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<span style = 'font-size: large'> '''Wang, Q, Ou, MS, Kim, Y, Ingram, LO and Shanmugam, KT''' (2010) Metabolic Flux Control at the Pyruvate Node in an Anaerobic Escherichia coli with Active Pyruvate Dehydrogenase.''Appl Environ Microbiol'' </span><br />
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|{{TableHeadingColor}} |'''Abstract''' || <protect>During anaerobic growth of Escherichia coli, pyruvate formate-lyase (PFL) and lactate dehydrogenase (LDH) channel pyruvate towards a mixture of fermentation products. We have introduced a third branch at the pyruvate node in a mutant of E. coli with a mutation in pyruvate dehydrogenase (PDH*) that renders the enzyme less sensitive to inhibition by NADH. The key starting enzymes of the three branches at the pyruvate node in such a mutant, PDH*, PFL and LDH, have different metabolic potential and kinetic properties. In such a mutant (strain QZ2), pyruvate flux through LDH was about 30% with the remainder through PFL indicating that LDH is a preferred route of pyruvate conversion over PDH*. In a pfl mutant (strain YK167) with both PDH* and LDH activities, flux through PDH* was about 33% of the total confirming the ability of LDH to outcompete the PDH pathway for pyruvate in vivo. Only in the absence of LDH (strain QZ3), pyruvate carbon was equally distributed between PDH* and PFL pathways. A pfl mutant with LDH and PDH* activities as well as a pfl, ldh double mutant with PDH* activity had a surprisingly low YATP (about 7.0 grams cells per mole ATP ) compared to 10.9 grams cells per mole ATP for the wild type. The lower YATP suggests the operation of a futile energy cycle in the absence of PFL in this strain. An understanding of the controls at the pyruvate node during anaerobic growth is expected to provide unique insights into rational metabolic engineering of E. coli and related bacteria for production of various bio-based products at high rate and yield.</protect><br />
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|{{TableHeadingColor}} nowrap |'''Links''' || [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=20118372 PubMed]<br/>Online version:[http://dx.doi.org/10.1128/AEM.02545-09 10.1128/AEM.02545-09]<br />
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<references/></div>128.227.41.137https://ecoliwiki.org/colipedia/index.php?title=PMID:20118372&diff=1061142PMID:201183722010-02-26T22:29:15Z<p>128.227.41.137: New PMID: Page!</p>
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