EcoliWiki - User contributions [en]

PMID:14888646

2012-06-11T19:59:58Z

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'''BERTANI, G''' (1951) Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli.''J. Bacteriol.'' '''62''':293-300
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!align=left |Abstract
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No abstract in PubMed
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14888646 PubMed]

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!align=left |Keywords
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Escherichia coli

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="A4fd64e3e6caaa" class=" tableEdit GO_table_reference dataTable"
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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==References==
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PMID:14888646

2012-06-11T19:59:45Z

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PMID:10840063

2012-06-10T17:54:34Z

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'''Mukhopadhyay, S and Chattoraj, DK''' (2000) Replication-induced transcription of an autorepressed gene: the replication initiator gene of plasmid P1.''Proc. Natl. Acad. Sci. U.S.A.'' '''97''':7142-7
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!align=left |Abstract
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The replication origin of plasmid P1 contains an array of five repeats (iterons) that bind the plasmid-encoded initiator RepA. Within the array lies the repA promoter, which becomes largely repressed on RepA binding (autorepression). One might expect that extra iterons produced on plasmid replication would titrate RepA and release the repression. The promoter, however, is induced poorly by extra iterons. The P1 copy number is reduced by extra iterons in the presence of the autorepressed repA gene but not when additional RepA is provided from constitutive sources. It has been proposed that the iteron-bound RepA couples with the promoter-bound RepA and thereby maintains repression. Although not the product of replication, we find that the act of replication itself can renew RepA synthesis. Replication apparently cleans the promoter of bound RepA and provides a window of opportunity for repA transcription. We propose that replication-induced transcription is required to ensure initiator availability in a system that is induced poorly when challenged with additional initiator binding sites.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10840063 PubMed]
Online version:[http://dx.doi.org/10.1073/pnas.130189497 10.1073/pnas.130189497]
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!align=left |Keywords
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DNA Helicases; DNA Replication; DNA-Binding Proteins; Gene Expression Regulation, Bacterial; Plasmids; Proteins; Replication Origin; Repressor Proteins; Trans-Activators; Transcription, Genetic

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="M4fd4df5a3fd37" class=" tableEdit GO_table_reference dataTable"
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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PMID:10840063

2012-06-10T17:54:31Z

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PMID:3074007

2012-06-10T16:10:04Z

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'''Abeles, AL and Austin, SJ''' (1988) P1 plasmid replication requires Escherichia coli Dam-methylated DNA.''Gene'' '''74''':185-6
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!align=left |Abstract
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No abstract in PubMed
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3074007 PubMed]

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!align=left |Keywords
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Bacterial Proteins; Base Sequence; DNA Replication; DNA, Bacterial; Escherichia coli; Escherichia coli Proteins; Methyltransferases; Molecular Sequence Data; Plasmids; Site-Specific DNA-Methyltransferase (Adenine-Specific)

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="T4fd4c6dc35a80" class=" tableEdit GO_table_reference dataTable"
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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PMID:3074007

2012-06-10T16:10:02Z

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PMID:1438220

2012-06-10T10:49:44Z

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{| id="W4fd47bc750580" class=" tableEdit PMID_info_table"

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'''Wickner, S, Skowyra, D, Hoskins, J and McKenney, K''' (1992) DnaJ, DnaK, and GrpE heat shock proteins are required in oriP1 DNA replication solely at the RepA monomerization step.''Proc. Natl. Acad. Sci. U.S.A.'' '''89''':10345-9
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!align=left |Abstract
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We have found that three Escherichia coli heat shock proteins, DnaK (the hsp70 homolog), DnaJ, and GrpE, function in oriP1 DNA replication in vitro solely to activate DNA binding by the replication initiator protein RepA. Activation results from the conversion of P1 or P7 RepA dimers to monomers that bind with high affinity to the origin of replication of plasmid P1. Thus, the essential role of these three heat shock proteins in this replication system is to change the quaternary structure of a single protein, RepA.
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1438220 PubMed]

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!align=left |Keywords
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Bacterial Proteins; DNA Helicases; DNA Replication; DNA, Bacterial; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Genetic Complementation Test; HSP40 Heat-Shock Proteins; HSP70 Heat-Shock Proteins; Heat-Shock Proteins; Kinetics; Models, Genetic; Models, Structural; Proteins; Trans-Activators

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="S4fd47bc824887" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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</protect>

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==References==
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<references/>

PMID:1438220

2012-06-10T10:49:39Z

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PMID:6384194

2012-06-10T03:07:40Z

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{| id="C4fd40f7c67a40" class=" tableEdit PMID_info_table"

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'''Sandulache, R, Prehm, P and Kamp, D''' (1984) Cell wall receptor for bacteriophage Mu G(+).''J. Bacteriol.'' '''160''':299-303
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!align=left |Abstract
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The invertible G segment in phage Mu DNA controls the host range of the phage. Depending on the orientation of the G segment, two types of phage particles, G(+) and G(-), are produced which recognize different cell surface receptors. The receptor for Mu G(+) was located in the lipopolysaccharide (LPS) of gram-negative bacteria. The analysis of different LPS core types and of mutants that were made resistant to Mu G(+) shows that the primary receptor site on Escherichia coli K-12 lies in the GlcNAc beta 1 . . . 6Glc alpha 1-2Glc alpha 1-part at the outer end of the LPS. Mu shares this receptor site in E. coli K-12 with the unrelated single-stranded DNA phage St-1. Phage D108, which is related to Mu, and phages P1 and P7, which are unrelated to Mu but contain a homologous invertible DNA segment, have different receptor requirements. Since they also bind to terminal glucose in a different configuration, they adsorb to and infect E. coli K-12 strains with an incomplete LPS core.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6384194 PubMed]

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!align=left |Keywords
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Coliphages; Escherichia coli; Lipopolysaccharides; Mutation; Receptors, Virus; Species Specificity

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

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{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="Y4fd40f7cc58bc" class=" tableEdit GO_table_reference dataTable"
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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PMID:6384194

2012-06-10T03:07:40Z

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PMID:7968916

2012-06-06T01:13:18Z

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{| id="R4fceaead8675b" class=" tableEdit PMID_info_table"

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'''Dreiseikelmann, B''' (1994) Translocation of DNA across bacterial membranes.''Microbiol. Rev.'' '''58''':293-316
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!align=left |Abstract
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DNA translocation across bacterial membranes occurs during the biological processes of infection by bacteriophages, conjugative DNA transfer of plasmids, T-DNA transfer, and genetic transformation. The mechanism of DNA translocation in these systems is not fully understood, but during the last few years extensive data about genes and gene products involved in the translocation processes have accumulated. One reason for the increasing interest in this topic is the discussion about horizontal gene transfer and transkingdom sex. Analyses of genes and gene products involved in DNA transfer suggest that DNA is transferred through a protein channel spanning the bacterial envelope. No common model exists for DNA translocation during phage infection. Perhaps various mechanisms are necessary as a result of the different morphologies of bacteriophages. The DNA translocation processes during conjugation, T-DNA transfer, and transformation are more consistent and may even be compared to the excretion of some proteins. On the basis of analogies and homologies between the proteins involved in DNA translocation and protein secretion, a common basic model for these processes is presented.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7968916 PubMed]

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!align=left |Keywords
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Bacteria; Cell Membrane; Conjugation, Genetic; DNA, Bacterial; DNA, Viral; Models, Genetic; Transduction, Genetic; Transformation, Bacterial

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="D4fceaeae09e09" class=" tableEdit GO_table_reference dataTable"
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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</protect>

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PMID:7968916

2012-06-06T01:13:17Z

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PMID:10931340

2012-06-05T19:19:12Z

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{| id="T4fce5bafe7708" class=" tableEdit PMID_info_table"

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'''Chattoraj, DK''' (2000) Control of plasmid DNA replication by iterons: no longer paradoxical.''Mol. Microbiol.'' '''37''':467-76
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!align=left |Abstract
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Replication origins of a family of bacterial plasmids have multiple sites, called iterons, for binding a plasmid-specific replication initiator protein. The iteron-initiator interactions are essential for plasmid replication as well as for inhibition of plasmid over-replication. The inhibition increases with plasmid copy number and eventually shuts plasmid replication off completely. The mechanism of inhibition appears to be handcuffing, the coupling of origins via iteron-bound initiators that block origin function. The probability of a trans-reaction such as handcuffing is expected to increase with plasmid copy number and diminish with increases in cell volume, explaining how the copy number can be maintained in a growing cell. Control is also exerted at the level of initiator synthesis and activation by chaperones. We propose that increases in active initiators promote initiation by overcoming handcuffing, but handcuffing dominates when the copy number reaches a threshold. Handcuffing should be ultrasensitive to copy number, as the negative control by iterons can be stringent (switch-like).
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10931340 PubMed]

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!align=left |Keywords
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DNA Replication; DNA, Bacterial; Plasmids

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

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{{LitMaterials}}

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<protect>

{| id="G4fce5bb0039ed" class=" tableEdit GO_table_reference dataTable"
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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PMID:10931340

2012-06-05T19:19:11Z

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PMID:6228057

2012-06-05T19:17:55Z

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{| id="Z4fce5b637b831" class=" tableEdit PMID_info_table"

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!align=left |Citation
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'''Hochman, L, Segev, N, Sternberg, N and Cohen, G''' (1983) Site-specific recombinational circularization of bacteriophage P1 DNA.''Virology'' '''131''':11-7
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!align=left |Abstract
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No abstract in PubMed
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6228057 PubMed]

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!align=left |Keywords
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Bacteriophage lambda; Chloramphenicol; Coliphages; DNA, Circular; DNA, Viral; Escherichia coli; Lysogeny; Recombination, Genetic

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

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{{LitMaterials}}

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<protect>

{| id="J4fce5b6383ad6" class=" tableEdit GO_table_reference dataTable"
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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PMID:6228057

2012-06-05T19:17:55Z

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PMID:6997499

2012-06-05T19:17:43Z

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{| id="A4fce5b56e721f" class=" tableEdit PMID_info_table"

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'''Crawford, JT and Goldberg, EB''' (1980) The function of tail fibers in triggering baseplate expansion of bacteriophage T4.''J. Mol. Biol.'' '''139''':679-90
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!align=left |Abstract
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No abstract in PubMed
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6997499 PubMed]

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!align=left |Keywords
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Centrifugation, Density Gradient; Escherichia coli; Hot Temperature; Mutation; T-Phages; Transfection

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

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{{LitMaterials}}

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<protect>

{| id="P4fce5b571d9bc" class=" tableEdit GO_table_reference dataTable"
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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<references/>

PMID:6997499

2012-06-05T19:17:42Z

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PMID:2061278

2012-06-05T18:52:18Z

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{| id="E4fce5561b1bd0" class=" tableEdit PMID_info_table"

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!align=left |Citation
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'''Brendler, T, Abeles, A and Austin, S''' (1991) Critical sequences in the core of the P1 plasmid replication origin.''J. Bacteriol.'' '''173''':3935-42
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!align=left |Abstract
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The core of the P1 plasmid replication origin consists of a series of 7-bp repeats and a G+C-rich stretch. Methylation of the GATC sequences in the repeats is essential. Forty different single-base mutations in the region were isolated and assayed for origin function. A single-base change within any 7-bp repeat could block the origin, irrespective of whether GATC bases were affected. The repeats themselves were critical, but the short intervals between them were not. Mutations in the G+C-rich region showed it to be a spacer whose exact length is important but whose sequence can vary considerably. It maintains a precise distance between the 7-bp repeats and binding sites for the P1 RepA initiator protein. It may also serve as a clamp to limit strand separation during initiation.
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2061278 PubMed]

|-
!align=left |Keywords
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Bacterial Proteins; Bacteriophages; Base Composition; Base Sequence; DNA Helicases; DNA Replication; DNA, Viral; DNA-Binding Proteins; Methylation; Molecular Sequence Data; Mutagenesis; Phenotype; Plasmids; Proteins; Repetitive Sequences, Nucleic Acid; Replicon; Trans-Activators

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="C4fce556249eed" class=" tableEdit GO_table_reference dataTable"
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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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{{RefHelp}}
<references/>

PMID:2061278

2012-06-05T18:52:17Z

192.31.21.179: New PMID: Page!

PMID:2763457

2012-06-05T17:15:11Z

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{| id="W4fce3e9f08b30" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Kliem, M and Dreiseikelmann, B''' (1989) The superimmunity gene sim of bacteriophage P1 causes superinfection exclusion.''Virology'' '''171''':350-5
|-
!align=left |Abstract
||
Previous work has shown that the sim gene of bacteriophage P1, if cloned into a multicopy vector, confers immunity against P1 infection to cells. We show that a 1.85-kb DNA fragment from the sim region of P1 (in the multicopy plasmid pMK4) expresses immunity and encodes three proteins with molecular weights of about 25, 24, and 15 kDa. Deletion of 650 bp from the sim region abolished synthesis of all three proteins and of the sim phenotype. Expression of sim did not prevent adsorption of P1 to cells. Successful transfection with linear P1 DNA suggests that the recombinational circularization of P1 DNA is not inhibited in the presence of sim. Plasmid pMK4 and a P1 prophage can be stably maintained in the cell indicating that replication of the prophage is not disturbed by sim. The prophage can be induced in the presence of sim. This shows that the sim phenotype is not caused by preventing lytic replication or phage maturation. In cells with pMK4 there is no expression of genes from infecting phages and transduction frequency is drastically reduced. We suggest that sim functions as a superinfection exclusion system by preventing transfer of DNA from the adsorbed phages into the cytoplasm.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2763457 PubMed]

|-
!align=left |Keywords
||
Cloning, Molecular; Coliphages; DNA Mutational Analysis; Gene Expression Regulation; Genes, Viral; Lysogeny; Transduction, Genetic; Viral Proteins; Virus Replication

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182424.W4fce3e9f08b30&page=182424&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="R4fce3e9f0eae8" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182424.R4fce3e9f0eae8&page=182424&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:2763457

2012-06-05T17:15:10Z

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PMID:6312056

2012-06-05T17:15:06Z

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{| id="S4fce3e9a0656e" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Austin, S and Abeles, A''' (1983) Partition of unit-copy miniplasmids to daughter cells. I. P1 and F miniplasmids contain discrete, interchangeable sequences sufficient to promote equipartition.''J. Mol. Biol.'' '''169''':353-72
|-
!align=left |Abstract
||
Hybrids formed by insertion of the plasmid maintenance regions of P1 or F into a lambda delta att vector form stable unit-copy plasmids in their Escherichia coli host. They must therefore both be substrates for an accurate cellular partition apparatus that ensures that all daughter cells inherit a plasmid copy. Analysis of deletion mutants of both types of hybrid showed that, although the P1 and F plasmid maintenance regions differ in sequence and specificity, they are similar in general organization. Each contains an approximately 3 X 10(3) base-pair region that is essential for replication (rep) and an adjacent but separable 3 X 10(3) base-pair region that is essential for the stability of plasmid maintenance (par). Each par region is thought to specify the recognition of the plasmid as a substrate for equipartition. The deletion mutants provide sources of isolated rep and par sequences from both P1 and F DNA. These elements were then used to construct composite plasmids with novel combinations and arrangements of rep and par sequences. Heterologous constructions containing P1 rep and F par or F rep and P1 par sequences were maintained faithfully. We conclude that par regions are both necessary and sufficient to promote equipartition of replicating plasmid DNA. This activity is exerted only in cis but otherwise seems to be independent of the position or orientation of the par sequences within the DNA. Both P1 and F par regions include DNA sequences (incB of P1, incD of F) that we propose are analogues of the centromeres of eukaryotic chromosomes. The remaining portions of the par regions are known to encode protein products that, we believe, act at the inc sites. Extra copies of these inc sites appear to exert incompatibility by competition for the cellular partition apparatus.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6312056 PubMed]

|-
!align=left |Keywords
||
Bacteriophage lambda; Base Sequence; Cell Division; Chromosome Deletion; Coliphages; DNA Replication; DNA Restriction Enzymes; Electrophoresis, Agar Gel; Escherichia coli; F Factor; Lysogeny; Mutation; Plasmids; Recombination, Genetic

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182423.S4fce3e9a0656e&page=182423&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="Q4fce3e9a30dbd" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182423.Q4fce3e9a30dbd&page=182423&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:6312056

2012-06-05T17:15:05Z

192.31.21.179: New PMID: Page!

PMID:9294449

2012-06-05T15:01:31Z

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{{RightTOC}}


{| id="B4fce1f4ae0479" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Arnau, J, Jørgensen, F, Madsen, SM, Vrang, A and Israelsen, H''' (1997) Cloning, expression, and characterization of the Lactococcus lactis pfl gene, encoding pyruvate formate-lyase.''J. Bacteriol.'' '''179''':5884-91
|-
!align=left |Abstract
||
The Lactococcus lactis pfl gene, encoding pyruvate formate-lyase (PFL), has been cloned and characterized. The deduced amino acid sequence of the L. lactis PFL. protein showed high similarity to those of other bacterial PFL proteins and included the conserved glycine residue involved in posttranslational activation of PFL. The genetic organization of the chromosomal pfl region in L. lactis showed differences from other characterized pfl loci, with an upstream open reading frame independently transcribed in the same orientation as the pfl gene. The gene coding for PFL-activase (act), normally found downstream of pfl, was not identified in L. lactis. Analysis of pfl expression showed a strong induction under anaerobiosis at the transcriptional level independent of the growth medium used. During growth with galactose, pfl showed the highest levels of expression. Constructed L. lactis pfl strains were unable to produce formate under anaerobic growth. Higher levels of diacetyl and acetoin were produced anaerobically in the constructed Lactococcus lactis subsp. lactis biovar diacetylactis pfl strain.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9294449 PubMed]

|-
!align=left |Keywords
||
Acetyltransferases; Amino Acid Sequence; Base Sequence; Cloning, Molecular; Gene Expression; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genes, Bacterial; Lactococcus lactis; Molecular Sequence Data; Restriction Mapping; Sequence Alignment; Sequence Homology, Amino Acid; Species Specificity

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182422.B4fce1f4ae0479&page=182422&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="L4fce1f4b3f890" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182422.L4fce1f4b3f890&page=182422&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:9294449

2012-06-05T15:01:30Z

192.31.21.179: New PMID: Page!

User:Obriantkm

2012-05-26T00:07:59Z

192.31.21.179: Fill User: Page!

Click "edit table" in the last row to add information to the table. Click [[Help:Adding_your_information|here]] to find out how to add more information to your user page.



{| id="U4fc01edee95cb" class=" tableEdit User_info_table"

|-
!align=left |Name (Last, First)
||
O'Briant, Kelly
|-
!align=left |Organization
||
Virginia Commonwealth University
|-
!align=left |Position
||

|-
!align=left |Address
||

|-
!align=left |Contact Info
||

|-
!align=left |Website(s)
||

|-
!align=left |Interests
||

|-
!align=left |Genes
||

|-
!align=left |Lab members
||

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182419.U4fc01edee95cb&page=182419&pagename={{FULLPAGENAMEE}}&type=1&template=User_info_table edit table] ||
|}


==References==
{{RefHelp}}
<references/>

User:Obriantkm

2012-05-26T00:07:58Z

192.31.21.179: New User: Page!

PMID:1669732

2012-05-24T15:50:42Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="D4fbe58d26cfba" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Knoll, M''' (1991) [In Maria's life there are others who set the tempo. Interview by Carina Roxström].''Vardfacket'' '''15''':8-15
|-
!align=left |Abstract
||
No abstract in PubMed
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1669732 PubMed]

|-
!align=left |Keywords
||
Adult; Child; Child Rearing; Family; Female; Humans; Life Style; Nurses; Women, Working

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.D4fbe58d26cfba&page=180698&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="X4fbe58d290639" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.X4fbe58d290639&page=180698&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:1669732

2012-05-24T13:59:01Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="T4fbe3ea553384" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Knoll, M''' (1991) [In Maria's life there are others who set the tempo. Interview by Carina Roxström].''Vardfacket'' '''15''':8-15
|-
!align=left |Abstract
||
No abstract in PubMed
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1669732 PubMed]

|-
!align=left |Keywords
||
Adult; Child; Child Rearing; Family; Female; Humans; Life Style; Nurses; Women, Working

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.T4fbe3ea553384&page=180698&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="M4fbe3ea585007" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.M4fbe3ea585007&page=180698&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:1669732

2012-05-24T05:20:12Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="Z4fbdc50c88e8f" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Knoll, M''' (1991) [In Maria's life there are others who set the tempo. Interview by Carina Roxström].''Vardfacket'' '''15''':8-15
|-
!align=left |Abstract
||
No abstract in PubMed
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1669732 PubMed]

|-
!align=left |Keywords
||
Adult; Child; Child Rearing; Family; Female; Humans; Life Style; Nurses; Women, Working

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.Z4fbdc50c88e8f&page=180698&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="I4fbdc50c8e418" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.I4fbdc50c8e418&page=180698&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:1669732

2012-05-24T05:20:11Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="L4fbdc50aeec79" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Knoll, M''' (1991) [In Maria's life there are others who set the tempo. Interview by Carina Roxström].''Vardfacket'' '''15''':8-15
|-
!align=left |Abstract
||
No abstract in PubMed
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1669732 PubMed]

|-
!align=left |Keywords
||
Adult; Child; Child Rearing; Family; Female; Humans; Life Style; Nurses; Women, Working

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.L4fbdc50aeec79&page=180698&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="L4fbdc50b25405" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.L4fbdc50b25405&page=180698&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:1669732

2012-05-21T17:36:43Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="J4fba7d2b28385" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Knoll, M''' (1991) [In Maria's life there are others who set the tempo. Interview by Carina Roxström].''Vardfacket'' '''15''':8-15
|-
!align=left |Abstract
||
No abstract in PubMed
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1669732 PubMed]

|-
!align=left |Keywords
||
Adult; Child; Child Rearing; Family; Female; Humans; Life Style; Nurses; Women, Working

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.J4fba7d2b28385&page=180698&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="Q4fba7d2b47531" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.Q4fba7d2b47531&page=180698&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:1669732

2012-05-21T17:30:34Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="N4fba7bb9af809" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Knoll, M''' (1991) [In Maria's life there are others who set the tempo. Interview by Carina Roxström].''Vardfacket'' '''15''':8-15
|-
!align=left |Abstract
||
No abstract in PubMed
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1669732 PubMed]

|-
!align=left |Keywords
||
Adult; Child; Child Rearing; Family; Female; Humans; Life Style; Nurses; Women, Working

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.N4fba7bb9af809&page=180698&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="K4fba7bb9c086f" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.K4fba7bb9c086f&page=180698&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:1669732

2012-05-21T17:11:25Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="W4fba773d127e4" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Knoll, M''' (1991) [In Maria's life there are others who set the tempo. Interview by Carina Roxström].''Vardfacket'' '''15''':8-15
|-
!align=left |Abstract
||
No abstract in PubMed
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1669732 PubMed]

|-
!align=left |Keywords
||
Adult; Child; Child Rearing; Family; Female; Humans; Life Style; Nurses; Women, Working

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.W4fba773d127e4&page=180698&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="O4fba773d71b6b" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.O4fba773d71b6b&page=180698&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:1669732

2012-05-21T16:55:07Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="B4fba736ae9c19" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Knoll, M''' (1991) [In Maria's life there are others who set the tempo. Interview by Carina Roxström].''Vardfacket'' '''15''':8-15
|-
!align=left |Abstract
||
No abstract in PubMed
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1669732 PubMed]

|-
!align=left |Keywords
||
Adult; Child; Child Rearing; Family; Female; Humans; Life Style; Nurses; Women, Working

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.B4fba736ae9c19&page=180698&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="V4fba736b406b4" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.180698.V4fba736b406b4&page=180698&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:1

2012-05-21T16:38:21Z

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{{RightTOC}}


{| id="P4fba6f7d48457" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Makar, AB, McMartin, KE, Palese, M and Tephly, TR''' (1975) Formate assay in body fluids: application in methanol poisoning.''Biochem Med'' '''13''':117-26
|-
!align=left |Abstract
||
No abstract in PubMed
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1 PubMed]

|-
!align=left |Keywords
||
Aldehyde Oxidoreductases; Animals; Body Fluids; Carbon Dioxide; Formates; Haplorhini; Humans; Hydrogen-Ion Concentration; Kinetics; Methanol; Methods; Pseudomonas

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.176461.P4fba6f7d48457&page=176461&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="F4fba6f7da59c4" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.176461.F4fba6f7da59c4&page=176461&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:3003084

2012-05-21T16:37:42Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="W4fba6f559141e" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Ohki, M, Tamura, F, Nishimura, S and Uchida, H''' (1986) Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product.''J. Biol. Chem.'' '''261''':1778-81
|-
!align=left |Abstract
||
The dnaJ and dnaK genes are essential for replication of Escherichia coli DNA, and they constitute an operon, dnaJ being downstream from dnaK. The amount of the dnaJ protein in E. coli is substantially less than that of the dnaK protein, which is produced abundantly. In order to construct a system that over-produces the dnaJ protein, we started our study by determining the DNA sequence of the entire dnaJ gene, and an operon fusion was constructed by inserting the gene downstream of the lambda PL promoter of an expression vector plasmid, pPL-lambda. Cells containing the recombinant plasmid produced dnaJ protein amounting to 2% of the total cellular protein when cells were induced. The overproduced protein was purified, and Edman degradation of the protein indicated that the NH2-terminal methionine was found to be processed. From the DNA sequence of the dnaJ gene, the processed gene product is composed of 375 amino acid residues, and its molecular weight is calculated to be 40,975.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3003084 PubMed]

|-
!align=left |Keywords
||
Amino Acid Sequence; Bacterial Proteins; Base Sequence; DNA Replication; Escherichia coli; Escherichia coli Proteins; Genes, Bacterial; HSP40 Heat-Shock Proteins; Heat-Shock Proteins; Methionine; Molecular Weight; Recombinant Proteins; Sequence Homology, Nucleic Acid

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182400.W4fba6f559141e&page=182400&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="I4fba6f5630a8b" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182400.I4fba6f5630a8b&page=182400&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:3003084

2012-05-21T16:37:41Z

192.31.21.179: New PMID: Page!

Category:GO:0042884 ! microcin transport

2012-05-21T16:11:56Z

192.31.21.179: New GO term category page

{{GO term stub|GO:0042884_!_microcin_transport}}

==References==
{{RefHelp}}
<references/>

PMID:6378205

2012-05-21T15:02:51Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="U4fba591a65918" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Kondo, K, Wakabayashi, S, Yagi, T and Kagamiyama, H''' (1984) The complete amino acid sequence of aspartate aminotransferase from Escherichia coli: sequence comparison with pig isoenzymes.''Biochem. Biophys. Res. Commun.'' '''122''':62-7
|-
!align=left |Abstract
||
The amino acid sequence of aspartate aminotransferase from E. coli B was determined by the alignment of seven cyanogen bromide peptides. The established sequence of the subunit was composed of 396 amino acid residues, and the molecular weight was calculated to be 43,573. The sequence was compared with those of the pig cytoplasmic and mitochondrial isoenzymes, showing that nearly 30% of all residues were invariant and that the E. coli enzyme exhibited the same degree of homology (about 40%) with either of them. Although majority of the residues were substituted, the functional residues constituting the active site structure were conserved.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6378205 PubMed]

|-
!align=left |Keywords
||
Amino Acid Sequence; Animals; Aspartate Aminotransferases; Catalysis; Chemical Phenomena; Chemistry; Cytosol; Escherichia coli; Isoenzymes; Mitochondria, Heart; Myocardium; Species Specificity; Swine

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182398.U4fba591a65918&page=182398&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="Y4fba591b13ff6" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182398.Y4fba591b13ff6&page=182398&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:6378205

2012-05-21T15:02:50Z

192.31.21.179: New PMID: Page!

PMID:387032

2012-05-21T15:02:37Z

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{{RightTOC}}


{| id="J4fba590bdc482" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Kagamiyama, H and Yagi, T''' (1979) Aspartate transaminase from E. coli: amino acid sequences of the NH2-terminal 33 residues and chymotryptic pyridoxyl tetrapeptide.''Biochem. Biophys. Res. Commun.'' '''89''':1347-53
|-
!align=left |Abstract
||
No abstract in PubMed
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=387032 PubMed]

|-
!align=left |Keywords
||
Amino Acid Sequence; Animals; Aspartate Aminotransferases; Chymotrypsin; Cytosol; Escherichia coli; Mitochondria, Heart; Myocardium; Peptide Fragments; Pyridoxal; Species Specificity; Swine

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182397.J4fba590bdc482&page=182397&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="O4fba590cc5a44" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182397.O4fba590cc5a44&page=182397&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:387032

2012-05-21T15:02:35Z

192.31.21.179: New PMID: Page!

Category:GO:0044419 ! interspecies interaction between organisms

2012-05-21T13:37:12Z

192.31.21.179: New GO term category page

{{GO term stub|GO:0044419_!_interspecies_interaction_between_organisms}}

==References==
{{RefHelp}}
<references/>

PMID:384209

2012-05-21T13:14:14Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="N4fba3fa11e7bb" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Raychowdhury, R, Schlesinger, DH and Wilson, DB''' (1979) E. coli galactose-1-phosphate uridyl transferase: N-terminal and C-terminal sequences.''Mol. Cell. Biochem.'' '''23''':167-75
|-
!align=left |Abstract
||
A modified procedure for the purification of E. coli galactose-1-phosphate uridyl transferase (E.C. 2.7.6.12) was developed which reproducibly gives pure enzyme. The purified enzyme was shown to be a dimeric protein with a subunit molecular weight of 41,000 and its amino acid composition and content of free sulfhydryl groups were determined. The N-terminal and C-terminal amino acid sequences were found to be NH2-thr-gln-phe-asn-pro-val-asp and -ser(val leu)-ala-COOH respectively. This N-terminal sequence allowed the identification of the start of the transferase gene in the DNA sequence determined by GRINDLEY. Furthermore it appears to define a nine base intercistronic region between the epimerase and transferase genes.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=384209 PubMed]

|-
!align=left |Keywords
||
Amino Acid Sequence; Amino Acids; Escherichia coli; Methods; Molecular Weight; Nucleotidyltransferases; Operon; UTP-Hexose-1-Phosphate Uridylyltransferase

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182395.N4fba3fa11e7bb&page=182395&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="F4fba3fa5cb238" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182395.F4fba3fa5cb238&page=182395&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:384209

2012-05-21T13:14:07Z

192.31.21.179: New PMID: Page!

PMID:2649968

2012-05-21T12:55:39Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="H4fba3b4a6239c" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Escherich, T and Escherich, T''' The intestinal bacteria of the neonate and breast-fed infant. 1885.''Rev. Infect. Dis.'' '''11''':352-6
|-
!align=left |Abstract
||
No abstract in PubMed
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2649968 PubMed]

|-
!align=left |Keywords
||
Bacteria; Bacteriology; Breast Feeding; History, 19th Century; Humans; Infant, Newborn; Intestines

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182394.H4fba3b4a6239c&page=182394&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="I4fba3b4b8b3ce" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182394.I4fba3b4b8b3ce&page=182394&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>

PMID:2649968

2012-05-21T12:55:36Z

192.31.21.179: New PMID: Page!

PMID:17085577

2012-05-20T21:27:07Z

192.31.21.179: Fill PMID: Page!

{{RightTOC}}


{| id="J4fb961aa4d0eb" class=" tableEdit PMID_info_table"

|-
!align=left |Citation
||
'''Shiomi, D and Margolin, W''' (2007) The C-terminal domain of MinC inhibits assembly of the Z ring in Escherichia coli.''J. Bacteriol.'' '''189''':236-43
|-
!align=left |Abstract
||
In Escherichia coli, the Min system, consisting of three proteins, MinC, MinD, and MinE, negatively regulates FtsZ assembly at the cell poles, helping to ensure that the Z ring will assemble only at midcell. Of the three Min proteins, MinC is sufficient to inhibit Z-ring assembly. By binding to MinD, which is mostly localized at the membrane near the cell poles, MinC is sequestered away from the cell midpoint, increasing the probability of Z-ring assembly there. Previously, it has been shown that the two halves of MinC have two distinct functions. The N-terminal half is sufficient for inhibition of FtsZ assembly, whereas the C-terminal half of the protein is required for binding to MinD as well as to a component of the division septum. In this study, we discovered that overproduction of the C-terminal half of MinC (MinC(122-231)) could also inhibit cell division and that this inhibition was at the level of Z-ring disassembly and dependent on MinD. We also found that fusing green fluorescent protein to either the N-terminal end of MinC(122-231), the C terminus of full-length MinC, or the C terminus of MinC(122-231) perturbed MinC function, which may explain why cell division inhibition by MinC(122-231) was not detected previously. These results suggest that the C-terminal half of MinC has an additional function in the regulation of Z-ring assembly.
|-
!align=left |Links
||
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=17085577 PubMed]
Online version:[http://dx.doi.org/10.1128/JB.00666-06 10.1128/JB.00666-06]
|-
!align=left |Keywords
||
Adenosine Triphosphatases; Cell Division; Escherichia coli; Escherichia coli Proteins; Membrane Proteins; Protein Structure, Tertiary

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182393.J4fb961aa4d0eb&page=182393&pagename={{FULLPAGENAMEE}}&type=1&template=PMID_info_table edit table] ||
|}


==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
{{LitMaterials}}

==Annotations {{HelpIcon|Help:Literature}}==
<protect>

{| id="Y4fb961ab862a2" class=" tableEdit GO_table_reference dataTable"
|- {{table heading style}}
!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

|- class="tableEdit_footer"
|[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d41d8cd98f00b204e9800998ecf8427e.182393.Y4fb961ab862a2&page=182393&pagename={{FULLPAGENAMEE}}&type=0&template=GO_table_reference edit table] || || || || || || || ||
|}
</protect>

==EcoliWiki Links {{HelpIcon|Help:Literature}} ==
{{LitLinks}}

==See also==

==References==
{{RefHelp}}
<references/>