EcoliWiki - User contributions [en]

PMID:3056915

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'''Luisi-DeLuca, C, Clark, AJ and Kolodner, RD''' (1988) Analysis of the recE locus of Escherichia coli K-12 by use of polyclonal antibodies to exonuclease VIII. ''J. Bacteriol.'' '''170''':5797-805
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!align=left |Abstract
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Exonuclease VIII (exoVIII) of Escherichia coli has been purified from a strain carrying a plasmid-encoded recE gene by using a new procedure. This procedure yielded 30 times more protein per gram of cells, and the protein had a twofold higher specific activity than the enzyme purified by the previously published procedure (J. W. Joseph and R. Kolodner, J. Biol. Chem. 258:10411-10417, 1983). The sequence of the 12 N-terminal amino acids was also obtained and found to correspond to one of the open reading frames predicted from the nucleic acid sequence of the recE region of Rac (C. Chu, A. Templin, and A. J. Clark, manuscript in preparation). Polyclonal antibodies directed against purified exoVIII were also prepared. Cell-free extracts prepared from strains containing a wide range of chromosomal- or plasmid-encoded point, insertion, and deletion mutations which result in expression of exoVIII were examined by Western blot (immunoblot) analysis. This analysis showed that two point sbcA mutations (sbcA5 and sbcA23) and the sbc insertion mutations led to the synthesis of the 140-kilodalton (kDa) polypeptide of wild-type exoVIII. Plasmid-encoded partial deletion mutations of recE reduced the size of the cross-reacting protein(s) in direct proportion to the size of the deletion, even though exonuclease activity was still present. The analysis suggests that 39 kDa of the 140-kDa exoVIII subunit is all that is essential for exonuclease activity. One of the truncated but functional exonucleases (the pRAC3 exonuclease) has been purified and confirmed to be a 41-kDa polypeptide. The first 18 amino acids from the N terminus of the 41-kDa pRAC3 exonuclease were sequenced and fond to correspond to one of the translational start signals predicted from the nucleotide sequence of radC (Chu et al., in preparation).
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3056915 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC211685 PMC211685]

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!align=left |Keywords
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Alleles; Amino Acid Sequence; Antibodies; Antigen-Antibody Complex/analysis; Base Sequence; Blotting, Western; Chromosome Deletion; Escherichia coli/enzymology; Escherichia coli/genetics; Exodeoxyribonucleases/genetics; Exodeoxyribonucleases/immunology; Exodeoxyribonucleases/isolation & purification; Genes; Genes, Bacterial; Molecular Sequence Data; Nucleic Acid Hybridization; Plasmids

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PMID:3056915

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PMID:6257642

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'''Gillen, JR, Willis, DK and Clark, AJ''' (1981) Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12. ''J. Bacteriol.'' '''145''':521-32
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!align=left |Abstract
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The RecE pathway of genetic recombination in Escherichia coli K-12 was defined to be the pathway that is utilized in deoxyribonucleic acid exonuclease V (ExoV)-defective cells which express constitutively recE+, the structural gene for deoxyribonucleic acid exonuclease VIII. Dependence on ExoVIII was shown by the occurrence in a recB21 sbcA23 strain of recombination deficiency mutations in recE, the structural gene for ExoVIII. Point mutations in recE were found as well as deletion mutations in which the entire Rac prophage, carrying recE, was lost. In addition, strain construction and mutagenesis revealed the dependence of the RecE pathway on recA+ and on recF+. Dependence on a fourth gene was shown by a mutation (rec-77) which does not map near the other genes. The problem of distinguishing the RecE pathway from that previously called RecF is discussed.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6257642 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC217302 PMC217302]

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!align=left |Keywords
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Escherichia coli/genetics; Exonucleases/genetics; Genes; Mutation; Recombination, Genetic

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PMID:6257642

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PMID:4860192

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'''Gellert, M''' (1967) Formation of covalent circles of lambda DNA by E. coli extracts. ''Proc. Natl. Acad. Sci. U.S.A.'' '''57''':148-55
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No abstract in PubMed
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4860192 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC335477 PMC335477]

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Centrifugation, Density Gradient; Coliphages; DNA, Viral/biosynthesis; Escherichia coli/metabolism; Microscopy, Electron; Molecular Biology; Mutation

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PMID:4860192

2013-08-01T14:01:30Z

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PMID:13769766

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'''MESELSON, M and WEIGLE, JJ''' (1961) Chromosome brekage accompanying genetic recombination in bacteriophage. ''Proc. Natl. Acad. Sci. U.S.A.'' '''47''':857-68
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!align=left |Abstract
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No abstract in PubMed
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=13769766 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC221352 PMC221352]

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!align=left |Keywords
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Bacteriophages/genetics; Chromosomes

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==Significance {{HelpIcon|Help:Literature}} ==
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PMID:13769766

2013-08-01T14:01:05Z

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Category:GO:0006412 ! protein biosynthesis

2013-08-01T13:53:53Z

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{{GO term stub|GO:0006412_!_protein_biosynthesis}}

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PMID:17247494

2013-08-01T13:27:23Z

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'''Appleyard, RK''' (1954) Segregation of Lambda Lysogenicity during Bacterial Recombination in Escherichia Coli K12. ''Genetics'' '''39''':429-39
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No abstract in PubMed
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=17247494 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1209663 PMC1209663]

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

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PMID:17247494

2013-08-01T13:27:23Z

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PMID:3520484

2013-07-22T08:28:07Z

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'''Finch, PW, Wilson, RE, Brown, K, Hickson, ID, Tomkinson, AE and Emmerson, PT''' (1986) Complete nucleotide sequence of the Escherichia coli recC gene and of the thyA-recC intergenic region. ''Nucleic Acids Res.'' '''14''':4437-51
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!align=left |Abstract
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The nucleotide sequence of a 6,000 bp region of the E. coli chromosome that includes the 3' end of the coding region for the thyA gene and the entire recC gene has been determined. The proposed coding region for the RecC protein is 3369 nucleotides long, which would encode a polypeptide consisting of 1122 amino acids with a calculated molecular mass of 129 kDa. Mung bean nuclease mapping of a recC specific transcript produced in vivo indicates that transcription of recC is initiated 80 bp upstream of the translational start point. A weak promoter sequence situated 5' to the transcription initiation point has been identified. In the 1953 bp thyA-recC intergenic region there are three open reading frames that would code for polypeptides of molecular mass 30 kDa, 13.5 kDa and 12 kDa, respectively. Although the first and third of these open reading frames are preceded by possible ribosome binding sites, no obvious promoter sequences could be identified. Moreover, transcripts for these reading frames could not be detected.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3520484 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC311457 PMC311457]

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!align=left |Keywords
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Amino Acid Sequence; Base Sequence; Codon; DNA, Bacterial/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Exodeoxyribonuclease V; Exodeoxyribonucleases/genetics; Genes; Genes, Bacterial; Genetic Linkage; Promoter Regions, Genetic; Recombination, Genetic; Thymine; Transcription, Genetic

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

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PMID:3520484

2013-07-22T08:28:06Z

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PMID:4754844

2013-07-22T08:24:28Z

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'''Lobban, PE and Kaiser, AD''' (1973) Enzymatic end-to end joining of DNA molecules. ''J. Mol. Biol.'' '''78''':453-71
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!align=left |Abstract
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No abstract in PubMed
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4754844 PubMed]

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!align=left |Keywords
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Centrifugation, Density Gradient; DNA; DNA Nucleotidyltransferases; DNA Repair; DNA, Circular/isolation & purification; DNA, Viral; Deoxyribonucleosides; Exonucleases; Microscopy, Electron; Phosphorus Isotopes; Salmonella Phages

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
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PMID:4754844

2013-07-22T08:24:28Z

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PMID:4587405

2013-07-22T08:11:28Z

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{| id="G51ece93094ace" class=" tableEdit PMID_info_table"

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'''Horii, Z and Clark, AJ''' (1973) Genetic analysis of the recF pathway to genetic recombination in Escherichia coli K12: isolation and characterization of mutants. ''J. Mol. Biol.'' '''80''':327-44
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!align=left |Abstract
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No abstract in PubMed
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!align=left |Links
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4587405 PubMed]

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!align=left |Keywords
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Chromosome Mapping; Crosses, Genetic; Escherichia coli/isolation & purification; Escherichia coli/radiation effects; Genotype; Mutation; Nitrosoguanidines; Phenotype; Radiation Effects; Recombination, Genetic; Transduction, Genetic; Ultraviolet Rays

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==Significance {{HelpIcon|Help:Literature}} ==
{{LitSignificance}}

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PMID:4587405

2013-07-22T08:11:28Z

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PMID:6286604

2013-07-22T08:03:19Z

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{| id="U51ece746b0cc3" class=" tableEdit PMID_info_table"

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'''Tessman, I, Fassler, JS and Bennett, DC''' (1982) Relative map location of the rep and rho genes of Escherichia coli. ''J. Bacteriol.'' '''151''':1637-40
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!align=left |Abstract
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The rep gene of Escherichia coli was mapped between ilvC and rho by three-factor P1 transductional crosses and also by complementation with a set of lambda transducing phages that contain known amounts of bacterial DNA linked to ilvC. The physical distance between ilvC and rep and between rep and rho were calculated with an accuracy of +/- 0.4 kilobase to be 0 less than or equal to ilvC-rep less than or equal to 3.4 kilobases and 2.0 less than or equal to rep-rho less than or equal to 6.0 kilobases. It was shown that rho-15 is Gro+ for phage ST-1. An ilv::Tn10 mutation was located in ilvY.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6286604 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC220455 PMC220455]

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!align=left |Keywords
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Bacterial Proteins/genetics; Chromosome Mapping; Chromosomes, Bacterial; DNA Helicases/genetics; Escherichia coli/genetics; Genes, Bacterial; Genetic Complementation Test; Isoleucine/biosynthesis; Leucine/biosynthesis; Rho Factor/genetics; Transcription Factors/genetics; Transduction, Genetic; Valine/biosynthesis

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==Significance {{HelpIcon|Help:Literature}} ==
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== Useful Materials and Methods {{HelpIcon|Help:Literature}}==
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PMID:6286604

2013-07-22T08:03:18Z

121.189.37.60: New PMID: Page!