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	<updated>2026-11-03T20:34:48Z</updated>
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	<entry>
		<id>https://ecoliwiki.org/colipedia/index.php?title=PMID:6266829&amp;diff=1198474</id>
		<title>PMID:6266829</title>
		<link rel="alternate" type="text/html" href="https://ecoliwiki.org/colipedia/index.php?title=PMID:6266829&amp;diff=1198474"/>
		<updated>2010-09-10T18:50:34Z</updated>

		<summary type="html">&lt;p&gt;91.214.45.227: Fill PMID: Page!&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{RightTOC}}&lt;br /&gt;
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&amp;lt;span style = &#039;font-size: large&#039;&amp;gt; &#039;&#039;&#039;Ovchinnikov, YA, Monastyrskaya, GS, Gubanov, VV, Guryev, SO, Chertov OYu, NN, Modyanov, VA, Grinkevich, IA, Makarova, TV, Marchenko, IN, Polovnikova, VM, Lipkin, ED and Sverdlov, &#039;&#039;&#039;  (1981) The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit.&#039;&#039;Eur. J. Biochem.&#039;&#039; &#039;&#039;&#039;116&#039;&#039;&#039;:621-9&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{|{{Prettytable}} align = &#039;center&#039; width= &#039;70%&#039;&lt;br /&gt;
|-&lt;br /&gt;
|{{TableHeadingColor}} |&#039;&#039;&#039;Abstract&#039;&#039;&#039; || &amp;lt;protect&amp;gt;The combined structural study of proteins and of their corresponding genes utilizing the methods of both protein and nucleotide chemistry greatly accelerates and considerably simplifies both the nucleotide and protein structure determination and, in particular, enhances the reliability of the analysis. This approach has been successfully applied in the primary structure determination of the beta and beta&#039; subunits of Escherichia coli DNA-dependent RNA polymerase and of their structural genes, yielding a continuous nucleotide sequence (4714 base pairs) that embraces the entire rpoB gene, the initial part of the rpoC gene and the intercistronic region, together with the total amino acid sequence of the beta subunit, comprising 1342 residues, and the N-terminal sequence of the beta&#039; subunit (176 residues).&amp;lt;/protect&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|{{TableHeadingColor}} nowrap |&#039;&#039;&#039;Links&#039;&#039;&#039; ||&lt;br /&gt;
*  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=pubmed&amp;amp;dopt=Abstract&amp;amp;list_uids=6266829 PubMed]&lt;br /&gt;
&lt;br /&gt;
|}&lt;br /&gt;
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==Significance {{HelpIcon|Help:Literature}} ==&lt;br /&gt;
{{LitSignificance}}&lt;br /&gt;
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{|   id=&amp;quot;E4c8a7dfa9297f&amp;quot;  class=&amp;quot; tableEdit GO_table_reference&amp;quot;  &lt;br /&gt;
|- {{table heading style}}&lt;br /&gt;
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status&lt;br /&gt;
&lt;br /&gt;
|- class=&amp;quot;tableEdit_footer&amp;quot; &lt;br /&gt;
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{{RefHelp}}&lt;br /&gt;
&amp;lt;references/&amp;gt;&lt;/div&gt;</summary>
		<author><name>91.214.45.227</name></author>
	</entry>
	<entry>
		<id>https://ecoliwiki.org/colipedia/index.php?title=PMID:6266829&amp;diff=1198473</id>
		<title>PMID:6266829</title>
		<link rel="alternate" type="text/html" href="https://ecoliwiki.org/colipedia/index.php?title=PMID:6266829&amp;diff=1198473"/>
		<updated>2010-09-10T18:50:34Z</updated>

		<summary type="html">&lt;p&gt;91.214.45.227: New PMID: Page!&lt;/p&gt;
&lt;hr /&gt;
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		<author><name>91.214.45.227</name></author>
	</entry>
	<entry>
		<id>https://ecoliwiki.org/colipedia/index.php?title=Category:GO:0004488_!_methylenetetrahydrofolate_dehydrogenase_(NADP%2B)_activity&amp;diff=1198472</id>
		<title>Category:GO:0004488 ! methylenetetrahydrofolate dehydrogenase (NADP+) activity</title>
		<link rel="alternate" type="text/html" href="https://ecoliwiki.org/colipedia/index.php?title=Category:GO:0004488_!_methylenetetrahydrofolate_dehydrogenase_(NADP%2B)_activity&amp;diff=1198472"/>
		<updated>2010-09-10T16:32:21Z</updated>

		<summary type="html">&lt;p&gt;91.214.45.227: New GO term category page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{GO term stub|GO:0004488_!_methylenetetrahydrofolate_dehydrogenase_(NADP+)_activity}}&lt;/div&gt;</summary>
		<author><name>91.214.45.227</name></author>
	</entry>
	<entry>
		<id>https://ecoliwiki.org/colipedia/index.php?title=Category:GO:0004477_!_methenyltetrahydrofolate_cyclohydrolase_activity&amp;diff=1198471</id>
		<title>Category:GO:0004477 ! methenyltetrahydrofolate cyclohydrolase activity</title>
		<link rel="alternate" type="text/html" href="https://ecoliwiki.org/colipedia/index.php?title=Category:GO:0004477_!_methenyltetrahydrofolate_cyclohydrolase_activity&amp;diff=1198471"/>
		<updated>2010-09-10T16:32:17Z</updated>

		<summary type="html">&lt;p&gt;91.214.45.227: New GO term category page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{GO term stub|GO:0004477_!_methenyltetrahydrofolate_cyclohydrolase_activity}}&lt;/div&gt;</summary>
		<author><name>91.214.45.227</name></author>
	</entry>
	<entry>
		<id>https://ecoliwiki.org/colipedia/index.php?title=Category:GO:0004834_!_tryptophan_synthase_activity&amp;diff=1198468</id>
		<title>Category:GO:0004834 ! tryptophan synthase activity</title>
		<link rel="alternate" type="text/html" href="https://ecoliwiki.org/colipedia/index.php?title=Category:GO:0004834_!_tryptophan_synthase_activity&amp;diff=1198468"/>
		<updated>2010-09-10T12:12:05Z</updated>

		<summary type="html">&lt;p&gt;91.214.45.227: New GO term category page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{GO term stub|GO:0004834_!_tryptophan_synthase_activity}}&lt;/div&gt;</summary>
		<author><name>91.214.45.227</name></author>
	</entry>
	<entry>
		<id>https://ecoliwiki.org/colipedia/index.php?title=Category:GO:0043199_!_sulfate_binding&amp;diff=1186095</id>
		<title>Category:GO:0043199 ! sulfate binding</title>
		<link rel="alternate" type="text/html" href="https://ecoliwiki.org/colipedia/index.php?title=Category:GO:0043199_!_sulfate_binding&amp;diff=1186095"/>
		<updated>2010-08-01T22:41:29Z</updated>

		<summary type="html">&lt;p&gt;91.214.45.227: New GO term category page&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{GO term stub|GO:0043199_!_sulfate_binding}}&lt;/div&gt;</summary>
		<author><name>91.214.45.227</name></author>
	</entry>
	<entry>
		<id>https://ecoliwiki.org/colipedia/index.php?title=PMID:3290203&amp;diff=1163218</id>
		<title>PMID:3290203</title>
		<link rel="alternate" type="text/html" href="https://ecoliwiki.org/colipedia/index.php?title=PMID:3290203&amp;diff=1163218"/>
		<updated>2010-06-25T23:17:46Z</updated>

		<summary type="html">&lt;p&gt;91.214.45.227: Fill PMID: Page!&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;{{RightTOC}}&lt;br /&gt;
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&amp;lt;span style = &#039;font-size: large&#039;&amp;gt; &#039;&#039;&#039;Ahrweiler, PM and Frieden, C&#039;&#039;&#039;  (1988) Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments.&#039;&#039;J. Bacteriol.&#039;&#039; &#039;&#039;&#039;170&#039;&#039;&#039;:3301-4&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{|{{Prettytable}} align = &#039;center&#039; width= &#039;70%&#039;&lt;br /&gt;
|-&lt;br /&gt;
|{{TableHeadingColor}} |&#039;&#039;&#039;Abstract&#039;&#039;&#039; || &amp;lt;protect&amp;gt;A strain of Escherichia coli with the fol gene deleted and a kan gene inserted in its place was created for use in cloning and isolation of mutant dihydrofolate reductase. Southern blot analysis and dihydrofolate reductase enzyme assays confirmed the delta fol::kan genotype. A thyA mutation accompanied the fol deletion and is required for survival of a dihydrofolate reductase-deficient strain.&amp;lt;/protect&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|{{TableHeadingColor}} nowrap |&#039;&#039;&#039;Links&#039;&#039;&#039; ||&lt;br /&gt;
*  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=pubmed&amp;amp;dopt=Abstract&amp;amp;list_uids=3290203 PubMed]&lt;br /&gt;
&lt;br /&gt;
|}&lt;br /&gt;
&amp;lt;/protect&amp;gt;&lt;br /&gt;
==Significance {{HelpIcon|Help:Literature}} ==&lt;br /&gt;
{{LitSignificance}}&lt;br /&gt;
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{|   id=&amp;quot;R4c25391a6dda2&amp;quot;  class=&amp;quot; tableEdit GO_table_reference&amp;quot;  &lt;br /&gt;
|- {{table heading style}}&lt;br /&gt;
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status&lt;br /&gt;
&lt;br /&gt;
|- class=&amp;quot;tableEdit_footer&amp;quot; &lt;br /&gt;
|&amp;lt;span class=&amp;quot;tableEdit_editLink plainlinks&amp;quot;&amp;gt;[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&amp;amp;id=63ce1649aab8b3180235b0af7618f667.172174.R4c25391a6dda2&amp;amp;page=172174&amp;amp;pagename={{FULLPAGENAMEE}}&amp;amp;type=0&amp;amp;template=GO_table_reference edit table]&amp;lt;/span&amp;gt; || || || || || || || ||&lt;br /&gt;
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		<author><name>91.214.45.227</name></author>
	</entry>
	<entry>
		<id>https://ecoliwiki.org/colipedia/index.php?title=PMID:3290203&amp;diff=1163217</id>
		<title>PMID:3290203</title>
		<link rel="alternate" type="text/html" href="https://ecoliwiki.org/colipedia/index.php?title=PMID:3290203&amp;diff=1163217"/>
		<updated>2010-06-25T23:17:46Z</updated>

		<summary type="html">&lt;p&gt;91.214.45.227: New PMID: Page!&lt;/p&gt;
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		<author><name>91.214.45.227</name></author>
	</entry>
	<entry>
		<id>https://ecoliwiki.org/colipedia/index.php?title=PMID:4610415&amp;diff=1163216</id>
		<title>PMID:4610415</title>
		<link rel="alternate" type="text/html" href="https://ecoliwiki.org/colipedia/index.php?title=PMID:4610415&amp;diff=1163216"/>
		<updated>2010-06-25T23:17:38Z</updated>

		<summary type="html">&lt;p&gt;91.214.45.227: Fill PMID: Page!&lt;/p&gt;
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&amp;lt;span style = &#039;font-size: large&#039;&amp;gt; &#039;&#039;&#039;Bennett, CD&#039;&#039;&#039;  (1974) Letter: Primary structure of E. coli dihydrofolate reductase.&#039;&#039;Nature&#039;&#039; &#039;&#039;&#039;252&#039;&#039;&#039;:511-2&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{|{{Prettytable}} align = &#039;center&#039; width= &#039;70%&#039;&lt;br /&gt;
|-&lt;br /&gt;
|{{TableHeadingColor}} |&#039;&#039;&#039;Abstract&#039;&#039;&#039; || &amp;lt;protect&amp;gt;No abstract in PubMed&amp;lt;/protect&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|{{TableHeadingColor}} nowrap |&#039;&#039;&#039;Links&#039;&#039;&#039; ||&lt;br /&gt;
*  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=pubmed&amp;amp;dopt=Abstract&amp;amp;list_uids=4610415 PubMed]&lt;br /&gt;
&lt;br /&gt;
|}&lt;br /&gt;
&amp;lt;/protect&amp;gt;&lt;br /&gt;
==Significance {{HelpIcon|Help:Literature}} ==&lt;br /&gt;
{{LitSignificance}}&lt;br /&gt;
&lt;br /&gt;
== Useful Materials and Methods {{HelpIcon|Help:Literature}}==&lt;br /&gt;
{{LitMaterials}}&lt;br /&gt;
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|- {{table heading style}}&lt;br /&gt;
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status&lt;br /&gt;
&lt;br /&gt;
|- class=&amp;quot;tableEdit_footer&amp;quot; &lt;br /&gt;
|&amp;lt;span class=&amp;quot;tableEdit_editLink plainlinks&amp;quot;&amp;gt;[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&amp;amp;id=63ce1649aab8b3180235b0af7618f667.172173.V4c253912c359b&amp;amp;page=172173&amp;amp;pagename={{FULLPAGENAMEE}}&amp;amp;type=0&amp;amp;template=GO_table_reference edit table]&amp;lt;/span&amp;gt; || || || || || || || ||&lt;br /&gt;
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&amp;lt;references/&amp;gt;&lt;/div&gt;</summary>
		<author><name>91.214.45.227</name></author>
	</entry>
	<entry>
		<id>https://ecoliwiki.org/colipedia/index.php?title=PMID:4610415&amp;diff=1163215</id>
		<title>PMID:4610415</title>
		<link rel="alternate" type="text/html" href="https://ecoliwiki.org/colipedia/index.php?title=PMID:4610415&amp;diff=1163215"/>
		<updated>2010-06-25T23:17:38Z</updated>

		<summary type="html">&lt;p&gt;91.214.45.227: New PMID: Page!&lt;/p&gt;
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		<author><name>91.214.45.227</name></author>
	</entry>
	<entry>
		<id>https://ecoliwiki.org/colipedia/index.php?title=PMID:350268&amp;diff=1163214</id>
		<title>PMID:350268</title>
		<link rel="alternate" type="text/html" href="https://ecoliwiki.org/colipedia/index.php?title=PMID:350268&amp;diff=1163214"/>
		<updated>2010-06-25T23:16:59Z</updated>

		<summary type="html">&lt;p&gt;91.214.45.227: Fill PMID: Page!&lt;/p&gt;
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&amp;lt;span style = &#039;font-size: large&#039;&amp;gt; &#039;&#039;&#039;Bennett, CD, Rodkey, JA, Sondey, JM and Hirschmann, R&#039;&#039;&#039;  (1978) Dihydrofolate reductase: the amino acid sequence of the enzyme from a methotrexate-resistant mutant of Escherichia coli.&#039;&#039;Biochemistry&#039;&#039; &#039;&#039;&#039;17&#039;&#039;&#039;:1328-37&amp;lt;/span&amp;gt;&lt;br /&gt;
&lt;br /&gt;
{|{{Prettytable}} align = &#039;center&#039; width= &#039;70%&#039;&lt;br /&gt;
|-&lt;br /&gt;
|{{TableHeadingColor}} |&#039;&#039;&#039;Abstract&#039;&#039;&#039; || &amp;lt;protect&amp;gt;The determination of the amino acid sequence of the enzyme dihydrofolate reductase (5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3) from a mutant of Escherichia coli B is described. The 159 residues were positioned by automatic Edman degradation of the whole protein, of the reduced and alkylated cyanogen bromide fragments, and of selected tryptic, chymotryptic, and thermolytic digestion products. An N-bromosuccinimide produced fragment of the largest cyanogen bromide peptide was also used in the sequence determination.&amp;lt;/protect&amp;gt;&lt;br /&gt;
|-&lt;br /&gt;
|{{TableHeadingColor}} nowrap |&#039;&#039;&#039;Links&#039;&#039;&#039; ||&lt;br /&gt;
*  [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;amp;db=pubmed&amp;amp;dopt=Abstract&amp;amp;list_uids=350268 PubMed]&lt;br /&gt;
&lt;br /&gt;
|}&lt;br /&gt;
&amp;lt;/protect&amp;gt;&lt;br /&gt;
==Significance {{HelpIcon|Help:Literature}} ==&lt;br /&gt;
{{LitSignificance}}&lt;br /&gt;
&lt;br /&gt;
== Useful Materials and Methods {{HelpIcon|Help:Literature}}==&lt;br /&gt;
{{LitMaterials}}&lt;br /&gt;
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{|   id=&amp;quot;B4c2538eb96397&amp;quot;  class=&amp;quot; tableEdit GO_table_reference&amp;quot;  &lt;br /&gt;
|- {{table heading style}}&lt;br /&gt;
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status&lt;br /&gt;
&lt;br /&gt;
|- class=&amp;quot;tableEdit_footer&amp;quot; &lt;br /&gt;
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		<author><name>91.214.45.227</name></author>
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	<entry>
		<id>https://ecoliwiki.org/colipedia/index.php?title=PMID:350268&amp;diff=1163213</id>
		<title>PMID:350268</title>
		<link rel="alternate" type="text/html" href="https://ecoliwiki.org/colipedia/index.php?title=PMID:350268&amp;diff=1163213"/>
		<updated>2010-06-25T23:16:59Z</updated>

		<summary type="html">&lt;p&gt;91.214.45.227: New PMID: Page!&lt;/p&gt;
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