601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg <br /> 3661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac <br /> 3721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg <br /> 3781 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt <br /> 3841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg <br /> 3901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc <br /> 3961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt <br /> 4021 ggtcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc <br /> 4081 atattcaacg ggaaacgtct tgctctaggc cgcgattaaa ttccaacatg gatgctgatt <br /> 4141 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat <br /> 4201 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca <br /> 4261 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga <br /> 4321 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg <br /> 4381 ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg <br /> 4441 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca <br /> 4501 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg <br /> 4561 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc <br /> 4621 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata <br /> 4681 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg <br /> 4741 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat <br /> 4801 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt <br /> 4861 ttcatttgat gctcgatgag tttttctaag aattaattca tgagcggata catatttgaa <br /> 4921 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct <br /> 4981 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca <br /> 5041 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag <br /> 5101 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc <br /> 5161 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc <br /> 5221 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc <br /> 5281 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa <br /> 5341 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc <br /> 5401 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgcca <br /> <br /> <br /> <br /> <br /> [[Category:Plasmids]][[Category:Cloning Plasmids]][[Category:Novagen Plasmids]][[Category:His Tag]][[Category:S Tag]][[Category:Kanamycin Resistant Marker]]<br /> __NOTOC__</div> Amy