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2026-11-15T12:48:26Z
User contributions
MediaWiki 1.43.11
https://ecoliwiki.org/colipedia/index.php?title=PMID:148458&diff=1491796
PMID:148458
2014-11-13T06:37:23Z
<p>188.143.232.123: Fill PMID: Page!</p>
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'''Hochhauser, SJ and Weiss, B''' (1978) Escherichia coli mutants deficient in deoxyuridine triphosphatase. ''J. Bacteriol.'' '''134''':157-66<br />
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!align=left |Abstract<br />
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Mutants deficient in deoxyuridine triphosphatase (dUTPase) were identified by enzyme assays of randomly chosen heavily mutagenized clones. Five mutants of independent origin were obtained. One mutant produced a thermolabile enzyme, and it was presumed to have a mutation in the structural gene for dUTPase, designated dut. The most deficient mutant had the following associated phenotypes: less than 1% of parental dUTPase activity, prolonged generation time, increased sensitivity to 5'-fluorodeoxyuridine, increased rate of spontaneous mutation, increased rate of recombination (hyper-Rec), an inhibition of growth in the presence of 2 mM uracil, and a decreased ability to support the growth of phage P1 (but not T4 or lambda). This mutation also appeared to be incompatible with pyrE mutations. A revertant selected by its faster growth had regained dUTPase activity and lost its hyper-Rec phenotype. Many of the properties of the dut mutants are compatible with their presumed increased incorporation of uracil into DNA and the subsequent transient breakage of the DNA by excision repair.<br />
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=148458 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC222230 PMC222230]<br />
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!align=left |Keywords<br />
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Coliphages/genetics; Coliphages/growth & development; Deoxyuracil Nucleotides; Escherichia coli/enzymology; Escherichia coli/genetics; Floxuridine/pharmacology; Genes; Genetic Linkage; Mutation; Pyrophosphatases/biosynthesis; Recombination, Genetic; Transduction, Genetic; Uracil/pharmacology<br />
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==Significance {{HelpIcon|Help:Literature}} ==<br />
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188.143.232.123
https://ecoliwiki.org/colipedia/index.php?title=PMID:148458&diff=1491795
PMID:148458
2014-11-13T06:37:22Z
<p>188.143.232.123: New PMID: Page!</p>
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188.143.232.123
https://ecoliwiki.org/colipedia/index.php?title=PMID:402918&diff=1491794
PMID:402918
2014-11-13T05:11:19Z
<p>188.143.232.123: Fill PMID: Page!</p>
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{| id="H54643d7732d7b" class=" tableEdit PMID_info_table" <br />
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'''Smith, MR and Cook, RT''' (1977) Urea reduces the thermal stability of polyanion-treated chromatin. ''Biochem. Biophys. Res. Commun.'' '''74''':1475-82<br />
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!align=left |Abstract<br />
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No abstract in PubMed<br />
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=402918 PubMed]<br />
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!align=left |Keywords<br />
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Animals; Binding Sites; Chromatin/metabolism; Drug Stability; Edetic Acid; Glutamates; Heparin; Hot Temperature; Liver/metabolism; Male; Molecular Weight; Nucleic Acid Conformation; Nucleic Acid Denaturation; Peptides; Protein Binding; Rats; Urea<br />
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==Significance {{HelpIcon|Help:Literature}} ==<br />
{{LitSignificance}}<br />
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== Useful Materials and Methods {{HelpIcon|Help:Literature}}==<br />
{{LitMaterials}}<br />
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==Annotations {{HelpIcon|Help:Literature}}==<br />
<annotationlinks/><br />
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==EcoliWiki Links {{HelpIcon|Help:Literature}} ==<br />
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==See also==<br />
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==References==<br />
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<references/></div>
188.143.232.123
https://ecoliwiki.org/colipedia/index.php?title=PMID:402918&diff=1491793
PMID:402918
2014-11-13T05:11:19Z
<p>188.143.232.123: New PMID: Page!</p>
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188.143.232.123