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Lugtenberg, EJ and v Schijndel-van Dam, A (1972) Temperature-sensitive mutants of Escherichia coli K-12 with low activities of the L-alanine adding enzyme and the D-alanyl-D-alanine adding enzyme. J. Bacteriol. 110:35-40
A number of properties of temperature-sensitive mutants in murein synthesis are described. The mutants grow at 30 C but lyse at 42 C. One mutant possesses a temperature-sensitive d-alanyl-d-alanine adding enzyme, has an impaired rate of murein synthesis in vivo at both 30 and 42 C, and contains elevated levels of uridine diphosphate-N-acetyl-muramyl-tripeptide (UDP-MurNAc-l-Ala-d-Glu-m-diaminopimelic acid) at 42 C. The other mutant possesses an l-alanine adding enzyme with a very low in vitro activity at both 30 and 42 C. Its in vivo rate of murein synthesis is almost normal at 30 C but is much less at 42 C. When the murein precursors were isolated after incubation of the cells in the presence of (14)C-l-alanine, they contained only a fraction of the radioactivity that could be obtained from a wild-type strain. A genetic nomenclature for genes concerned with murein synthesis is proposed.
Acetates; Alanine/metabolism; Carbon Isotopes; Chloramphenicol/pharmacology; Dipeptides; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli/metabolism; Genes; Genetics, Microbial; Glucosamine/biosynthesis; Ligases; Mutation; Nucleoside Diphosphate Sugars/biosynthesis; Peptide Biosynthesis; Peptidoglycan/biosynthesis; Temperature; Time Factors; Uracil Nucleotides