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PMID:22295907

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'''Stratmann, T, Pul, Ü, Wurm, R, Wagner, R and Schnetz, K''' (2012) RcsB-BglJ activates the Escherichia coli leuO gene, encoding an H-NS antagonist and pleiotropic regulator of virulence determinants. ''Mol. Microbiol.'' '''83''':1109-23
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!align=left |Abstract
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The LysR-type transcription factor LeuO is involved in regulation of pathogenicity determinants and stress responses in Enterobacteriaceae, and acts as antagonist of the global repressor H-NS. Expression of the leuO gene is repressed by H-NS, and it is upregulated in stationary phase and under amino acid starvation conditions. Here, we show that the heterodimer of the FixJ/NarL-type transcription regulators RcsB and BglJ strongly activates expression of leuO and that RcsB-BglJ regulates additional loci. Activation of leuO by RcsB-BglJ is independent of the Rcs phosphorelay system. RcsB-BglJ binds to the leuO promoter region and activates one of two leuO promoters mapped in vivo. Moreover, LeuO antagonizes activation of leuO by RcsB-BglJ and acts as negative autoregulator in vivo and in vitro. Further, the H-NS paralogue StpA causes repression of leuO in addition to H-NS. Together, our data suggest a complex arrangement of regulatory elements and they indicate a feedback control mechanism of leuO expression.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=22295907 PubMed]
Online version:[http://dx.doi.org/10.1111/j.1365-2958.2012.07993.x 10.1111/j.1365-2958.2012.07993.x]
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!align=left |Keywords
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Base Sequence; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/antagonists & inhibitors; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Fimbriae Proteins/antagonists & inhibitors; Fimbriae Proteins/genetics; Fimbriae Proteins/metabolism; Gene Expression Regulation, Bacterial; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Molecular Sequence Data; Operon; Promoter Regions, Genetic; Protein Binding; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcriptional Activation; Virulence Factors/genetics; Virulence Factors/metabolism

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==Significance {{HelpIcon|Help:Literature}} ==
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== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
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==Annotations {{HelpIcon|Help:Literature}}==
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
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==See also==

==References==
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PMID:22295907

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PMID:20444105

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'''Kendall, MM, Rasko, DA and Sperandio, V''' (2010) The LysR-type regulator QseA regulates both characterized and putative virulence genes in enterohaemorrhagic Escherichia coli O157:H7. ''Mol. Microbiol.'' '''76''':1306-21
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!align=left |Abstract
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Enterohaemorrhagic Escherichia coli (EHEC) colonizes the large intestine, causing attaching and effacing (AE) lesions. Most of the genes involved in AE lesion formation are encoded within a chromosomal pathogenicity island termed the locus of enterocyte effacement (LEE). The LysR-type transcriptional factor QseA regulates the LEE by binding to the regulatory region of ler. We performed transcriptome analyses comparing wild-type (WT) EHEC and the qseA mutant to elucidate QseA's role in gene regulation. During both growth phases, several genes carried in O-islands were activated by QseA, whereas genes involved in cell metabolism were repressed. During late-logarithmic growth, QseA activated expression of the LEE genes as well as non-LEE-encoded effector proteins. We also performed electrophoretic mobility shift assays, competition experiments and DNase I footprints. The results demonstrated that QseA directly binds both the ler proximal and distal promoters, its own promoter, as well as promoters of genes encoded in EHEC-specific O-islands. Additionally, we mapped the transcriptional start site of qseA, leading to the identification of two promoter sequences. Taken together, these results indicate that QseA acts as a global regulator in EHEC, co-ordinating expression of virulence genes.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=20444105 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2936457 PMC2936457]
Online version:[http://dx.doi.org/10.1111/j.1365-2958.2010.07174.x 10.1111/j.1365-2958.2010.07174.x]
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!align=left |Keywords
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Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Escherichia coli O157/genetics; Escherichia coli O157/metabolism; Escherichia coli O157/pathogenicity; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Humans; Microarray Analysis; Molecular Sequence Data; Promoter Regions, Genetic; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<annotationlinks/>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
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==See also==

==References==
{{RefHelp}}
<references/>

PMID:20444105

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82.145.100.57: New PMID: Page!

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