https://ecoliwiki.org/colipedia/api.php?action=feedcontributions&feedformat=atom&user=128.219.49.13EcoliWiki - User contributions [en]2026-11-05T23:26:23ZUser contributionsMediaWiki 1.43.11https://ecoliwiki.org/colipedia/index.php?title=PMID:7783622&diff=1491012PMID:77836222014-05-12T17:00:54Z<p>128.219.49.13: Fill PMID: Page!</p>
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'''Quail, MA and Guest, JR''' (1995) Purification, characterization and mode of action of PdhR, the transcriptional repressor of the pdhR-aceEF-lpd operon of Escherichia coli. ''Mol. Microbiol.'' '''15''':519-29<br />
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!align=left |Abstract<br />
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The repressor of the pdhR-aceEF-lpd operon of Escherichia coli, PdhR, was amplified to 23% of total cell protein and purified to homogeneity by heparin-agarose and cation-exchange chromatography. The purified protein is a monomer (M(r) 29,300) which binds specifically to DNA fragments containing the pdh promoter (Ppdh) in the absence of pyruvate. The pdh operator was identified by DNase I footprinting as a region of hyphenated dyad symmetry, +11AATTGGTaagACCAATT+27, situated just downstream of the transcript start site. In vitro transcription from Ppdh was repressed > 1000-fold by PdhR and this repression was antagonized in a concentration-dependent manner by its co-effector, pyruvate. Studies on RNA polymerase binding at Ppdh showed that RNA polymerase protects the -44 to +21 region in the absence of PdhR, but no RNA polymerase binding or protection upstream of +9 could be detected in the presence of PdhR. It is concluded that PdhR represses transcription by binding to an operator site centred at +19 such that effective binding of RNA polymerase is prevented.<br />
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7783622 PubMed]<br />
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!align=left |Keywords<br />
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Acetyltransferases/biosynthesis; Acetyltransferases/genetics; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Base Sequence; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; DNA-Directed RNA Polymerases/metabolism; Dihydrolipoamide Dehydrogenase/biosynthesis; Dihydrolipoamide Dehydrogenase/genetics; Dihydrolipoyllysine-Residue Acetyltransferase; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression Regulation, Bacterial/drug effects; Ketoglutarate Dehydrogenase Complex/biosynthesis; Ketoglutarate Dehydrogenase Complex/genetics; Molecular Sequence Data; Operon/genetics; Pyruvate Dehydrogenase Complex/biosynthesis; Pyruvate Dehydrogenase Complex/genetics; Pyruvates; Pyruvic Acid; Recombinant Fusion Proteins/metabolism; Repressor Proteins/genetics; Repressor Proteins/isolation & purification; Transcription Factors/genetics; Transcription Factors/isolation & purification<br />
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<references/></div>128.219.49.13https://ecoliwiki.org/colipedia/index.php?title=PMID:7783622&diff=1491011PMID:77836222014-05-12T17:00:53Z<p>128.219.49.13: New PMID: Page!</p>
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