PMID:19706412

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!align=left |Citation
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'''Peters, JM, Mooney, RA, Kuan, PF, Rowland, JL, Keles, S and Landick, R''' (2009) Rho directs widespread termination of intragenic and stable RNA transcription.''Proc. Natl. Acad. Sci. U.S.A.'' '''106''':15406-11
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!align=left |Abstract
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The transcription termination factor Rho is a global regulator of RNA polymerase (RNAP). Although individual Rho-dependent terminators have been studied extensively, less is known about the sites of RNAP regulation by Rho on a genome-wide scale. Using chromatin immunoprecipitation and microarrays (ChIP-chip), we examined changes in the distribution of Escherichia coli RNAP in response to the Rho-specific inhibitor bicyclomycin (BCM). We found approximately 200 Rho-terminated loci that were divided evenly into 2 classes: intergenic (at the ends of genes) and intragenic (within genes). The intergenic class contained noncoding RNAs such as small RNAs (sRNAs) and transfer RNAs (tRNAs), establishing a previously unappreciated role of Rho in termination of stable RNA synthesis. The intragenic class of terminators included a previously uncharacterized set of short antisense transcripts, as judged by a shift in the distribution of RNAP in BCM-treated cells that was opposite to the direction of the corresponding gene. These Rho-terminated antisense transcripts point to a role of noncoding transcription in E. coli gene regulation that may resemble the ubiquitous noncoding transcription recently found to play myriad roles in eukaryotic gene regulation.
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19706412 PubMed]
Online version:[http://dx.doi.org/10.1073/pnas.0903846106 10.1073/pnas.0903846106]
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!align=left |Keywords
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Bicyclo Compounds, Heterocyclic; Chromatin Immunoprecipitation; DNA-Directed RNA Polymerases; Escherichia coli; Genes, Bacterial; Microarray Analysis; RNA; Transcription, Genetic; rho GTP-Binding Proteins

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!|Gene product!!Qualifier!!GO ID!!GO term name!!Evidence Code!!with/from!!Aspect!!Notes!!Status

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PMID:19706412

2012-11-21T18:12:32Z

152.16.223.158: New PMID: Page!

EcoliWiki - User contributions [en]

PMID:19706412

PMID:19706412