https://ecoliwiki.org/colipedia/api.php?action=feedcontributions&feedformat=atom&user=74.6.19.80EcoliWiki - User contributions [en]2026-11-13T09:21:04ZUser contributionsMediaWiki 1.43.11https://ecoliwiki.org/colipedia/index.php?title=PMID:6089341&diff=317053PMID:60893412007-10-10T10:26:12Z<p>74.6.19.80: New PMID: Page!</p>
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<span style = 'font-size: large'> '''Van Dyke, MM and Dervan, PB''' (1984) Echinomycin binding sites on DNA.''Science'' '''225''':1122-7</span><br />
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|{{TableHeadingColor}} |'''Abstract''' || The preferred binding sites of echinomycin on DNA can be determined by a method called "footprinting." A 32P end-labeled restriction fragment from pBR322 DNA is protected by binding to echinomycin, and cleaved by a synthetic DNA cleaving reagent, methidiumpropyl--EDTA . Fe(II); the DNA cleavage products are then subjected to high-resolution gel analyses. This method reveals that echinomycin has a binding site size of four base pairs. The strong binding sites for echinomycin contain the central two-base-pair sequence 5'-CG-3'. From an analysis of 15 echinomycin sites on 210 base pairs of DNA, key recognition elements for echinomycin are contained in the sequences (5'-3') ACGT and TCGT (A, adenine; C, cytosine; G, guanine; T, thymine).<br />
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6089341 PubMed]<br />
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