EcoliWiki - User contributions [en]

Addgene

2014-07-22T14:07:26Z

Addgene: /* Searching */

{{Pagetop}}
[[File:Addgene New Logo.jpg|center]]
<protect>

{| {{Prettytable}} width='30%' align="right" id="J48049604a1b31" class=" tableEdit Database_table"

|-
!align=left {{table heading style}} |Link/URL:
||
[http://www.addgene.org/vector-database/ www.Addgene.org]
|-
!align=left {{table heading style}} |What:
||
Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) [http://www.addgene.org/vector-database/query/?q_vdb=#searchbar Click to search vector database now].
|-
!align=left {{table heading style}} |Who:
||
Addgene is a nonprofit global plasmid repository dedicated to helping scientists share their plasmids.
|-
!align=left {{table heading style}} |Updates:
||
Do you use plasmids?

Join the non-profit plasmid sharing community. Visit [http://www.addgene.org/ www.addgene.org] to learn more, find plasmids, and start depositing!
|-
!align=left {{table heading style}} |Upcoming events:
||
Visit [http://www.addgene.org/news/ Addgene's News and Announcements page] to see current news and announcements.
|-
!align=left {{table heading style}} |Web Services:
||
[http://www.addgene.org/ Addgene] is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J48049604a1b31&page=133952&pagename={{FULLPAGENAMEE}}&type=1&template=Database_table edit table] ||
|}
</protect>
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.

==Content{{HelpIcon|Help:Databases}}==
'''Plasmid Repository'''

As of 2014, Addgene's repository stores and distributes over 30,000 plasmids, contributed by over 2,000 depositing labs. Addgene's collection contains plasmids used for a variety of functions such as genome engineering (including CRISPR systems), gene expression, shRNA knockdown, viral-mediated gene deliveray, detection of miRNA, and promoter activity.

The plasmid collection includes:

* Genome engineering
** [http://www.addgene.org/CRISPR/ CRISPR]s
** [http://www.addgene.org/TALEN/guide/ Transcription Activator-Like Effector Nuclease] (TALEN) kits
** [http://www.addgene.org/zfc/ Zinc finger nuclease] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [http://www.addgene.org/fluorescent_proteins/ Fluorescent marker]
* Viral vectors
** [http://www.addgene.org/lentiviral/ Retroviral/Lentiviral]
** Adenoviral
** Adeno-associated virus
* cDNA expression
* shRNA expression

==Tools and guides==

Addgene's educational plasmid resource include guides, tools, and protocols created by Addgene and depositing labs to help scientists with their molecular biology experiments.

'''Molecular Biology Tools'''

[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

'''Plasmid Cloning Guides'''

[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==

Addgene's collection of plasmids has been assembled from research labs all over the world. If your lab receives requests for plasmids, consider depositing them to Addgene (learn more about depositing to Addgene).

===Browsing===

Addgene's plasmid collection is easily navigated through search criteria. Popular searches include plasmid type, source popularity, selectable marker, bacterial resistance, and bacterial strain. Browse Plasmid Repository by plasmid function or type [http://www.addgene.org/vector-database/ Browse Plasmid Repository].

<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C51e44c9b03c66" class=" tableEdit "
|-
!|Browse By Plasmid Type:!!
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mammalian Mammalian Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Bacterial Bacterial Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Luciferase Luciferase]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mouse Mouse Targeting]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Retroviral Retroviral]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Lentiviral Lentiviral]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Worm Worm Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Insect Insect Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Yeast Yeast Expression, E.Coli or yeast]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:RNAi RNAi]
|-
|
[http://www.addgene.org/search/advanced/?q=&depositor=&article=&gene=&vector=&tags=&advanced_query=&results_per_page=20&page=1&selected_facets=plasmid_type_exact%3A7&sort_type=relevance Bacterial Strain]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.C51e44c9b03c66&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

===Searching===

Search Addgene's collection by plasmid source, slectable marker, or bacterial resistance.

<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P51e44b532cef6" class=" tableEdit "
|-
!|Additional Searches By:!!
|-
|
Bacterial Resistance
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Ampicillin Ampicillin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Kanamycin Kanamycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Tetracycline Tetracycline]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Chloramphenicol Chloramphenicol]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Spectinomycin Spectinomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Gentamycin Gentamycin]
|-
|
Selectable Marker
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Neomycin Neomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Puromycin Puromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Zeocin Zeocin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:URA3 URA3]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:LEU2 LEU2]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:TRP1 TRP1];[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:HIS3 HIS3]
|-
|
Source
|
[http://www.addgene.org/vector-database/query/?q_vdb=source:addgene Addgene]; [http://www.addgene.org/vector-database/query/?q_vdb=source:ATCC ATCC]; [http://www.addgene.org/vector-database/query/?q_vdb=source:BD%20%20Biosciences BD Biosciences]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Invitrogen Invitrogen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Novagen Novagen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Sigma Sigma]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Stratagene Stratagene]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.P51e44b532cef6&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

Addgene

2014-07-22T14:05:48Z

Addgene: Table edited by Addgene via TableEdit

{{Pagetop}}
[[File:Addgene New Logo.jpg|center]]
<protect>

{| {{Prettytable}} width='30%' align="right" id="J48049604a1b31" class=" tableEdit Database_table"

|-
!align=left {{table heading style}} |Link/URL:
||
[http://www.addgene.org/vector-database/ www.Addgene.org]
|-
!align=left {{table heading style}} |What:
||
Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) [http://www.addgene.org/vector-database/query/?q_vdb=#searchbar Click to search vector database now].
|-
!align=left {{table heading style}} |Who:
||
Addgene is a nonprofit global plasmid repository dedicated to helping scientists share their plasmids.
|-
!align=left {{table heading style}} |Updates:
||
Do you use plasmids?

Join the non-profit plasmid sharing community. Visit [http://www.addgene.org/ www.addgene.org] to learn more, find plasmids, and start depositing!
|-
!align=left {{table heading style}} |Upcoming events:
||
Visit [http://www.addgene.org/news/ Addgene's News and Announcements page] to see current news and announcements.
|-
!align=left {{table heading style}} |Web Services:
||
[http://www.addgene.org/ Addgene] is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J48049604a1b31&page=133952&pagename={{FULLPAGENAMEE}}&type=1&template=Database_table edit table] ||
|}
</protect>
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.

==Content{{HelpIcon|Help:Databases}}==
'''Plasmid Repository'''

As of 2014, Addgene's repository stores and distributes over 30,000 plasmids, contributed by over 2,000 depositing labs. Addgene's collection contains plasmids used for a variety of functions such as genome engineering (including CRISPR systems), gene expression, shRNA knockdown, viral-mediated gene deliveray, detection of miRNA, and promoter activity.

The plasmid collection includes:

* Genome engineering
** [http://www.addgene.org/CRISPR/ CRISPR]s
** [http://www.addgene.org/TALEN/guide/ Transcription Activator-Like Effector Nuclease] (TALEN) kits
** [http://www.addgene.org/zfc/ Zinc finger nuclease] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [http://www.addgene.org/fluorescent_proteins/ Fluorescent marker]
* Viral vectors
** [http://www.addgene.org/lentiviral/ Retroviral/Lentiviral]
** Adenoviral
** Adeno-associated virus
* cDNA expression
* shRNA expression

==Tools and guides==

Addgene's educational plasmid resource include guides, tools, and protocols created by Addgene and depositing labs to help scientists with their molecular biology experiments.

'''Molecular Biology Tools'''

[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

'''Plasmid Cloning Guides'''

[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==

Addgene's collection of plasmids has been assembled from research labs all over the world. If your lab receives requests for plasmids, consider depositing them to Addgene (learn more about depositing to Addgene).

===Browsing===

Addgene's plasmid collection is easily navigated through search criteria. Popular searches include plasmid type, source popularity, selectable marker, bacterial resistance, and bacterial strain. Browse Plasmid Repository by plasmid function or type [http://www.addgene.org/vector-database/ Browse Plasmid Repository].

<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C51e44c9b03c66" class=" tableEdit "
|-
!|Browse By Plasmid Type:!!
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mammalian Mammalian Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Bacterial Bacterial Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Luciferase Luciferase]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mouse Mouse Targeting]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Retroviral Retroviral]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Lentiviral Lentiviral]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Worm Worm Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Insect Insect Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Yeast Yeast Expression, E.Coli or yeast]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:RNAi RNAi]
|-
|
[http://www.addgene.org/search/advanced/?q=&depositor=&article=&gene=&vector=&tags=&advanced_query=&results_per_page=20&page=1&selected_facets=plasmid_type_exact%3A7&sort_type=relevance Bacterial Strain]
|

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.C51e44c9b03c66&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

===Searching===
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P51e44b532cef6" class=" tableEdit "
|-
!|Additional Searches By:!!
|-
|
Bacterial Resistance
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Ampicillin Ampicillin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Kanamycin Kanamycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Tetracycline Tetracycline]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Chloramphenicol Chloramphenicol]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Spectinomycin Spectinomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Gentamycin Gentamycin]
|-
|
Selectable Marker
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Neomycin Neomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Puromycin Puromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Zeocin Zeocin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:URA3 URA3]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:LEU2 LEU2]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:TRP1 TRP1];[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:HIS3 HIS3]
|-
|
Source
|
[http://www.addgene.org/vector-database/query/?q_vdb=source:addgene Addgene]; [http://www.addgene.org/vector-database/query/?q_vdb=source:ATCC ATCC]; [http://www.addgene.org/vector-database/query/?q_vdb=source:BD%20%20Biosciences BD Biosciences]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Invitrogen Invitrogen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Novagen Novagen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Sigma Sigma]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Stratagene Stratagene]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.P51e44b532cef6&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

Addgene

2014-07-22T14:01:42Z

Addgene: /* Browsing */

{{Pagetop}}
[[File:Addgene New Logo.jpg|center]]
<protect>

{| {{Prettytable}} width='30%' align="right" id="J48049604a1b31" class=" tableEdit Database_table"

|-
!align=left {{table heading style}} |Link/URL:
||
[http://www.addgene.org/vector-database/ www.Addgene.org]
|-
!align=left {{table heading style}} |What:
||
Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) [http://www.addgene.org/vector-database/query/?q_vdb=#searchbar Click to search vector database now].
|-
!align=left {{table heading style}} |Who:
||
Addgene is a nonprofit global plasmid repository dedicated to helping scientists share their plasmids.
|-
!align=left {{table heading style}} |Updates:
||
Do you use plasmids?

Join the non-profit plasmid sharing community. Visit [http://www.addgene.org/ www.addgene.org] to learn more, find plasmids, and start depositing!
|-
!align=left {{table heading style}} |Upcoming events:
||
Visit [http://www.addgene.org/news/ Addgene's News and Announcements page] to see current news and announcements.
|-
!align=left {{table heading style}} |Web Services:
||
[http://www.addgene.org/ Addgene] is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J48049604a1b31&page=133952&pagename={{FULLPAGENAMEE}}&type=1&template=Database_table edit table] ||
|}
</protect>
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.

==Content{{HelpIcon|Help:Databases}}==
'''Plasmid Repository'''

As of 2014, Addgene's repository stores and distributes over 30,000 plasmids, contributed by over 2,000 depositing labs. Addgene's collection contains plasmids used for a variety of functions such as genome engineering (including CRISPR systems), gene expression, shRNA knockdown, viral-mediated gene deliveray, detection of miRNA, and promoter activity.

The plasmid collection includes:

* Genome engineering
** [http://www.addgene.org/CRISPR/ CRISPR]s
** [http://www.addgene.org/TALEN/guide/ Transcription Activator-Like Effector Nuclease] (TALEN) kits
** [http://www.addgene.org/zfc/ Zinc finger nuclease] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [http://www.addgene.org/fluorescent_proteins/ Fluorescent marker]
* Viral vectors
** [http://www.addgene.org/lentiviral/ Retroviral/Lentiviral]
** Adenoviral
** Adeno-associated virus
* cDNA expression
* shRNA expression

==Tools and guides==

Addgene's educational plasmid resource include guides, tools, and protocols created by Addgene and depositing labs to help scientists with their molecular biology experiments.

'''Molecular Biology Tools'''

[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

'''Plasmid Cloning Guides'''

[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==

Addgene's collection of plasmids has been assembled from research labs all over the world. If your lab receives requests for plasmids, consider depositing them to Addgene (learn more about depositing to Addgene).

===Browsing===

Addgene's plasmid collection is easily navigated through search criteria. Popular searches include plasmid type, source popularity, selectable marker, bacterial resistance, and bacterial strain. Browse Plasmid Repository by plasmid function or type [http://www.addgene.org/vector-database/ Browse Plasmid Repository].

<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C51e44c9b03c66" class=" tableEdit "
|-
!|Browse By Plasmid Type:!!
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mammalian Mammalian Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Bacterial Bacterial Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Luciferase Luciferase]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mouse Mouse Targeting]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Retroviral Retroviral]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Lentiviral Lentiviral]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Worm Worm Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Insect Insect Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Yeast Yeast Expression, E.Coli or yeast]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:RNAi RNAi]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.C51e44c9b03c66&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

===Searching===
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P51e44b532cef6" class=" tableEdit "
|-
!|Additional Searches By:!!
|-
|
Bacterial Resistance
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Ampicillin Ampicillin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Kanamycin Kanamycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Tetracycline Tetracycline]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Chloramphenicol Chloramphenicol]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Spectinomycin Spectinomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Gentamycin Gentamycin]
|-
|
Selectable Marker
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Neomycin Neomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Puromycin Puromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Zeocin Zeocin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:URA3 URA3]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:LEU2 LEU2]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:TRP1 TRP1];[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:HIS3 HIS3]
|-
|
Source
|
[http://www.addgene.org/vector-database/query/?q_vdb=source:addgene Addgene]; [http://www.addgene.org/vector-database/query/?q_vdb=source:ATCC ATCC]; [http://www.addgene.org/vector-database/query/?q_vdb=source:BD%20%20Biosciences BD Biosciences]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Invitrogen Invitrogen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Novagen Novagen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Sigma Sigma]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Stratagene Stratagene]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.P51e44b532cef6&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

Addgene

2014-07-22T14:00:20Z

Addgene: /* Browsing */

{{Pagetop}}
[[File:Addgene New Logo.jpg|center]]
<protect>

{| {{Prettytable}} width='30%' align="right" id="J48049604a1b31" class=" tableEdit Database_table"

|-
!align=left {{table heading style}} |Link/URL:
||
[http://www.addgene.org/vector-database/ www.Addgene.org]
|-
!align=left {{table heading style}} |What:
||
Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) [http://www.addgene.org/vector-database/query/?q_vdb=#searchbar Click to search vector database now].
|-
!align=left {{table heading style}} |Who:
||
Addgene is a nonprofit global plasmid repository dedicated to helping scientists share their plasmids.
|-
!align=left {{table heading style}} |Updates:
||
Do you use plasmids?

Join the non-profit plasmid sharing community. Visit [http://www.addgene.org/ www.addgene.org] to learn more, find plasmids, and start depositing!
|-
!align=left {{table heading style}} |Upcoming events:
||
Visit [http://www.addgene.org/news/ Addgene's News and Announcements page] to see current news and announcements.
|-
!align=left {{table heading style}} |Web Services:
||
[http://www.addgene.org/ Addgene] is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J48049604a1b31&page=133952&pagename={{FULLPAGENAMEE}}&type=1&template=Database_table edit table] ||
|}
</protect>
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.

==Content{{HelpIcon|Help:Databases}}==
'''Plasmid Repository'''

As of 2014, Addgene's repository stores and distributes over 30,000 plasmids, contributed by over 2,000 depositing labs. Addgene's collection contains plasmids used for a variety of functions such as genome engineering (including CRISPR systems), gene expression, shRNA knockdown, viral-mediated gene deliveray, detection of miRNA, and promoter activity.

The plasmid collection includes:

* Genome engineering
** [http://www.addgene.org/CRISPR/ CRISPR]s
** [http://www.addgene.org/TALEN/guide/ Transcription Activator-Like Effector Nuclease] (TALEN) kits
** [http://www.addgene.org/zfc/ Zinc finger nuclease] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [http://www.addgene.org/fluorescent_proteins/ Fluorescent marker]
* Viral vectors
** [http://www.addgene.org/lentiviral/ Retroviral/Lentiviral]
** Adenoviral
** Adeno-associated virus
* cDNA expression
* shRNA expression

==Tools and guides==

Addgene's educational plasmid resource include guides, tools, and protocols created by Addgene and depositing labs to help scientists with their molecular biology experiments.

'''Molecular Biology Tools'''

[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

'''Plasmid Cloning Guides'''

[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==

Addgene's collection of plasmids has been assembled from research labs all over the world. If your lab receives requests for plasmids, consider depositing them to Addgene (learn more about depositing to Addgene).

===Browsing===

Addgene's plasmid collection is easily navigated through search criteria. Popular searches include plasmid type, source popularity, selectable marker, bacterial resistance, and bacterial strain. [http://www.addgene.org/vector-database/ Browse Plasmid Repository] by plasmid type, source, bacterial resistance, and selectable marker.

<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C51e44c9b03c66" class=" tableEdit "
|-
!|Browse By Plasmid Type:!!
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mammalian Mammalian Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Bacterial Bacterial Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Luciferase Luciferase]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mouse Mouse Targeting]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Retroviral Retroviral]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Lentiviral Lentiviral]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Worm Worm Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Insect Insect Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Yeast Yeast Expression, E.Coli or yeast]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:RNAi RNAi]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.C51e44c9b03c66&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

===Searching===
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P51e44b532cef6" class=" tableEdit "
|-
!|Additional Searches By:!!
|-
|
Bacterial Resistance
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Ampicillin Ampicillin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Kanamycin Kanamycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Tetracycline Tetracycline]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Chloramphenicol Chloramphenicol]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Spectinomycin Spectinomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Gentamycin Gentamycin]
|-
|
Selectable Marker
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Neomycin Neomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Puromycin Puromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Zeocin Zeocin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:URA3 URA3]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:LEU2 LEU2]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:TRP1 TRP1];[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:HIS3 HIS3]
|-
|
Source
|
[http://www.addgene.org/vector-database/query/?q_vdb=source:addgene Addgene]; [http://www.addgene.org/vector-database/query/?q_vdb=source:ATCC ATCC]; [http://www.addgene.org/vector-database/query/?q_vdb=source:BD%20%20Biosciences BD Biosciences]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Invitrogen Invitrogen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Novagen Novagen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Sigma Sigma]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Stratagene Stratagene]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.P51e44b532cef6&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

Addgene

2014-07-22T13:59:39Z

Addgene: /* Browsing */

{{Pagetop}}
[[File:Addgene New Logo.jpg|center]]
<protect>

{| {{Prettytable}} width='30%' align="right" id="J48049604a1b31" class=" tableEdit Database_table"

|-
!align=left {{table heading style}} |Link/URL:
||
[http://www.addgene.org/vector-database/ www.Addgene.org]
|-
!align=left {{table heading style}} |What:
||
Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) [http://www.addgene.org/vector-database/query/?q_vdb=#searchbar Click to search vector database now].
|-
!align=left {{table heading style}} |Who:
||
Addgene is a nonprofit global plasmid repository dedicated to helping scientists share their plasmids.
|-
!align=left {{table heading style}} |Updates:
||
Do you use plasmids?

Join the non-profit plasmid sharing community. Visit [http://www.addgene.org/ www.addgene.org] to learn more, find plasmids, and start depositing!
|-
!align=left {{table heading style}} |Upcoming events:
||
Visit [http://www.addgene.org/news/ Addgene's News and Announcements page] to see current news and announcements.
|-
!align=left {{table heading style}} |Web Services:
||
[http://www.addgene.org/ Addgene] is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J48049604a1b31&page=133952&pagename={{FULLPAGENAMEE}}&type=1&template=Database_table edit table] ||
|}
</protect>
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.

==Content{{HelpIcon|Help:Databases}}==
'''Plasmid Repository'''

As of 2014, Addgene's repository stores and distributes over 30,000 plasmids, contributed by over 2,000 depositing labs. Addgene's collection contains plasmids used for a variety of functions such as genome engineering (including CRISPR systems), gene expression, shRNA knockdown, viral-mediated gene deliveray, detection of miRNA, and promoter activity.

The plasmid collection includes:

* Genome engineering
** [http://www.addgene.org/CRISPR/ CRISPR]s
** [http://www.addgene.org/TALEN/guide/ Transcription Activator-Like Effector Nuclease] (TALEN) kits
** [http://www.addgene.org/zfc/ Zinc finger nuclease] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [http://www.addgene.org/fluorescent_proteins/ Fluorescent marker]
* Viral vectors
** [http://www.addgene.org/lentiviral/ Retroviral/Lentiviral]
** Adenoviral
** Adeno-associated virus
* cDNA expression
* shRNA expression

==Tools and guides==

Addgene's educational plasmid resource include guides, tools, and protocols created by Addgene and depositing labs to help scientists with their molecular biology experiments.

'''Molecular Biology Tools'''

[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

'''Plasmid Cloning Guides'''

[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==

Addgene's collection of plasmids has been assembled from research labs all over the world. If your lab receives requests for plasmids, consider depositing them to Addgene (learn more about depositing to Addgene).

===Browsing===

Addgene's plasmid collection is easily navigated through search criteria. Popular searches include plasmid type, source popularity, selectable marker, bacterial resistance, and bacterial strain. [http://www.addgene.org/vector-database/ Browse Plasmid Repository] by plasmid type, source, bacterial resistance, and selectable marker.

<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C51e44c9b03c66" class=" tableEdit "
|-
!|Browse By Plasmid Type:!!
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mammalian Mammalian Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Bacterial Bacterial Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Luciferase Luciferase]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mouse Mouse Targeting]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Retroviral Retroviral]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Lentiviral Lentiviral]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Worm Worm Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Insect Insect Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Yeast Yeast Expression, E.Coli or yeast]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:RNAi RNAi]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.C51e44c9b03c66&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

===Searching===
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P51e44b532cef6" class=" tableEdit "
|-
!|Additional Searches By:!!
|-
|
Bacterial Resistance
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Ampicillin Ampicillin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Kanamycin Kanamycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Tetracycline Tetracycline]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Chloramphenicol Chloramphenicol]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Spectinomycin Spectinomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Gentamycin Gentamycin]
|-
|
Selectable Marker
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Neomycin Neomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Puromycin Puromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Zeocin Zeocin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:URA3 URA3]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:LEU2 LEU2]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:TRP1 TRP1];[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:HIS3 HIS3]
|-
|
Source
|
[http://www.addgene.org/vector-database/query/?q_vdb=source:addgene Addgene]; [http://www.addgene.org/vector-database/query/?q_vdb=source:ATCC ATCC]; [http://www.addgene.org/vector-database/query/?q_vdb=source:BD%20%20Biosciences BD Biosciences]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Invitrogen Invitrogen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Novagen Novagen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Sigma Sigma]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Stratagene Stratagene]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.P51e44b532cef6&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

Addgene

2014-07-22T13:58:55Z

Addgene: /* Using {{PAGENAME}} {{HelpIcon|Help:Databases}} */

{{Pagetop}}
[[File:Addgene New Logo.jpg|center]]
<protect>

{| {{Prettytable}} width='30%' align="right" id="J48049604a1b31" class=" tableEdit Database_table"

|-
!align=left {{table heading style}} |Link/URL:
||
[http://www.addgene.org/vector-database/ www.Addgene.org]
|-
!align=left {{table heading style}} |What:
||
Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) [http://www.addgene.org/vector-database/query/?q_vdb=#searchbar Click to search vector database now].
|-
!align=left {{table heading style}} |Who:
||
Addgene is a nonprofit global plasmid repository dedicated to helping scientists share their plasmids.
|-
!align=left {{table heading style}} |Updates:
||
Do you use plasmids?

Join the non-profit plasmid sharing community. Visit [http://www.addgene.org/ www.addgene.org] to learn more, find plasmids, and start depositing!
|-
!align=left {{table heading style}} |Upcoming events:
||
Visit [http://www.addgene.org/news/ Addgene's News and Announcements page] to see current news and announcements.
|-
!align=left {{table heading style}} |Web Services:
||
[http://www.addgene.org/ Addgene] is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J48049604a1b31&page=133952&pagename={{FULLPAGENAMEE}}&type=1&template=Database_table edit table] ||
|}
</protect>
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.

==Content{{HelpIcon|Help:Databases}}==
'''Plasmid Repository'''

As of 2014, Addgene's repository stores and distributes over 30,000 plasmids, contributed by over 2,000 depositing labs. Addgene's collection contains plasmids used for a variety of functions such as genome engineering (including CRISPR systems), gene expression, shRNA knockdown, viral-mediated gene deliveray, detection of miRNA, and promoter activity.

The plasmid collection includes:

* Genome engineering
** [http://www.addgene.org/CRISPR/ CRISPR]s
** [http://www.addgene.org/TALEN/guide/ Transcription Activator-Like Effector Nuclease] (TALEN) kits
** [http://www.addgene.org/zfc/ Zinc finger nuclease] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [http://www.addgene.org/fluorescent_proteins/ Fluorescent marker]
* Viral vectors
** [http://www.addgene.org/lentiviral/ Retroviral/Lentiviral]
** Adenoviral
** Adeno-associated virus
* cDNA expression
* shRNA expression

==Tools and guides==

Addgene's educational plasmid resource include guides, tools, and protocols created by Addgene and depositing labs to help scientists with their molecular biology experiments.

'''Molecular Biology Tools'''

[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

'''Plasmid Cloning Guides'''

[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==

Addgene's collection of plasmids has been assembled from research labs all over the world. If your lab receives requests for plasmids, consider depositing them to Addgene (learn more about depositing to Addgene).

===Browsing===
[http://www.addgene.org/vector-database/ Browse Plasmid Repository] by plasmid type, source, bacterial resistance, and selectable marker.

<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C51e44c9b03c66" class=" tableEdit "
|-
!|Browse By Plasmid Type:!!
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mammalian Mammalian Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Bacterial Bacterial Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Luciferase Luciferase]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mouse Mouse Targeting]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Retroviral Retroviral]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Lentiviral Lentiviral]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Worm Worm Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Insect Insect Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Yeast Yeast Expression, E.Coli or yeast]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:RNAi RNAi]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.C51e44c9b03c66&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

===Searching===
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P51e44b532cef6" class=" tableEdit "
|-
!|Additional Searches By:!!
|-
|
Bacterial Resistance
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Ampicillin Ampicillin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Kanamycin Kanamycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Tetracycline Tetracycline]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Chloramphenicol Chloramphenicol]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Spectinomycin Spectinomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Gentamycin Gentamycin]
|-
|
Selectable Marker
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Neomycin Neomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Puromycin Puromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Zeocin Zeocin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:URA3 URA3]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:LEU2 LEU2]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:TRP1 TRP1];[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:HIS3 HIS3]
|-
|
Source
|
[http://www.addgene.org/vector-database/query/?q_vdb=source:addgene Addgene]; [http://www.addgene.org/vector-database/query/?q_vdb=source:ATCC ATCC]; [http://www.addgene.org/vector-database/query/?q_vdb=source:BD%20%20Biosciences BD Biosciences]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Invitrogen Invitrogen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Novagen Novagen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Sigma Sigma]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Stratagene Stratagene]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.P51e44b532cef6&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

Addgene

2014-07-22T13:57:43Z

Addgene: /* Tools and guides */

{{Pagetop}}
[[File:Addgene New Logo.jpg|center]]
<protect>

{| {{Prettytable}} width='30%' align="right" id="J48049604a1b31" class=" tableEdit Database_table"

|-
!align=left {{table heading style}} |Link/URL:
||
[http://www.addgene.org/vector-database/ www.Addgene.org]
|-
!align=left {{table heading style}} |What:
||
Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) [http://www.addgene.org/vector-database/query/?q_vdb=#searchbar Click to search vector database now].
|-
!align=left {{table heading style}} |Who:
||
Addgene is a nonprofit global plasmid repository dedicated to helping scientists share their plasmids.
|-
!align=left {{table heading style}} |Updates:
||
Do you use plasmids?

Join the non-profit plasmid sharing community. Visit [http://www.addgene.org/ www.addgene.org] to learn more, find plasmids, and start depositing!
|-
!align=left {{table heading style}} |Upcoming events:
||
Visit [http://www.addgene.org/news/ Addgene's News and Announcements page] to see current news and announcements.
|-
!align=left {{table heading style}} |Web Services:
||
[http://www.addgene.org/ Addgene] is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J48049604a1b31&page=133952&pagename={{FULLPAGENAMEE}}&type=1&template=Database_table edit table] ||
|}
</protect>
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.

==Content{{HelpIcon|Help:Databases}}==
'''Plasmid Repository'''

As of 2014, Addgene's repository stores and distributes over 30,000 plasmids, contributed by over 2,000 depositing labs. Addgene's collection contains plasmids used for a variety of functions such as genome engineering (including CRISPR systems), gene expression, shRNA knockdown, viral-mediated gene deliveray, detection of miRNA, and promoter activity.

The plasmid collection includes:

* Genome engineering
** [http://www.addgene.org/CRISPR/ CRISPR]s
** [http://www.addgene.org/TALEN/guide/ Transcription Activator-Like Effector Nuclease] (TALEN) kits
** [http://www.addgene.org/zfc/ Zinc finger nuclease] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [http://www.addgene.org/fluorescent_proteins/ Fluorescent marker]
* Viral vectors
** [http://www.addgene.org/lentiviral/ Retroviral/Lentiviral]
** Adenoviral
** Adeno-associated virus
* cDNA expression
* shRNA expression

==Tools and guides==

Addgene's educational plasmid resource include guides, tools, and protocols created by Addgene and depositing labs to help scientists with their molecular biology experiments.

'''Molecular Biology Tools'''

[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

'''Plasmid Cloning Guides'''

[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==
===Browsing===
[http://www.addgene.org/vector-database/ Browse Plasmid Repository] by plasmid type, source, bacterial resistance, and selectable marker.

<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C51e44c9b03c66" class=" tableEdit "
|-
!|Browse By Plasmid Type:!!
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mammalian Mammalian Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Bacterial Bacterial Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Luciferase Luciferase]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mouse Mouse Targeting]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Retroviral Retroviral]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Lentiviral Lentiviral]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Worm Worm Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Insect Insect Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Yeast Yeast Expression, E.Coli or yeast]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:RNAi RNAi]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.C51e44c9b03c66&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

===Searching===
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P51e44b532cef6" class=" tableEdit "
|-
!|Additional Searches By:!!
|-
|
Bacterial Resistance
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Ampicillin Ampicillin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Kanamycin Kanamycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Tetracycline Tetracycline]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Chloramphenicol Chloramphenicol]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Spectinomycin Spectinomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Gentamycin Gentamycin]
|-
|
Selectable Marker
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Neomycin Neomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Puromycin Puromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Zeocin Zeocin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:URA3 URA3]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:LEU2 LEU2]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:TRP1 TRP1];[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:HIS3 HIS3]
|-
|
Source
|
[http://www.addgene.org/vector-database/query/?q_vdb=source:addgene Addgene]; [http://www.addgene.org/vector-database/query/?q_vdb=source:ATCC ATCC]; [http://www.addgene.org/vector-database/query/?q_vdb=source:BD%20%20Biosciences BD Biosciences]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Invitrogen Invitrogen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Novagen Novagen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Sigma Sigma]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Stratagene Stratagene]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.P51e44b532cef6&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

Addgene

2014-07-22T13:56:10Z

Addgene: /* Content{{HelpIcon|Help:Databases}} */

{{Pagetop}}
[[File:Addgene New Logo.jpg|center]]
<protect>

{| {{Prettytable}} width='30%' align="right" id="J48049604a1b31" class=" tableEdit Database_table"

|-
!align=left {{table heading style}} |Link/URL:
||
[http://www.addgene.org/vector-database/ www.Addgene.org]
|-
!align=left {{table heading style}} |What:
||
Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) [http://www.addgene.org/vector-database/query/?q_vdb=#searchbar Click to search vector database now].
|-
!align=left {{table heading style}} |Who:
||
Addgene is a nonprofit global plasmid repository dedicated to helping scientists share their plasmids.
|-
!align=left {{table heading style}} |Updates:
||
Do you use plasmids?

Join the non-profit plasmid sharing community. Visit [http://www.addgene.org/ www.addgene.org] to learn more, find plasmids, and start depositing!
|-
!align=left {{table heading style}} |Upcoming events:
||
Visit [http://www.addgene.org/news/ Addgene's News and Announcements page] to see current news and announcements.
|-
!align=left {{table heading style}} |Web Services:
||
[http://www.addgene.org/ Addgene] is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J48049604a1b31&page=133952&pagename={{FULLPAGENAMEE}}&type=1&template=Database_table edit table] ||
|}
</protect>
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.

==Content{{HelpIcon|Help:Databases}}==
'''Plasmid Repository'''

As of 2014, Addgene's repository stores and distributes over 30,000 plasmids, contributed by over 2,000 depositing labs. Addgene's collection contains plasmids used for a variety of functions such as genome engineering (including CRISPR systems), gene expression, shRNA knockdown, viral-mediated gene deliveray, detection of miRNA, and promoter activity.

The plasmid collection includes:

* Genome engineering
** [http://www.addgene.org/CRISPR/ CRISPR]s
** [http://www.addgene.org/TALEN/guide/ Transcription Activator-Like Effector Nuclease] (TALEN) kits
** [http://www.addgene.org/zfc/ Zinc finger nuclease] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [http://www.addgene.org/fluorescent_proteins/ Fluorescent marker]
* Viral vectors
** [http://www.addgene.org/lentiviral/ Retroviral/Lentiviral]
** Adenoviral
** Adeno-associated virus
* cDNA expression
* shRNA expression

==Tools and guides==

'''Molecular Biology Tools'''

[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

'''Plasmid Cloning Guides'''

[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==
===Browsing===
[http://www.addgene.org/vector-database/ Browse Plasmid Repository] by plasmid type, source, bacterial resistance, and selectable marker.

<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C51e44c9b03c66" class=" tableEdit "
|-
!|Browse By Plasmid Type:!!
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mammalian Mammalian Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Bacterial Bacterial Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Luciferase Luciferase]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mouse Mouse Targeting]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Retroviral Retroviral]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Lentiviral Lentiviral]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Worm Worm Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Insect Insect Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Yeast Yeast Expression, E.Coli or yeast]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:RNAi RNAi]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.C51e44c9b03c66&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

===Searching===
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P51e44b532cef6" class=" tableEdit "
|-
!|Additional Searches By:!!
|-
|
Bacterial Resistance
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Ampicillin Ampicillin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Kanamycin Kanamycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Tetracycline Tetracycline]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Chloramphenicol Chloramphenicol]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Spectinomycin Spectinomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Gentamycin Gentamycin]
|-
|
Selectable Marker
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Neomycin Neomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Puromycin Puromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Zeocin Zeocin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:URA3 URA3]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:LEU2 LEU2]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:TRP1 TRP1];[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:HIS3 HIS3]
|-
|
Source
|
[http://www.addgene.org/vector-database/query/?q_vdb=source:addgene Addgene]; [http://www.addgene.org/vector-database/query/?q_vdb=source:ATCC ATCC]; [http://www.addgene.org/vector-database/query/?q_vdb=source:BD%20%20Biosciences BD Biosciences]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Invitrogen Invitrogen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Novagen Novagen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Sigma Sigma]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Stratagene Stratagene]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.P51e44b532cef6&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

Addgene

2014-07-22T13:55:02Z

Addgene: Table edited by Addgene via TableEdit

{{Pagetop}}
[[File:Addgene New Logo.jpg|center]]
<protect>

{| {{Prettytable}} width='30%' align="right" id="J48049604a1b31" class=" tableEdit Database_table"

|-
!align=left {{table heading style}} |Link/URL:
||
[http://www.addgene.org/vector-database/ www.Addgene.org]
|-
!align=left {{table heading style}} |What:
||
Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) [http://www.addgene.org/vector-database/query/?q_vdb=#searchbar Click to search vector database now].
|-
!align=left {{table heading style}} |Who:
||
Addgene is a nonprofit global plasmid repository dedicated to helping scientists share their plasmids.
|-
!align=left {{table heading style}} |Updates:
||
Do you use plasmids?

Join the non-profit plasmid sharing community. Visit [http://www.addgene.org/ www.addgene.org] to learn more, find plasmids, and start depositing!
|-
!align=left {{table heading style}} |Upcoming events:
||
Visit [http://www.addgene.org/news/ Addgene's News and Announcements page] to see current news and announcements.
|-
!align=left {{table heading style}} |Web Services:
||
[http://www.addgene.org/ Addgene] is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J48049604a1b31&page=133952&pagename={{FULLPAGENAMEE}}&type=1&template=Database_table edit table] ||
|}
</protect>
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.

==Content{{HelpIcon|Help:Databases}}==
'''Plasmid Repository'''

In 2014 Addgene's repository distributes 30,000 types of plasmids, deposited by 1,700 labs.
Addgene’s plasmid collection contains plasmids used for a variety of functions such as [http://www.addgene.org/Genome_Engineering/ genome engineering] (including CRISPRS), gene expression, shRNA knockdown, viral-mediated gene delivery, detection of miRNA and promoter activity.

The plasmid collection includes:

* Genome engineering
** [http://www.addgene.org/CRISPR/ CRISPR]s
** [http://www.addgene.org/TALEN/guide/ Transcription Activator-Like Effector Nuclease] (TALEN) kits
** [http://www.addgene.org/zfc/ Zinc finger nuclease] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [http://www.addgene.org/fluorescent_proteins/ Fluorescent marker]
* Viral vectors
** [http://www.addgene.org/lentiviral/ Retroviral/Lentiviral]
** Adenoviral
** Adeno-associated virus
* cDNA expression
* shRNA expression

==Tools and guides==

'''Molecular Biology Tools'''

[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

'''Plasmid Cloning Guides'''

[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==
===Browsing===
[http://www.addgene.org/vector-database/ Browse Plasmid Repository] by plasmid type, source, bacterial resistance, and selectable marker.

<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C51e44c9b03c66" class=" tableEdit "
|-
!|Browse By Plasmid Type:!!
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mammalian Mammalian Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Bacterial Bacterial Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Luciferase Luciferase]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mouse Mouse Targeting]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Retroviral Retroviral]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Lentiviral Lentiviral]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Worm Worm Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Insect Insect Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Yeast Yeast Expression, E.Coli or yeast]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:RNAi RNAi]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.C51e44c9b03c66&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

===Searching===
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P51e44b532cef6" class=" tableEdit "
|-
!|Additional Searches By:!!
|-
|
Bacterial Resistance
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Ampicillin Ampicillin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Kanamycin Kanamycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Tetracycline Tetracycline]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Chloramphenicol Chloramphenicol]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Spectinomycin Spectinomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Gentamycin Gentamycin]
|-
|
Selectable Marker
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Neomycin Neomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Puromycin Puromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Zeocin Zeocin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:URA3 URA3]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:LEU2 LEU2]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:TRP1 TRP1];[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:HIS3 HIS3]
|-
|
Source
|
[http://www.addgene.org/vector-database/query/?q_vdb=source:addgene Addgene]; [http://www.addgene.org/vector-database/query/?q_vdb=source:ATCC ATCC]; [http://www.addgene.org/vector-database/query/?q_vdb=source:BD%20%20Biosciences BD Biosciences]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Invitrogen Invitrogen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Novagen Novagen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Sigma Sigma]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Stratagene Stratagene]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.P51e44b532cef6&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

Addgene

2014-07-22T13:53:55Z

Addgene: /* About {{PAGENAME}} {{HelpIcon|Help:Databases}} */

{{Pagetop}}
[[File:Addgene New Logo.jpg|center]]
<protect>

{| {{Prettytable}} width='30%' align="right" id="J48049604a1b31" class=" tableEdit Database_table"

|-
!align=left {{table heading style}} |Link/URL:
||
[http://www.addgene.org/vector-database/ www.Addgene.org]
||

|-
!align=left {{table heading style}} |What:
||
Addgene hosts a vector database (a free digital collection of vector backbones assembled by Addgene from publications and commercially available sources) [http://www.addgene.org/vector-database/query/?q_vdb=#searchbar Click to search vector database now].
||

|-
!align=left {{table heading style}} |Who:
||
Addgene is a non-profit global plasmid repository dedicated to helping scientists share their plasmids.
||

|-
!align=left {{table heading style}} |Updates:
||
Do you use plasmids?

Join the non-profit plasmid sharing community. Visit [http://www.addgene.org/ www.addgene.org] to learn more, find plasmids, and start depositing!
||

|-
!align=left {{table heading style}} |Upcoming events:
||
Visit [http://www.addgene.org/news/ Addgene's News and Announcements page] to see current news and announcements.
||

|-
!align=left {{table heading style}} |Web Services:
||
[http://www.addgene.org/ Addgene] is a non-profit plasmid repository dedicated to collecting, curating, and distributing plasmids for use in academic and non-profit research to scientists around the world.
||

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.J48049604a1b31&page=133952&pagename={{FULLPAGENAMEE}}&type=1&template=Database_table edit table] || ||
|}
</protect>
{{Database_table_help}}
==About {{PAGENAME}} {{HelpIcon|Help:Databases}}==
Addgene is a nonprofit organization dedicated to making it easier for scientists to share plasmids.

Addgene is reaching this goal by operating a plasmid repository for the research community. We are working with thousands of laboratories to assemble a high-quality library of published plasmids for use in research and discovery. By linking plasmids with articles, scientists can always find data related to the materials they request.

==Content{{HelpIcon|Help:Databases}}==
'''Plasmid Repository'''

In 2014 Addgene's repository distributes 30,000 types of plasmids, deposited by 1,700 labs.
Addgene’s plasmid collection contains plasmids used for a variety of functions such as [http://www.addgene.org/Genome_Engineering/ genome engineering] (including CRISPRS), gene expression, shRNA knockdown, viral-mediated gene delivery, detection of miRNA and promoter activity.

The plasmid collection includes:

* Genome engineering
** [http://www.addgene.org/CRISPR/ CRISPR]s
** [http://www.addgene.org/TALEN/guide/ Transcription Activator-Like Effector Nuclease] (TALEN) kits
** [http://www.addgene.org/zfc/ Zinc finger nuclease] kits
* Empty backbones
** Species-specific expression
** Epitope tags
** Fusion proteins
** Selectable markers
** [http://www.addgene.org/fluorescent_proteins/ Fluorescent marker]
* Viral vectors
** [http://www.addgene.org/lentiviral/ Retroviral/Lentiviral]
** Adenoviral
** Adeno-associated virus
* cDNA expression
* shRNA expression

==Tools and guides==

'''Molecular Biology Tools'''

[http://www.addgene.org/vector-database/ Vector Database]- A curated list of over 4,000 commonly used vector backbones, including relevant cloning information and bacterial growth conditions.

[http://www.addgene.org/analyze-sequence/ Sequence Analyzer]- A software tool developed by Addgene to create plasmid maps from sequences with annotated features and restriction sites.

[http://www.addgene.org/mol_bio_reference/ Molecular Biology Reference]- A collection of references for commonly used molecular biology reagents, such as primers, restriction enzymes, and antibiotic concentrations.

'''Plasmid Cloning Guides'''

[http://www.addgene.org/plasmid_reference/ Molecular Cloning Guides]- References to help scientists design their plasmid cloning experiments, including tutorials on restriction enzyme digestion and PCR-based cloning.

[http://www.addgene.org/plasmid_reference/index/ Molecular Cloning Protocols]- Specific protocols for a variety of plasmid cloning techniques, such as isolation of bacterial colonies, DNA purification by gel electrophoresis, and bacterial transformation.

==Using {{PAGENAME}} {{HelpIcon|Help:Databases}}==
===Browsing===
[http://www.addgene.org/vector-database/ Browse Plasmid Repository] by plasmid type, source, bacterial resistance, and selectable marker.

<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C51e44c9b03c66" class=" tableEdit "
|-
!|Browse By Plasmid Type:!!
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mammalian Mammalian Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Bacterial Bacterial Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Luciferase Luciferase]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Mouse Mouse Targeting]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Retroviral Retroviral]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Lentiviral Lentiviral]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Worm Worm Expression]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Insect Insect Expression]
|-
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:Yeast Yeast Expression, E.Coli or yeast]
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20vector_type:RNAi RNAi]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.C51e44c9b03c66&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>

===Searching===
<protect>

{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="P51e44b532cef6" class=" tableEdit "
|-
!|Additional Searches By:!!
|-
|
Bacterial Resistance
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Ampicillin Ampicillin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Kanamycin Kanamycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Tetracycline Tetracycline]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Chloramphenicol Chloramphenicol]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Spectinomycin Spectinomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20resistance_strain:Gentamycin Gentamycin]
|-
|
Selectable Marker
|
[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Neomycin Neomycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Puromycin Puromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Hygromycin Hygromycin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:Zeocin Zeocin]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:URA3 URA3]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:LEU2 LEU2]; [http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:TRP1 TRP1];[http://www.addgene.org/vector-database/query/?q_vdb=*+***%20selectable_markers:HIS3 HIS3]
|-
|
Source
|
[http://www.addgene.org/vector-database/query/?q_vdb=source:addgene Addgene]; [http://www.addgene.org/vector-database/query/?q_vdb=source:ATCC ATCC]; [http://www.addgene.org/vector-database/query/?q_vdb=source:BD%20%20Biosciences BD Biosciences]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Invitrogen Invitrogen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Novagen Novagen]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Sigma Sigma]; [http://www.addgene.org/vector-database/query/?q_vdb=source:Stratagene Stratagene]

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.133952.P51e44b532cef6&page=133952&pagename={{FULLPAGENAMEE}}&type=0&template= edit table] ||
|}
</protect>