EcoliWiki - User contributions [en]

PMID:4286570

2007-09-02T00:12:08Z

65.55.209.28: New PMID: Page!

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 '''Weigert, MG, Lanka, E and Garen, A''' (1965) Amino acid substitutions resulting from suppression of nonsense mutations. II. Glutamine insertion by the Su-2 gene; tyrosine insertion by the Su-3 gene.''J. Mol. Biol.'' '''14''':522-7

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|{{TableHeadingColor}} |'''Abstract''' || No abstract in PubMed
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4286570 PubMed]
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{|class = 'sortable' {{Prettytable}} id='220583'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.T46d9ffd7af764&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:2141650

2007-09-02T00:12:05Z

65.55.209.28: New PMID: Page!

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 '''Normanly, J, Kleina, LG, Masson, JM, Abelson, J and Miller, JH''' (1990) Construction of Escherichia coli amber suppressor tRNA genes. III. Determination of tRNA specificity.''J. Mol. Biol.'' '''213''':719-26

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|{{TableHeadingColor}} |'''Abstract''' || Using synthetic oligonucleotides, we have constructed a collection of Escherichia coli amber suppressor tRNA genes. In order to determine their specificities, these tRNAs were each used to suppress an amber (UAG) nonsense mutation in the E. coli dihydrofolate reductase gene fol. The mutant proteins were purified and subjected to N-terminal sequence analysis to determine which amino acid had been inserted by the suppressor tRNAs at the position of the amber codon. The suppressors can be classified into three groups on the basis of the protein sequence information. Class I suppressors, tRNA(CUAAla2), tRNA(CUAGly1), tRNA(CUAHisA), tRNA(CUALys) and tRNA(CUAProH), inserted the predicted amino acid. The class II suppressors, tRNA(CUAGluA), tRNA(CUAGly2) and tRNA(CUAIle1) were either partially or predominantly mischarged by the glutamine aminoacyl tRNA synthetase. The class III suppressors, tRNA(CUAArg), tRNA(CUAAspM), tRNA(CUAIle2), tRNA(CUAThr2), tRNA(CUAMet(m)) and tRNA(CUAVal) inserted predominantly lysine.
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2141650 PubMed]
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.E46d9ffd557e3d&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:16452957

2007-08-03T20:52:21Z

65.55.209.28: New PMID: Page!

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 '''Wang, K''' (2006) Gene-function wiki would let biologists pool worldwide resources.''Nature'' '''439''':534

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|{{TableHeadingColor}} |'''Abstract''' || No abstract in PubMed
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16452957 PubMed]
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{|class = 'sortable' {{Prettytable}} id='219997'
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!Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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|[{{SERVER}}/colipedia/index.php?title=Special:TableEdit&id=63ce1649aab8b3180235b0af7618f667.0.K46b39584a627a&page=0&pagename={{FULLPAGENAMEE}} edit table] || || || || || || || ||
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PMID:16397293

2007-07-26T10:21:37Z

65.55.209.28: New PMID: Page!

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 '''Riley, M, Abe, T, Arnaud, MB, Berlyn, MK, Blattner, FR, Chaudhuri, RR, Glasner, JD, Horiuchi, T, Keseler, IM, Kosuge, T, Mori, H, Perna, NT, Plunkett, G, Rudd, KE, Serres, MH, Thomas, GH, Thomson, NR, Wishart, D and Wanner, BL''' (2006) Escherichia coli K-12: a cooperatively developed annotation snapshot--2005.''Nucleic Acids Res.'' '''34''':1-9

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|{{TableHeadingColor}} |'''Abstract''' || The goal of this group project has been to coordinate and bring up-to-date information on all genes of Escherichia coli K-12. Annotation of the genome of an organism entails identification of genes, the boundaries of genes in terms of precise start and end sites, and description of the gene products. Known and predicted functions were assigned to each gene product on the basis of experimental evidence or sequence analysis. Since both kinds of evidence are constantly expanding, no annotation is complete at any moment in time. This is a snapshot analysis based on the most recent genome sequences of two E.coli K-12 bacteria. An accurate and up-to-date description of E.coli K-12 genes is of particular importance to the scientific community because experimentally determined properties of its gene products provide fundamental information for annotation of innumerable genes of other organisms. Availability of the complete genome sequence of two K-12 strains allows comparison of their genotypes and mutant status of alleles.
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16397293 PubMed]
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[{{SERVER}}/colipedia/index.php/Special:TableEdit?id=63ce1649aab8b3180235b0af7618f667.0.Y46a875b1d9f38&page=0&pagename={{FULLPAGENAMEE}}&type=0 Add GO annotations]
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PMID:3025174

2007-07-26T06:58:08Z

65.55.209.28: New PMID: Page!

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 '''Paek, KH and Walker, GC''' (1987) Escherichia coli dnaK null mutants are inviable at high temperature.''J. Bacteriol.'' '''169''':283-90

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|{{TableHeadingColor}} |'''Abstract''' || DnaK, a major Escherichia coli heat shock protein, is homologous to major heat shock proteins (Hsp70s) of Drosophila melanogaster and humans. Null mutations of the dnaK gene, both insertions and a deletion, were constructed in vitro and substituted for dnaK+ in the E. coli genome by homologous recombination in a recB recC sbcB strain. Cells carrying these dnaK null mutations grew slowly at low temperatures (30 and 37 degrees C) and could not form colonies at a high temperature (42 degrees C); furthermore, they also formed long filaments at 42 degrees C. The shift of the mutants to a high temperature evidently resulted in a loss of cell viability rather than simply an inhibition of growth since cells that had been incubated at 42 degrees C for 2 h were no longer capable of forming colonies at 30 degrees C. The introduction of a plasmid carrying the dnaK+ gene into these mutants restored normal cell growth and cell division at 42 degrees C. These null mutants showed a high basal level of synthesis of heat shock proteins except for DnaK, which was completely absent. In addition, the synthesis of heat shock proteins after induction in these dnaK null mutants was prolonged compared with that in a dnaK+ strain. The well-characterized dnaK756 mutation causes similar phenotypes, suggesting that they are caused by a loss rather than an alteration of DnaK function. The filamentation observed when dnaK mutations were incubated at a high temperature was not suppressed by sulA or sulB mutations, which suppress SOS-induced filamentation.(ABSTRACT TRUNCATED AT 250 WORDS)
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3025174 PubMed]
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